全文获取类型
收费全文 | 863篇 |
免费 | 87篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 8篇 |
妇产科学 | 20篇 |
基础医学 | 191篇 |
口腔科学 | 2篇 |
临床医学 | 94篇 |
内科学 | 198篇 |
皮肤病学 | 21篇 |
神经病学 | 99篇 |
特种医学 | 21篇 |
外科学 | 58篇 |
综合类 | 3篇 |
一般理论 | 1篇 |
预防医学 | 55篇 |
眼科学 | 28篇 |
药学 | 78篇 |
肿瘤学 | 70篇 |
出版年
2023年 | 9篇 |
2022年 | 8篇 |
2021年 | 45篇 |
2020年 | 17篇 |
2019年 | 36篇 |
2018年 | 24篇 |
2017年 | 22篇 |
2016年 | 23篇 |
2015年 | 30篇 |
2014年 | 40篇 |
2013年 | 47篇 |
2012年 | 73篇 |
2011年 | 72篇 |
2010年 | 30篇 |
2009年 | 32篇 |
2008年 | 68篇 |
2007年 | 76篇 |
2006年 | 72篇 |
2005年 | 58篇 |
2004年 | 52篇 |
2003年 | 55篇 |
2002年 | 23篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1930年 | 1篇 |
排序方式: 共有958条查询结果,搜索用时 15 毫秒
11.
12.
13.
Iryna Boiko Daniel Golparian Inna Krynytska Magnus Unemo 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2019,127(9):627-634
Sexually transmitted infections (STIs) remain major public health problems globally. Appropriate laboratory diagnosis of STIs is rare in Ukraine. We investigated the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) using the US FDA‐approved Aptima Combo 2 and Aptima TV assays and compared the results with the conventional routine diagnostic tests (CDTs) in Ukraine. Urogenital swabs from consecutive mostly symptomatic females (n = 296) and males (n = 159) were examined. The prevalences were as follows: 10% (n = 47) of TV, 5.3% (n = 24) of CT and 1.5% (n = 7) of NG. The specificity of some CDTs was high, for example, 100% for NG culture, TV IgG ELISA, CT IgM ELISA and CT microscopy, but lower for other CDTs, that is, from 44% to 99.8%. The sensitivity of all CDTs was suboptimal, that is, 71% (n = 5) for NG microscopy, 57% (n = 4) for NG culture, 53% (n = 8) for CT IgG ELISA, 33% (n = 1) for TV IgG ELISA, 28% (n = 13) for TV microscopy, 25% (n = 1) for CT IgA ELISA, 20% (n = 3) for CT IgM ELISA and 0% (n = 0) for CT microscopy. The prevalences of particularly TV and CT were high, but substantial also for NG, in Ternopil, Ukraine. The sensitivities of all CDTs were low, and widespread implementation of validated, quality‐assured and cost‐effective molecular diagnostic STI tests in Ukraine is imperative. 相似文献
14.
15.
16.
17.
18.
19.
Abnormal calcium signaling and sudden cardiac death associated with mutation of calsequestrin 总被引:1,自引:0,他引:1
Viatchenko-Karpinski S Terentyev D Györke I Terentyeva R Volpe P Priori SG Napolitano C Nori A Williams SC Györke S 《Circulation research》2004,94(4):471-477
Mutations in human cardiac calsequestrin (CASQ2), a high-capacity calcium-binding protein located in the sarcoplasmic reticulum (SR), have recently been linked to effort-induced ventricular arrhythmia and sudden death (catecholaminergic polymorphic ventricular tachycardia). However, the precise mechanisms through which these mutations affect SR function and lead to arrhythmia are presently unknown. In this study, we explored the effect of adenoviral-directed expression of a canine CASQ2 protein carrying the catecholaminergic polymorphic ventricular tachycardia-linked mutation D307H (CASQ2(D307H)) on Ca2+ signaling in adult rat myocytes. Total CASQ2 protein levels were consistently elevated approximately 4-fold in cells infected with adenoviruses expressing either wild-type CASQ2 (CASQ2(WT)) or CASQ2(D307H). Expression of CASQ2(D307H) reduced the Ca2+ storing capacity of the SR. In addition, the amplitude, duration, and rise time of macroscopic I(Ca)-induced Ca2+ transients and of spontaneous Ca2+ sparks were reduced significantly in myocytes expressing CASQ2(D307H). Myocytes expressing CASQ2(D307H) also displayed drastic disturbances of rhythmic oscillations in [Ca2+]i and membrane potential, with signs of delayed afterdepolarizations when undergoing periodic pacing and exposed to isoproterenol. Importantly, normal rhythmic activity was restored by loading the SR with the low-affinity Ca2+ buffer, citrate. Our data suggest that the arrhythmogenic CASQ2(D307H) mutation impairs SR Ca2+ storing and release functions and destabilizes the Ca2+-induced Ca2+ release mechanism by reducing the effective Ca2+ buffering inside the SR and/or by altering the responsiveness of the Ca2+ release channel complex to luminal Ca2+. These results establish at the cellular level the pathological link between CASQ2 mutations and the predisposition to adrenergically mediated arrhythmias observed in patients carrying CASQ2 defects. 相似文献