急性白血病患者辨证分型与血象、骨髓象及血、尿环核苷酸关系的临床观察

把59例急性白血病患者分为温毒瘀血、痰热症瘕、气阴两虚三型,观察其盘象、骨髓象和血、尿环核苷酸变化,发现各型与正常人比较均有显著差异,但程度并不一致。对32例患者治疗前后观察,气阴两虚型的缓解率较高,而温毒瘀血型较差。实验认为急白中医分型具有分子病理学基础。     

Recombinant adeno-associated virus serotype 2 effectively transduces primary rat brain astrocytes and microglia

Recombinant adeno-associated virus-2 (rAAV2) under control of the chicken beta actin promoter/truncated CMV enhancer (CBA) was investigated for its ability to transduce primary cultures of rat brain neurons, microglia and astrocytes. This vector was highly effective in all three cell types in heparin-sensitive manners (astrocytes, microglia and neurons transduced by >98%, 75%, and 95%, respectively). However, astrocytes co-cultured with neurons were not transduced. rAAV2/CBA is an important new method for genetic manipulation of brain cells, though this may be modulated by interactions among cell types.     

人子宫内膜癌裸鼠模型的建立及其生物学特性的研究

目的 为子宫内膜癌的体内实验及临床研究提供动物实验模型。方法 将人子宫内膜癌手术切除标本移植于BALB／C（nu/nu)裸鼠皮下,传代移植成功后进行鼠间传代。结果 共进行了14例手术标本移植,原代移植成功率为42．9％,自第5代以后,移植瘤的传代成功率均达到100．0％。至2001年5月,共有5株移植瘤传至48－63代,连续传代的移植瘤仍保持原癌组织病理形态特点及人类肿瘤染色体的特点。在传代过程中,裸鼠模型SL－1、SL－2、SL－3的DNA倍体和DNA指数均无明显变化,而SL-4、SL－5随着传代次数的增加,DNA倍体和DNA指数均发生明显变化。5个移植瘤模型雌激素受体（ER）、孕激素受体（PR）表达均为阴性;p53蛋白表达：SL－3、SL－5阴性,SL－1弱阳性,SL－2、SL－4强阳性;P16表达：SL－1、SL－4、SL－5阴性,SL-3阳性,SL－2强阳性;c-erbB－2表达：SL-3、SL-5阴性,SL－2弱阳性,SL－1、SL－4强阳性。结论 人子宫内膜癌手术标本经48－63代传代,成功建立了5株人子宫内膜癌裸鼠皮下移植瘤模型,为开展人类子宫内膜癌的体内实验及临床研究,提供了理想的动物模型。     

不同脱敏方法对牙体预备后活髓牙的影响

目的 在口腔修复临床中,固定义齿修复的牙体预备时,为防止患者牙本质过敏反应,甚至引起牙髓炎,通过采用不同的脱敏方法进行对比,寻找既简便,又不需特殊设备,且效果良好的脱敏方法,以保护牙体预备后的活髓,缓冲因修复给患者造成的痛苦.方法 将78例患者132颗活髓基牙随机分为A、B、C三组,经牙体预备后,对每颗基牙常规隔湿、消毒,干燥牙面.三组分别用75%氟化钠甘油涂擦加Nd:YAG激光照射、极固宁TM和Cluma脱敏剂对活髓基牙进行脱敏.最后用氧化锌水门汀粘固暂时冠,1周后,修复体完成,再用聚羧酸锌水门汀永久粘固.结果 1周后有效率:A组97.37%;B组95.65%;C组100%.3个月后有效率:A组81.58%;B组82.61%;C组93.75%.C组与A、B两组相比,1周后无显著性差异(P＞0.05);3个月后有显著性差异(P＜0.05).结论 Nd:YAG激光与氟化钠甘油联合应用脱敏和极固宁TM脱敏没有Gluma脱敏剂疗效好.且Nd:YAG激光联合氟化钠甘油使用时需要激光治疗机辅助;极固宁TM脱敏剂需两种液体交替使用,操作繁琐.而Gluma脱敏剂材料组份单一,使用方便,作用迅速,见效快,疗效持久,效果最好. 结果 1周后有效率:A组97.37%;B组95.65%;C组100%.3个月后有效率:A组81.58%;B组82.61%;C组93.75%.C组与A、B两组相比,1周后无显著性差异(P＞0.05);3个月后有显著性差异(P＜0.05).结论 Nd:YAG激光与氟化钠甘油联合应用脱敏和极固宁TM脱敏没有Gluma脱敏剂疗效好.且Nd:YAG激光联合氟化钠甘油使用时需要激光治疗机辅助;极固宁TM脱敏剂需两种液体交替使用,操作繁琐.而Gluma脱敏剂材料组份单一,使用方便,作用迅速,见效快,疗效持久,效果最好. 结果 1周后有效率:A组97.37%;B组95.65%;C组100%.3个月后有效率:A组81.58%;B组82.61%;C组93.75%.C组与A、B两组相比,1周后无显著性差异(P＞0.05);     

新生儿缺氧缺血性脑病43例临床与预后分析

分析新生儿缺氧缺血性脑病（ＨＩＥ）４３例。有宫内窘迫史者占５８１％,出生重度窒息占８１４％。ＨＩＥ轻度１０例,中度２６例,重度７例。合并心肌损害达４１７％。治愈好转率为８１３％。病死率７０％。随访２４例,轻度ＨＩＥ预后好,中度有明显后遗症者为６７％,重度预后不良。认为加强围生期保健,提高产科质量,进行新法复苏及复苏后处理是降低ＨＩＥ发病率的关键。诊治中应重视心肌损害。使用胞二磷胆碱等脑细胞代谢激活剂辅治ＨＩＥ效果肯定。对于预后,强调早期治疗,早期评分、早期随访、早期干预是改善重度ＨＩＥ预后的几个重要环节。     

聚合酶链反应和原位杂交检测成人肾炎肾组织HBV DNA

目的 探讨乙型肝炎病毒（ＨＢＶ）感染与成人肾小球肾炎（肾炎）之间的联系,及研究ＨＢＶ对肾脏的致病作用。方法 应用聚合酶链反应（ＰＣＲ）和原位杂交（ＩＳＨ）技术检测３９例血清ＨＢＶ标志物阳性的成人肾有活检组织ＨＢＶ ＤＮＡ的存在状态。结果 用ＰＣＲ法测定肾活检组织抽提物ＨＢＶ ＤＮＡ１７／３９例阳性（４３．６％）,其中５例ＰＣＲ阳性者再且地高辛素标记的ＨＢＶ ＤＮＡ探针原位杂交,测定肾活检石腊包埋切     

运钞车押运员MMPI测查结果比较分析

目的分析评价运钞车押运员的心理健康状态,为提高他们的心理素质提供理论依据。方法对476名押运员进行明尼苏达多项个性问卷（MMPI）测查,测查结果与中国常模比较,并进行组间比较。结果各个量表均出现不同程度的高分,具有显著性差异,司机和解款员与持枪押运员两组之间比较没有差异性。结论运钞车押运员存在心理问题,应予关注,并建立心理援助系统。     

腺苷处理人类T淋巴细胞亚群表面标纪的单克隆抗体的检测

腺苷(adenosine)是机体本身所存在的一种生物活性物质,有着较广泛的生物学效应。在免疫系统中,腺苷能够抑制机体中的许多免疫反应。近年来,许多实验发现,经腺苷处理了的人类外周血T淋巴细胞,有部份T细胞会丧失与绵羊红细胞(SRBC)形成玫瑰花的能力,而另一部份T细胞(主要部份)仍保持着与SRBC形成玫瑰花的能力。前者被     

Dissecting the roles of DR4, DR5 and c-FLIP in the regulation of Geranylgeranyltransferase I inhibition-mediated augmentation of TRAIL-induced apoptosis

Background  

Geranylgeranyltransferase I (GGTase I) has emerged as a cancer therapeutic target. Accordingly, small molecules that inhibit GGTase I have been developed and exhibit encouraging anticancer activity in preclinical studies. However, their underlying anticancer mechanisms remain unclear. Here we have demonstrated a novel mechanism by which GGTase I inhibition modulates apoptosis.     

Zinc influences on brain development, pituitary and thyroid function in iodine-deficient pregnant and neonatal rats*

BACKGROUND： Zinc （Zn） has been shown to greatly influence brain development. Zn supplements may reduce injury to cell membranes of the thyroid gland due to iodine deficiency. OBJECTIVE： To establish an iodine deficiency rat model using low-iodine food, which was supplemented with compound Zn and Zn gluconate, to observe the effects of Zn on brain development, as well as pituitary gland and thyroid gland function in iodine-deficient rats. DESIGN, TIME AND SETTING： Randomized grouping study of neural development was performed in the central laboratory of Shandong Institute for Prevention and Treatment of Endemic Disease from 1998 to 1999. MATERIALS： A total of 270 Wistar, female rats, one month after weaning, were used in this study, including 150 pregnant and 120 neonatal rats. Rats were randomly divided into six groups： normal control, model, iodine, compound Zn, iodine and compound Zn, and zinc gluconate. Each group contained 25 pregnant rats and 20 neonatal rats. METHODS： The pregnant rats and 20 neonatal rats, and well as the normal group, were fed standard chow and allowed free access to tap water （containing 5 u g/L iodine and 1 mg/L Zn）. The remaining five groups were fed low-iodine chow. However, the model group received distilled water, the iodine group received potassium-iodide distilled water （containing 300 u g/L iodine）, the compound Zn group received distilled water and intragastrically administrated 10 mL/kg compound Zn solution, once per day, the iodine and compound Zn group received distilled water with 300 u g/L iodine and intragastrically administrated 10 mL/kg compound Zn solution, once per day. All treatments lasted 90 days. MAIN OUTCOME MEASURES： All pregnant rats were sacrificed on the day 21 of pregnancy. Body mass number and rate of fetal absorption, as well as fetal death and malformation, were determined. Thyroid and pituitary gland weights were measured, as well as serum levels of thyroid hormone, gonadotropin, and sex hormones. In the experimental study of ne