胃动素对血管灌流大鼠离体胃运动的作用

通过血管灌流大鼠离体胃,探讨胃动素对胃运动的影响。结果表明:(1)胃动素可以明显地兴奋胃窦自发的胃运动;(2)胃动素抗血清可以完全消除胃动素兴奋胃窦运动的作用;(3)阿托品可以阻断胃动素兴奋胃窦运动的作用。上述结果提示,胃动素可特异性兴奋血管灌流大鼠胃窦收缩运动,该作用通过壁内胆碱能神经介导。     

Annexin A2的结构和功能及其与疾病的关系

Annexins是一类钙依赖性膜磷脂结合蛋白。Annexin A2是Annexins家族中的一个重要成员,存在于细胞膜、胞质和胞外。Annexin A2蛋白在细胞膜构架的形成、膜聚合、内吞和胞吐中均扮演重要角色。Annexin A2还参与某些病理过程,与心血管疾病、血液病、肿瘤等多种疾病相关。文章简要综述了Annexin A2分子的结构、生化特征、生物学功能及其与疾病的关系。     

21-三体嵌合型母亲二次妊娠21-三体患儿

目的报告1例嵌合型母亲连续2次孕育单纯型21-三体患儿。方法对21-三体患儿、21-三体胎儿和其父母的血标本、父亲精子进行了DNA多态性检测,选择2个SRTR多态性位点,即D21S1412和D21S11,进行荧光定量PCR检测以确定额外21号染色体的起源。对父亲和母亲的外周血染色体和母亲皮肤染色体也进行了分析。结果对2个患儿与双亲的STR结果进行比较,显示2个患儿的额外21号染色体都来自母亲。双亲的STR检测中仅发现有2个微卫星标志物,外周血淋巴细胞染色体也均为正常。但母亲皮肤成纤维细胞检测到21-三体的细胞存在,占4%。结论母亲是21三体细胞/正常细胞的嵌合体,推测母亲生殖细胞中存在21三体细胞与正常细胞的嵌合,这可能是导致连续2次孕育21-三体患儿的原因。     

遗传性果糖不耐受症

遗传性果糖不耐受症（HFI）是一种罕见的、由于先天性醛缩酶B缺陷导致的果糖代谢病。为常染色体隐性遗传性疾病。特征是摄取果糖、蔗糖或山梨醇后发生严重的低血糖。若不及时终止此类食物，会导致肝肾功能损伤及生长发育障碍。本病诊断比较困难，治疗主要以对症治疗和饮食控制为主。本文就遗传性果糖不耐受症的临床生化特征、诊治方法及醛缩酶B损伤的分子学基础进行综述，为临床早期发现、早期诊断、早期治疗遗传性果糖不耐受症提供参考。     

人和大鼠腰椎关节突关节的SP能神经纤维的分布

目的：证实支配腰椎关节突关节的神经支配和化学性质，方法：用逆行荧光素标记结合免疫组化法，研究７只大鼠腰部脊神经节细胞的周围突分支投射到腰椎关节突关节及其递质性质以及３例人腰椎关节突关节囊上神经末梢的化学性质，结果：发现大鼠一侧Ｌ５和Ｌ６之间的关节突关节受同侧Ｌ２－５节段的脊神经节的部分细胞周围突分支支配，其中有３３．３９９％的中型和小型细胞为中ＳＰ能免疫反应阳性，人的关节突关节囊含有ＳＰ阳性的神经     

Retrospective analysis of non-A-E hepatitis: possible role of hepatitis B and C virus infection

In an effort to determine the cause of non-A-E hepatitis, a retrospective study was undertaken on a group of patients with hepatitis but without serological infection markers of hepatitis viruses A-E. A total of 60 patients admitted to Beijing Ditan Hospital during the period of September 1997 and September 1999 were chosen for this study. These patients were diagnosed as either acute or chronic hepatitis, but no serological markers of hepatitis viruses A-E were detected. Since TT virus (TTV), human parvovirus B19 (B19), SEN virus (SENV), and GB virus C/HGV were reported to be associated with hepatitis, attempts were made to detect the presence of these viruses in the sera of patients with non-A-E hepatitis by a nested polymerase chain reaction (nPCR) method. Also, more sensitive nPCR and RT-nPCR methods were used to determine HBV DNA and HCV RNA in these patients. Results derived from these analyses demonstrate that HBV DNA was detected in most of these patients (47/60, 78.3%), suggesting that HBV infection played a major role in occult non-A-E hepatitis and detection of HBV DNA by more sensitive PCR methods such as nPCR should be considered for diagnosis of HBV infection. In addition, HCV RNA was detected in three (5%) of these patients. However, GBV-C (HGV) RNA was not detected, and TTV, B19, and SENV appear not to be associated with non-A-E hepatitis, as the prevalence rates of these viruses in patients with non-A-E hepatitis were similar to those in patients with viral hepatitis A-E. The results from this study indicate that co-infection of TTV or B19 with HBV did not increase the severity of the disease.     

阿昌族与汉族维生素D受体基因Fok多态性

目的　了解维生素 D受体基因多态性在中国不同民族中的分布。方法　应用聚合酶链反应 -限制性片段长度多态性分析、基因测序等技术检测 6 8名阿昌族人和 92名汉族人的维生素 D受体基因Fok 多态性 ,比较两组维生素 D受体基因型和等位基因的分布频率。结果　在 6 8名阿昌族人中 FF基因型占 18%、Ff基因型占 35 %、ff基因型占 4 7% ,而在 92名汉族人中 FF基因型占 2 2 %、Ff基因型占 5 2 %、ff基因型占 2 6 %。两组维生素 D受体基因型的分布频率差异有显著性 (χ2 =7.716 ,P=0 .0 2 1)。结论　阿昌族与汉族维生素 D受体基因 Fok 多态性分布频率差异有显著性。     

白血病和正常血细胞的一些细胞因子及受体表达的比较研究

目的:研究白血病细胞和正常血细胞的细胞因子及相应受体(R)的表达及其意义。方法:采用逆转录-聚合酶链反应方法检测红白血病细胞系(HEL、K562)、髓系白血病细胞系(HL-60)、单核细胞白血病细胞系(U937)、巨核细胞白血病细胞系(DAMI、MEG-01)、T淋巴瘤细胞系(HUT78)及EB病毒感染的B细胞系(CA)和正常血细胞(CD34⁺造血干细胞、外周血单个核细胞、成熟粒细胞、巨核细胞及血小板)的多种细胞因子及受体的表达。结果:①CD34⁺造血干细胞同时表达IL-1(α、β)、IL-3、IL-6、GM-CSF、G-CSF及相应受体和干细胞因子受体(SCFR)、血小板生成素受体(MPL)基因,在成熟粒细胞仅表达IL-6、IL-6R、G-CSFR、GM-CSF,巨核细胞、血小板仅表达IL-3R、IL-6、IL-6R、MPL。而TGFβ₁、TNFα及相应受体在上述正常血细胞均有持续表达。②HEL、K562、HL-60、U937、DAMI、MEG-01、HUT78及CA可同时表达至少两种以上正调控因子及相应受体,而负调控因子TGFβ₁、TNFα及受体在上述各细胞系均有表达。结论:白血病细胞株细胞存在正性多自泌环节,白血病细胞的正、负自分泌因子失衡与白血病发病有重要关系。     

“Smart” Materials for Biosensing Devices: Cell-Mimicking Supramolecular Assemblies and Colorimetric Detection of Pathogenic Agents

Mimicking cell membrane and the biomolecular recognition associated with membranes represents a great technical challenge, yet it has opened doors to innovative diagnostic and therapeutic methods. Our work has focused on design and synthesis of a class of smart materials exploiting biological principals for use in biosensors: these materials are functional polymeric assemblies that mimic the cell membrane and conveniently report the presence of pathogens with a color change. Biologically active cell membrane components are incorporated into conjugated polymers with desirable optical properties and the binding of the target molecules onto the material triggers conformational and electronic shifts that are reflected in a chromatic change (a so-called biochromic shift) that is conveniently observed and recorded. Langmuir–Blodgett thin films and vesicle bilayers provide ideal configurations for precise delivery of the biological binding entity to the sensing interface, and for control of molecular orientation for effective biomolecular interaction. Polydiacetylenic membrane-mimicking materials containing cell surface receptor gangliosides and sialic acid residues, respectively were formulated into these architectures and used for colorimetric detection of bacterial toxins and influenza virus. One advantage of these biochromic conjugated polymer (BCP) sensors is that their molecular recognition and signal transduction functionalities are resident in a single functional unit, making them amenable to convenient microfabrication and use.     

产前血型IgG抗体水平的检测及其临床意义

目的探讨ABO血型不合孕妇的产前IgG抗体水平,了解IgG抗体效价异常在孕妇中所占比率及临床意义,为预防及诊治新生儿溶血病（HDN）采取有效的防治措施。方法用抗人球蛋白试管凝集法进行IgG抗A或抗B的ABO血型抗体效价检测。结果910例孕妇中,血清效价大于64者有108例,异常检出率为11.9%。IgG抗A效价大于64者有64例,检测率为11.8%;检测IgG抗B效价大于64者有54例,检测率为14.1%。讨论妊娠中IgG抗体效价与新生儿溶血密切相关,ABO血型不合的孕妇应及时作产前血清学的检测,可预防新生儿溶血病的发生及减轻胎儿受害的程度。