全文获取类型
收费全文 | 5782篇 |
免费 | 331篇 |
国内免费 | 24篇 |
专业分类
耳鼻咽喉 | 34篇 |
儿科学 | 142篇 |
妇产科学 | 105篇 |
基础医学 | 825篇 |
口腔科学 | 104篇 |
临床医学 | 348篇 |
内科学 | 1352篇 |
皮肤病学 | 51篇 |
神经病学 | 368篇 |
特种医学 | 172篇 |
外科学 | 1012篇 |
综合类 | 20篇 |
预防医学 | 210篇 |
眼科学 | 340篇 |
药学 | 398篇 |
中国医学 | 3篇 |
肿瘤学 | 653篇 |
出版年
2023年 | 28篇 |
2022年 | 28篇 |
2021年 | 86篇 |
2020年 | 54篇 |
2019年 | 89篇 |
2018年 | 134篇 |
2017年 | 93篇 |
2016年 | 114篇 |
2015年 | 107篇 |
2014年 | 149篇 |
2013年 | 190篇 |
2012年 | 283篇 |
2011年 | 286篇 |
2010年 | 168篇 |
2009年 | 158篇 |
2008年 | 236篇 |
2007年 | 270篇 |
2006年 | 268篇 |
2005年 | 285篇 |
2004年 | 291篇 |
2003年 | 255篇 |
2002年 | 247篇 |
2001年 | 170篇 |
2000年 | 175篇 |
1999年 | 158篇 |
1998年 | 82篇 |
1997年 | 51篇 |
1996年 | 63篇 |
1995年 | 56篇 |
1994年 | 48篇 |
1993年 | 45篇 |
1992年 | 147篇 |
1991年 | 132篇 |
1990年 | 112篇 |
1989年 | 140篇 |
1988年 | 130篇 |
1987年 | 114篇 |
1986年 | 104篇 |
1985年 | 84篇 |
1984年 | 56篇 |
1983年 | 51篇 |
1982年 | 27篇 |
1980年 | 25篇 |
1979年 | 36篇 |
1976年 | 23篇 |
1972年 | 27篇 |
1971年 | 25篇 |
1970年 | 25篇 |
1967年 | 23篇 |
1966年 | 28篇 |
排序方式: 共有6137条查询结果,搜索用时 62 毫秒
71.
Yokomizoi K Nakayama A Hokazono E Ninomiya A Miyake R Hiratsuka N Okuyama M Kato Y Kobayashi S Ito Y Shiba K 《Rinsho byori. The Japanese journal of clinical pathology》2005,53(12):1109-1115
In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients. 相似文献
72.
73.
A novel insertional mutation at exon VII of the myelin proteolipid protein gene in Pelizaeus -- Merzbacher disease 总被引:1,自引:0,他引:1
Kurosawa Kenjl; Iwakl Akiko; Miyake Sho-ta; Imaizuml Kiyoshi; Kuroki Yoshikazu; Fukumakl Yasuyuki 《Human molecular genetics》1993,2(12):2187-2189
Pelizaeus Merzbacher disease (PMD) is an X-linked neurologicaldisorder characterized by dysmyelination in the central nervoussystem (CNS). Recently mutations of the myelln proteollpid protein(PLP) gene which encodes both PLP and Its Isoform, DM-20 generatedby alternative spllcing, have been demonstrated In PMD patients.We analyzed the seven exons of the PLP gene of a Japanese boyaffected with PMD by direct sequencing and identified an Insertionevent In exon Vll of the PLP gene. This mutation was also presentIn his carrier mother, but was absent In ninety-five X chromosomesof normal Japanese. The frame-shift mutation leads to the productionof truncated PLP with altered carboxyl terminal amlno acid sequences,resulting In conslderable change of the structure of PLP andDM-20 necessary for functional purposes. This is the first reportof a mutation In exon Vll of the PLP gene associated with PMD. 相似文献
74.
Zeynep H. Coban‐Akdemir Wu‐Lin Charng Mahshid Azamian Ingrid S. Paine Jaya Punetha Christopher M. Grochowski Tomasz Gambin Santiago O. Valdes Bryan Cannon Gladys Zapata Patricia P. Hernandez Shalini Jhangiani Harsha Doddapaneni Jianhong Hu Fatima Boricha Donna M. Muzny Eric Boerwinkle Yaping Yang Richard A. Gibbs Jennifer E. Posey Xander H. T. Wehrens John W. Belmont Jeffrey J. Kim Christina Y. Miyake James R. Lupski Seema R. Lalani 《American journal of medical genetics. Part A》2020,182(6):1387-1399
75.
The plasminogen activation system reduces fibrosis in the lung by a hepatocyte growth factor-dependent mechanism 总被引:12,自引:0,他引:12 下载免费PDF全文
Hattori N Mizuno S Yoshida Y Chin K Mishima M Sisson TH Simon RH Nakamura T Miyake M 《The American journal of pathology》2004,164(3):1091-1098
Mice deficient in the plasminogen activator inhibitor-1 gene (PAI-1-/- mice) are relatively protected from developing pulmonary fibrosis from bleomycin administration. We hypothesized that one of the protective mechanisms may be the ability of the plasminogen system to enhance hepatocyte growth factor (HGF) effects, which have been reported to be anti-fibrotic in the lung. HGF is known to be sequestered in tissues by binding to extracellular matrix components. Following bleomycin administration, we found that HGF protein levels were higher in bronchoalveolar lavage fluid from PAI-1-/- mice compared to wild-type (PAI-1+/+) mice. This increase could be suppressed by administering tranexamic acid, which inhibits plasmin activity. Conversely, intratracheal instillation of urokinase into bleomycin-injured PAI-1+/+ mice to activate plasminogen caused a significant increase in HGF within bronchoalveolar lavage and caused less collagen accumulation in the lungs. Administration of an anti-HGF neutralizing antibody markedly increased collagen accumulation in the lungs of bleomycin-injured PAI-1-/- mice. These results support the hypothesis that increasing the availability of HGF, possibly by enhancing its release from extracellular matrix by a plasmin-dependent mechanism, is an important means by which activation of the plasminogen system can limit pulmonary fibrosis. 相似文献
76.
77.
Development of nested PCR based on the ViaB sequence to detect Salmonella typhi. 总被引:1,自引:3,他引:1 下载免费PDF全文
Y Hashimoto Y Itho Y Fujinaga A Q Khan F Sultana M Miyake K Hirose H Yamamoto T Ezaki 《Journal of clinical microbiology》1995,33(3):775-777
For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level. 相似文献
78.
Toshiaki Maruyama Ichiro Saito Sachiko Miyake Hiroshi Hashimoto Kazuto Sato Hideo Yagita Ko Okumura Nobuyuki Miyasaka 《European journal of immunology》1993,23(9):2059-2065
Synovial T cells play a crucial role in the pathogenesis of rheumatoid arthritis (RA) synovitis. We have quantitatively analyzed the T cell receptor (TcR) variable (V) region gene repertoire of freshly isolated synovial fluid (SF) T cells, comparing it with that of peripheral blood (PB) T cells in RA. The TcR V gene repertoire of PB and SF T cells in RA and osteoarthritis was heterogeneous. In contrast, Vail in SF was expressed to a greater degree in three of five RA patients, and increased levels of Vp6, 1-3 were found in the SF of four of six RA, compared with paired PB. Of note, Vβ6, 1–3 was universally used in four RA patients with a disease duration of less than 10 years, irrespective of their HLA-DR types. This was in contrast to two other RA patients, suffering for more than 20 years, who showed different Vα and Vβ usages. β-chain sequence analysis in RA patients with a preference for Vβ6, 1–3 has shown that a few clones dominated in SF, whereas polyclonality was observed in PB. These findings suggest oligoclonal expansion of T cells in response to specific antigen(s) in the SF of these patients with RA of relatively short duration. Concomitant use of two hydrophobic amino acids, leucine and valine, in the Dβ region was noticeable among the predominant SF clones. These two amino acids might directly contact a peptide specific for the induction of synovitis in RA patients. TcR-directed therapy may, therefore, be useful for the treatment of early RA synovitis. 相似文献
79.
Kudoh M Satoh H Kaimori M Hayashi K Sakurabayashi I 《Rinsho byori. The Japanese journal of clinical pathology》2003,51(5):419-424
A 10-year-old child was diagnosed as subacute necrotizing lymphadenitis. After a steroid hormone (predonine) administration for 17 days, he showed total cholesterol(TC) 420 mg/dl, triglyceride(TG) 839 mg/dl, and LDL-cholesterol 241 mg/dl. The hyperlipidemia seemed to be a side effect of the steroid at the onset. However, the lipoprotein fraction by the agarose gel and polyacrylamide gel (PAG) electrophoresis showed type III of the WHO classification, that is, presence of broad band as well as appearance of mid band, small dense-LDL and the disrupted type of LDL band. In addition, there were hyperlipidemia (high levels of the TC, TG, LDL-cholesterol) in 4 persons out of 6 family members, and LDL pattern of the PAG electrophoresis, 4 persons showed the nodular type. They have higher possibility of combined-type familial hyperlipiemia from the above results, and it seemed to be the case in which the hyperlipidemia was exacerbated by the steroid administration. 相似文献
80.
An unusual case of a 37-year-old female with xanthomatous bone tumor of the right molar area of the mandible was presented. The tumor was asymptomatic and found to be a well-demarcated intraosseous radiolucent lesion on radiographic examination. Histologically the tumor consisted of two cell types, fibroblastic and xanthomatous cells. There was no osteoid, bone or cartilage formation. However, numerous psammomatous calcified bodies were seen in the fibrous area. Ultrastructural study showed fibroblastic cells in different stages of proliferation as the basis of the tumor which transform itself into xanthomatous cells. From the clinicopathologic findings, our case was thought to be a xanthic variant of non-ossifying fibroma (so-called xanthofibroma) of the mandible. 相似文献