CD1d-restricted natural killer T cells are potent targets for human immunodeficiency virus infection

Invariant human natural killer T cells (NKT) express a restricted T-cell receptor (TCR) Valpha24Vbeta11 repertoire. These cells share both phenotypic and functional similarities between NK and T cells. Given the emerging role of NKT cells as critical cells in bridging the gap between innate and adaptive immunity, we examined their susceptibility to productive human immunodeficiency virus (HIV) infection by T-tropic, M-tropic, and primary isolates of HIV. We generated three human NKT cell clones (CA5, CA29, and CA31). Phenotypic characterization of these Valpha24+ Vbeta11+ clones indicated that they were predominately positive for CD4, CD161, HLA-DR, CD38, CD45RO, and CD95 expression. The NKT cell clones expressed significantly more surface CCR5 molecules/cell and lower CXCR4 molecules/cell than phytohaemagglutinin-stimulated peripheral blood mononuclear cells (PBMC). Consistent with the surface expression of CCR5 and CXCR4, the NKT clones were also selectively susceptible to HIV M-tropic, T-tropic, and primary isolate infection, as evaluated by both HIV p24 enzyme-linked immunosorbent assay and intracellular staining of HIV proteins. The amount of p24 production was dependent on the NKT clone studied and the HIV strain used. Clones CA29 and CA31 were also susceptible to HIV IIIB infection. The virions produced by these clones were able to productively infect PHA-stimulated PBMCs with the same kinetics as for primary infection of CD4+ blast. Collectively, this data demonstrates that NKT cells can be a target for productive HIV infection but with a lag in the time to peak p24 production.     

旅游者心理健康状况的调查分析

目的　评定旅游者的心理健康状况。方法　对我市某旅行社外出旅游者进行症状自评量表 ( SCL-90 )测试 ,并将结果与中国正常人常模进行比较。结果　旅游者在人际关系和焦虑两项因子分高于常模 ,其中人际关系敏感因子分差异显著( P<0 .0 5 ) ,精神病性因子分低于常模 ,并具有显著差异 ( P<0 .0 5 ) ;单独外出旅游者与结伴外出旅游者相比在人际关系敏感和焦虑两个因子方面具有显著性的差异 ( P<0 .0 5 )。结论　旅行社对旅游者的心理健康状况应引起重视 ,并对存在的心理问题进行必要的干预     

hTRT催化功能区基因克隆及其在肿瘤中的表达

目的 研究端粒酶蛋白ｈＴＲＴ基因在肿瘤中的表达及其意义。方法 用ＲＴ－ＰＣＲ法检查Ｈｅｌａ细胞与ＰＧ细胞的ｈＴＲＴ表达水平,将Ｈｅｌａ细胞的ｈＴＲＴ催化功能克隆,并进行测序比较,应用原位杂交技术对肿瘤组织中的ｈＴＲＴ和ｈＴＲ（端粒酶ＲＮＡ组分）基因进行检测。结果 Ｈｅｌａ细胞与ＰＧ细胞均有ｈＴＲＴ表达,Ｈｅｌａ细胞ｈＴＲＴ催化功能区ｃＤＮＡ序列与文献报道一致,原位杂交结果显示ｈＴＲＴ与ｈＴＲ的协同     

痘苗病毒VGF细胞膜外结构域在真核及原核细胞中的表达

目的为了进一步探索ＶＧＦ的生物学功能方法分别构建了痘苗病毒天坛株生长因子（Ｖａｃｃｉｎｉａｇｒｏｗｔｈｆａｃｔｏｒ，ＶＧＦ）基因细胞膜外结构域（ＶＧＦｓｌ）的真核及原核表达系统，在相应的细胞中获得了单独表达（真核）和融合表达（原核）。结果初步纯化的ＶＧＦｓｌ表达产物能够与上皮生长因子（ＥＧＦ）的受体结合，并刺激受体中酪氨酸残基发生磷酸化反应。与ＥＧＦ相比，ＶＧＦｓｌ表达产物刺激ＥＧＦ受体自身磷酸化的能力更强。结论上述结果为ＶＧＦ在病毒感染过程中的作用以及ＶＧＦ作为新型多肽类药物的研究提供了有价值的线索。     

微创DHS内固定与传统DHS内固定治疗老年股骨粗隆间骨折比较研究

目的:对微创动力髋螺钉(dynamic hip screw,DHS)内固定和传统DHS内固定治疗老年股骨粗隆间骨折的效果进行比较.方法:将91例老年股骨粗隆间骨折患者随机分为两组,传统组46例采用传统方式置入内固定物治疗,微创组45例采用微创方式置入内固定物.对两组手术创伤大小、术后并发症、骨折愈合及功能恢复情况进行比较.主要通过考察手术切口长度、出血量、输血量、血红蛋白下降幅度、术后术区肢体肿胀情况、血沉变化等来反映手术创伤大小.结果:两组手术创伤差异有统计学意义,传统组大于微创组(P＜0.05).微创组有2例复位不良,传统组有3例复位不良,两组均无深部血肿发生.传统组围手术期死亡2例,其余患者均顺利出院,获12～30个月随访,平均17.1个月.两组各有1例在随访期间死于其它内科疾病,其余骨折均获愈合.术后平均骨折愈合时间微创组3.6个月,传统组3.8个月.术后1年髋关节功能优良率微创组84.1%,传统组79.1%,两组差异无统计学意义(P＞0.05).结论:微创DHS内固定治疗老年股骨隆间骨折,手术创伤小,有利于围手术期恢复和髋关节功能康复.     

Trabecular Shear Stresses Predict <Emphasis Type="Italic">In Vivo</Emphasis> Linear Microcrack Density but not Diffuse Damage in Human Vertebral Cancellous Bone

Linear microcracks and diffuse damage (staining over a broad region) are two types of microscopic damage known to occur in vivo in human vertebral trabecular bone. These damage types might be associated with vertebral failure. Using microcomputed tomography and finite element analysis for specimens of cancellous bone, we estimated the stresses in the trabeculae of human vertebral tissue for inferosuperior loading. Microdamage was quantified histologically. The density of in vivo linear microcracks was, but the diffuse damage area was not, related to the estimates of von Mises stress distribution in the tissue. In vivo linear microcrack density increased with increasing coefficient of variation of the trabecular von Mises stress and with increasing average trabecular von Mises stress generated per superoinferior apparent axial stress. Nonlinear increase in linear crack density, similar to the increase of the coefficient of variation of trabecular shear stresses, with decreasing bone stiffness and bone volume fraction suggests that damage may accumulate rather rapidly in diseases associated with low bone density due to the dramatic increase of shear stresses in the tissue. © 2003 Biomedical Engineering Society. PAC2003: 8719Rr, 8719Xx, 8759Ls, 8759Fm, 8710+e     

氯霉素乙酰基转移酶双抗体夹心ELISA检测方法的建立

目的 建立氯霉素乙酰基转移酶（chloramphenicol acetyltransferase,CAT)双抗体夹心ELISA的实验室检测方法。方法 从质粒pBLCAT6经PCR扩增CAT基因序列，插入到原核表达质粒pGEX-2T中，在大肠埃希菌DH5α中诱导表达；以纯化的融合蛋白GST-CAT为抗原免疫实验用兔，得到的CAT抗血清进行生物素标记，并在此基础上利用生物素链和亲和素放大系统建立双抗体夹心ELISA法，构建不同YY1结合位点突变的HPV16LCRCAT报道质粒，体外瞬时转染真核细胞，提取胞质蛋白，以建立的方法进行CAT表达检测。结果 SDS-PAGE显示表达的GST-CAT融合蛋白相对分子质量约为54000；制备的CAT抗血清可有效地识别原核及哺乳动物细胞表达的CAT蛋白；在不同启动子及调节序列的控制下，利用建立的CAT检测技术证明在瞬时转染的细胞中可诱导2-8倍的CAT表达增强。结论 建立的双抗体夹心ELISA方法可敏感地反映上游序列的启动子活性，以及启动子调节序列变化对启动子活性的影响，使CAT作为报道基因的研究更为简单化。     

A Bcl-2-dependent molecular timer regulates the lifespan and immunogenicity of dendritic cells

The lifespan of antigen-bearing dendritic cells (DCs) is determined by signals from pathogens and T cells. These signals regulate DC survival by modulating expression of Bcl-2 family proteins. Toll-like receptors and T cell costimulatory molecules both trigger a DC survival pathway that is dependent on Bcl-x(L). However, Toll-like receptors uniquely increase expression of Bim and trigger cell death by a pathway that is blocked by Bcl-2. This pathway serves as a molecular 'timer' that sets the lifespan of DCs and regulates the magnitude of T cell responses in vivo. Thus, signals derived from the innate and acquired immune systems control DC lifespan and immunogenicity by distinct molecular mechanisms.     

氨基葡萄糖胶囊干预膝骨关节炎患者外周血单个核细胞中软骨代谢相关基因的表达

背景:目前关于氨基葡萄糖的研究多集中于对膝骨关节炎的治疗作用,但关于其对膝骨关节炎患者外周血中软骨代谢相关基因影响的研究有限。目的:观察氨基葡萄糖胶囊对膝骨关节炎的治疗效果及对软骨代谢相关基因表达的影响。方法:选取2017年3月至2019年2月郑州大学附属郑州中心医院收治的90例膝骨关节炎患者,另选取同期医院体检中心收入的40例健康受试者,检测并对比健康受试者与膝骨关节炎患者治疗前外周血单个核细胞中软骨寡聚基质蛋白、Ⅱ型胶原纤维α1、聚糖蛋白、特异性组织抑制物3基因表达水平。采用随机数表法将膝骨关节炎患者分为常规组和研究组,分别给予双氯芬酸钠缓释片、双氯芬酸钠缓释片+氨基葡萄糖胶囊治疗12周,对比治疗前后2组外周血单个核细胞中各基因表达水平、Lequesne指数,统计治疗期间2组不良反应发生情况。结果与结论:①与健康受试者比较,膝骨关节炎患者外周血单个核细胞中软骨寡聚基质蛋白、特异性组织抑制物3 mRNA相对表达量升高(P<0.05),Ⅱ型胶原纤维α1、聚糖蛋白mRNA相对表达量降低(P<0.05);②治疗前研究组和常规组外周血单个核细胞中各基因表达水平比较,差异均无显著性意义(P>0.05);治疗后2组外周血单个核细胞中软骨寡聚基质蛋白、特异性组织抑制物3 mRNA相对表达量均低于治疗前,且研究组低于常规组(P<0.05);治疗后2组外周血单个核细胞中Ⅱ型胶原纤维α1、聚糖蛋白mRNA相对表达量均高于治疗前,且研究组高于常规组(P<0.05);③治疗前研究组和常规组Lequesne指数比较,差异均无显著性意义(P>0.05);治疗后2组Lequesne指数均低于治疗前,且研究组低于常规组(P<0.05);④研究组和常规组不良反应发生率比较,差异均无显著性意义(P>0.05);⑤结果表明,氨基葡萄糖胶囊可有效改善膝骨关节炎患者临床症状且安全可靠,可能通过抑制软骨寡聚基质蛋白、特异性组织抑制物3基因表达,促进Ⅱ型胶原纤维α1、聚糖蛋白基因表达实现的。     

多发性骨髓瘤患者血清IL-6活性与急性相蛋白浓度的相关性研究

运用敏感的B_9细胞增殖试验检测了81例多发性骨髓瘤(MM)患者血清IL-6活性,同时分析了标本的几种急性相蛋白含量,结果表明,68％MM患者血清中IL-6活性大于5μ/ml(正常对照为5μ/ml以下),几种急性相蛋白中C-反应性蛋白(CRP)在MM时升高(P<0.01),平均达正常对照组的17倍以上,MM患者补体C_4与正常对照组无差异(p>0.05),C_3、白蛋白及转铁蛋白在MM时分别比正常下降24.42％、38.83％和32.80％,且与疾病分期有关,在血清IL-6大于5μ/ml的55例中,IL-6活性与CRP、C_3、白蛋白的相关系数分别为0.46,-0.34和-0.29,IL-6与转铁蛋白浓度相关不明显。本文结果提示:CRP、C_3及白蛋白等含量的变化可作为反映MM病情的简易而敏感的指标。