717离子交换树脂对L-组氨酸的吸附及应用

目的确定717树脂从碱性氨基酸粗提液中分离L 组氨酸的工艺条件。方法采用静态吸附实验。测定吸附动力学曲线确定吸附达到平衡时间;测定吸附等温线确定吸附的适宜温度和最大吸附量;测定不同pH条件下的吸附量确定吸附的适宜pH值;测定Cl-浓度对吸附量的影响,分析阴离子的存在对吸附的影响。结果30min可达到吸附平衡;吸附可在常温下进行,最大吸附量约为85 g·kg-1;吸附的适宜pH值约为8 0～9 0 ;氯离子对吸附的影响很大。结论采用717离子交换树脂可以将L 组氨酸与L 赖氨酸的交叉洗脱液中的L 组氨酸分离出来     

PLC对ADP诱导的兔血小板cAMP的影响

目的　测定磷酯酶C(phospholipaseC,简称PLC,下同)对ADP诱导的兔血小板cAMP的影响,从而进一步阐明PLC抗血小板聚集作用的机制。方法　取家兔的PRP,除去红细胞,分别用生理盐水、ASA和不同剂量的PLC处理,以ADP诱导激活,采用三氯醋酸法制备血小板cAMP,再用放射免疫分析法分别测定cAMP含量。结果　家兔血小板经生理盐水处理后的cAMP含量为 ( 20 16±0 91 ) pmol/109platelets,而经生理盐水、ASA668μmol·L-1、5、10、15、20和25UPLC·ml-1各组处理,并以ADP激活的血小板,测得的cAMP含量分别为 13 85±1 14, 16 27±0 36, 18 74±0 55,19 80±0 52, 20 49±0 43, 22 55±0 61和 24 24±0 85(pmol/109 platelets)。以生理盐水作为对照,ASA668μmol·L-1、5、10、15、20和 25UPLC·ml-1剂量组对激活状态下血小板cAMP的含量有增加的作用,增加率 (% )分别为17 47, 35 31, 42 96, 47 94, 62 82, 75 02。结论　PLC使得ADP激活后的兔血小板cAMP水平提高(P<0 01),且呈剂量依赖性,表明PLC具有提高血小板中cAMP水平的作用。这可能是PLC抗血小板聚集作用的重要原因之一。     

病位证素的特征

对脑[神]、心、肺、脾、肝、肾、胃、胆、小肠、大肠、膀胱、胞宫[精室]、胸膈[上焦]、少腹[下焦]、表、半表、半里、经络、肌肤[皮肤、肌肉]、筋骨[关节]等19项病位证素的概念，各证素的临床表现及其证候属性，与相关证素间的联系规律，常见证型等进行了规范性论述。     

八宝眼粉质量标准的研究

目的:建立八宝眼粉的质量控制方法.方法:采用化学反应和薄层色谱法(TLC)鉴别方中炉甘石、人工牛黄、冰片;采用气相色谱法(GC)测定冰片的含量.结果:鉴别效果满意;冰片在0.09808～0.78464 μg范围内呈良好的线性关系,平均加样回收率为101.5%,RSD=1.40%(n=5).结论:本方法简便、快速准确,可作为该制剂质量控制方法.     

杞蓉药酒质量标准的研究

目的:建立和完善杞蓉药酒(当归、补骨脂、制何首乌、枸杞子、红花等)质量标准.方法:采用TLC法鉴别了当归、补骨脂、制何首乌、枸杞子、红花5味药,采用HPLC法测定了补骨脂素和异补骨脂素的含量.结果:补骨脂素平均加样回收率为98.7%,RSD为2.18%,n=5;异补骨脂素平均加样回收率为100.8%,RSD=1.67%,n=5.结论:本方法简便、快速准确,可作为该制剂的质量标准.     

A New Prenylated Xanthone from Root Barks of Cudrania cochinchinensis

Objective To study the chemical constituents from the root barks of Cudrania cochinchinensis. Methods The chemical constituents were isolated and purified by silica gel column chromatography. The structures of the compounds were identified on the basis of spectral data (MS, 1H-NMR, 13C-NMR, and 2D NMR) and by the comparison of spectroscopic data with the reported values in the literatures. Results A new xanthone, 1,6,7-trihydroxy-4-(1,1- dimethylallyl)-3-methoxyxanthone (1) and a known prenylated xanthone 1,5,6-trihydroxy-4-(1,1-dimethylallyl)-3- methoxyxanthone (isocudraniaxanthone B, 2) were isolated from the root barks of C. cochinchinensis. Conclusion Compound 1 is a new prenylated xanthone. Isomers 1 and 2 are obtained from this plant for the first time.     

One‐Year Clinical Outcome of a Randomized Trial of Polymer‐Free Paclitaxel‐Eluting Stents Versus Biodegradable Polymer‐Based Rapamycin‐Eluting Stents in Patients with Coronary Heart Disease

Background: More widespread use of drug‐eluting stents (DES) to treat coronary heart disease (CHD) has recently generated more attention to thrombosis, which was relative to the polymer. Polymer‐free and biodegradable polymer‐based stents are more frequently studied, but their efficacy on preventing detrimental clinical events is unclear. Methods and Results: To assess whether polymer‐free paclitaxel‐eluting stent (YINYI stent) was noninferior or equivalent to biodegradable polymer‐based rapamycin‐eluting stents (EXCEL stent) in preventing detrimental clinical cardiovascular events, a total of 167 consecutive CHD patients requiring DES implantation were randomly divided into the YINYI group (n = 82) and the EXCEL group (n = 85). The primary end‐point was major adverse cardiac events (MACE). The secondary end‐points included stent thrombosis events, all‐cause mortality, and rehospitalization. The study was designed to test the noninferiority or equivalence of the YINYI stent compared with the EXCEL stent with respect to one‐year MACE according to a noninferiority or equivalence margin of 0.1. One‐year MACE was 6.10% in the YINYI group versus 5.88% in the EXCEL group. The lower limit of the one‐sided 95% confidence interval was ?0.0582 (P = 0.002 from the test for noninferiority). The 95% confidence interval for the equivalence test was [?0.0698, 0.0742] (P₁=0.004 and P₂=0.007 from 2 times the 1‐sided test for equivalence). There was no statistically significant difference in thrombosis events, all‐cause death, and rehospitalization (all P > 0.05). Conclusions: In this small randomized trial, polymer‐free paclitaxel‐eluting stents appear to be noninferior or equivalent to biodegradable polymer‐based rapamycin‐eluting stents. (J Interven Cardiol 2012;25:604–610)     

自体血液回收对全髋关节置换术患者炎性因子的影响

目的:观察自体血液回收对骨科手术患者炎性因子的影响。方法:40例择期行全髋关节置换手术患者,ASA分级Ⅱ级,采用随机数字表法分为两组:自体血液回收组(A组)和异体输血组(B组),每组20例。回收组术中应用血细胞回输仪进行血液收集、回输,两组均于输血前(T1)、输血后1h(T2)、4h(T3)、8h(T4)四个时点测定血浆TNF-α、IL-6、IL-10浓度。记录患者术中回收血量,异体浓缩红细胞量及血浆用量。结果:手术开始至术后24h内回收组输注异体浓缩红细胞量及血浆用量较对照组明显减(P<0.05);两组患者输血后TNF-α、IL-6、IL-10较输血前显著均增高(P<0.05),与A组比较,B组TNF-α、IL-6在输血后各时点显著增高,而IL-10则明显降低(P<0.05)。结论:全髋置换术中使用自体血回收技术,可明显减轻的TNF-α、IL-6、IL-10等炎性因子表达程度的改变。     

探讨医院社区联合康复模式对精神分裂症患者社会功能的影响

目的观察医院社区联合康复模式对精神分裂症患者社会功能的影响。方法将2015年1月至2018年12月在本社区登记在案的80例精神分裂症患者,根据登记时间顺序分两组,每组40例。对照组采取常规社区精神卫生服务,联合组采取医院社区联合康复模式。观察比较两组管理前后社会功能(SDSS)、精神分裂症阳性与阴性症状量表(PANSS),复发率、再住院率、再就业率。结果两组患者管理后SDSS、PANSS评分较管理前明显提高,且联合组各项评分明显高于对照组,管理组与对照组患者复发率(2.50%vs.22.50%),再住院率(2.50%vs.20.00%),再就业率(82.50%vs.47.50%),差异具有统计学意义(P<0.05)。结论在精神分裂症患者的康复中,采取医院社区联合康复模式,可有效减少其精神症状,改善患者社会功能,减少复发率与再住院率,明显提高患者再就业率。