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排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
F. R. Lin X. M. Wang H. S. Hsu V. R. Mumaw I. Nakoneczna 《International journal of experimental pathology》1987,68(4):539-550
Highly susceptible inbred male C57BL/6 mice were infected with 2 X 10(7) virulent Salmonella typhimurium by intraperitoneal inoculation. Samples of the liver were removed 2 or 3 days post-infection for examination by electron microscopy. Rapid infiltration of polymorphs and macrophages was observed in the site of infection. Visual evidence is presented to confirm the destruction of salmonellae within these inflammatory phagocytes as previously reported. The proliferation of the pathogens occurred in the extracellular locations of sinusoids and early lesions, and within hepatocytes. 相似文献
992.
Acute immune complex mediated glomerulonephritis in a Chinese girl with Wiskott-Aldrich syndrome variant 总被引:1,自引:0,他引:1
This study presents a 12-year-old girl with Wiskott-Aldrich syndrome variant, who developed acute glomerulonephritis without history of transfer factor therapy and the efficacy of splenectomy for the control of the patient's thrombocytopenia. The patient presented with eczema, severe thrombocytopenia and immunodeficiency. The impaired immunity was featured by impaired delayed hypersensitivity and lymphoproliferative response to nonspecific mitogen, low serum IgM, low isohaemagglutinins, recurrent infections and high IgE. She developed hematuria about one month prior to admission. For her severe thrombocytopenia splenectomy was performed and proved to be effective. At the time of splenectomy, renal biopsy was done and showed proliferative glomerulonephritis with coarse granular deposition of immunoglobulins (IgA and IgM) and C3. Electron microscopy demonstrated granular electron-dense deposits in the glomerulus, indicating an immune complex glomerulonephritis. 相似文献
993.
Omeñaca C Turett G Yarrish R Astiz M Lin R Kislak JW Cadden J 《Journal of acquired immune deficiency syndromes (1999)》1999,22(2):155-160
To identify characteristics associated with mortality in HIV-infected patients with bacteremia, 88 bacteremic episodes in 80 HIV-infected patients were prospectively identified over a 5-month period and observed for 30 days. Demographic, clinical, laboratory, and radiologic data were collected. Mean and median age was 41 years. Most study subjects were homosexual men. Median CD4 count was 20 cells/mm3. Gram-positive organisms predominated (65%). The most common source of bacteremia was intravascular catheters (45%). Overall mortality was 30%. A history of malignancy, three or more opportunistic infections, shock, low hemoglobin, source of bacteremia other than an intravascular catheter, resistance to therapy, and a second bacteremic episode during the study period, were all found to be independent predictors of mortality. In this cohort of HIV-infected patients, most of whom were severely immunosuppressed, several factors were found to be significantly and independently associated with mortality. 相似文献
994.
Insulin stimulation of lactate accumulation in isolated human granulosa- luteal cells: a comparison between normal and polycystic ovaries 总被引:2,自引:1,他引:2
Polycystic ovary syndrome (PCOS) is often associated with hyperinsulinaemia
and peripheral insulin resistance. Whether the ovary is resistant to
insulin is a matter of controversy. The aim was therefore to study the
effect of insulin on lactate accumulation, an indicator of glucose
metabolism, in granulosa-luteal cells from women with PCOS and from women
with normal ovarian function. The cells were obtained from women undergoing
clinical in-vitro fertilization-embryo transfer, either from patients with
normal ovarian function and tubal or male infertility, or from women with
PCOS, with or without tubal factor. The patients were down-regulated with
buserelin and stimulated with urofollitrophin and human chorionic
gonadotrophin (HCG). Follicle aspiration was performed under ultrasound
guidance. Following oocyte recovery the granulosa-luteal cells were
isolated, washed and cultured (2-3 x 10(4) viable cells/well) in serum-free
Eagle's minimal essential medium for 48 h. After washing, the cells were
then cultured in medium containing HCG (0.1-10 IU/ml) or insulin (0.05-0.5
microg/ml) for 24-48 h. Lactate accumulation in the media and cellular
protein were analysed. Basal lactate accumulation did not differ in
granulosa-luteal cells obtained from normal or PCOS ovaries, and averaged
46 and 49 nmol/g protein/24 h, respectively. A significant stimulation
(40-60%) was obtained by HCG in both groups. Insulin caused a
dose-dependent increase in lactate in granulosa-luteal cells obtained from
normal ovaries (control: 45.5 +/- 6.3; insulin 0.5 microg/ml: 77 +/- 10
nmol/microg protein). Lactate accumulation in granulosa-luteal cells from
PCOS ovaries was not altered in the presence of insulin. These results
suggest that granulosa-luteal cell glucose metabolism is resistant to
insulin in PCOS.
相似文献
995.
996.
Jiang S Xin R Wu X Lin S Qian Y Ren D Tang G Wang D 《American journal of medical genetics》2000,96(3):289-292
Attention deficit hyperactivity disorder (ADHD) is a prevalent disorder in children. The etiology of this disease is not clear. Genetics studies have suggested the involvement of the dopamine DRD-4 receptor gene and dopamine transporter gene (DAT1). Clinical studies have shown that monoamine oxidase-B (MAO-B) inhibitors are effective in the treatment of ADHD. These findings suggest that monoamine oxidase (MAO) genes might be involved in the origin of ADHD. In the present work, the DXS7 locus of chromosome X, which is closely linked to MAO genes, was selected as a marker to study the possible association between ADHD and MAO genes in the Chinese population. Haplotype-based haplotype relative risk (HHRR) and the transmission disequilibrium test (TDT) methods were employed to analyze the association and the linkage disequilibrium, respectively. Significant association (X(2) = 15.86; 1 df; P < 0.001) and linkage (X(2) = 14.88; 1 df; P < 0.001) were detected between the 157-bp allele of the DXS7 locus and the DSM-III-R-diagnosed ADHD (N = 72) in trios composed of father, mother, and affected offspring. The data suggested that ADHD was associated and in linkage with DXS7 locus. 相似文献
997.
998.
Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge 总被引:4,自引:0,他引:4
Liu LY Jarjour NN Busse WW Kelly EA 《The Journal of allergy and clinical immunology》2003,112(3):556-562
BACKGROUND: The recruitment of circulating eosinophils to the lung is a characteristic feature of allergic airway inflammation. Chemokine receptors likely play a role in this complex process. However, reports of chemokine receptor expression on human eosinophils are conflicting. OBJECTIVE: The aim of this study was to determine whether the chemokine receptor profile of human eosinophils change when these cells are recruited to the airway after an antigen challenge and development of an allergic inflammatory response. METHODS: Blood and bronchoalveolar lavage (BAL) cells were obtained from 13 allergic subjects 48 hours after segmental bronchoprovocation with antigen. The CC chemokine receptor (CCR) 1 to 7, 9, and CXC chemokine receptor (CXCR) 1 to 4 were determined by flow cytometric analysis of whole blood and unseparated BAL cells. RESULTS: Compared with their circulating counterparts, airway eosinophils had decreased CCR3 and increased CCR4, CCR9, and CXCR3 expression on their cell surface. Furthermore, expression of CCR3, CCR4, and CXCR3 was significantly correlated with the percentage of eosinophils in BAL fluid at 48 hours. Eosinophils also expressed CXCR4, but this receptor did not change after antigen-induced recruitment to the airway. In contrast, the expression of CCR1, CCR2, CCR5, CCR6, CCR7, CXCR1, and CXCR2 remained undetectable on either blood or BAL eosinophils. CONCLUSIONS: Our data suggest that recruitment of eosinophils to the airway is associated with a modulation of their chemokine receptor profiles. These changes in chemokine receptors could be involved in determining eosinophil function and antigen-induced airway inflammation. 相似文献
999.
Kaposi's sarcoma-associated herpesvirus (KSHV) is the first human virus known to encode dihydrofolate reductase (DHFR), an enzyme required for nucleotide and methionine biosynthesis. We have studied the purified KSHV-DHFR enzyme in vitro and analyzed its expression in cultured B-cell lines derived from primary effusion lymphoma (PEL), an AIDS-associated malignancy. The amino acid sequence of KSHV-DHFR is most similar to human DHFR (hDHFR), but the viral enzyme contains an additional 23 amino acids at the carboxyl-terminus. The viral DHFR, overexpressed and purified from E. coli, was catalytically active in vitro. The K(m) of KSHV-DHFR for dihydrofolate (FH(2)) was 2.4 microM, which is significantly higher than the K(m) of recombinant hDHFR (rhDHFR) for FH(2) (390 nM). K(m) values for NADPH were similar for the two enzymes, about 1 microM. KSHV-DHFR was inhibited by folate antagonists such as methotrexate (K(i): 200 pM), aminopterin (K(i): 610 pM), pyrimethamine (K(i): 29 nM), trimethoprim (K(i): 2.3 microM), and piritrexim (K(i): 3.9 nM). In all cases, K(i) values for these folate antagonists were higher for KSHV-DHFR than for rhDHFR. The viral enzyme was expressed at levels two- to tenfold higher than hDHFR in PEL cell lines as an early lytic cycle gene. KSHV-DHFR mRNA and protein appeared from 6 to 24 h after chemical induction of the KSHV lytic cycle. Epitope-tagged KSHV-DHFR and rhDHFR both localized to the nucleus of transfected cells, while other KSHV nucleotide metabolism genes localized to the cytoplasm. DHFR activity was not essential for viral replication in cultured PEL cells. Since hDHFR was not detectable in peripheral blood mononuclear cells (PBMCs), KSHV-DHFR may function to provide increased DHFR activity in vivo in infected cells that have little or none of their own enzyme. 相似文献
1000.
白细胞图像自动识别系统的研究 总被引:2,自引:0,他引:2
我们所用的图像分割方法是在对图像距离变换的基础上,综合区域和边界方法,充分利用图像中包含的信息,实现白细胞图像的分割。根据细胞的形状、纹理、颜色等的特点选取并测定22个特征值,用统计分类的方法设计分类器。通过对560幅图像共831个细胞的测试表明,此系统的识别正确率为96%,经临床专家评估,本系统运用模式识别技术对外周血中的白细胞图像实现自动识别,具有较好的实用价值。 相似文献