三七总皂苷对大鼠脑缺血再灌注损伤血清IL-8的影响

目的 :研究三七总皂苷对脑缺血再灌注损伤血清IL - 8的影响。方法 :线栓法制备大鼠大脑中动脉阻断 (middlecerebralarteryocclusion ,MCAO)局灶性脑缺血模型 ,缺血 2h ,再灌注 3h ,放免法测定血清IL - 8的含量。结果 :5 0mg/kg-1ip ,qd× 7d能降低大鼠脑缺血再灌注后血清IL - 8的含量。结论 :三七总皂苷通过降低大脑缺血再灌注后血清IL - 8的产生和释放 ,对脑缺血再灌注损伤有保护作用。     

蝉蜕的甲壳质和壳聚糖的研究

蝉蜕的甲壳质和壳聚糖的研究杨其 ，黄金城，何蔚珩，梁诗 （广东医药学院广州５１０２２４）甲壳质和壳聚糖，国内外采用虾、蟹和昆虫的外壳等材料，进行工业化生产，供医药、化妆品和食品工业等广泛应用。作者从具有散风除热、解痉的中药蝉蜕ＰｅｒｉｏｓｔｒａｃｕｍＣ...     

斜外侧入路和经椎间孔入路椎间融合术在腰椎退变性侧凸的疗效比较

目的 比较斜外侧椎间融合术(oblique lumbar interbody fusion,OLIF)和经椎间孔椎间融合术(transforminal lumbar interbody fusion, TLIF)治疗腰椎退变性侧凸的临床疗效。方法 回顾性分析2015年1月至2018年5月治疗116例腰椎退变性侧凸合并椎管狭窄症患者资料。根据手术方式,将患者分为两组,其中56例患者采用OLIF入路[OLIF组,男21例,女35例;年龄(65.2±8.7)岁;Lenke-SilvaⅡ型41例,Ⅲ型15例]和60例采用TLIF入路[TLIF组,男19例,女41例;年龄(61.3±11.6)岁;Lenke-SilvaⅡ型43例,Ⅲ型17例]。比较两组手术前后视觉模拟评分(visual analogue scale, VAS)、Oswestry功能障碍指数(Oswestry disability index, ODI)评分及并发症;分析两组的各自手术前后的腰椎矢状位、冠状位Cobb角和顶椎中心偏移距离。结果 OLIF组和TLIF组VAS评分分别由术前的(7.7±1.6)分和(8.1±1.2)分降...     

多节段腰椎融合内固定术后早期腰背肌锻炼时机的效果研究

目的探讨多节段腰椎融合内固定术后早期腰背肌锻炼时机的康复效果。方法采用方便抽样法,选取2020年4-9月入住我院脊柱外科行多节段腰椎融合内固定术的患者共75例作为研究对象,采取先后入院非平行对照的方法将75例患者按入院时间先后分为2组,观察组(2020年4-6月)40例,术后3周开始进行五点式腰背肌锻炼;对照组(2020年7-9月)35例,术后6周开始进行五点式腰背肌锻炼。(1)比较2组患者出院前和术后3个月复查时的表面肌电信号均方根值(RMS)值及视觉模拟评分法(VAS)评分。(2)比较2组患者术前1日和术后3个月复查时腰椎功能障碍(ODI)评分。(3)观察并记录2组患者不良事件发生情况。结果 (1)2组患者出院前表面肌电信号RMS值及VAS评分比较差异无统计学意义(P>0.05);术前1日ODI评分比较差异无统计学意义(P>0.05)。(2)术后3个月复查时,2组患者表面肌电信号RMS值、VAS及ODI评分比较,差异均有统计学意义(P<0.05)。(3)2组患者术后3个月复查时,未发生内固定松动、断裂、移位及椎体不稳等不良事件。结论多节段腰椎内固定术后3周开始腰背肌锻炼,可以明显改善患者的腰椎功能,且不会增加不良事件的发生,其安全有效,值得临床推广。     

三七总皂苷对大鼠心肌肥厚的保护作用

目的研究三七总皂苷(total sapon ins of Panax notoginseng,PNS)对异丙肾上腺素所致大鼠心肌肥厚的保护作用。方法用异丙肾上腺素(isoproterenol,ISO)5 mg.kg-1.d-1,sc,连续7 d,建立大鼠心肌肥厚模型。造模第2天开始给大鼠腹腔注射PNS25和50 mg.kg-1.d-1,连续14 d,测定全心重量指数(HW/BW),左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量和一氧化氮合酶(NDS)活力;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果ISO模型组大鼠的HW/BW,LVI明显升高;左心室Hyp、AngⅡ含量增高;NO水平下降,诱生型NOS(iNOS)活力显著升高,结构型NOS(cNOS)活力显著降低。PNS能明显提高心肌组织NO水平和cNOS活力,降低胶原的含量及iNOS活力,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚。结论PNS对ISO所致大鼠心肌肥厚具有保护作用。     

三七总皂苷对大鼠心肌肥厚的保护作用

目的:研究三七总皂苷(total saponins of panax notoginseng, PNS)对异丙肾上腺素所致大鼠心肌肥厚的保护作用.方法:背部皮下注射用异丙肾上腺素(isoproterenol, ISO)5 mg/(kg·d),连续7 d,建立大鼠心肌肥厚模型.造模第二天开始给大鼠腹腔注射PNS 25和50 mg/(kg·d),连续14 d,测定全心重量指数(HW/BW),左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量.结果:ISO模型组大鼠的HW/BW、LVI明显升高;左心室HyP、AngⅡ含量增高,NO水平下降.PNS能明显提高心肌组织NO水平,降低胶原的含量,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚.结论:PNS对ISO所致大鼠心肌肥厚具有保护作用.     

腰椎融合术后腰背肌锻炼效果评价的研究进展

腰椎融合术后腰背肌锻炼可改善腰椎功能,减少残余症状,其临床应用近年来越来越受到重视.目前腰背肌锻炼康复效果的评价指标较多,常用的指标包括Oswestry功能障碍指数(Oswestry disability index,ODI)、疼痛视觉模拟量表(visual analogue scale,VAS)评分、腰椎日本骨科学会...     

COA:结缔组织生长因子促进半月板无血管区损伤愈合

Background: Connective tissue growth factor (CTGF) is a secreted protein containing several domains that mediate interactions with growth factors, integrin and extracellular matrix components. It plays an important role in extracellular matrix production by its ability to mediate collagen deposition during wound healing. CTGF also induces neovascularization in vitro, suggesting a role in angiogenesis in vivo. We herein report for the first time to test whether CTGF is required for extracellular matrix synthesis of meniscal fibrochondrocytes and/or angiogenesis during the repair of meniscal tearing damage. Methods: Meniscal fibrochondrocytes were isolated from the inner-1/2 of rabbits’ meniscus by trypsine collagenase treatment, and treated with 100ng/ml CTGF in vitro. Characterization of fibrochondrocytes was identified by flow cytometry analysising CD31, CD44, CD45 and CD105, and was further tested by type II collagen Immunocytochemistry. Changes in gene expression of meniscal fibrochondrocytes were monitored by quantitative real-time polymerase chain reaction. In vivo, the sections of the 3mm of the longitudinal tearing defect in the middle of the rabbit’s meniscus after treatment of 1.5ug of CTGF, which were subjected to fluorescence-immunohistochemisty analysis at the 1st week, the 4th week and the 10th week. Results: Quantitative RT-PCR assays showed that type I collagen, type II collagen, and VEGF mRNA expression in the 100ng/ml of CTGF group have been remarkably enhanced than in the no-dose group at the fourteen day, 2.38±0.63fold, 2.96±0.87fold, 2.14±0.56fold respectively. Consistent with these effects in vitro, fluorescence -immunohistochemical analysis revealed that in the group implanted with CTGF-fibrin glue, type I collagen, type II collagen and capillaries completely filled the defect at the 10th week postoperatively. In contrast, only soft tissue repair occurred when the PBS- fibrin glue was implanted. Conclusions: These findings suggest that CTGF can significantly promote extracellular matrix (I collagen, II collagen) of the meniscal avascular zone synthesis, and CTGF can greatly heighten the expression of VEGF activity at the same time in vivo, so that it can further enhance the repair of meniscal tearing damage in the avascular zone.     

药理学教学及考核方式改革的几种方法

目的:探索药理学教学改革以及相适应的考核方式改革的方式,方法和可行性.方法:结合我院学生特点并依据教学大纲的要求,我们从课堂理论和实验的教学模式、知识更新、考试制度等方面提出了药理学教学及考核方式改革的必要性和意义,并结合学校的具体情况,提出了相应的改革办法.