全文获取类型
收费全文 | 2151篇 |
免费 | 106篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 17篇 |
儿科学 | 64篇 |
妇产科学 | 11篇 |
基础医学 | 253篇 |
口腔科学 | 25篇 |
临床医学 | 91篇 |
内科学 | 591篇 |
皮肤病学 | 113篇 |
神经病学 | 219篇 |
特种医学 | 37篇 |
外科学 | 413篇 |
综合类 | 5篇 |
预防医学 | 56篇 |
眼科学 | 75篇 |
药学 | 74篇 |
中国医学 | 3篇 |
肿瘤学 | 219篇 |
出版年
2023年 | 9篇 |
2022年 | 6篇 |
2021年 | 40篇 |
2020年 | 17篇 |
2019年 | 25篇 |
2018年 | 26篇 |
2017年 | 21篇 |
2016年 | 32篇 |
2015年 | 32篇 |
2014年 | 46篇 |
2013年 | 46篇 |
2012年 | 85篇 |
2011年 | 92篇 |
2010年 | 68篇 |
2009年 | 45篇 |
2008年 | 97篇 |
2007年 | 101篇 |
2006年 | 110篇 |
2005年 | 110篇 |
2004年 | 104篇 |
2003年 | 142篇 |
2002年 | 113篇 |
2001年 | 112篇 |
2000年 | 91篇 |
1999年 | 84篇 |
1998年 | 32篇 |
1997年 | 23篇 |
1996年 | 22篇 |
1995年 | 19篇 |
1994年 | 15篇 |
1993年 | 7篇 |
1992年 | 55篇 |
1991年 | 50篇 |
1990年 | 39篇 |
1989年 | 58篇 |
1988年 | 60篇 |
1987年 | 41篇 |
1986年 | 41篇 |
1985年 | 28篇 |
1984年 | 15篇 |
1983年 | 18篇 |
1982年 | 4篇 |
1981年 | 10篇 |
1980年 | 4篇 |
1979年 | 23篇 |
1978年 | 10篇 |
1977年 | 10篇 |
1970年 | 5篇 |
1969年 | 4篇 |
1968年 | 4篇 |
排序方式: 共有2266条查询结果,搜索用时 62 毫秒
61.
Hara Y Komatsu J Hiasa G Ohtsuka T Suzuki M Shigematsu Y Ohkura T Hamada M Hiwada K 《Angiology》2000,51(9):781-785
A 25-year-old man with low cardiac risks underwent coronary arteriography because of chest pain at rest. His only risk factors for coronary artery disease was smoking. Both right and left coronary arteries were ectatic and the left anterior descending artery was obstructed. In the acetylcholine provocation test, the left circumflex coronary artery showed severe constriction. Thus, this patient was diagnosed as having a combination of variant angina and occlusive coronary artery disease. 相似文献
62.
A 13 year old boy is described with hypereosinophilia associated with Kimura's disease, who showed repeated life threatening syncopal attacks during daily activities or at rest. Coronary arteriography demonstrated small aneurysms with irregular vessel walls of both coronary arteries, and the absence of organic stenotic lesions. Infusion of a minimal dose of ergonovine into the right coronary artery induced severe spasm of the vessel. Ventricular fibrillation recurred even after administration of nifedipine and isosorbide was started, but was completely inhibited by prednisolone.
Keywords: coronary vasospasm; hypereosinophilia; Kimura's disease; ventricular tachyarrhythmia 相似文献
Keywords: coronary vasospasm; hypereosinophilia; Kimura's disease; ventricular tachyarrhythmia 相似文献
63.
Objectives: The study aimed to (1) elucidate the energetic adaptations of Huli people by comparing the three sub-populations in relation to their diversified natural and socioeconomic environment, based on energy expenditure and time allocation data; and (2) assess the applicability of a new index of physical exertion levels of activities (physical exertion index: PEI). Research design and methods: Married males and females (n = 43) were selected, including 14 from a hilly village, 13 from a flat swampy village and 16 migrants to a town. Continuous heart rate (HR) monitoring, in conjunction with minute-by-minute observation of activities, was undertaken. Total energy expenditure was assessed by flex-HR method and physical activity level (PAL) was calculated as multiples of basal metabolic rate. Observed activities were divided into 15 categories and the PEI was calculated for each: PEI= (mean HR of a categorized activity)/(flex HR) x 100. Results : No significant difference was found in PAL among the three sub-populations: 1.77, 1.92 and 1.81 for men and 1.78, 1.98 and 1.66 for women in hilly and flat villages, and a town, respectively. The comparison of the two village groups revealed that hilly terrain did not affect substantially the physical intensity of agricultural activities. On the other hand, the town migrants were engaged in sedentary paid jobs and these were less energy consuming than agricultural work. However, the different energy costs between paid jobs and agricultural work were offset by longer work time in the town group than in the two village groups (251 vs 50 or 70 min in males; 258 vs 152 or 138 min in females), resulting in similar PAL in the three groups. Conclusions: Despite contrasting natural and social environments, no significant difference was found in daily physical activity level among the three sub-populations (two villages and a town) of Huli-speaking people in Papua New Guinea Highlands. 相似文献
64.
Ken Higashimoto Toshiyuki Maeda Junichiro Okada Yasufumi Ohtsuka Kensaku Sasaki Akiko Hirose Makoto Nomiyama Toshimitsu Takayanagi Ryuji Fukuzawa Hitomi Yatsuki Kayoko Koide Kenichi Nishioka Keiichiro Joh Yoriko Watanabe Koh-ichiro Yoshiura Hidenobu Soejima 《European journal of human genetics : EJHG》2013,21(11):1316-1319
Perlman syndrome is a rare, autosomal recessive overgrowth disorder. Recently, the deletion of exon 9 and other mutations of the DIS3L2 gene have been reported in patients; however, the mechanism behind this deletion is still unknown. We report the homozygous deletion of exon 9 of DIS3L2 in a Japanese patient with Perlman syndrome. We identified the deletion junction, and implicate a non-allelic homologous recombination (NAHR) between two LINE-1 (L1) elements as the causative mechanism. Furthermore, the deletion junctions were different between the paternal and maternal mutant alleles, suggesting the occurrence of two independent NAHR events in the ancestors of each parent. The data suggest that the region around exon 9 might be a hot spot of L1-mediated NAHR. 相似文献
65.
66.
67.
Radiofrequency ablation of pulmonary tumors and normal lung tissue in swine and rabbits 总被引:5,自引:0,他引:5
OBJECTIVE: The purpose of the present study was to examine the following during radiofrequency ablation (RFA): (1) the risk of hemorrhage from intrapulmonary large vessels; (2) the risk of incomplete ablation of pulmonary tumors; and (3) the late effect on lung tissue. MATERIALS AND METHODS: A 17-gauge, cool-tip-type radiofrequency electrode was used. The damage to the vessels and bronchi was examined by the injection of a colored silicone rubber, a liquid compound that hardens after injection. To examine the risk of hemorrhage from intrapulmonary large vessels, RFA was conducted at eight sites near the central pulmonary vessels in two swine. To examine the risk of an incomplete ablation for pulmonary tumors, 10 pulmonary nodules were made from a gelatin mixture in another two swine and were treated by RFA. To examine the late effect on lung tissue, RFA was conducted on the peripheral lung in 10 rabbits, and then the ablated regions were examined on days 1, 7, 14, 21, and 28 after RFA. RESULTS: The use of colored silicone rubber enabled us to examine the intrapulmonary vessels and bronchi for opening and leakage. RFA did not damage the large intrapulmonary vessels, even when they were located within the ablated regions. Lung tissue surrounding the gelatin nodules was hardly ablated over its entire circumference. Six of 10 gelatin nodules (60%) showed nonablated areas on the peripheral edges of the nodules. From 21 days after RFA, the ablated rabbit lung formed noninfectious cavities by communicating with the surrounding bronchi. CONCLUSION: It was improbable for hemorrhage to occur even when RFA was conducted near the large intrapulmonary large vessels. Because an incomplete ablation that left tumor cells at the site of ablation could occur during surgery due to the difficulty of ablating the entire tumor circumference, CT scan-guided RFA would be preferable to a surgical approach for making a safe margin. Cavity formation can occur beginning 21 days after RFA, which should be carefully followed up in a clinical setting to identify infection, especially in immunocompromised patients. 相似文献
68.
Yoshinobu Kariya Midori Oyama Mikio Ohtsuka Nobuyuki Kikuchi Yasuhiro Hashimoto Toshiyuki Yamamoto 《Fukushima journal of medical science》2020,66(3):119
α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression. 相似文献
69.
Koji Shindo Shinichi Aishima Kenoki Ohuchida Minoru Fujino Yusuke Mizuuchi Masami Hattori Takao Ohtsuka Shoji Tokunaga Kazuhiro Mizumoto Masao Tanaka Yoshinao Oda 《Virchows Archiv : an international journal of pathology》2014,465(3):265-273
A thickened, enhanced cyst wall on imaging examinations is one of the “worrisome features” described in the consensus guidelines for management of intraductal papillary mucinous neoplasm of the pancreas (IPMN). Podoplanin (PDPN) expression by cancer-associated fibroblasts is known to be an indicator of poor prognosis in some types of cancer. We performed immunohistochemical staining for alpha-smooth muscle actin (α-SMA) in IPMN lesions and determined the pathological wall thickness by measuring the thinnest and thickest α-SMA-positive parts of the wall of the largest cyst in each case, and the mean of these two values was recorded as the wall thickness. The thickness of the pathological wall increased with progression from IPMN with low-grade dysplasia to IPMN with an invasive carcinoma. The pathological wall was thicker in IPMN with main duct involvement, nongastric-type IPMN, and IPMN with mural nodules. We also stained for PDPN and assessed the thickness of cyst wall staining as for α-SMA. The thickness of the PDPN-positive cyst wall varied in a pattern similar to the thickness of the α-SMA-positive pathological cyst wall. PDPN-positive stromal fibroblasts in the invasive component of IPMN-IC were evaluated as a ratio to α-SMA-positive fibroblasts. A high ratio (>50 %) of PDPN-positive stromal fibroblasts was a predictor of poor outcome. PDPN expression in the cyst wall correlates with the progression of IPMN. PDPN may be a significant prognostic marker of IPMN-IC. 相似文献
70.
K. Higashimoto K. Jozaki T. Kosho K. Matsubara T. Fuke D. Yamada H. Yatsuki T. Maeda Y. Ohtsuka K. Nishioka K. Joh H. Koseki T. Ogata H. Soejima 《Clinical genetics》2014,86(6):539-544
The IGF2/H19‐imprinting control region (ICR1) functions as an insulator to methylation‐sensitive binding of CTCF protein, and regulates imprinted expression of IGF2 and H19 in a parental origin‐specific manner. ICR1 methylation defects cause abnormal expression of imprinted genes, leading to Beckwith–Wiedemann syndrome (BWS) or Silver–Russell syndrome (SRS). Not only ICR1 microdeletions involving the CTCF‐binding site, but also point mutations and a small deletion of the OCT‐binding site have been shown to trigger methylation defects in BWS. Here, mutational analysis of ICR1 in 11 BWS and 12 SRS patients with ICR1 methylation defects revealed a novel de novo point mutation of the OCT‐binding site on the maternal allele in one BWS patient. In BWS, all reported mutations and the small deletion of the OCT‐binding site, including our case, have occurred within repeat A2. These findings indicate that the OCT‐binding site is important for maintaining an unmethylated status of maternal ICR1 in early embryogenesis. 相似文献