牛磺酸预防大鼠高血压发生的作用及其机理的初探

目的探讨ATⅡ含量和ACE活性是否与动物年龄的增长存在着相关性及牛磺酸(Taurine)对高血压的预防作用机理。方法对不同周龄大鼠和补充不同水平牛磺酸和β丙氨酸(牛磺酸转运抑制剂)大鼠血液中血管紧张素Ⅱ(angiotensinⅡ,ATⅡ)含量和血管紧张素转换酶(angiotensinconvertingenzyme,ACE)活性进行比较。结果随着大鼠年龄的增长血液中ATⅡ含量随之增高(p<0.05),ACE活性也随之增加(p<0.05),补充牛磺酸后使血液中ATⅡ含量、ACE活性降低(p<0.05)。结论给动物补充牛磺酸对高血压的产生具有预防作用。     

多孔钽棒改善股骨颈骨折术后股骨头缺血性坏死患者的髋关节功能：随机对照临床试验方案

BACKGROUND:After femoral neck fracture, avascular necrosis of the femoral head occurs because of insufficient blood supply to the femoral head, resulting in increased difficulty in treatment, and greatly influencing the recovery of hip joint function. Core decompression and bone grafting is a common treatment method of avascular necrosis of the femoral head. A porous tantalum rod is a bone trabecula-like metal implant that is used to support weight-bearing area of necrotic bone, prevent further collapse of the necrotic area, and thereby exhibits favorable effects in the early treatment of avascular necrosis of the femoral head. Few randomized controlled studies are reported on porous tantalum rod implantation for treatment of avascular necrosis of the femoral head after femoral neck fracture surgery. OBJECTIVE: To investigate whether porous tantalum rod implantation can improve the hip joint function of patients with avascular necrosis of the femoral head subjected to femoral neck fracture surgery. METHODS: This is a prospective, single-center, randomized, controlled, open trial, which will be performed in the Department of Orthopedic Trauma, Qinghai University Affiliated Hospital, China. A total of 100 patients with avascular necrosis of the femoral head subjected to femoral neck fracture surgery will be randomly assigned to undergo core decompression and porous tantalum rod implantation (experimental group, n=50) or only core decompression (control group, n=50). All patients will be followed up for 1 year. Primary outcome measure is the percentage of patients whose hip joint function is graded as excellent as per Harris scores 12 months after surgery in total patient number in each group, which will be used to evaluate the recovery of hip joint function. Secondary outcome measures include (1) the percentage of patients with excellent hip joint function 1 and 6 months after surgery in total patient number in each group; (2) Visual Analogue Scale spine score prior to and 1, 6 and 12 months after surgery, which will be used to evaluate the severity of pain; (3) the percentage of patients presenting with femoral head collapse, prosthesis loosening and peri-prosthesis infection 6 and 12 months after surgery, which will be used to evaluate the biocompatibility of biomaterial with host; and (4) incidence of complications 6 and 12 months after surgery, which will be used to evaluate the safety of porous tantalum rod implantation. This study protocol has been approved by the Medical Ethics Committee of Qinghai University Affiliated Hospital of China (approval number: QHY1016B) and will be performed in accordance with the guidelines of the Declaration of Helsinki, formulated by the World Medical Association. Signed informed consent regarding trial procedure and treatment will be obtained from each patient.  DISCUSSION: This study is powered to validate the biocompatibility and therapeutic effects of porous tantalum rods in the treatment of avascular necrosis of the femoral head after femoral neck fracture surgery, which hopes to provide a novel persuasive surgical treatment method for this disease.     

腰脊神经后支的解剖在曼格磁贴外敷治疗腰痛定点的应用

目的探讨曼格磁贴治疗腰痛的效果。方法结合腰脊神经支的解剖,进行曼格磁贴的定点治疗腰痛。结果在治疗的90例患者中,总有效率达100%,总显效率达81.1%。结论曼格磁贴外敷治疗脊神经后支性腰痛,安全有效,无副作用。     

胰岛素样生长因子Ⅱ在大鼠骨骼肌运动性损伤再生中的作用

目的：通过观察大鼠骨骼肌运动损伤后具有修复作用的卫星细胞的增殖变化，探讨施加胰岛素样生长因子Ⅱ对肌肉再生的修复作用。方法：选择2个月龄SD大鼠40只。随机分为两组，实验组20只，对照组20只。将两组大鼠建立7d多次力竭跑台训练致骨骼肌损伤的动物模型，即每天力竭1次，连续7d。首次力竭后第1，3，5天实验组于右侧腓肠肌中部的肌膜下分别注射1mL（300ng）外源性胰岛素样生长因子Ⅱ；对照组注射1mL生理盐水。两组分别在第1次力竭后的第7，9，11，17，24，30天各处死大鼠3只，对两组大鼠骨骼肌行苏木精一伊红染色，以光镜和电镜超微结构观察增殖细胞核抗原表达（增殖细胞核抗原以细胞核出现棕黄色颗粒，增殖细胞核抗原指数以随机记数50个高倍视野下共计500个细胞百分率表示），并进行X^2检验。结果：①两组大鼠腓肠肌光镜观察结果：第1次力竭后的第7。11，17天实验组卫星细胞明显增多；对照组胶原纤维增多；24d实验组肌纤维塑形良好，对照组可见瘢痕愈合。②两组大鼠腓肠肌超微结构电镜观察结果：实验组线粒体形态较正常，糖原颗粒增多，可见新生的肌纤维；对照组可见肌浆网扩张，线粒体肿胀，还有凋亡细胞出现。③两组大鼠腓肠肌免疫组织化学染色观察增殖细胞核抗原的表达：实验组明显高于对照组（X^2=-9．023，P〈0．05）。结论：胰岛素样生长因子Ⅱ可以促进大鼠运动性骨骼肌损伤后的卫星细胞增殖，并减少瘢痕愈合，表明其对大鼠骨骼肌运动性损伤有加快损伤后修复和促进再生的作用。     

应用缝匠肌治疗伸膝功能障碍

Theusualtreatmentofdislocationorsubluxationofpatella，quadricepsfemorisparalysisafterpoliomyelitisaswellasweakex－tensorofkneeafterquadriceps－plastyonextensionstiffnessofkneeandafterpatellectomyresultedfromcomminutedfractureortuber－culosisofpatellawasfarfromsatisfied．Freeingthedistalone－thirdpartofsartoriusmusclewithoutcuttingitsinsertionfortreatmentoftheoutwardsdisplacementofpatellaandfreeingthedistaltwo－thirdspartofsartoriusmusclewithoutcuttingitsinsertionfortreatmenttheweakextensorofkne…     

骨细胞MLO-Y4在不同强度静磁场下的生物学效应

目的研究骨样细胞系MLO-Y4在不同强度静磁场下的生物学效应。方法 MLO-Y4细胞接受场强为500nT亚磁场、0.2T中强磁场及16T强磁场作用后,CCK8法检测细胞活性,HE染色分析细胞形态变化,实时定量PCR方法检测骨细胞功能相关基因的表达,ELISA或化学试剂盒检测骨细胞可溶性细胞因子的分泌,收集处理后的条件培养基诱导成骨细胞矿化及破骨细胞形成。结果与对照组相比,亚磁场对骨细胞活性和形态无影响,促进了RANKL而抑制了Cx43的基因表达,促进了细胞因子M-CSF的分泌,其条件培养基对成骨细胞矿化无影响,但促进了破骨细胞形成。中强磁场对细胞活性无影响,增加了骨细胞突触数目,其条件培养基对成骨和破骨细胞分化都无明显影响。16T强磁场促进了骨细胞活性及细胞突触数目,抑制了RANKL而促进iNOS和Cx43基因表达,促进ALP和NO而抑制M-CSF分泌,其条件培养基对成骨细胞矿化无影响,但抑制了破骨细胞形成。结论骨细胞MLO-Y4在500nT亚磁场下表现为负向的生物学效应,0.2T中强磁场下无明显生物学行为变化,而在16T强磁场下表现为积极的生物学效应。     

小腿中段离断再植后重建足跖屈背伸功能

目的 探讨小腿中段离断再植后足跖屈背伸功能重建的方法。方法 常规断肢再植,术中将离断肢远段失神经支配的肌组织切除,只保留肌腱及肌腱膜,切取髂胫束条桥接远端背伸肌腱和近端肌腹,同时和前移缝匠肌、股二头肌长头缝接,加强足背伸肌力;桥接远端跖屈肌腱和近端肌腹,同时和游离的半腱肌、股薄肌缝接,加强足跖屈肌力。结果 治疗2例,分别随访2年8个月及2年5个月,足背伸、跖屈肌力达Ⅳ－Ⅵ^ ,踝关节活动度近正常。结论 应用髂胫束条桥接远断端的肌腱和近断端的正常肌组织,同时利用缝匠肌、股二头肌、半腱肌、股薄肌加强瞳的背伸、跖屈肌力,是一种有效的功能重建方法。