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71.
Amino acid substitutions in GyrA affect quinolone susceptibility in Salmonella typhimurium 下载免费PDF全文
Siriporn Kongsoi Ruchirada Changkwanyeun Kazumasa Yokoyama Chie Nakajima Kanjana Changkaew Orasa Suthienkul Yasuhiko Suzuki 《Drug testing and analysis》2016,8(10):1065-1070
The prevalence of quinolone‐resistant Salmonella has become a public health concern. Amino acid substitutions have generally been found within the quinolone resistance‐determining region in subunit A of DNA gyrase (GyrA) of Salmonella Typhimurium. However, direct evidence of the contribution of these substitutions to quinolone resistance remains to be shown. To investigate the significance of amino acid substitutions in S. Typhimurium GyrA to quinolone resistance, we expressed recombinant wild‐type (WT) and five mutant DNA gyrases in Escherichia coli and characterized them in vitro. WT and mutant DNA gyrases were reconstituted in vitro by mixing recombinant subunits A and B of DNA gyrase. The correlation between the amino acid substitutions and resistance to quinolones ciprofloxacin, levofloxacin, nalidixic acid, and sitafloxacin was assessed by quinolone‐inhibited supercoiling assays. All mutant DNA gyrases showed reduced susceptibility to all quinolones when compared with WT DNA gyrases. DNA gyrase with a double amino acid substitution in GyrA, serine to phenylalanine at codon 83 and aspartic acid to asparagine at 87 (GyrA‐S83F‐D87N), exhibited the lowest quinolone susceptibility amongst all mutant DNA gyrases. The effectiveness of sitafloxacin was shown by the low inhibitory concentration required for mutant DNA gyrases, including the DNA gyrase with GyrA‐S83F‐D87N. We suggest sitafloxacin as a candidate drug for the treatment of salmonellosis caused by ciprofloxacin‐resistant S. Typhimurium. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
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Siriporn Chongkae Joshua D. Nosanchuk Kritsada Pruksaphon Angkana Laliam Soraya Pornsuwan Sirida Youngchim 《Journal of basic microbiology》2019,59(11):1092-1104
Melanins are one of the great natural pigments produced by a wide variety of fungal species that promote fitness and cell survival in diverse hostile environments, including during mammalian infection. In this study, we sought to demonstrate the production of melanin in the conidia and hyphae of saprophytic fungi, including dematiaceous and hyaline fungi. We showed that a melanin‐specific monoclonal antibody (MAb) avidly labeled the cell walls of hyphae and conidia, consistent with the presence of melanin in these structures, in 14 diverse fungal species. The conidia of saprophytic fungi were treated with proteolytic enzymes, denaturant, and concentrated hot acid to yield dark particles, which were shown to be stable free radicals, consistent with their identification as melanins. Samples obtained from patients with fungal keratitis due to Fusarium falciforme, Aspergillus fumigatus, Aspergillus flavus, Curvularia lunata, Exserohilum rostratum, or Fonsecaea pedrosoi were found to be intensely labeled by the melanin‐specific MAb at the fungal hyphal cell walls. These results support the hypothesis that melanin is a common component that promotes survival under harsh conditions and facilitates fungal virulence. Increased understanding of the processes of melanization and the development of methods to interfere with pigment formation may lead to novel approaches to combat these complex pathogens that are associated with high rates of morbidity and mortality. 相似文献
74.
Tunapong Wannipa Apaijai Nattayaporn Yasom Sakawdaurn Tanajak Pongpan Wanchai Keerati Chunchai Titikorn Kerdphoo Sasiwan Eaimworawuthikul Sathima Thiennimitr Parameth Pongchaidecha Anchalee Lungkaphin Anusorn Pratchayasakul Wasana Chattipakorn Siriporn C. Chattipakorn Nipon 《European journal of nutrition》2018,57(6):2091-2104
European Journal of Nutrition - In metabolic syndrome, the composition of gut microbiota has been disrupted, and is associated with left ventricular (LV) dysfunction. Several types of prebiotics,... 相似文献
75.
Liao Suchan Luo Ying Chunchai Titikorn Singhanat Kodchanan Arunsak Busarin Benjanuwattra Juthipong Apaijai Nattayaporn Chattipakorn Nipon Chattipakorn Siriporn C. 《Inflammation research》2022,71(7-8):861-872
Inflammation Research - Microglial hyperactivation and apoptosis were observed following myocardial infarction and ischemia reperfusion (I/R) injury. This study aimed to test the hypothesis that... 相似文献
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Uppanan P Channasanon S Veeranondh S Tanodekaew S 《Journal of biomedical materials research. Part A》2011,98(2):185-191
The dressing prepared from GTMAC modified chitin-PAA was introduced with the aim of facilitating wound healing, particularly those effectively absorbing exudates, maintaining a moist wound environment and controlling bacterial proliferation. Chitin was chemically modified with acrylic acid to encourage a moist wound healing environment. The highly water-absorbable resulting product (chitin-PAA) was further reacted with glycidyltrimethylammonium chloride (GTMAC) to impart antibacterial activities. The final product, chitin-PAA-GTMAC was characterized by the techniques of Fourier Transform Infrared (FTIR), solid state (15) N NMR, and elemental analysis. Their cytotoxicity and antibacterial activities against S. epidermidis and E. coli were evaluated which found increasing effects in those properties with increasing degree substitution of GTMAC. All materials also showed good blood-clotting ability. The collagen gel contraction assay was used to analyze the behavior of fibroblasts after contact with the gels. The extent of the gel contraction as well as the examination of the secreted interleukin-8 (IL-8) and transforming growth factor-β1 (TGF-β1) were investigated. The results showed that chitin-PAA modified with GTMAC could stimulate the production of IL-8, but TGF-β1. Fibroblasts presented normal spreading and formation of cellular processes in the collagen gels with all of the modifications. Furthermore, all modified gels except for the highest GTMAC content gel [chitin-PAA-GTMAC (1:20)] were found a greater extent in gel contraction than the unmodified chitin-PAA. It suggested the promoting effect of GTMAC on cell proliferation if the GTMAC content in the gel was not too high, that is, the mole ratio of glucosamine to GTMAC of the gel should not greater than 1:10. 相似文献
78.
Kamran A. Ahmed Jessica M. Freilich Yazan Abuodeh Nicholas Figura Neha Patel Siriporn Sarangkasiri Prakash Chinnaiyan Hsiang-Hsuan Michael Yu Arnold B. Etame Nikhil G. Rao 《Journal of neuro-oncology》2014,118(1):179-186
Following surgical resection for brain metastases, fractionated stereotactic radiotherapy (FSRT) has been used as an alternative to single dose treatment for large cavities and to reduce risks of late toxicity. The purpose of this study was to evaluate the outcomes of patients treated with FSRT to the post-operative bed for both radioresistant and radiosensitive brain metastases. Between December 2009 and May 2013 a total of 65 patients with newly diagnosed brain metastases were treated with resection followed by FSRT. Patients were treated to a total dose of 20–30 Gy in five fractions. Median planning target volume (PTV) was 16.88 cm3 (range 4.87–128.43 cm3). The median follow-up for all patients was 8.5 months (range 1.1–28.6 months) with a median of 12.9 months for living patients. One and two year Kaplan–Meier estimates of local control were 87.0 and 70.0 %, respectively. Local control at 1 year was 85.6 and 88.0 % for radioresistant and radiosensitive tumors, respectively (p = 0.44). A PTV ≥17 cm3, was associated with local failure, HR 8.63 ((1.44–164.78); p = 0.02). One and two year distant control rates were 50.9 and 46.2 %, respectively with six patients (9.2 %) experiencing leptomeningeal disease. OS rates at 1 and 2 years were 65.2 and 47.5 %, respectively. Survival was significantly associated with recursive partitioning analysis class (p = 0.001) and graded prognostic assessment score (p = 0.005). One case of radionecrosis was noted on follow-up imaging. FSRT in five fractions offers excellent local control in both radiosensitive and radioresistant tumors with minimal toxicity. 相似文献
79.
Vatanavicharn N Champattanachai V Liammongkolkul S Sawangareetrakul P Keeratichamroen S Ketudat Cairns JR Srisomsap C Sathienkijkanchai A Shotelersuk V Kamolsilp M Wattanasirichaigoon D Svasti J Wasant P 《Molecular genetics and metabolism》2012,106(4):424-429
Isolated methylmalonic acidemia (MMA) is a genetically heterogeneous organic acid disorder caused by either deficiency of the enzyme methylmalonyl-CoA mutase (MCM), or a defect in the biosynthesis of its cofactor, adenosyl-cobalamin (AdoCbl). Herein, we report and review the genotypes and phenotypes of 14 Thai patients with isolated MMA. Between 1997 and 2011, we identified 6 mut patients, 2 cblA patients, and 6 cblB patients. The mut and cblB patients had relatively severe phenotypes compared to relatively mild phenotypes of the cblA patients. The MUT and MMAB genotypes were also correlated to the severity of the phenotypes. Three mutations in the MUT gene: c.788G>T (p.G263V), c.809_812dupGGGC (p.D272Gfs*2), and c.1426C>T (p.Q476*); one mutation in the MMAA gene: c.292A>G (p.R98G); and three mutations in the MMAB gene: c.682delG (p.A228Pfs*2), c.435delC (p.F145Lfs*69), and c.585-1G>A, have not been previously reported. RT-PCR analysis of a common intron 6 polymorphism (c.520-159C>T) of the MMAB gene revealed that it correlates to deep intronic exonization leading to premature termination of the open reading frame. This could decrease the ATP:cobalamin adenosyltransferase (ATR) activity resulting in abnormal phenotypes if found in a compound heterozygous state with a null mutation. We confirm the genotype-phenotype correlation of isolated MMA in the study population, and identified a new molecular basis of the cblB disorder. 相似文献
80.
Christopher Adlbrecht Elmar Aigner Juan M. Bellón Izolde Bouloukaki Alberto Bouzas‐Mosquera Alexandre J. F. Carrilho Kuo‐Chu Chang Nipon Chattipakorn Siriporn C. Chattipakorn Yi‐Jen Chen Yuan‐Chiang Chung Roshan Colah Christian Datz Jens B. Frøkjær Shunji Fujimori Panagiota Georgiadou Cintia M. Grion Chih‐Ping Hsu Martin Hülsmann Ming‐Jui Hung Ming‐Yow Hung Efstathios K. Iliodromitis Irene M. Lang Ting‐ I. Lee Winfried März Sona B. Nair Gemma Pascual Jesús Peteiro Choitsu Sakamoto Atsushi Satomura Sophia E. Schiza Peter Stärkel Tatjana Stojakovic David L. Vesely Darren L. Walters Yusuf Yilmaz 《European journal of clinical investigation》2012,42(11):1149-1164