腺苷对急性出血坏死性胰腺炎肠道屏障功能保护机制的实验研究

目的研究腺苷对猪急性出血坏死性胰腺炎(AHNP)肠道屏障功能保护作用的机制.方法12只成年小型猪经ANHP成模后随机均分成对照组和腺苷治疗组(简称腺苷组),比较两组动物在AHNP诱发前后不同时相点肠道血流量、肠道通透性、门静脉血细菌和内毒素的变化.结果(1)腺苷组在AHNP成模后8,24h和第7d时肠道血流量显著高于对照组(P<0.01或P<0.05);(2)腺苷组成模后8,24h和第3d肠道通透性显著低于对照组(P<0.05或P<0.01);(3)对照组门静脉血细菌和内毒素明显增加,腺苷组门静脉血细菌数在AHNP诱导后第24h和第3d较对照组显著下降(P<0.05或P<0.01);门静脉血内毒素含量显著低于对照组各时相点(P<0.01或P<0.001).结论腺苷能显著增加肠道血流量;腺苷可以明显减轻肠粘膜损害,保护肠道屏障功能,腺苷的这种作用可能是通过增加肠道血流量而实现的;腺苷可以明显减少肠道细菌和内毒素移位.     

脊柱后方结构在腰椎退变疾患中的作用

目的 研究脊椎后方结构在腰椎退变疾患中的作用。方法 33例退行性腰椎滑脱（DS）,36例腰椎峡部不连滑脱（IS）,30例腰椎间盘突出（LDH）,采用X线片测量椎间盘高度比,CT片测量小关节角和小关节横径,并作组间比较。结果 与IS和LDH组相比,DS组小关节角偏矢壮位,关节横径和椎间盘高度比减少。IS参数与LDH相似。结论 小关节形态在腰椎退行性滑脱机制中起重要作用。     

门静脉高压症外科手术后门静脉系统血栓形成的原因及防治

目的　探讨门静脉高压症外科手术后门静脉系统血栓形成的原因及处理。方法　回顾性分析我院 1992～ 2 0 0 1年施行肝炎后肝硬化门静脉高压症手术伴脾切除术 32 9例患者的临床资料 ,对其中 4 3例 (13 1% )术后出现门静脉系统血栓患者的临床资料进行统计和分析。结果　 4 3例门静脉系统血栓患者中 ,1例发生感染性门静脉炎死亡 ,其余均康复出院。单纯行脾切除或脾切除加断流术患者 138例 ,血栓形成 2 6例 (18 8% ) ;行脾切除加分流术患者 191例 ,血栓形成 17例 (8 9% ) ,差异有显著意义 (χ2 =8 4 4 ,P <0 0 1)。结论　门静脉高压症外科手术后门静脉系统血栓形成的主要原因是脾切除术后血小板升高 ,且与选择不同术式导致术后门静脉系统血流动力学改变有关。手术操作规范化、术后动态监测血小板总数、常规彩超检查及早期行抗凝祛聚疗法是防治门静脉高压症术后门静脉系统血栓形成的有效方法。     

巨大肝癌的外科治疗：附146例报告

目的　探讨巨大肝癌外科治疗的效果。方法　回顾性分析近 11年间收治的 14 6例原发性巨大肝癌的外科治疗情况。 14 6例均经手术治疗 ,其中行不规则肝切除 99例 ,半肝切除 2 8例 ,右三叶切除 4例 ,包括术前行TACE后获得二期切除 19例 ;6例同时行门静脉切开取栓术。另 15例仅行活检 DDS ,术中行DDS治疗者 10 5例。结果　手术并发症发生率 10 .3 % ,术后 1个月内病死率1.4% ,术后 1,3 ,5年和 10年的生存率分别为 5 6.6% ,3 0 .2 % ,14 .4%和 2 .1%。结论　巨大肝癌仍以手术切除为主 ,疗效满意。结合TACE、门静脉取栓和DDS能提高患者的远期生存率。     

Changes of interleukin-1 β, tumor necrosis factor α and interleukin-6 in brain and plasma after brain injury in rats

Objective: To study the changes of interleukin-1 β(IL-1β), tumor necrosis factor α (TNFα) and interleukin-6 (IL-6) levels in brain and plasma after brain injury and to assess the relationship between the cytokine levels and injury severity in rats. Methods. A total of 51 male Wistar rats, weighing 280-340g, were anesthetized with chloral hydrate(400 mg/kg body weight) through intraperitoneal injection and fixed on a stereotaxic instrument. Severe brain injury was created in 16 rats (severe injury group) and moderate brain injury in 18 rats (moderate injury group) by a fluid percussion model, and cytokine levels of IL-1β, TNFα and IL-6 were measured with biological assay. And sham operation was made on the other 17 rats (control group). Results: In the control group, the levels of IL-1β,TNFα and IL-6 were hardly detected in the cortex of the rats, but in the ipsilateral cortex of the rats in both injury groups, they increased obviously at 8 hours after injury.The increasing degree of these cytokines had no significant difference between the two injury groups. The levels of IL-6 in the plasma of all the rats increased slightly, whereas the levels of IL-1β and TNFα were undetectable. Conc|usions: The increase of IL-1β, TNFα and IL-6 levels is closely related to brain injury. The increased cytokine levels in the central nervous system are not parallel to those in the peripheral blood. It suggests that inflammatory cytokines play important roles in the secondary neural damage after brain injury.     

尿毒症患者血液透析致残余肾功能减退相关因素分析

目的：分析血透致残余肾功能(RRF)减退的相关因素。方法：采用醋酸纤维素膜透析器(1.1及1.7 m2)行碳酸氢盐透析,并每月测定1次24 h尿量、肌酸酐水平、透析间期体质量增长量、透析清除率及透析中反应,共观察6-12个月。结果：原发于肾小球疾病者RRF较肾小管间质疾病者下降快;长期透析超滤超过4.0 kg/次者较小于4.0 kg/次者RRF下降快;透析6个月RRF减退幅度超过5 ml/min者透析并发症发生率与低于5 ml/min者相比差异不显著;采用大面积(1.7 m2)透析器治疗6个月后RRF下降幅度较采用中等面积透析器(1.1 m2)大。结论：大量超滤引起肾有效血流量降低,致肾缺血再灌注损伤,是造成血透初期残余肾功能丢失的主要因素;追求高效透析致渗透性利尿作用减弱,也是加速RRF减退的原因;肾小球疾病,尤其病变处于活动阶段,参与了RRF的丧失。     

牡蛎壳与龙骨成分的分析

目的　通过对牡蛎壳与龙骨成分分析,为牡蛎壳替代龙骨提供药用价值方面的依据。　方法　采用原子吸收光谱法、等离子发射光谱法和氨基酸自动分析法对闽产牡蛎壳与龙骨成分进行分析。　结果　牡蛎壳和龙骨的钙含量分别为３９．７８％ ±０．２３％ , ３１．９８％ ±０．１４％ ;两者均含有９ 种微量元素;龙骨含７ 种氨基酸,牡蛎含１７ 种氨基酸。　结论　牡蛎壳与龙骨的无机元素成分基本相同。     

精神分裂症与ABO血型等相关因素的分析

目的探讨精神分裂症与ABO血型、家族遗传史等致病因素之间的关系。方法用临床常规的正反定型法对病人ABO血型检测，并与正常人血型对照。同时，还对患者家族遗传史、发病诱因进行分析。结果204例精神分裂症患者ABO血型分布与518例正常人比较差异无显著性。患者性别与血型分布比较差异无显著性，但男性患者血型分布为B型〉O型，女性患者血型分布为O型〉13型。结论精神分裂症男性患者中B型比例较高，女性患者中O型较高。     

克癌7851抗癌作用的实验研究

本文报告了由铜、铁微量元素与清热解毒、活血化瘀中草药组成的克癌7851对小鼠肝癌和肺癌抑制作用。对肝癌抑制率45.9％～73.5％,肺癌34.3％～35.9％。与对照组相比,荷瘤动物活存动态曲线明显抬高,癌细胞分裂指数下降,对照组33.4‰～39.8‰,给药组19.6‰～24.2‰,癌细胞DNA含量较少,对照组42.9～47.2 A.U.,给药组33.5～41.1 A.U.,组方图明显左移。     

Diallyl trisulfide inhibits cell migration and invasion of human melanoma a375 cells via inhibiting integrin/facal adhesion kinase pathway

Melanoma is the leading cause of death from skin disease due to its propensity for metastasis. Studies have shown that integrin‐mediated focal adhesion kinase (FAK) signal pathway is implicated in cell proliferation, survival and metastasis of tumor cells. Our previous results indicated that diallyl trisulfide (DATS) provided its antimelanoma activity via inducing cell cycle arrest and apoptosis. The aim of this study was to explore DATS mediated antimetastatic effect and the corresponding mechanism in human melanoma A375 cells. We found that DATS exhibited an inhibitory effect on the abilities of migration and invasion in A375 cells under noncytotoxic concentrations analyzed by wound healing assays and Matrigel invasion chamber system. DATS attenuated invasion of A375 cells with characteristic of decreased activities and protein expressions of matrix metalloproteinase‐2 (MMP‐2) and MMP‐9. Moreover, DATS exerted an inhibitory effect on cell adhesion of A375 cells, which is in correlation with the change in integrin signaling pathway. Results of Western blotting showed that DATS decreased the levels of several integrin subunits, including α4, α5, αv, β1, β3 and β4. Subsequently, DATS induced a strong decrease in total FAK, phosphorylated FAK Tyr‐397,‐576, ?577, and disorganized F‐actin stress fibers, resulting in a nonmigratory phenotype. These results suggest that the antimetastatic potential of DATS for human melanoma cells might be due to the disruption of integrin/FAK signaling pathway.