吗啡成瘾性大鼠脑内核团神经细胞[Ca 2+]i变化的研究

目的 研究吗啡成瘾对大鼠脑内与成瘾相关核团神经细胞[Ca 2 ]i的影响.方法 将50只SD大鼠随机分成吗啡成瘾组和对照组,观察成瘾后的戒断症状;应用对Ca2 敏感的探针Fluo-3与Ca2 络合后被激光激发发出荧光的特性,利用激光共聚焦显微镜观察伏核、海马、内侧额前皮质神经细胞[Ca 2 ]i变化,并用图像分析软件进行处理.结果 吗啡成瘾大鼠脑内伏核、海马及内侧额前皮质神经细胞[Ca 2 ]i和对照组比较明显增高(P<0.01).结论 长期使用吗啡可明显增高伏核、海马和内侧额前皮质神经细胞[Ca 2 ]i.     

阿尔茨海默病脑白质葡萄糖代谢异常分析

目的探讨阿尔茨海默病(AD)脑白质葡萄糖代谢异常的意义。方法纳入33例符合美国精神障碍诊断与统计手册-第四版(DSM-IV)AD诊断标准的患者和健康对照20名,进行脑正电子发射断层成像(PET)检查。应用SPM软件对PET图像进行分析。结果①与健康对照相比,AD患者有广泛的白质葡萄糖代谢减低,减低较为明显的区域有右侧额叶皮质下白质、左侧额叶上中回皮质下白质(P<0.001);另外,AD患者左侧额叶内侧回皮质下白质、左侧枕叶楔回皮质下白质葡萄糖代谢增强(P<0.001);②与不伴有精神行为症状(BPS)的AD患者(16例)相比,伴有BPS的AD患者(17例)在左右枕叶中回、右侧枕叶楔回、右侧顶下小叶、左侧颞叶梭形回、左侧额叶内侧回等脑区的皮质下白质葡萄糖代谢增强(P<0.001);而左右额叶中央旁回、右侧额叶上回和中回、左侧颞叶上回等脑区的皮质下白质葡萄糖代谢减低(P<0.001)。结论AD有广泛的白质脑葡萄糖代谢异常,有无BPS的AD白质代谢异常不同。     

Effects of hypoxic preconditioning on the changes of expression of neuroglobin mRNA and labeled positive cells following cerebral ischemia in gerbils

INTRODUCTION Neuroglobin (NGB) is a recently discovered member of the vertebrate globin family [1], predominantly expressed in various brain regions of human and vertebrate. The expression of NGB was increased in case of ischemia, which may play a role in…     

结肠肝曲癌侵犯十二指肠的外科治疗

目的:探讨结肠肝曲癌侵犯十二指肠的外科治疗。方法:对1996年1月—2006年8月12例结肠肝曲癌侵犯十二指肠的临床资料进行回顾性分析。结果:结肠肝曲癌侵犯十二指肠程度分两种类型:Ⅰ型局部浸润型,Ⅱ型广泛浸润型或癌性穿孔并内瘘形成。本组12例均行手术治疗,分别采取姑息性手术2例,右半结肠连同十二指肠浸润灶局部切除9例,右半结肠连同十二指肠节段性切除1例．十二指肠缺损单纯修补6例,十二指肠、空肠Roux-en-Y吻合术4例。术后无肠瘘及十二指肠梗阻等并发症。结论:根据结肠肝曲癌侵犯十二指肠不同特点,采取积极合理的手术方式有助于改善病情或减少术后并发症。     

输尿管镜下气压弹道碎石治疗输尿管结石失败原因探讨(附36例报告)

目的:探讨输尿管镜下气压弹道碎石(URSL)治疗输尿管结石失败原因及防治措施.方法:回顾分析36例气压弹道碎石治疗输尿管结石手术失败的临床资料.结果:17例手术中结石移位进入肾盂,无法碎石;8例因输尿管走行迂曲或输尿管狭窄置镜未成功;5例因局部出血或引流不畅导致视野不清终止手术;6例因输尿管穿孔而改开放手术.结论:结石移位、输尿管走行迂曲或狭窄、术中视野不清、输尿管穿孔是手术失败的主要原因.合理选择病例,保持液适当的灌注速度和压力,保持视野的清晰和在直视下推进输尿管镜是手术成功的关键.     

P17 Pharmacology in the integrated course ＂Basic Medical Sciences＂ in Zhejiang University School of Medicine

According to traditional teaching mode, the courses in preclinical medicine including pharmacology are separately run. This mode causes a series of disadvantages including loose connection between knowledge in different disciplines and weak ability to bridge basic preclinical knowledge and clinical practice. In order to overcome the disadvantages and promote the teaching efficiency, we constructed a new integrated course-Course of Basic Medical Sciences, which includes 6 traditional courses, anatomy, histology and embryology, physiology, pathology, pathophysiology and pharmacology. We integrated these courses based on the human organ systems and according to the principle-＂ From macro to micro, From morphological to functional, From normal to abnormal and From disease to drug therapy＂ and published the series of textbook in 2004. The contents of pharmacology are taught just after pathology and pathophysiology in every organ system. In comparison with the traditional teaching mode, teachers of pharmacology need not spend a lot of time to review preceding knowledge of anatomy and histology, physiology, pathophysiology and pathology. This is helpful in saving time and improving the teaching efficiency.     

对大肠癌术后早期炎性肠梗阻诊治的探讨

目的：探讨大肠癌术后早期炎性肠梗阻的特点及治疗方法；方法：分析10例大肠癌术后出现早期炎性肠梗阻的临床表现并进行综合保守治疗；结果：10例患者保守治疗全部成功；结论：术后早期炎性肠梗阻是一种非细菌性炎性肠梗阻，以发生在术后早期，腹胀、呕吐明显，腹痛轻或无为主要临床特点，治疗应采用保守治疗。     

姜黄素对大鼠早期肝缺血再灌注损伤肺的保护作用

目的 探讨姜黄素对大鼠肝脏缺血再灌注早期损伤(再灌注1、3 h)肺的保护作用.方法 将大鼠随机分为假手术组(A组)、对照组(B组)和实验组(C组).通过检测再灌注早期肺组织病理学的改变及肺组织中SOD、CAT、MDA、MPO的含量来评价姜黄素对大鼠肝脏缺血再灌注损伤肺的保护作用.结果 姜黄素可减少大鼠肝缺血再灌注损伤肺间隔的水肿和肺泡中红细胞和白细胞的渗出.姜黄素提高了早期缺血再灌注后肺组织中SOD、CAT含量,降低了肺组织中MDA、MPO含量.结论 姜黄素通过抑制肺组织中的氧自由基的生成及中性粒细胞的浸润,从而在早期大鼠肝缺血再灌注损伤中起到了对肺的保护.