Chemosensitization of murine fibrosarcoma cells to drugs affected by the multidrug resistance phenotype by the antidepressant trazodone - an experimental-model for the reversal of intrinsic drug-resistance

A variety of resistance phenotypes to cytotoxic agents in bacteria, protozoa parasites and mammalian cells are mediated by evolutionarily conserved proteins of the mdr family. The finding that chloroquine resistance in the malarial parasite, Plasmodium falciparum, that is mediated by an mdr-1 gene product can be circumvented by tricyclic antidepressant drugs has stimulated the present study to assess whether this class of agents might also modulate the multidrug resistance (MDR) phenotype(s) in mammalian tumor cells. The possible chemosensitizing effects of nine antidepressant drugs have been tested against the UV-2237M murine fibrosarcoma line and its MDR variant. At nontoxic concentrations all nine antidepressants markedly enhanced the cytotoxicity of ADR against the parental cells but were much less effective against the MDR cells. The most active antidepressant, trazodone, also enhanced the cytotoxicities of vinblastine and vincristine, but not those of actinomycin D, mitomycin C, or 5-fluorouracil. The parental cells treated with trazodone exhibited an increased accumulation of intracellular ADR, but lacked detectable alterations in the expression and drug-binding activity of plasma membrane P-glycoprotein, and trazodone did not affect the activities of isolated protein kinase C and calmodulin. These data suggest that the antidepressant drug trazodone may be useful in the reversal of the intrinsic drug resistance of tumor cells that express low levels of P-glycoprotein.     

清开灵注射液雾化吸入治疗慢性咽炎64例

慢性咽炎是咽粘膜、粘膜下及淋巴组织的慢性炎症，其病因复杂，临床极为常见。笔者于2000年4月-2002年6月采用清开灵注射液雾化吸入治疗慢性咽炎64例，收到满意效果，现报告如下。     

Dietary plasma protein reduces small intestinal growth and lamina propria cell density in early weaned pigs

ABSTRACT We quantified the effects of a diet containing animal plasma protein on small intestinal growth and mucosal morphology in early weaned pigs. Ninety-six pigs [14 d old, 4 kg body weight (BW)] were assigned in groups of 32 to three dietary treatments as follows: 1) free access to control diet (C), 2) free access to plasma protein diet (P), and 3) plasma protein, pair-fed to C (PPF). Eight pigs from each group were killed at 2, 4, 8 or 16 d. Over a 16-d period, weight gain in the P group was 43% greater (P < 0.05) than that in C pigs; weight gain was similar in C and PPF groups. Protein intake in the P group was 33% higher (P < 0.05) than that in the PPF group; no significant difference was observed between the C and P groups. Dietary protein conversion efficiencies in both the P and PPF groups were approximately 18% greater (P < 0.05) than those in the C group. Intestinal masses in the three groups did not differ at 2, 4 and 8 d. By 16 d, the jejunal and ileal protein and DNA masses (mg/kg BW) in both the P and PPF groups were lower than those in the C group (P < 0.05). Dietary plasma protein did not affect crypt cell proliferation, crypt depth or villous height in either the jejunum or ileum. However, the intravillous lamina propria cell density in the jejunum was significantly lower (P < 0.05) in P and PPF pigs than in C pigs. Plasma urea concentrations were also 40 and 42% lower (P < 0.05) in the P and PPF groups, respectively, than in the C group. Our results indicate that dietary plasma protein reduces the cellularity of the lamina propria, but not epithelial cell surface of the small intestine. Feeding plasma protein also increased the efficiency of dietary protein utilization, in part, by decreasing amino acid catabolism.     

不同产地女贞子中水溶性活性成分及齐墩果酸的反相高效液相色谱分析

采用反相高效液相色谱法同时测定了不同产地的女贞子中对羟基苯乙醇-β-Ｄ-葡萄糖甙、特女贞甙和齐墩果酸的含量。提出了全面评价中药女贞子的质量的依据。     

局部封闭加中药外洗治疗肩周炎60例体会

笔者自１９８３年以来，用局部封闭加中药治疗肩周炎６０例，疗效满意，现报告如下。一般资料本组６０例中男性２５例，女性３５例；年龄３５～６５岁，其中３５～４５岁２０例，４６～６５岁４０例；病程６个月以内者２２例，６个月～１年者２８例，１年以上者１０例；单...     

Gene therapy of Parkinson's disease using adeno-associated virus (AAV) vectors

Parkinson's disease (PD) is characterized by the progressive loss of the dopaminergic neurons in the substantia nigra and a severe decrease in dopamine in the striatum. A promising approach to the gene therapy of PD is intrastriatal expression of dopamine-synthesizing enzymes [tyrosine hydroxylase (TH) and aromatic L-amino acid decarboxylase (AADC)]. The most appropriate gene-delivery vehicles for neurons are adeno-associated virus (AAV) vectors, which are derived from non-pathogenic virus. Therefore, TH and AADC genes were introduced into the striatum in the lesioned side using separate AAV vectors in parkinsonian rats, and the coexpression of TH and AADC resulted in better behavioral recovery compared with TH alone. Another strategy for gene therapy of PD is the protection of dopaminergic neurons in the substantia nigra using an AAV vector containing a glial cell line-derived neurotrophic factor (GDNF) gene. Combination of dopamine-supplement gene therapy and GDNF gene therapy would be a logical approach to the treatment of PD.     

糖网Ⅰ号治疗糖尿病视网膜病变临床观察

目的:观察糖网Ⅰ号对糖尿病视网膜病变(DR)的疗效。方法:将52例糖尿病视网膜病变患者(95只眼)随机分为对照组和治疗组。每组又分为单纯型和增殖型。除常规治疗外,治疗组服糖网Ⅰ号,对照组服导生明。观察治疗前、后视力、眼底、血糖及血液流变学变化。结果:治疗组的总有效率为72.5%,显著高于对照组(36.4%),两组疗效比较具有显著性差异(P<0.01)。治疗组疗效优于对照组,单纯型优于增殖型。结论:糖网Ⅰ号治疗糖尿病视网膜病变有显著疗效,尤其是单纯型。