Complement Killing of Yersinia enterocolitica and Retention of the Bacteria by Leucocyte Removal Filters

We report studies on the complement sensitivity of four strains of Yersinia enterocolitica , serotypes O:3, O:9, O:5,27, and O:20, isolated from blood units involved in transfusion fatalities. Complement in fresh CPD plasma killed Y. enterocolitica within 4 h at 22°C in 100% of the experiments. The bactericidal action was serotype and complement activation pathway dependent. Both classic and alternate pathways seemed to be active, but the latter to a lesser degree. When the classic pathway was blocked by chelation of Ca²⁺ no complete killing was obtained. Complement did not enhance or condition Yersinia for leucocyte filter retention. Direct removal of Yersinia by filtration was also related to serotype; all strains were reduced by filtration in heat-inactivated plasma, and all except serotype O:5,27 were reduced in Ca²⁺ -chelated plasma. Our findings may explain why plasma products and platelet concentrates are rarely involved in Yersinia sepsis related to transfusion.     

Man-Machine Interfaces in Computer Assisted Surgery

The clinical potential of computer assisted surgery (W) has been more and more widely acknowledged since CAS systems have been introduced into the operating room (OR) theater.

Especially the improvements in safety and accuracy are remarkable and strengthen the ties between surgeons and engineers. Tumor stereotaxis was introduced to neurological surgery in the early 1980s, and currently systems with and without robotic navigation are in use for specific medical indications. Recently, solutions for computer assisted orthopedic surgery were developed and applied to various anatomical regions. However, with the establishment of CAS in vivo, a new complex of problems, which was not present in the laboratory setup, was introduced: the man-machine interface.

Currently, the complexity of available CAS systems requires the presence of at least one system engineer (often called the “operator”) in the OR. As a consequence, there is no possibility for direct communication between the surgeon and the machine or software.

Most of the program steps involved in CAS and choices to be made intraoperatively have to be transferred to the software by means of communication of the surgeon with the operator. Particularly, the establishment of a relation between the virtual object (i.e., a medical image) and the surgical object (i.e., the patient), often denoted as “matching” or “skeletal registration” requires intensive interaction of the surgeon with the computer. A literature survey revealed that no CAS system in clinical use exists without a system engineer or a comparable person, and our clinical experience indicated that the matching process is a weak point in most systems. Because it appears to be contradictory to cost-reduction efforts in health care to have a highly paid specialist in the OR, this research evaluates strategies to facilitate the man-machine interface with the final goal of establishing a direct control of the system by the surgeon or the medical personnel traditionally present at surgery. Options to be investigated include 1) a CAS control panel (virtual keyboard) as an integrated component of the existing navigation system and 2) introduction of a commercial voice-recognition system. The implementation of these strategies into the existing CAS setup at the Department of Orthopaedic Surgery at the Inselspital (University of Bern) and clinical experience gained are reported     

有机氮源对红霉素发酵影响的具体分析

红色糖多孢菌的摇瓶培养基中使用不同有机氮源时红霉素的效价明显不同。通过对不同有机氮源所含营养成分的逐步回归分析,我们得到有机氮源中的苏氨酸为影响红霉素效价的主要因子。最后向对照培养基中添加苏氨酸,则使红霉素的效价比对照培养基提高了22.85%。     

肺癌患者血清肿瘤特异生长因子检测及临床意义

目的 :研究TSGF在肺癌诊断、疗效判定中的临床应用价值。方法 :利用生化比色法测定了 18例肺良性病变患者 ,2 4例肺癌患者及 9例肺癌患者治疗后血清中TSGF水平。结果 :肺癌患者未经治疗前其血清TS GF水平及阳性率明显高于肺良性病变患者 ,其敏感性为 75 .0 % ,特异性为 77.8%。经有效化学治疗后肺癌患者血清TSGF明显下降。结论 :血清TSGF检测对肺癌的诊断、疗效观察有较重要的意义。     

安定对大鼠局灶性脑梗死半影区神经细胞死亡的保护作用

目的　研究安定对光化学诱发脑梗死后细胞死亡的保护作用 .方法　使用光化学诱发的大鼠局灶性脑缺血模型 .采用原位末端标记技术 (TU NEL)检测凋亡细胞 .大鼠脑梗死术后存活 3,6 ,12 ,2 4 ,4 8和 72 h,分别观察梗死灶坏死中心面积和凋亡细胞数目 .术前 2 4 h开始腹腔注射安定 (10mg·kg- 1· 8h- 1 ) ,直到处死动物 .结果　TUNEL 阳性细胞位于坏死中心与正常皮层之间的半影区 .TUNEL 阳性细胞的均数随着梗死后时间的延长逐渐增多 ,2 4 h达到高峰 .在2 4 h时间点 ,安定治疗组梗死坏死中心的平均最大面积和TUNEL阳性细胞均数与对照组相比 ,明显减少 (P<0 .0 0 1) .结论　安定可明显减少成年大鼠局灶性脑梗死后的细胞坏死和凋亡 .该结果可为临床使用安定治疗缺血性脑血管病提供理论依据     

喷发胶所致酒精中毒

一位有酒精依赖病史的61岁老年女性因酒精中毒被送到急诊科，其血浆酒精浓度为322mg／dl。她承认因为发胶中非自然的的酒精成分，她混水应用了一瓶14盎司的发胶。最初她在参加一次酒精匿名会议时学会了这种应用非饮料酒精的方法，为了向家人隐瞒滥用酒精的事实，她开始应用并持续了数年。她应用的发胶的非自然酒精含量高达50％．其吸收量约为7盎司酒精，相当于141．25盎司80标准强度的酒精饮料。     

青阳参甙元的分子结构研究

青阳参甙是一种已用于临床,对癫痫病有较好疗效的药物,其二乙酰甙元的立体分子结构用x射线衍射方法进行了测定,并与几种作用于神经系统药物的分子结构作了对比,还讨论了它们共同具有的相似的活性基团。