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101.
Zbyšek Pavelek Oldřich Vyšata Lukáš Sobíšek Blanka Klímová Ctirad Andrýs Doris Vokurková Radka Mazurová Pavel Štourač Martin Vališ 《Neurologia i neurochirurgia polska》2018,52(5):587-592
Background
The goal of this study was to determine the characteristics that are affected in patients treated with glatiramer acetate (GA).Methods
A total of 113 patients were included in this study. Patients were treated with glatiramer acetate (subcutaneous injection, 20 mg, each day). Peripheral blood samples were obtained just prior to treatment as well as 5 years after GA treatment. All the calculations were performed with the statistical system R (r-project.org).Results
After 5 years of treatment, a significant decrease was found in the absolute and relative CD3+/CD69+ counts, the absolute and relative CD69 counts, the relative CD8+/CD38+ count and the relative CD38 count. A significant increase was found in the absolute and relative CD5+/CD45RA+ counts and the absolute CD5+/CD45RO+ count after 5 years of treatment.Conclusion
This study presents some parameters that were affected by long-term GA treatment. 相似文献102.
Jong Woo Lee Yu Zhang Kyung Jin Eoh Roshan Sharma Miguel F. Sanmamed Jenny Wu Justin Choi Hee Sun Park Akiko Iwasaki Edward Kaftan Lieping Chen Vali Papadimitrakopoulou Roy S. Herbst Ja Seok Koo 《Journal of thoracic oncology》2019,14(6):1046-1060
IntroductionThis study aimed to characterize the tumor-infiltrating immune cells population in Kras/tumor protein 53 (Trp53)-driven lung tumors and to evaluate the combinatorial antitumor effect with MEK inhibitor (MEKi), trametinib, and immunomodulatory monoclonal antibodies (mAbs) targeting either programmed death -1 (PD-1) or programmed cell death ligand 1 (PD-L1) in vivo.MethodsTrp53FloxFlox;KrasG12D/+;Rosa26LSL-Luciferase/LSL-Luciferase (PKL) genetically engineered mice were used to develop autochthonous lung tumors with intratracheal delivery of adenoviral Cre recombinase. Using these tumor-bearing lungs, tumor-infiltrating immune cells were characterized by both mass cytometry and flow cytometry. PKL-mediated immunocompetent syngeneic and transgenic lung cancer mouse models were treated with MEKi alone as well as in combination with either anti–PD-1 or anti–PD-L1 mAbs. Tumor growth and survival outcome were assessed. Finally, immune cell populations within spleens and tumors were evaluated by flow cytometry and immunohistochemistry.ResultsMyeloid-derived suppressor cells (MDSCs) were significantly augmented in PKL-driven lung tumors compared to normal lungs of tumor-free mice. PD-L1 expression appeared to be highly positive in both lung tumor cells and, particularly MDSCs. The combinatory administration of MEKi with either anti–PD-1 or anti–PD-L1 mAbs synergistically increased antitumor response and survival outcome compared with single-agent therapy in both the PKL-mediated syngeneic and transgenic lung cancer models. Theses combinational treatments resulted in significant increases of tumor-infiltrating CD8+ and CD4+ T cells, whereas attenuation of CD11b+/Gr-1high MDSCs, in particular, Ly6Ghigh polymorphonuclear-MDSCs in the syngeneic model.ConclusionsThese findings suggest a potential therapeutic approach for untargetable Kras/p53-driven lung cancers with synergy between targeted therapy using MEKi and immunotherapies. 相似文献
103.
Mohsen Beheshti Sigrid Pöcher Reza Vali Peter Waldenberger Gabriele Broinger Michael Nader Susanne Kohlfürst Christian Pirich Henning Dralle Werner Langsteger 《European radiology》2009,19(6):1425-1434
The purpose of this prospective study was to compare the value of DOPA PET-CT with FDG PET-CT in the detection of malignant
lesions in patients with medullary thyroid carcinoma (MTC). Twenty-six consecutive patients (10 men, 16 women, mean age 59 ± 14 years)
with elevated calcitonin levels were evaluated in this prospective study. DOPA and FDG PET-CT modalities were performed within
a maximum of 4 weeks (median 7 days) in all patients. The data were evaluated on a patient- and lesion-based analysis. The
final diagnosis of positive PET lesions was based on histopathological findings and/or imaging follow-up studies (i.e., DOPA
and/or FDG PET-CT) for at least 6 months (range 6–24 months). In 21 (21/26) patients at least one malignant lesion was detected
by DOPA PET, while only 15 (15/26) patients showed abnormal FDG uptake. DOPA PET provided important additional information
in the follow-up assessment in seven (27%) patients which changed the therapeutic management. The patient-based analysis of
our data demonstrated a sensitivity of 81% for DOPA PET versus 58% for FDG PET, respectively. In four (4/26) postoperative
patients DOPA and FDG PET-CT studies were negative in spite of elevated serum calcitonin and CEA levels as well as abnormal
pentagastrin tests. Overall 59 pathological lesions with abnormal tracer uptake were seen on DOPA and/or FDG PET studies.
In the final diagnosis 53 lesions proved to be malignant. DOPA PET correctly detected 94% (50/53) of malignant lesions, whereas
only 62% (33/53) of malignant lesions were detected with FDG PET. DOPA PET-CT showed superior results to FDG PET-CT in the
preoperative and follow-up assessment of MTC patients. Therefore, we recommend DOPA PET-CT as a one-stop diagnostic procedure
to provide both functional and morphological data in order to select those patients who may benefit from (re-)operation with
curative intent as well as guiding further surgical procedures. 相似文献
104.
Robert M. Dorman Kaveh Vali Carroll M. Harmon Mario Zaritzky Kathryn D. Bass 《Pediatric surgery international》2016,32(5):525-528
We describe the treatment of a patient with long-gap esophageal atresia with an upper pouch fistula, mircogastria and minimal distal esophageal remnant. After 4.5 months of feeding via gastrostomy, a proximal fistula was identified by bronchoscopy and a thoracoscopic modified Foker procedure was performed reducing the gap from approximately 7–5 cm over 2 weeks of traction. A second stage to ligate the fistula and suture approximate the proximal and distal esophagus resulted in a gap of 1.5 cm. IRB and FDA approval was then obtained for endoscopic placement of 10-French catheter mounted magnets in the proximal and distal pouches promoting a magnetic compression anastomosis (magnamosis). Magnetic coupling occurred at 4 days and after magnet removal at 13 days an esophagram demonstrated a 10 French channel without leak. Serial endoscopic balloon dilation has allowed drainage of swallowed secretions as the baby learns bottling behavior at home. 相似文献
105.
Valić, F., and Žuškin, E. (1971).Brit. J. industr. Med.,28, 364-368. A comparative study of respiratory function in female non-smoking cotton and jute workers. To compare the effect of cotton and jute dust, respiratory symptoms were studied and respiratory function measured in 60 cotton and 91 jute non-smoking female workers of similar age distribution, similar length of exposure to dust, and exposed to similar respirable airborne dust concentrations. Cotton workers had a significantly higher prevalence of byssinosis, of persistent cough, and of dyspnoea (P < 0·01) than jute workers. Among cotton workers 28·3% were found to have characteristic symptoms of byssinosis, whereas none was found among jute workers.
Exposure to cotton but also to jute dust caused significant reductions of FEV1·0, FVC, and PEF (P < 0·01) over the first working shift in the week. Functional grading of jute and cotton dust effects has shown that about 30% of cotton workers had functional grades F1 and F2, while only 13% of jute workers were found in the same grades (F1). It is concluded that cotton dust may be considered more active than jute though the latter cannot be considered inactive.
相似文献106.
Ali R. Rezai Jesus F. Salazar-Gonzalez Otoniel Martí nez-Maza John Bramhall Rahmatollah Afrasiabi Vali Kermani-Arab 《Immunopharmacology and immunotoxicology》1990,12(3):345-362
Histamine inhibited the roliferative response of human peripheral blood mononuclear cells (PBMCT to the T cell mitoFen Phytohema P (PHA-P) in a dose-dependent fashion. This inhibition was mePutiated via the H2 receptor since cimetidine, a known H2 antagonist, removed the inhibition, whereas the addition of the H1 antagonist Diphenhdramine did not. Inhibition occurred durin the inductive phase of the cell cycle, since histamine added 24 hours aBter PHA-P stimulation had no effect on subsequent T cell proliferation, and was attributable to inhibition of interleukin-2 (IL-2) gene expression. Both secreted IL-2 and messenger RNA coding for IL-2 were inhibited by histamine. In contrast, histamne exerted no inhibitory effect on the expression of cell surface receptors for IL-2 as determined by flow cytometry. Furthermore, histamine-treated cells retained full responsiveness to exogenously administered IL-2, which completely reversed the anti-proliferative effect of histamine. In some donors, histamine enhanced the ercentage of IL-2 receptor positive cells. Stimulated PBMC from AIDS KS patients as a group, displayed a lower ercentage of IL-2 receptor bearing cells, which was significantly increased gy the addition of histamine even at concentrations as low as 10--6 M and peaking at 10--3 M. These findings indicate that histamine exerts its anti-proliferative effects on T cells by inhibiting IL-2 production, via blockade of IL-2 gene expression. In addition, histamine seems to exert immunomodulating effects on IL-2 receptor expression, particularly in those individuals with AIDS-KS. 相似文献
107.
Treatment and prevention of intraepithelial neoplasia: an important target for accelerated new agent development. 总被引:12,自引:0,他引:12
Joyce A O'Shaughnessy Gary J Kelloff Gary B Gordon Andrew J Dannenberg Waun Ki Hong Carol J Fabian Caroline C Sigman Monica M Bertagnolli Steven P Stratton Stephen Lam William G Nelson Frank L Meyskens David S Alberts Michele Follen Anil K Rustgi Vali Papadimitrakopoulou Peter T Scardino Adi F Gazdar Lee W Wattenberg Michael B Sporn Wael A Sakr Scott M Lippman Daniel D Von Hoff 《Clinical cancer research》2002,8(2):314-346
Precancer or intraepithelial neoplasia (IEN) is a noninvasive lesion that has genetic abnormalities, loss of cellular control functions, and some phenotypic characteristics of invasive cancer and that predicts for a substantial likelihood of developing invasive cancer. The AACR Task Force on the Treatment and Prevention of IEN has delineated the relationship between IEN and cancer risk as well as the clinical benefit that can be derived from reducing IEN burden. Although several effective endoscopic and surgical treatments for IEN have become standard medical practice, these interventions can confer morbidity and do not treat the entire epithelial field at risk. The incidence of many epithelial cancers is continuing to rise, the number of individuals at risk is increasing with the aging population, and the rapid advancement of imaging and molecular diagnostics is bringing to light precancers that were heretofore clinically silent. There is therefore an urgent need to rapidly develop new treatment and prevention agents for IEN. The AACR IEN Task Force recommends focusing on established precancers as the target for new agent development because of the close association between dysplasia and invasive cancer and because a convincing reduction in IEN burden provides patient benefit by reducing cancer risk and/or by decreasing the need for invasive interventions. The IEN Task Force proposes several clinical trial designs that provide practical and feasible approaches to the rapid development of new agents to treat and prevent precancer. 相似文献
108.
OBJECTIVE: This study investigated the longitudinal inter-relationships among the consumption of specific alcoholic beverages (beer, wine and distilled spirits), physical symptoms and psychological distress. Two causal processes were examined for each type of beverage: (1) the use of alcohol to cope with pre-existing psychological distress and physical symptoms; (2) the impairment effects of alcohol on subsequent physical and mental health. METHOD: Data were collected at three points in time (baseline, 3-year follow-up, 6-year follow-up) from a household sample of 1,270 youths who were ages 12, 15 and 18 at the baseline interview. RESULTS: Consumption of all three beverages increased through early adolescence and leveled off at ages 18 and 21, and males reported more beer use than females. Structural equation models showed that the use of all three alcoholic beverages contributed significantly to longitudinal increases in physical symptoms, but not to changes in psychological distress. In contrast, no evidence supported the coping hypothesis. Finally, there were no significant interaction effects involving beverage type, gender or age on physical symptoms. CONCLUSIONS: The results suggested that the physical impairment process operated similarly for beer, wine and distilled spirits, for males and females, and for adolescents of different ages. 相似文献
109.
Dunja Beritić-Stahuuak F. Valić Mira Cigula D. Butkovic 《International archives of occupational and environmental health》1976,37(3):193-203
Summary In order to estimate whether only simultaneous exposure to both airborne flour particles and unfavorable thermal environment alone cause the development of nonspecific respiratory disease or whether sole exposure to flour aerosols is likely to cause impairment of the respiratory system, a group of 80 millers and 130 bakers, all nonsmokers, were examined. Both millers and bakers were exposed to approximately the same concentration of airborne flour particles but the latter worked under considerable thermal load. It was found that between millers and bakers the difference in the prevalence of respiratory symptoms was not statistically significant. Although the differences between the mean measured and the mean predicted ventilatory function parameters (FVC, FEV1, PEF, MEF 50%, MEF 75%) were significant both in millers and bakers, they did not differ significantly between these two groups. This finding suggests that exposure to flour dust may cause the development of chronic nonspecific respiratory disease and a reduction of ventilatory lung capacity irrespective of simultaneous heat load. 相似文献
110.
Meghan E. Byrne David A. Ball Jean-Luc Guerquin-Kern Isabelle Rouiller Ting-Di Wu Kenneth H. Downing Hojatollah Vali Arash Komeili 《Proceedings of the National Academy of Sciences of the United States of America》2010,107(27):12263-12268
Intracellular magnetite crystal formation by magnetotactic bacteria has emerged as a powerful model for investigating the cellular and molecular mechanisms of biomineralization, a process common to all branches of life. Although magnetotactic bacteria are phylogenetically diverse and their crystals morphologically diverse, studies to date have focused on a few, closely related species with similar crystal habits. Here, we investigate the process of magnetite biomineralization in Desulfovibrio magneticus sp. RS-1, the only reported species of cultured magnetotactic bacteria that is outside of the α-Proteobacteria and that forms bullet-shaped crystals. Using a variety of high-resolution imaging and analytical tools, we show that RS-1 cells form amorphous, noncrystalline granules containing iron and phosphorus before forming magnetite crystals. Using NanoSIMS (dynamic secondary ion mass spectroscopy), we show that the iron-phosphorus granules and the magnetite crystals are likely formed through separate cellular processes. Analysis of the cellular ultrastructure of RS-1 using cryo-ultramicrotomy, cryo-electron tomography, and tomography of ultrathin sections reveals that the magnetite crystals are not surrounded by membranes but that the iron-phosphorus granules are surrounded by membranous compartments. The varied cellular paths for the formation of these two minerals lead us to suggest that the iron-phosphorus granules constitute a distinct bacterial organelle. 相似文献