Can Sensitized Lymphocytes Retain Reactivity to Inner Ear Antigens After Retrieval From Frozen Storage?

Immune inner ear disease results in rapidly progressive, bilateral sensorineural hearing loss and is one of the few forms of sensorineural hearing loss that can be treated medically. The purpose of this study is to identify and preserve several populations of sensitized lymphocytes from patients with immune inner ear disease as a first step toward cloning autoreactive T cells, in order to study the pathogenesis of disease. Lymphocytes from four patients with high reactivity (stimulation index of 2.5 or greater) were placed in frozen storage. At 8 to 14 months they were thawed and restimulated. All four samples were viable. Two reacted again to inner ear homogenate, but with different intensities. Some lymphocytes sensitized to inner ear antigens can retain reactivity after frozen storage. This methodology may be useful to clone highly reactive T cells.     

结直肠癌伴穿孔的诊治体会

目的：探讨有效地预防结、直肠癌伴穿孔的发生及合理治疗结、直肠癌伴穿孔的方法。方法：用X2检验分析742例患者的5年生存率与其中的11例伴穿孔患者的5年生存率（所有病例均随访5年以上）。结果：全部742例结、直肠癌患者的5年生存率为58.6％。11例伴穿孔患者的5年生存率仅为18.2％。4例伴急性穿孔患者中的3例有诱发穿孔的因素。穿孔的部位大多发生在左侧结肠；其病理类型多数为粘液腺癌。结论:结、直肠癌伴穿孔患者的预后很差，在诊治过程中避免医源性的诱发穿孔因素值得重视；特别是结、直肠癌伴不全性梗阻时，要慎重选择检查手段及清洁肠道准备的方法。     

The relationship between dietary fat intake and risk of colorectal cancer: evidence from the combined analysis of 13 case-control studies

The objective of this study was to examine the effects of the intakeof dietary fat upon colorectal cancer risk in a combined analysis of datafrom 13 case-control studies previously conducted in populations withdiffering colorectal cancer rates and dietary practices. Original datarecords for 5,287 cases of colorectal cancer and 10,470 controls werecombined. Logistic regression analysis was used to estimate odds ratios (OR)for intakes of total energy, total fat and its components, and cholesterol.Positive associations with energy intake were observed for 11 of the 13studies. However, there was little, if any, evidence of anyenergy-independent effect of either total fat with ORs of 1.00, 0.95, 1.01,1.02, and 0.92 for quintiles of residuals of total fat intake (P trend =0.67) or for saturated fat with ORs of 1.00, 1.08, 1.06, 1.21, and 1.06 (Ptrend = 0.39). The analysis suggests that, among these case-control studies,there is no energy-independent association between dietary fat intake andrisk of colorectal cancer. It also suggests that simple substitution of fatby other sources of calories is unlikely to reduce meaningfully the risk ofcolorectal cancer.     

补肾填精方对老龄大鼠脑细胞脂褐素及外周血SOD.LPO含量的影响观察

补肾填精方具有降低老龄鼠脑细胞脂褐素的含量，改善外周血ＳＯＤ、ＬＰＯ浓度的作用，有一定的延缓衰老的作用。     

复方虎茵汤方药配伍对虎杖有效成分煎出的影响

通过复方虎茵汤方药配伍煎提对虎杖有效成分煎出的影响，探讨虎杖与大枣、茵陈的配伍规律。结果显示：虎杖单煎，其有效成分总蒽醌、游离蒽醌、总大黄素、游离大黄素含量最高，而复方煎煮虎杖有效成分的煎出率最低。     

结直肠癌组织微量元素的分析电镜研究

目的：探讨结、直肠癌组织中的微量元素变化。方法；应用分析电镜对37例结、直肠癌，移行粘膜和正常粘膜组织中元素进行研究。结果；在结、直肠癌中磷元素和钾元素相对含量显著高于正常粘膜（P＜0．05）。结论：上述结果提示在结、直肠癌中除微量元素钙、铜、锌和锰含量降低以外，元素磷和钾含量增加。     

正常青春期少女及特发性中枢性性早熟女孩生长激素结合蛋白、性激素与生长

本文对41例青春期女孩及30例特发性中枢性性早熟(ICPP)女孩。观察其身高、体重、身高增长速度、骨龄与生长激素结合蛋白(GHBP)、雌二醇(E_2)的相关关系,结果示GHBP在青春各期间无显著差异,GHBP与身高生长速度(GV)、身高标准差分(HtsDs)、体重指数(BMI)呈正相关,GHBP与E_2、年龄(CA)、骨龄(BA)无相关关系。正常青春少女与ICPP女孩相应青春期血清GHBP无明显差异,ICPP女孩GHBP与HtsDs及BMI呈正相关关系,与E_2、CA及BA无相关关系。提示正常青春少女及ICPP女孩的生长,在GH轴调控途径上,至少在生长激素受体(GHR)/GHBP水平是相同的。GHBP受营养的正性影响,不受年龄、骨龄影响。     

西藏产茅膏菜化学成分的研究

目的:研究西藏产茅膏菜全草的化学成分。方法:采用pH梯度提取和柱层色谱法进行分离,根据化合物理化性质和光谱数据鉴定其结构。结果:得到5个结晶化合物,分别确定为矶松素(1),茅膏醌(2),羟基茅膏醌(3),β-谷甾醇(4),豆甾醇(5)。结论:化合物3和5为首次从该植物中分得。     

生姜解半夏毒的实验研究

小鼠腹腔刺激性实验结果表明,加用生姜可以降低半夏对小鼠腹腔的刺激性,减少扭体发生率;运用比色法和紫外分光光度法测定表明,生姜可以非常显著地抑制生半夏所致动物毛细血管通透性的增加及炎症足组织PGE_2的含量,降低炎症足的肿胀程度;增加动物胃液中PGE_2含量,拮抗生半夏所致动物胃液中PGE_2的含量降低,保护胃粘膜。提示生姜具有在机体内拮抗半夏的毒性作用。