全文获取类型
收费全文 | 119篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 15篇 |
基础医学 | 14篇 |
口腔科学 | 2篇 |
临床医学 | 6篇 |
内科学 | 36篇 |
皮肤病学 | 2篇 |
神经病学 | 8篇 |
外科学 | 19篇 |
综合类 | 4篇 |
预防医学 | 2篇 |
药学 | 8篇 |
肿瘤学 | 6篇 |
出版年
2015年 | 4篇 |
2014年 | 1篇 |
2011年 | 1篇 |
2010年 | 2篇 |
2009年 | 3篇 |
2008年 | 1篇 |
2007年 | 2篇 |
2006年 | 5篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 1篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 11篇 |
1997年 | 14篇 |
1996年 | 11篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 1篇 |
1992年 | 8篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1984年 | 2篇 |
1983年 | 5篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1957年 | 1篇 |
1956年 | 3篇 |
1955年 | 1篇 |
排序方式: 共有122条查询结果,搜索用时 203 毫秒
71.
目的揭示CBF1在骨髓基质细胞Kusa-A1成骨分化过程中的作用。方法采用基因转染法建立CBF1稳定过表达细胞系Kusa-A1/CBF1,检测其成骨活性。指标包括细胞钙沉积能力、碱性磷酸酶活性、体外钙化结节(CN)形成、实时PCR测定细胞骨钙素(OC)和骨桥蛋白(OPN)基因表达、蛋白印迹检测细胞RANKL蛋白。最后用报告基因法检测CBF1对HES1启动子活性的影响。结果经RT-PCR和Western blot鉴定,Kusa-A1/CBF1细胞建立成功。与对照细胞系Kusa-A1/host相比,Kusa-A1/CBF1细胞的钙沉积能力、CN形成能力均显著提高;Kusa-A1/CBF1细胞OC和OPN基因表达和RANKL蛋白水平明显高于对照细胞。瞬时转染Notch的细胞内结构域NICD促进HES1的启动子活性,这一作用被CBF1强烈抑制。结论CBF1可以促进Kusa-A1的成骨分化,该作用至少部分是通过影响Notch信号实现的。 相似文献
72.
73.
KAZUHIKO FUJITA YUKIO ITOH RYO WADA KEIJI NAGAO MAKOTO FUJIME 《International journal of urology》2006,13(4):473-475
A burned-out testicular tumor with lung and lymph node metastases presented high serum levels of DU-PAN-2. However, the serum levels of CA19-9, alpha-fetoprotein, beta-human chorionic gonadotropin, and sialyl Lewis X were normal. A biopsy of the cervical lymph nodes revealed embryonal carcinoma. Four courses of chemotherapy, including cisplatin, etoposide, and bleomycin, normalized the level of DU-PAN-2, and the metastatic lesions disappeared. This is the second reported case of an embryonal carcinoma producing DU-PAN-2. The secretor and Lewis gene typings, which affect the normal serum ranges of CA19-9 and DU-PAN-2, were determined. 相似文献
74.
Core temperature pattern and self-rated lifestyle 总被引:1,自引:1,他引:0
KAZUHIKO FUKUDA PhD KANEYOSHI ISHHARA TOMOKA TAKEUCHI Ph.D. TOMOKA TAKEUCHI MAKI INUGAMI 《Psychiatry and clinical neurosciences》1998,52(2):243-245
Abstract We investigated the relationship between a subject's self-rated lifestyle and their pattern of nocturnal rectal temperature. Fifty-five students participated in the study. Among several significant findings, irregularity and eveningness component of lifestyle (irregular and delayed sleep phase) showed a significant negative correlation with temperature at the morning rising time. Examination of the nocturnal temperature pattern revealed that rectal temperature stayed at its lowest level in the early morning hours in the subjects with irregular and eveningness-like lifestyles. 相似文献
75.
KAZUHIKO ORIKASA TAKASHIGE NAMIMA SHOZO OTA MOTOHIKO MIURA HIKARU HAMA NORIKO KIMURA TETSUTARO OHNUMA 《International journal of urology》2003,10(11):622-624
A 71-year-old man with a history of rheumatoid arthritis was treated with intravesical bacillus Calmette-Guérin (BCG) instillation of 80 mg once-a-week for carcinoma in situ. He developed low-grade fever followed by dyspnea and severe hypoxemia. Radiological and laboratory studies revealed bilateral diffuse reticulonodular infiltrates and hypereosinophilia. A lymphocyte stimulation test for BCG was strongly positive. From these findings, a pulmonary hypersensitivity reaction to immunotherapy was suspected, and therefore, methylprednisolone (500 mg per day) was started. After that, the fever and dyspnea disappeared, the hypereosinophilia was normalised and chest radiography results were clear. The present case is the first reported case of eosinophilic pneumonia following intravesical BCG therapy. 相似文献
76.
TOSHIHIDE SATO KAZUFUMI OKAMOTO ICHIRO KUKITA KOICHI KIKUTA MASAMICHI HAMAGUCHI KAZUHIKO SHIIHARA YOSHIHIRO SHIBATA 《Pediatrics international》1997,39(2):172-175
During nitric oxide (NO) inhalation therapy, toxicity may be produced by the reactive metabolite nitrogen dioxide (NO2). The purpose of the present study was to determine the NO2 concentration in a NO inhalation system used for respiratory failure in children at relatively low concentrations of NO (< 20 ppm). The production of NO2 in the NO inhalation system using the Servo Ventilator 900C connected to the test lung under each of 30 combinations of NO concentrations (0, 4, 8, 12, 16, and 19 ppm) and inspired oxygen (O2) concentrations (21, 40, 60, 80, and 100%). Pressure controlled ventilation was used with a respiratory rate of 20 breaths/min. NO and NO2 measurements were obtained on the inspiratory side of the Y-piece connected to the test lung. At a given NO level, increases in the concentration of inspired O2 resulted in increases in the concentration of NO2 produced, as did increases in the amount of NO at a given concentration of O2. The mean NO2 concentration at the inspiratory site of the Y-piece did not exceed 0.05 ppm (the limit of NO2 as an outdoor air pollutant in the United States) when the NO concentration did not exceed 8 ppm, regardless of the O2 concentration. NO inhalation therapy for children with severe respiratory failure using the Servo Ventilator 900C can be performed safely when the concentration of NO does not exceed 8 ppm. 相似文献
77.
KIYOHIKO KURAI SHIRO IINO KAZUHIKO KOIKE KEIJI MITAMURA YASUO ENDO HIROSHI OKA 《Journal of gastroenterology and hepatology》1988,3(2):143-152
In the present study, aiming at the evaluation of the effects of antiviral therapy on hepatitis B e antigen (HBeAg) levels, serum HBeAg concentration was determined serially by a quantitative assay in 44 patients with chronic hepatitis B who received a total of 73 courses of antiviral treatment. The posttreatment HBeAg levels were significantly lower than pretreatment baseline in all treated groups, not only at the end of treatment but also 6 months after the completion of therapy. The proportional decrease in treated patients was significantly greater than in 15 untreated controls. Six months after the completion of treatment, serum HBeAg became negative in seven out of 25 treatments with interferon, seven out of 28 with adenine arabinoside, and five out of 20 with adenine arabinoside 5'-monophosphate. All patients who became negative for HBeAg had normalization of alanine aminotransferase as well. A progressive decrease in HBeAg in patients treated with multiple courses of drug was observed, and the frequency of negative tests increased, paralleling the number of courses of treatment. These results suggest that antiviral therapy may alleviate inflammatory changes in the liver by promoting clearance of HBeAg from the serum, and may eventually shorten the natural course of hepatitis associated with chronic hepatitis B virus infection. 相似文献
78.
KAZUHIKO MATSUNO MARIBEL DIAZ-RICART ROBERT R. MONTGOMERY RICHARD H. ASTER G. A. JAMIESON NARENDRA N. TANDON 《British journal of haematology》1995,92(4):960-967
Monoclonal anti CD36 antibodies capable of inhibiting platelet adhesion to collagen have not previously been identified. We have now prepared two groups of monoclonal antibodies. One group was prepared using, as immunogen, highly purified (99+%) CD36 prepared by a denaturing procedure. These antibodies (Mo series) reacted strongly with CD36 on protein blots but did not immunoprecipitate native CD36 from platelet lysates nor inhibit platelet adhesion to collagen. The second group of monoclonal antibodies (131 series) was prepared using CD36 purified to >95% by a non-denaturing procedure. These antibodies reacted with control platelets, but not Naka -negative platelets which lack CD36, as measured by flow cytometry and by immunoprecipitation. Three monoclonal antibodies of this latter group (131.4, 131.5 and 131.7) inhibited platelet adhesion to collagen in static systems under Mg2+ -independent conditions but had little effect in the presence of Mg2+ . 131.4 and 131.7 also inhibited adhesion to collagen using citrated whole blood in a parallel plate flow chamber at physiological shear rates (800 s−1 ), whereas 131.5 was without effect. These are the first anti-CD36 monoclonal antibodies shown to be capable of inhibiting platelet adhesion to collagen and provide further evidence that CD36 plays a role in platelet–collagen interaction. 相似文献
79.
CASE REPORT: A patient who developed an amoebic liver abscess during treatment with interferon 总被引:1,自引:0,他引:1
TAKU MATSUO HARUHIDE SHINZAWA KAZUHIKO SUGAHARA HIDEKI MITSUHASHI HISAYOSHI WATANABE TAKASHI ABE SHIGERU OHNO MASAKI TERASHITA KOUJI SAITO TAKAFUMI SAITO HIROYUKI MISAWA HITOSHI TOGASHI TSUNEO TAKAHASHI 《Journal of gastroenterology and hepatology》1998,13(10):1068-1071
A 65-year-old female received recombinant interferon (IFN) α-2b daily for the treatment of chronic hepatitis C. Fever (39°C or higher) developed 14 days after the start of administration. Abdominal computed tomography suggested multiple liver abscesses, which had not been detected before IFN administration. An autopsy revealed an amoebic liver abscess. A subclinical infection of Entamoeba histolytica in this case developed into amoebic liver abscess during IFN administration. 相似文献
80.
KAZUHIKO SAITOH NOBORU TSUKAMOTO HIROMASA MITSUHATA YOSHIHIRO HIRABAYASHI & REIJU SHIMIZU 《Paediatric anaesthesia》1996,6(6):495-497
A three-year-old girl who underwent an operation for adrenal neuroblastoma was anaesthetized with sevoflurane and epidural analgesia. In the immediate recovery period she had convulsions. The convulsions were successfully treated with thiopentone and sevoflurane, there were no neurological sequelae. The convulsions were considered to be a manifestation of mepivacaine toxicity because of a high plasma mepivacaine concentration. Complications of paediatric regional analgesia and manifestations of mepivacaine toxicity under sevoflurane anaesthesia are discussed. 相似文献