重组链激酶溶栓联合氯吡格雷治疗急性心肌梗死的临床观察

目的观察国产重组链激酶(r-SK)治疗急性心肌梗死时经加用口服氯吡格雷后的临床疗效以及其安全性。方法自2006年10月至2007年10月间的急性心肌梗死(AMI)患者36例经国产重组链激酶溶栓联合氯吡格雷治疗的血管再通率、急性期病死率、并发症以及不良反应发生率。结果梗死相关血管再通率为86%,梗死后心绞痛6%,严重心律失常8%,心源性休克3%,出血8%,30d病死率3%。无其他药物相关的严重不良反应及过敏反应发生。结论r-SK溶栓联合氯吡格雷治疗急性心肌梗死是安全有效的。     

PEG 6000处理对黄芩种子萌发和幼苗生长的影响

目的 以PEG 6000溶液模拟干旱胁迫条件,研究黄芩种子的萌发和幼苗生长对干旱胁迫的响应.方法 分别用10%和20%PEG 6000处理黄芩种子,对种子的含水量、萌发率、萌发势、萌发指数、幼苗鲜质量及各器官的长度进行测量.结果 PEG 6000处理对黄芩种子的含水量、萌发率和幼苗的生长均产生影响.结论 10%PEG6000浸种4 h、20%PEG 6000浸种1 h可以提高黄芩种子的萌发率,可以选择20%PEG 6000处理黄芩种子8 h作为模拟干旱条件,用于幼苗黄芩抗旱材料的选育工作.     

小儿推拿加针灸法治疗婴幼儿秋冬季腹泻

2006年10月～2007年11月收治秋冬季腹泻患儿20例，男12例，女8例，年龄4个月～2岁，病程≤2天。     

Pathological features of gastric cancer in Zhuanghe high-risk area in China during 1992–2005

Objective  To investigate the pathological features and chronological changes of 1003 cases with gastric cancer in Zhuanghe high-risk area during 1992–2005 and the relationship between the changes and etiology factors in order to make a clue for gastric cancer prevention. Methods  A total of 1003 gastric cancer specimens resected surgically between 1992–2005 in Zhuanghe Center Hospital were studied. The specimens were fixed in formalin and diagnosed by routine pathology. Results  The incidence of patients with gastric cancer was highest at age of 60–69, the next high was at age of 50–59 and it was significantly higher in male than in female (P<0.001), the ratio was 3.0:1. During the past 14 years, there were 159 (15.9%) EGC, 195 (19.4%) moderate and 649 (64.7%) advanced gastric cancer detected. In macroscopical features, type III remained dominant in EGC, the next was mixed type in EGC. In advanced gastric cancer the Borrmann’s type III remained the dominant, the next was type II. For nodal metastasis, positive cases were decreasing and negative were increasing in EGC, moreover negative cases were higher than positive ones each year. There was no obvious trend in advanced cancer but positive cases were higher than negative ones each year. In histological features, papillary, moderately and poorly differentiated tubular adenocarcinoma remained downtrend, mucus adenocarcinoma and undifferentiation cancer remained uptrend year after year. The radio of intestinal to diffuse type decreased from 0.78 to 0.62 during the past 14 years. Conclusion  There were significant chorological trends of pathological characteristic of gastric cancer in Zhuanghe high-risk area during the past 14 years. This work was supported by the National “Tenth-Five” Key Technologies R&D Program of China (2004BA703B04-02)     

HBV前C区部分序列亚克隆与FQ-PCR标准曲线的制备

目的探讨HBV前C区基因用于乙型病毒性肝炎早期诊断价值和意义并基于此靶基因制备荧光定量PCR标准曲线。方法设计特异性引物,将pBR322-HBV质粒前C区部分序列PCR产物AT亚克隆至pBS-T载体,提取和纯化质粒DNA,制作标准品并在FQ-PCR仪上得到标准曲线,进而对精确度和重复性进行统计分析。结果获得了预期希望的质粒。荧光定量PCR标准曲线的相关系数为0.995,线性范围5×103 copies/μl~5×109 copies/μl。批内、批间变异系数值均小于5%,精确度高,重复性好。结论HBV前C区基因用于乙型病毒性肝炎早期检测和诊断有一定价值和意义。且FQ-PCR标准曲线的成功制备,临床早期检测乙肝病毒感染奠定了基础。     

胃癌间质T细胞及其亚群和B细胞的免疫组化研究

本实验应用抗T_3、T_4和T_8细胞表面抗原及抗B细胞表面抗原的单克隆抗体、对38例胃癌术后冰冻切片和20例正常胃粘膜活检冰冻切片进行免疫组织化学染色,较为系统地研究了T_3、T_4和T_8细胞及B细胞的分布特点。T_3、T_4和T_8细胞与胃癌病理生物学行为、临床分期和预后的关系。     

丹参醇提物在大鼠体内的药动学研究

目的建立大鼠血浆中丹参酮ⅡA和隐丹参酮的HPLC-UV检测法,并研究丹参脂溶性成分在大鼠体内的药动学。方法以吉非罗齐为内标,血浆样品经简单的甲醇沉淀蛋白后,上清液直接进样测定。大鼠尾iv丹参醇提取物(以丹参酮ⅡA计为5 m g/只),测定各时点丹参酮ⅡA和隐丹参酮的血药浓度,绘制药-时曲线,采用3p87计算药动学参数。结果大鼠尾iv丹参醇提取物后,丹参酮ⅡA的主要药动学参数分别为:t1/2α为(0.088±0.024)h,t1/2β为(2.1±0.7)h;VC为(0.8±0.6)L;CL为(2.0±1.1)L/h-1;AUC0-4和AUC0-∞分别为(2.2±0.9)m g.h/L和(3.4±1.7)m g.h/L。结论丹参醇提取物iv给药后,丹参酮ⅡA的药-时曲线符合二室模型,血浆中丹参酮ⅡA迅速下降,表观分布容积大;隐丹参酮在血浆中消除快,药动学参数不易获得。     

胃癌及癌前疾病血清细胞纤维连接蛋白的检测及其临床意义

目的:探讨胃癌及癌前疾病患者血清细胞纤维连接蛋白(cFN)含量在不同胃疾病中变化情况及临床意义,及与幽门螺杆菌(HP)感染的相关性。方法:利用酶联免疫吸附试验检测1177例正常人、癌前疾病及胃癌患者血清cFN含量、胃疾病患者血清HP抗体含量。结果:血清cFN含量胃癌组明显高于正常人及胃良性疾病组(P<0.05),分化不良组显著高于分化良好组(P<0.01),淋巴结转移阴、阳性组比较差异无显著性(P>0.05)。胃疾病患者血清HP抗体检测,血清cFN含量,HP阴、阳性组比较差异无显著性,但在胃良性病变组中HP抗体阳性组较阴性组高,而胃癌组HP抗体阳性组较阴性组低。结论:血清cFN含量测定可用于胃良恶性疾病的鉴别,对胃癌诊断具有潜在的临床应用价值;胃癌患者血清cFN含量与胃癌分化程度密切相关,与胃淋巴结转移无关。血清cFN含量HP抗体阴性、阳性组比较差异无显著性,但胃良性疾病组,HP抗体阳性组较HP阴性组略高,胃癌组,HP抗体阳性组较HP阴性组略低,提示HP感染在胃良性疾病阶段和胃癌阶段,对血清cFN含量可能具有不同影响。由于血清HP抗体与其HP感染不一定平行,HP感染对血清cFN含量的影响有待于进一步深入探讨。     

化脓性脑膜炎患儿脑脊液NO与IL—8的相关性研究

目的：探讨一氧化氮（NO）与白细胞介素－8（IL－8）在化脓性脑膜炎（化脑）中的作用机制。方法：采用分光比色法和放射免疫分析法对38例化脑患儿及20例对照儿进行了脑液液中NO，IL－8的测定。结果：化脑患儿脑脊液NO，IL－8含量均显著高于对照组（P＜0．01），且NO与IL－8呈中度正相关关系（P＜0．01），结论：NO和IL－8协同参与了化脓性脑膜炎的感染过程。     

泰特在心内直视术中的心肌保护作用

目的观察心脏含血停搏液加入泰特（TAD）在心内直视术中的心肌保护作用。方法30例心内直视术患者随机分为两组,实验组在含血心脏停搏液中加入TAD（剂量600 mg）,对照组单用含血心脏停搏液,分别经主动脉根部或切开主动脉经冠状动脉窦直接灌注,观察诱导停搏时间、心脏复跳情况、术后机械通气时间及术后正性肌力药物的应用情况。结果实验组较对照组自动复跳率高,诱导停搏时间、术后多巴胺用量、机械辅助通气时间显著减少。结论TAD加入心脏停搏液中具有良好的心肌保护作用。