Toll样受体9基因启动子单核苷酸多态性与儿童变应性哮喘的相关性

Objective To investigate the distribution characteristics of the single nucleotide polymorphisms (SNPs) in the promoter region of the toll-like receptor 9 gene (TLR9)in Chinese Han children from Zhejiang province, and their associations with asthma susceptibility and phenotypes. Methods A case-control study was conducted. A total of 312 asthmatic children aged between 1.9 and 11.6 and 339 age matched healthy controls were enrolled in this study from April 2007 to November 2008. The -1486 C/T in rs187084 and -1237 C/T in rs5743836 loci of the TLR9 gene were genotyped by direct DNA sequencing of the PCR products. Serum levels of IFN-γ, IL-12 and IL-4 were detected by enzyme linked immunosorbent assay. Serum levels of total IgE were detected by chemiluminescence, and serum levels of ildren (P＜0.01). The CC genotype had the lowest levels of serum IFN-γand the highest levels of serum IL-4 among the three genotypes. There were no significant differences in these cytokines among the healthy controls (P＞0.05). No statistical differences of serum IL-12 were found among the three genotypes in the two groups (P＞0.05). (4) There were no significant differences of total IgE (log-transformed) among the three genotypes in the asthmatic children (P＞0. 05). Conclusion The -1237 C/T polymorphism of TLR9 gene was not detected in Chinese Han children in this study. The - 1486 C/T polymorphism was associated with the levels of serum IFN-γ and IL-4 in children with asthma.However, there were no correlations between the -1486C/T polymorphism and serum IL-12 levels, total IgE levels or asthmatic susceptibility.     

失眠患儿认知功能 睡眠参数及皮质醇水平变化50例临床分析

目的 通过分析失眠患儿睡眠结构和唾液皮质醇水平的变化及对认知功能的影响，探讨导致失眠患儿认知功能受损的影响因素。方法 2019年10月至2020年11月，在温州医科大学附属第二医院育英儿童医院选择符合《睡眠障碍国际分类第3版》诊断标准的50例失眠患儿为观察组；以20例同期体检健康儿童为对照组。两组均采用儿童睡眠紊乱量表评估主观睡眠质量，进行汉化版心理健康诊断测验认知（DN:CAS）评估系统评估认知功能，测定晨醒后30 min唾液的皮质醇水平；观察组患儿进行整夜多导睡眠监测仪（PSG）监测，记录睡眠参数。结果 观察组患儿在DN:CAS中数字匹配分测验得分显著低于对照组[（10.56±3.03）vs.(12.80±3.11),t=-2.78,P<0.01]；观察组在表达性注意分测验得分显著低于对照组[（10.00±2.41）vs.(12.45±3.20),t=-3.09,P<0.01]，数字检测分测验显著降低[（9.66±1.83）vs.(11.45±3.19),t=-2.10,P<0.05]。观察组唾液皮质醇水平显著高于对照组[（104.66±34.30）μg/L vs...