口服番泻叶致变态反应1例

番泻叶是一种常见的导泻药,在医院中常把它作为肠道检查前的清肠剂,或用来为部分急性便秘病人解除便秘之苦,具有用法简单、效果可靠、病人依从性好等优点.2008年9月我科1例病人口服番泻叶后出现变态反应.现报告如下.     

p21^WAF1和p53在宫颈鳞癌中的表达及意义

目的探讨p21WAF1和p53蛋白在宫颈鳞癌发生发展中的表达以及相互关系。方法采用免疫组化方法研究50例宫颈鳞癌和30例正常子宫颈组织p21WAF1和p53蛋白的表达。结果宫颈鳞癌组织中p21WAF1蛋白阳性表达率42.0%,明显低于正常对照组73.33%（P〈0.05）;p53蛋白的阳性表达率为54%,明显高于正常对照组6.67%（P〈0.05）。p21WAF1与p53表达存在明显负相关。结论 p21WAF1蛋白的低表达和p53蛋白的高表达与宫颈鳞癌的发生发展相关。     

Knowledge of emergency management of avulsed teeth: a survey of dentists in Beijing,China

Abstract – Objectives: There is a high frequency of dental trauma cases in China, where tooth avulsion is one of the most serious scenarios. The knowledge of how to take care of an avulsion is of great importance to the outcome of such an injury. This knowledge among dentists in Beijing, China has never been tested before. The purpose of this study was to evaluate professional experience and knowledge of emergency management of dentists working in urban and suburban areas of Beijing, China. Material and Methods: A two‐part questionnaire was distributed to 175 urban and 99 suburban dentists in Beijing. The first part contained personal and professional information of the participating dentists. The second part evaluated dentists’ knowledge about the emergency management of avulsed teeth. Data were entered into a SPSS database and analyzed using the chi‐square test and Fisher’s exact test for each question. Results: All dentists had a college or above degree. Almost five times as many urban dentists compared to suburban dentists presented with a Master or PhD degree. The knowledge levels of the participants presenting with correct answer in the respective knowledge areas were; storage medium (15.8%) intra‐canal medication (45.0%), type of splint (45.1%) and splinting period (10.2%). Significantly more urban dentists (50.6%) compared to suburban dentists (34.8%) (P = 0.016), used calcium hydroxide paste as an intracanal medicament, and significantly more urban dentists (51.3%) than suburban dentists (34.1%) chosed a flexible splint (P = 0.008). Conclusion: The results revealed an uneven pattern of knowledge between urban and suburban dentists regarding the emergency management of avulsed teeth. The study highlighted Chinese dentists’ need for continuing education in order to improve current knowledge in emergency management of avulsed teeth.     

Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein

An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) method was developed for the efficient detection of the UL24 antigen of the duck enteritis virus (DEV) using polyclonal antibodies. Ducks and rabbits were immunized, respectively, with expressed UL24 recombinant protein. The IgG antibodies against UL24 from ducks and rabbits were purified and used as the capture antibodies. The specificity of the optimized AC-ELISA was evaluated by use of DEV, duck hepatitis virus (DHV), duck hepatitis B virus (DHBV), gosling plague virus (GPV), Riemerella anatipestifer (R.A.), Escherichia coli (E. coli), Pasteurella multocida (P.M.) and Salmonella Enteritidis (S.E.). Only DEV specimens yielded a specific and strong signal. The limit of the sensitivity of this method for the detection of DEV was 46 ng/100 μl. Compared with PCR and virus isolation, the rate of agreement for the detection of experimentally infected sera was 100%. A comparative test used on clinical specimens between the neutralization test and the AC-ELISA showed that the proportions of true positives and true negatives by the AC-ELISA were 0.90 and 0.67 respectively. These results indicated that the AC-ELISA approach is rapid, sensitive, and reliable for specific detection of DEV antigen.