COA: Bone Morphogenetic Protein 2 Promoted Transforming Growth Factor β3 Induced Chondrogenesis of Human Osteoarthritic Synovium-Derived Stem Cells

Background Synovium-derived stem cells (SDSCs) with greater chondrogenic potential are attracting more considerable attention as a cell source for cartilage regeneration. The aim of this study was to investigate the effect of bone morphogenetic protein-2 (BMP-2) on transforming growth factor-beta3 (TGF-β3)-induced chondrogenesis of SDSCs isolated from human osteoarthritic synovium in a pellet culture system. Methods Nucleated cells isolated from human osteoarthritic synovium were plated at an optimal cell density to allow the selective proliferation of SDSCs. The clonogenicity, stem cell marker expression and multi-differentiation potential were determined by CFU assay, flow cytometry assay and specific staining including alizarin red S staining, Oil red staining and alcian blue staining, respectively. SDSCs pellet was cultured in chondrogenic medium without or with TGF-β3 or/and BMP-2. At day 21, the diameter and the weight of the pellets were measured. Chondrogenic differentiation of SDSCs was evaluated by Safranin O staining, immunohistochemical staining of collagen type II, sulfated glycosaminoglycan (sGAG) synthesis and mRNA expression of collagen type II (COL2A1), aggrecan (ACAN), SOX9, link-protein (HAPLN1), collagen type X (COL10A1) and BMP receptor II (BMPR-II). Results Cells isolated under the optimized culturing density (104/60cm2) showed clonogenicity and multi-differentiation potential. These cells were positive (>99% positive) for CD44, CD90, CD105 and negative (<10% positive) for CD34 and CD71. SDSCs differentiated to a chondrocytic phenotype in chondrogenic medium containing TGF-β3 with or without BMP-2. Metachromatic staining of the extracellular matrix with Safarnin O was positive and the expression of collagen type II was detected. The combination of TGF-β3 and BMP-2 produced cell pellets with larger diameter and weight, produced more sGAGs, expression higher levels of collagen type II and chondrogenic markers, except COL10A1, than medium with TGF-β3 alone. Conclusions SDSCs could be isolated from human osteoarthritic synovium. Supplementation of BMP-2 significantly promoted the in vitro TGF-β3-induced chondrogenic differentiation of SDSCs.     

蒲地蓝消炎胶囊治疗小儿急性肠系膜淋巴结炎疗效观察

目的观察蒲地蓝消炎胶囊治疗小儿急性肠系膜淋巴结炎的临床疗效。方法将100例确诊为小儿急性肠系膜淋巴结炎的患儿随机分为两组：对照组50例，予头孢哌酮钠50mg／（kg·d），分2次静脉滴注，利巴韦林10mg／（kg·d），1次静脉滴注，发热或腹痛及呕吐症状严重者予以退热、解痉等对症治疗；实验组在对照组基础上口服蒲地蓝消炎胶囊，3～6岁服用2粒，3岁以下服用1粒，3次/d。结果实验组与对照组的总有效率分别为96％和84％，差异具有统计学意义（P〈0．05）；实验组腹痛消失时间和肿大淋巴结消失时间比对照组短，差异具有统计学意义（P〈0．05）。结论蒲地蓝消炎胶囊治疗小儿急性肠系膜淋巴结炎疗效确切，服用方便，价格便宜，值得临床推广。     

跑步应克服哪些“误区”

<正>对军人来说,跑步不仅是一项健身运动,更是几乎每天必须坚持的体能训练课目,熟悉程度不言而喻。熟悉的事物并不一定真明白。假如有人问你:战友,你会跑步吗?你还真不一定会。担任多年全军软组织伤病康复中心主任张国龙在临床一线和下部队巡诊的过程中,发现了许多因不会科学跑步导致的不适和伤病,不仅损害了个人身体健     

肝癌年龄别发病(死亡)率曲线数学模型拟合的研究

目的：研究对肝癌发病死亡率实际数据的数学拟合模型，有助于指导肝癌预防实践工作。方法：选择香港、上海、天津等8个点的肝癌年龄别发病（死亡）率资料，采用改进的模型对这8处资料进行数学公式模拟，给出了各参数的值，拟合相关系数在0．72～0．99之间，拟合度在0．51～0．98之间。结果：发现年龄别发病（死亡）率曲线的极值即众数Ratemax值降低，则发病（死亡）率曲线高度下降；a值减小，则发病（死亡）率在小年龄时上升，反之则发病（死亡）率曲线右移至大年龄时上升；b值下降发病（死亡）率曲线左移往小年龄；c值下降发病（死亡）率曲线基点升高。结论：肝病预防要抓小，抓早。     

腰椎间盘突出症中神经根炎治疗的重要性：（附132列临床观察）

腰椎间突出症绝大部分病例可经一般的保守疗法获得缓解，其中一部分病例，经过３个月至半年的治疗疗效不佳，本组１３２组，经治疗神经根炎后症状明显缓解而无需手术，证明有效的神经根炎治疗方法有重要的临床意义。     

体育运动与骨骼——骨密度和结构与生物力学适应性

人体运动系统包括肌肉、骨与关节三大器官,是研究人体运动系统中力、杠杆与支点的中心内容.三者的结构与功能的配合最早源于胚胎.出生后,骨骼由于对外界环境尤其是对力学刺激的适应而不断进行塑型(modeling)和改建(remodeling).骨量(bone mass)除受遗传、年龄与性别的影响外,亦受机械力学负荷、激素和营养等因素的调节.人体需保证基本的运动来维持骨量,废用或不负重则导致骨量显著下降.本文主要概述力学刺激(应力)或体育运动对骨骼--骨密度和结构产生适应性改变与调节的机制,并简介相关研究评定指标的现代测量技术与方法.     

聚丙烯单纤丝修补网疝修补50例体会

目的观察聚丙烯单纤丝修补网修复腹壁疝的临床效果。方法对采用聚丙烯单纤丝修补网治疗50例腹壁疝患者的临床效果进行分析。结果单侧手术时间30-60min，平均（40.5±10.2）min，术后疼痛轻，可早期下床活动和进食，无血肿，无术后感染，无复发。结论聚丙烯单纤丝修补网适合修复各型腹壁疝，并发症少，恢复快。     

农村卫技人员配置量影响因素分析与建模

文章依据1993年国家卫生服务总调查65县资料，对专家咨询选出的18项社会经济指标作多元逐步回归和因子分析，筛选出农业人口比重、5岁以下人口比例、年人均门诊次数、年人均住院天数、地形特征、期望寿命、通公路村比例7项指标作为卫技人员配置量的主要影响指标，建立量化模型，经检验，模型拟合效果满意，可作为因地制宜配置农村卫技人员的量化工具。     

骨形态发生蛋白12,13对促进髌腱愈合的体外细胞培养研究

骨型态发生蛋白(bone morphogenetic protein，BMP)是转移生长因子(TGF)-β家族中的成员之一，在胚胎形成和组织修复过程中对骨和软骨的形成起诱导作用^[1,2]。BMP12和BMP13在肌腱损伤修复过程中所起的作用机制尚未有研究。本实验应用人髌腱分离培养的成纤维细胞进行体外相关实验，评估BMP12和BMP13可能促进肌腱损伤愈合的作用。