促红细胞生成素对实验性肾性贫血的作用

促红细胞生成素(erythropoietin,EPO)是由肾细胞分泌的一种糖蛋白激素。从人胚肾细胞中诱导,经生物化学方法分离、提纯得到此品。本试验用5/6肾切除的方法造成大鼠慢性肾衰性(CRF)贫血,研究不同剂量EPO对CRF贫血的作用。结果表明EPO有显著的促进红细胞生成,改善CRF贫血状态,使其接近或达到正常水平,最佳剂量为1000 U/kg,并可预防实验性贫血,对正常鼠未见明显作用。     

Measuring depressive symptoms in the naturalistic primary-care setting

BACKGROUND: The majority of individuals with major depressive disorder are diagnosed and treated in the primary-care setting. A quantifiable critical objective in the management of depression is to achieve and sustain full symptomatic remission. The HAMD-7 is a depression metric validated in both tertiary and primary-care settings. METHODS: Herein, we further characterise the psychometric properties of the HAMD-7 in depressed patients treated in primary-care settings. Several cut-scores were evaluated for maximum agreement; diagnostic efficacy statistics with the original HAMD-7 items were also evaluated. We compared performance of the HAMD-7 in primary care to a previously characterised tertiary sample. RESULTS: The depressive symptoms most frequently endorsed (>or=70%) and most sensitive to change during antidepressant treatment in depressed primary-care patients were depressed mood, guilt, work and activities, psychic and somatic anxiety and fatigue. LIMITATIONS: This is a post hoc analysis of a primary-care database; assumptions regarding the definition of symptomatic remission in depression affect interpretation. CONCLUSION: Measurement-based care with the HAMD-7 quantifies the severity of commonly reported depressive items and their responsivity to treatment. The HAMD-7, inclusive of the suicide item, is capable of tracking symptom progress, with a validated remission cut-score.     

Effects of sera from burn patients on human hepatocytic viscoelasticity

ＥｆｅｃｔｓｏｆｓｅｒａｆｒｏｍｂｕｒｎｐａｔｉｅｎｔｓｏｎｈｕｍａｎｈｅｐａｔｏｃｙｔｉｃｖｉｓｃｏｅｌａｓｔｉｃｉｔｙＷＡＮＧＸｉａｏＪｕｎ，ＬＵＯＸｉａｎｇＤｏｎｇ，ＬＵＯＱｉｎａｎｄＹＡＮＧＺｏｎｇＣｈｅｎｇＢｕｒｎＲｅｓｅａｒｃｈＩｎ...     

Abnormal expression of hepatoma specific gamma-glutamyl transferase and alteration of gamma-glutamyl transferase gene methylation status in patients with hepatocellular carcinoma

Abnormal expression of hepatoma specific gamma-glutamyl transferase and alteration of gamma-glutamyl transferase gene methylation status in patients with hepatocellular carcinoma@Jiang DR @Huang ZW @Lu JX @Tao QY @Yu JZ @Meng XY     

Distribution of C-reactive protein and its relation to arterial hypertension in a country representing a high-risk region for cardiovascular diseases

AIMS: The high-sensitivity C-reactive protein (hs-CRP) measurement is recommended by ESC/ESH Guidelines 2003 in patients with arterial hypertension. The aim of this study was to assess distribution of hs-CRP in Poland, a country representing a high-risk region for CVD. METHODS AND RESULTS: In 2002, the cross-sectional survey NATPOL PLUS was performed in a representative sample of adults in Poland aged 18-94 years. The questionnaire, anthropometrical and blood pressure measurements, glycaemia, lipidogram and hs-CRP were performed in 2333 respondents. The results of hs-CRP > 10 mg/l were excluded from analysis. Mean hs-CRP was 2.03+/-2.14 mg/l (2.13+/-2.12 mg/l in women vs 1.91+/-2.16 mg/l in men; p < 0.05). Hs-CRP > or = 1 mg/l was observed in 58% of women and in 52% of men (p < 0.05). In hypertensives (> or =140/90 mmHg or treatment: women 30% vs men 29%, ns.), hs-CRP > or = 1 mg/l was found in 74% of women, and in 63% of men. The transformed values of hs-CRP [-1/[hs-CRP](1/2)] were higher in hypertensive patients than in normotensives (-0.94 vs -1.05; p < 0.01). CONCLUSION: (i) The increased level of hs-CRP was found in more than half of adults in Poland. (ii) The hs-CRP is higher in hypertensive patients than in normotensives. (iii) In view of the given results, routine measurements of hs-CRP in hypertensive patients in a country like Poland should be reconsidered.     

10例急性重症病毒性心肌炎的临床诊治经验

目的　分析、总结急性重症病毒性心肌炎病人的发病特点、临床诊治经验。方法　收集我院 1999年 11月～ 2 0 0 3年 4月间住院治疗的 10例急性重症病毒性心肌炎病人。男性 5例 ,女性 5例 ,年龄 18-4 7( 3 6± 10 .7)岁 ,住院 12 -2 2 ( 17.5± 3 .2 )天 ,所有诊断均符合 1999年制定的关于急性病毒性心肌炎临床诊断参考标准[1 ] 。结果　所有病人心肌酶学持续性升高 ,心脏超声提示心肌收缩、舒张功能减低。本组病例均累及心脏传导系统 ,主要表现为房室和室内传导阻滞 ( 1支或 2支 ) ,均行临时起搏器植入术 ,其中 2例更换为永久起搏器。 4例酷似心梗样心电图表现的病人冠状动脉造影检查正常。 2例患者出现严重心力衰竭、1例出现心肌心包炎、1例出现严重脑部并发症。结论　急性重症病毒性心肌炎病情危重 ,发展迅速 ,典型的临床表现和心肌损伤的客观指标为该疾病的诊断提供依据 ,应用大剂量皮质激素和必要的、紧急的血流动力学支持等治疗可以明显地改善心功能 ,挽救生命。     

二羟丙氧甲基乌嘌呤抗单纯疱疹病毒Ⅰ型的实验研究

二羟丙氧甲基鸟嘌岭(DHPG),在组织培养中对单纯疱疹病毒Ⅰ型(HSV-1)KOS株抑制50%空斑形成的浓度(IC_(50))为0.1μg/ml。DHPG与环胞苷联合应用的抗HSV活性呈协同作用;DHPG分别与无环鸟苷、酞丁安合用有相加作用。0.1和0.05%DHPG溶液滴眼对家兔实验性浅层单纯疱疹病毒角膜炎有显著治疗作用,浓度降低至0.025和0.0l%无明显疗效。对实质层型单疱角膜炎,0.1%DHPG溶液滴眼治疗有效。