基于空洞卷积神经网络的心律失常分类算法

本文提出了一种基于卷积网络的心电信号分类算法,设计了空洞卷积池化金字塔模块,通过不同尺寸的空洞卷积提取信息,再将各通道的信息聚合,在增强网络的特征提取能力的同时可以降低参数量。本文聚焦于窦性心律、房性早搏、心动过速以及心动过缓4种分类,使用的心电图数据集来自医院的实测数据,数据集包含75000名不同检测者的心电记录。经过测试,本文提出的模型在该数据集上取得了0.89的F1值,另外在CinC2017数据集上也达到了0.87的F1值。实验结果表明该分类算法具有优秀的特征提取和分类能力,在心电信号的实时分类中具备应用前景。     

双苓扶正抗癌胶囊对小鼠肝癌的影响

目的：研究双苓扶正抗癌胶囊对小鼠肝癌的抑制作用及作用机制。方法：建立小鼠肝癌（Heps）实体瘤及腹水癌模型，观察抑瘤率和生长延长率，并用流式细胞术（FCM）检测小鼠肝癌实体瘤的细胞周期及癌细胞凋亡率。结果：双苓扶正抗癌胶囊在15g和30g/kg剂量下，对小鼠肝癌实体瘤有显著抑制作用，也能延长荷腹水型肝癌小鼠的生存期；还可引起小鼠肝癌瘤细胞S期及G2-M期细胞减少，G0-G1期细胞及凋亡细胞增加。结论：双苓扶正抗癌胶囊对小鼠移植性肝癌具有抑制作用，其作用机制可能与抑制细胞周期中S期DNA合成、干扰细胞的分裂增殖速度及诱导癌细胞凋亡有关。     

薄层扫描法测定黄芪配方颗粒中黄芪甲苷的含量

目的：对黄芪配方颗粒进行含量测定。方法：采用薄层扫描法对黄芪配方颗粒中的黄芪甲苷进行含量测定。结果：该方法的线性范围为0．78μg～4．68μg，平均回收率为99．06％，RSD=1．17％，(n=5)。结论：方法可行、重复性好，样品处理简单，操作方便，便于快速、准确的检测。     

阳离子脂质体介导BFGF/GFP基因对药物性耳蜗损害的防治作用

目的 探讨阳离子脂质体(天然碱性脂SA)携带碱性成纤维细胞生长因子/绿色荧光蛋白(bFGF/GFP)基因在豚鼠耳蜗中的表达,以及对庆大霉素所致耳蜗损害的防治作用。方法 将36只豚鼠分为3组,预防组右耳园窗注入SA-bFGF/GFP复合物后次日肌肉注射庆大霉素150mg.Kg~(-1).d~(-1)8天,治疗组先用庆大霉素8d后次日右耳给药,对照组单用庆大霉素8d。分别于实验前后及处死前行听觉脑干诱发电位(ABR)测试。荧光显微镜下观察耳蜗GFP的表达;用耳蜗琥珀酸脱氢酶染色铺片,扫描电镜观察毛细胞的缺失情况。结果 荧光显微镜下见双侧耳蜗均有GFP表达。预防和治疗组处死前的双耳ABR阈值与对照组比较差异有显著意义(P<0.01,P<0.05),耳蜗内外毛细胞缺失数与对照组比较差异有显著意义(P<0.01,P<0.05)。结论 SA脂质体介导的bFGF/GFP基因单耳给药双侧耳蜗均有高效表达,并对庆大霉素所致的耳蜗损害有防治作用。     

耳鸣的心理学问题

目的：分析耳鸣的心理原因和结果，以期引起临床医生的重视和研究。方法：对225例以耳鸣为第一主诉的主观耳鸣患者，采用唔谈，耳鸣分类调查表，耳鸣问卷，Zuang抑郁量表等进行心理问题评定，结果：全部患者均存在明显的心理问题，心理因素引起的耳鸣占21.0%，耳鸣引起的心理反应占67.7%，分不清先后（混合性）占11.3%。结论：耳鸣与心理因素密切相关，耳鸣问卷和耳鸣习服疗法能较好地测量和治疗患者的心理问题。     

Basic fibroblast growth factor protects auditory neurons and hair cells from noise exposure and glutamate neurotoxicity

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柯萨奇病毒B_3与亚急型克山病的关系

目的　探讨柯萨奇病毒B3(CVB3)在亚急型克山病发生发展中的作用。方法　采用原位核酸杂交和反转录 聚合酶链反应 (RT PCR)技术对吉林省病区亚急型克山病尸检心肌组织石蜡包埋块进行检测 ,并对所得DNA片段进行核苷酸序列分析。结果　 9例吉林省病区亚急型克山病尸检病例中有 7例出现原位核酸杂交结果阳性 ,阳性率为 78% ,对RT PCR所得DNA片段进行核苷酸序列分析发现它与CVB3有心肌毒性毒株 (CVB30 /Se-)的相应片段序列相同。结论　亚急型克山病病人心肌组织中有柯萨奇病毒B3感染 ,柯萨奇病毒B3参与克山病的发病过程。     

氢化可的松-羟丙基-β-环糊精包合物制备工艺的研究

目的:制备氢化可的松-羟丙基-β-环糊精包合物(HC-HP-β-CD),并初步考察其溶解性。方法:采用超声法制备HC-HP-β-CD,并利用正交实验设计筛选出包合物制备的最佳工艺,测定了包合物及HC的溶解度,比较了二者的紫外吸收光谱变化、X-射线衍射图谱、差示扫描量热图谱以及其在水中的溶解性。结果:通过比较包合物中药物浓度大小,确定包合最佳条件为HP-β-CD与HC比率为2:1,包合时药物浓度为3mg·mL~(-1),包合时间为20min。包合后HC的晶体衍射峰消失,差示扫描量热法测定结果显示形成一种新物相,使氢化可的松的溶解度增大了267倍,且常温下稳定性好。结论:HC与HP-β-CD形成新物相,明显提高了HC的水溶性,制成注射剂可避免通常普通制剂中的溶媒乙醇对机体的不良刺激。     

超声治疗外阴白色病变30例临床分析

目的 :探讨超声治疗外阴白色病变的临床疗效。方法 :收治外阴白色病变 6 0例 ,随机分为 :超声治疗组 30例 ,微波治疗对照组 30例。观察其临床症状、体征和组织学变化。结果 :超声治疗组 30例患者的外阴瘙痒症状基本消失 ,患处上皮不同程度地恢复正常 ,显效率达 90 %。不同的病理类型间疗效的比较 ,差异无显著性 (P >0 .0 5 )。结论 :用超声治疗外阴白色病变效果明显     

固体分散体中葛根素与磷脂的相互作用

目的　研究葛根素与磷脂在固体分散体中的作用。方法　溶剂挥发法制备固体分散体 ,通过紫外、红外、核磁共振光谱和薄层层析研究了葛根素与磷脂在固体分散体中的作用。结果　在氯仿中葛根素及其物理混合物的最大紫外吸收波长为 2 4 3nm ,但固体分散体为 2 5 0nm ,而在甲醇中都为 2 5 0nm。与物理混合物相比 ,固体分散体中葛根素苯环骨架的特征吸收峰和磷脂P =O伸缩振动峰明显改变 ,磷脂的C =C伸缩振动峰消失 ;在核磁共振光谱中固体分散体中葛根素苯环骨架的信号明显减弱或消失 ,而磷脂极性端和不饱和部分的信号明显变化。在以氯仿甲醇水 ( 6 5∶2 5∶5 )为展开剂的硅胶薄层板上葛根素与磷脂可完全分开。结论　在固体分散体中葛根素的苯环骨架与磷脂极性端和不饱和部分可能以氢键、静电引力或电荷迁移力以及分子间作用力相作用而固定 ,并被磷脂的脂肪链所包围