癌胚抗原DNA疫苗对大肠癌特异性细胞免疫的激活效果

目的检测以质粒pIRES为载体构建的带有全序列癌胚抗原(CEA)基因的核苷酸疫苗对机体特异性抗肿瘤免疫反应的激活效果。方法将CEA基因片段连接于真核表达质粒pIRES中,用肌肉注射方法接种核酸疫苗;检测CEA在小鼠肌肉组织中的表达情况及其对小鼠脾细胞CEA特异性细胞免疫反应的激活效果。结果小鼠经肌肉注射质粒后,免疫组化证实该核酸疫苗可在体内有效表达CEA;分子免疫检测显示注射后小鼠特异性淋巴细胞增值反应明显并且伴有自然杀伤细胞NK活性显著增高。结论实验所构建的核酸疫苗pIRESCEA可在体外及小鼠体内高效表达并表现出良好的细胞免疫原性。     

110例胃癌外科治疗分析

目的　探讨胃癌外科治疗的现状。方法　对我院 2002 年 5 月~2004 年 7 月收治并手术治疗的胃癌 110 例的临床资料进行回顾性分析。结果　110 例胃癌中,手术切除 101 例(91 .82%),其中根治性切除91例,姑息性切除10 例;远侧胃切除 78 例(77 .23%),近侧胃切除 7 例(6 .93%),全胃切除16例(15 .84%);行消化道重建的 104 例中,手法吻合 13 例(12 5%),单吻合器67例(66. 42%),双吻合器 24 例(23. 08%)。术后病理早期癌 9 例(8. 18%),进展期胃癌共 101 例(91 .82%)。术前肠外营养支持5~7天14例(12. 73%),术后行肠道营养86 例(85 15%),肠外营养90例(89 .11%)。手术死亡1例(0 .9%),12例发生术后近期并发症(9. 09%)。结论　胃癌患者早期诊断仍困难,但手术切除率及根治性切除率较以前明显升高,吻合器使用明显增加,有助于降低近期并发症发生率并提高肿瘤治疗的效果。需长时间营养支持时肠内营养较肠外营养更容易维持内环境稳定。     

Narrowband UVB therapy for vitiligo: does the repigmentation last?

BACKGROUND: Since 1997, a number of trials have shown promising results in treating generalized vitiligo with narrowband ultraviolet B (UVB) both in adults and children. However, there is little knowledge concerning the duration and permanency of the treatment-induced repigmentation. OBJECTIVE: Our main objective was to perform a follow-up trial of successfully treated patients receiving narrowband UVB for generalized vitiligo. METHODS: We have investigated to what degree the treatment-induced repigmentation remains stable for up to 2 years post-treatment. We performed an initial open trial including 31 patients with generalized vitiligo. They received narrowband UVB thrice weekly for up to 12 months. Patients experiencing > 75% repigmentation were defined responders and were included in the follow-up trial. Responders were followed every 6 months for up to 2 years after cessation of treatment. We observed the pigmentation status and registered any changes indicating loss of pigmentation and relapse. RESULTS: Eleven of the 31 treated patients were included in the follow-up trial. Six patients had relapse and five patients had stable response 24 months after cessation of treatment. Four out of six relapses were within 6 months post-treatment. CONCLUSION: In our study population of 31 patients with generalized vitiligo, five patients (16%) experienced > 75% stable repigmentation 2 years after cessation of a treatment programme of up to 1 years narrowband UVB therapy.     

吗啡成瘾性大鼠脑内核团神经细胞[Ca 2+]i变化的研究

目的 研究吗啡成瘾对大鼠脑内与成瘾相关核团神经细胞[Ca 2 ]i的影响.方法 将50只SD大鼠随机分成吗啡成瘾组和对照组,观察成瘾后的戒断症状;应用对Ca2 敏感的探针Fluo-3与Ca2 络合后被激光激发发出荧光的特性,利用激光共聚焦显微镜观察伏核、海马、内侧额前皮质神经细胞[Ca 2 ]i变化,并用图像分析软件进行处理.结果 吗啡成瘾大鼠脑内伏核、海马及内侧额前皮质神经细胞[Ca 2 ]i和对照组比较明显增高(P<0.01).结论 长期使用吗啡可明显增高伏核、海马和内侧额前皮质神经细胞[Ca 2 ]i.     

Effects of hypoxic preconditioning on the changes of expression of neuroglobin mRNA and labeled positive cells following cerebral ischemia in gerbils

INTRODUCTION Neuroglobin (NGB) is a recently discovered member of the vertebrate globin family [1], predominantly expressed in various brain regions of human and vertebrate. The expression of NGB was increased in case of ischemia, which may play a role in…     

Neuroimaging in Pineal Tumors

BACKGROUND AND PURPOSE: The authors report radiological findings in 11 tumors in the pineal region, which were histologically diagnosed as germinomas, pineocytomas pineoblastomas, ependymomas, teratomas, and astrocytomas. METHODS: Computed tomography (CT) was performed in seven patients and magnetic resonance imaging (MRI) was performed in all patients. RESULTS: CT showed a solid or solid/cystic mass with variable contrast enhancement. MRI showed a heterogeneous mass, with hypointense signal on T1 and iso/hyperintense signal on T2-weighted images (WI) and gadolinium enhancement. Extension to adjacent structures occurred in five patients and spread through the cerebral spinal fluid (CSF) in two. CONCLUSIONS: Pineal region tumors have no pathognomonic imaging pattern. MRI and CT are complementary in diagnosis and are important to determine localization, extension, and meningeal spread.     

输尿管镜下气压弹道碎石治疗输尿管结石失败原因探讨(附36例报告)

目的:探讨输尿管镜下气压弹道碎石(URSL)治疗输尿管结石失败原因及防治措施.方法:回顾分析36例气压弹道碎石治疗输尿管结石手术失败的临床资料.结果:17例手术中结石移位进入肾盂,无法碎石;8例因输尿管走行迂曲或输尿管狭窄置镜未成功;5例因局部出血或引流不畅导致视野不清终止手术;6例因输尿管穿孔而改开放手术.结论:结石移位、输尿管走行迂曲或狭窄、术中视野不清、输尿管穿孔是手术失败的主要原因.合理选择病例,保持液适当的灌注速度和压力,保持视野的清晰和在直视下推进输尿管镜是手术成功的关键.     

P17 Pharmacology in the integrated course ＂Basic Medical Sciences＂ in Zhejiang University School of Medicine

According to traditional teaching mode, the courses in preclinical medicine including pharmacology are separately run. This mode causes a series of disadvantages including loose connection between knowledge in different disciplines and weak ability to bridge basic preclinical knowledge and clinical practice. In order to overcome the disadvantages and promote the teaching efficiency, we constructed a new integrated course-Course of Basic Medical Sciences, which includes 6 traditional courses, anatomy, histology and embryology, physiology, pathology, pathophysiology and pharmacology. We integrated these courses based on the human organ systems and according to the principle-＂ From macro to micro, From morphological to functional, From normal to abnormal and From disease to drug therapy＂ and published the series of textbook in 2004. The contents of pharmacology are taught just after pathology and pathophysiology in every organ system. In comparison with the traditional teaching mode, teachers of pharmacology need not spend a lot of time to review preceding knowledge of anatomy and histology, physiology, pathophysiology and pathology. This is helpful in saving time and improving the teaching efficiency.