利用逆转录聚合酶链反应及DNA印迹杂交技术检测乳腺癌腋窝淋巴结微转移

目的　评价检测原发性乳腺癌腋窝淋巴结微转移的方法。方法　用逆转录聚合酶链反应(RT-PCR)和Southern杂交方法，检测腋窝淋巴结中CK-19基因表达。同时与免疫组织化学(组化)方法比较其检测敏感性。结果　RT-PCR、Southern杂交及免疫组化方法的检测敏感性分别为1∶5×10     

小鼠抗人雌激素受体抗血清的制备及应用

作者以人雌激素受体Ｄ段（ＥＲ－Ｄ）含１５个氨基酸的合成肽与匙孔血蓝蛋白（ＫＬＨ）的交联物为抗原免疫雄性小鼠，制备抗ＥＲ－Ｄ抗血清，并以此用免疫组织化学（ＩＨＣ）技术对９５例经葡聚糖包裹活性碳（ＤＣＣ）法分析的乳腺癌组织切片进行ＥＲ检测。同时，首次采用地高辛配基标记探针的非同位素原位杂交（ＩＳＨ）技术，检测了１５例同一批标本的ＥＲｍＲＮＡ表达水平。实验结果显示：ＩＨＣ和ＤＣＣ之间，其阳、阴性符合率为９０％，相对于ＩＨＣ的半定量分级而言，其程度符合率为６９％，ＩＨＣ与ＩＳＨ的阳、阴性符合率达１００％。因此，作者认为，抗ＥＲ－Ｄ抗血清的ＩＨＣ法可以代替或辅助ＤＣＣ法用于临床乳腺癌ＥＲ的检测。     

开原市农村碘缺乏病区地方性亚临床克汀病流行程度调查

目的 了解开原市农村供应同浓度(1：5万)碘盐后重、中度碘缺乏病区出生儿童的亚克病流行程度。方法用中国联合型瑞文测验(CRT-C2)测定7～14岁儿童智商(IQ)，用津医精神运动成套测验(TPB)、测听仪、拍X线片，标准度衡器具检出IQ50～69儿童的精神运动、听力障碍和骨龄、体格发育落后者。结果 重、中度病区出生儿童的人群轻度智力落后率分别为7．6％、5．9％，轻度智力落后儿童的亚临床损伤率分别为78．3％、77.4％，重、中度病区儿童亚克汀病患病率分别为6％、4．6％。结论 同浓度碘盐预防后重度病区儿童亚克病流行程度较中度病区为重；供应碘盐合格率低，孕妇摄碘不足，致使病区仍有亚克汀病儿出生。     

全身照射后行自家骨髓移植的恶性淋巴瘤2例报告

本文报告2例骨髓未受浸犯的恶性淋巴瘤病例,为达到根治目的,将其自身骨髓细胞冷冻保存,于全身照射治疗后再行骨髓移植。例1,27岁男性,1979年6月右耳下淋巴结肿大,渐而扩散至全身,并有类白血病性反应。7月16日入院,经淋巴结活检诊断为淋巴瘤((?)期)。骨髓活检两处确认骨髓未受累。8月9日在全身麻醉下,采取5.7     

应用逆转录-聚合酶链反应技术检测乳腺癌骨髓微转移

我们利用逆转录 聚合酶链反应(ＲＴ ＰＣＲ)技术 ,以上皮源性细胞特异性标志基因—细胞角蛋白 19(ＣＫ 19)的表达为标志物 ,对我院外科收治的 5 4例可手术的原发性乳腺癌患者的骨髓 ,进行了微转移的检测 ,并结合其它参考指标探讨其临床意义。1.资料与方法 :(1)临床资料 :本组患者 5 4例 ,均为女性 ,年龄 2 6 0～ 75 0岁 ,平均 5 2 6岁。绝经前 18例 ,绝经后36例。手术方式 :根治术 2例 ,改良根治术 5 1例 ,1/ 4乳腺切除加腋清扫 1例。肿瘤大小 :直径≤ 2 0ｃｍ 2 3例 ,2 1～ 5 0ｃｍ 2 0例 ,>5 0ｃｍ 11例。激素受体检查 :检查 48例 …     

抗p53融合蛋白单克隆抗体的性质鉴定

目的 :研制出能特异与p5 3结合的单克隆抗体 ,使对 p5 3的检测得到更为广泛的应用。 方法 :以原核表达的 p5 3-GST融合蛋白为免疫原 ,常规方法作细胞融合 ,间接酶联免疫吸附试验 (ELISA)双筛和免疫组织化学(IHC)筛选 ,将能稳定分泌抗人p5 3单抗的杂交瘤细胞株的细胞上清 (命名为M12 6 )与常用p5 3进口单抗PAB180 1(ZYMED公司 )作对比实验。结果 :此单抗适用于ELISA、IHC、免疫印迹及免疫沉淀等方面的研究 ,并且在一定程度上优于PAB180 1。结论 :新制备的单抗M12 6可考虑取代进口单抗PAB180 1而用于p5 3的表达及突变研究。     

辽宁省碘缺乏病病区不同补碘时期出生儿童智力水平调查

objecfive To know and compare the intelligence level of children born in different time periods in regions with iodine deficiency disorders(IDD)in Liaoning province.Methods All 7-14 year-old children from ten schools were chosen as the subjects respectively from six villages in each of the six counties and in regions with iodine deficiency,who were respectively born at the initialization of iodinated salt supplying period(1978-1980);non-iodinated salt supplying period(1981-1990);recovery of supplied iodized salt period(1991-1995);universal iodized salt period(1996-2000),respectively.Intelligence quotient(IQ)was measured by Combined Ravens Test in China(CRT-C)and Combined Ravens Test-the Rural,in China,2nd edition(CRT-RC2).Results IQ of children during the non-iodized salt period(91.9±14.3)was significantly lower than the initial supply of iodized salt period(95.8±14.6,q=8.60,P<0.01),recovery of supplied iodized salt period(99.7±14.7)was significantly higher than the initial supply of iodized salt period, non-iodized salt sales period(q = 9.53, 18.13, all P < 0.01 ),universal salt iodization( 104.3 ± 14.9) was significantly higher than the initial supply of iodized salt period, non-iodized salt sales period, recovery of supplied salt iodization(q = 20.00,28.00,10.46, all P < 0.01). Children's rate of mental retardation (IQ≤69) was higher in non-iodinated salt supplying period (6.7%, 88/1314 ) than the initial supply of iodized salt (4.4%, 21/471, χ2 = 3.85, P < 0.05), recovery of supplied iodized salt period(3.3%,48/1470) was significantly lower than non-iodinzed salt supplying period (χ2 = 15.37, P < 0.01), universal salt iodization period(2.7%, 36/1344) was lower than the initial supply of iodized salt period(χ2 = 4.41, P < 0.05) and non-iodinzed salt supplying period(χ2 = 26.34, P < 0.01 ). The IQ and intelligent retarded rates in children born during the initial years of iodinated salt supplying period were not different. The IQ of the children during ten years of non-iodized salt supplying period fluctuated in a "∪" curve, while the intelligent retardation rates in a "∩" curve.The children born during the period of recovery supplied iodized salt increased their IQ and lowered the retardation rates year after year. The IQ of the children in universal iodized salt period kept on increasing while intelligent retarded rates reduced to the lowest level. Conclusions The intelligence level of children born in regions with IDD during non-iodized salt supplying period is remarkably lower than that of the beginning years of iodinated salt supplying period. The intelligence level of children born after universal iodized salt period is remarkably higher than that of the initial iodinated salt supplying period and recovery of supplied iodized salt period, respectively.     

逆转录-聚合酶链反应联合检测乳腺癌腋窝淋巴结和骨髓的微转移

目的：检测原发性乳腺癌常规病理检查一淋巴结和骨髓中微转移琢与其它临床参考指标的关系。方法：利用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）联合检测腋窝淋巴结和骨髓中细胞是白１９（ＣＫ－１９）基因表达。将乳腺癌细胞株（Ｔ４７Ｄ）与骨髓细胞按不同比例混合，比较ＲＴ－ＰＣＲ与免疫组化（ＩＨＣ）方法的检测敏感性。结果：３５例乳腺癌共１３７枚淋巴结有８例１１枚ＣＫ－１９表达阳性；３５份髓标本，其中８份ＣＫ－１９表达     

补碘后出生儿童骨龄和体格发育与智商水平的关系

目的：了解重,中度碘缺乏病区供应碘盐后出生儿童的骨龄和体格发育状况与智商水平的关系。方法：CRT－C2测7－14岁儿童智商,拍手腕骨X线片判定骨龄,标准量具测身高,体重,结果：碘盐预防后出生儿童的骨龄,身高,体重发育落后率均随人群智商（IQ）水平提高而下降,IQ50－69组儿童骨龄,身高,体重落后率分别为18．3％,17．6％,10．2％,均显著高于IQ70－79,IQ80－119,IQ大于等于120三组儿童（P＜0．01）,轻度智商落后组儿童的骨龄发育落后以尺骨未按时出现为常见,体格发育障碍为身高落后者明显多于体重落后者,骨龄与身高发育落后程度一致。结论：补碘后碘缺乏病区儿童骨龄和体格发育与智商水平密切相关,轻度智商落后组儿童的骨龄,身高,。体重 状况显著落后于同期出生智商水平较高组儿童。     

抗HRP单克隆抗体及PAP复合物活性影响因素的研究

作者制备了大鼠抗辣根过氧化物酶单克隆抗体及其与辣根过氧化物酶的复合物,观察了温度(室温、4℃、-20℃、-70℃)、pH值(pH=3,pH=11),蛋白稳定剂(1％BSA,50％中性甘油)及保存时间对抗体活性的影响。以ELISA法及微孔免疫细胞化学法检测抗体活性。结果表明;含此抗体的腹水及培养上清在室温中放置2周,4℃、-20℃、-70℃中放置10个月,抗体活性未见明显改变,加与不加蛋白稳定剂无明显差异。此培养上清在酸性环境(pH=3)中24h,活性严重受损,而其中含1％BSA及腹水者可耐受24h,活性无明显变化。此抗体与酶的复合物在4℃、-20℃、-70℃保存1年,仍保持良好的染色效果,与开始相比无显著性差异。