PC12细胞化学缺氧复氧损伤与缺氧诱导因子1表达的关系

目的 研究缺氧诱导因子1(HIF1)在PC12细胞化学缺氧复氧损伤中的作用。方法 在培养液中加入和去除氯化钴模拟化学缺氧和复氧，以乳酸脱氢酶(LDH)漏出和细胞超微结构改变作为细胞损伤指标，观察化学缺氧和复氧后不同时间细胞损伤和HIF1 α蛋白变化。结果 在氯化钴模拟化学缺氧实验中，LDH漏出明显增加，8h达高峰，随后逐渐下降，电镜结果与LDH改变相一致，HIF1 α蛋白表达在化学缺氧后2，4，8和12h均明显增加，8h达高峰，提示化学缺氧8h后细胞损伤逐渐减轻可能与HIF1 α蛋白水平升高有关。在模拟复氧实验中，LDH和细胞形态学改变都显示化学复氧8h细胞损伤最为严重，而HIF1 α蛋白表达在化学复氧4和8h均明显下降，提示细胞化学复氧损伤可能与HIF1 α蛋白水平下降有关。结论 HIF1对神经细胞化学缺氧复氧损伤具有保护作用。     

体外培养诱导外周血淋巴细胞促进大鼠面神经再生的实验研究

目的:通过在神经缺损处局部回输体外分离培养纯化的淋巴细胞,了解此种方法促进面神经损伤修复的效果。方法:将20只Wistar大鼠的面神经颊支剪断并立即缝合(其余3支反折缝合)制成面神经损伤模型大鼠,将其分成淋巴细胞组和对照组,每组10只,每组再分成2周组和8周组。淋巴细胞组局部回输体外分离培养的外周血淋巴细胞,对照组作对照。于2周和8周测定面神经颊支-触须肌复合动作电位传导速度,辣根过氧化物酶(HRP)神经逆行示踪测定面神经核团的神经元阳性数目。结果:淋巴细胞组面神经颊支-触须肌复合动作电位传导速度8周时为0.64±0.07,与对照组(0.56±0.07)相比,差异有统计学意义(P<0.05)。HRP神经逆行示踪测定面神经核团神经元阳性数目,淋巴细胞组2周及8周与对照组同时间段相比差异均无统计学意义(均P>0.05)。结论:体外分离培养纯化的淋巴细胞在局部应用于神经损伤处对面神经再生修复可起一定的促进作用。     

实验性腹主动脉瘤瘤体外径与iNOS相关性分析及分期研究

Objective To construct new model of experimental abdominal aortic aneurysm (AAA) and detect the AAA outer diameter and the change of induce nitric oxide synthase(iNOS) in the abdominal aor-tic aneurysm in different period,and analysis the correlation between experimental AAA and iNOS.Methods During the operation of the experiment group,the vascular prosthesis of PTFE was implanted to the rabbit abdominal aorta to form an aneurysm,while sham operation was done in the control group.The tissue of ab-dominal aortic aneurysm was harvested in 1 d,7d,14d,and 28d after operation,respectively.The tissue bo-mogenate concentration of iNOS in the abdominal aortic aneurysm were detected by enzyme linked immu-nosorbent assay (ELISA).Results In experimental group,mean concentration of the tissue bomogenate concentration of iNOS in the abdominal aortic aneurysm in 1 d,7 d,14 d,28 d were (22.129 ±2.518)μ/mL,(27.337±5.321) μ/mL,(36.047±4.584)μ/mL,(44.756±1.799)μ/mL,respectively;In control group,that was (12.499±1.807)μ/mL.The concentration of iNOS in experimental group was significantly higher than that of control group (P < 0.01).The difference during the experimental group all had statisti-cal significance (P < 0.05).Conclusion It is possible that iNOS has some biological function during the formation and progression of the abdominal aortic aneurysm.     

大隐静脉曲张激光闭合术时高位结扎与未高位结扎的近期复发率对比研究

目的 研究大隐静脉激光闭合术时高位结扎与未高位结扎对近期复发率的影响,明确大隐静脉激光闭合术时是否有必要附加大隐静脉高位结扎术。方法 将2004年3月至2006年3月接受大隐静脉激光闭合术的215例患者随机分为两组,A组：大隐静脉高位结扎加激光闭合术,B组：单纯大隐静脉激光闭合术,比较其术后复发率及并发症的区别。结果 随访6～29个月,1例失访,无深静脉血栓及肺栓塞发生。A组复发4例,复发率3.8%,B组复发9例,复发率8.3%,两组复发病例病因均为原发性深静脉瓣膜功能不全,单纯大隐静脉曲张术后无复发。经统计学分析,两组复发率无统计学意义（P〉0.05）。结论 没有证据表明行大隐静脉激光闭合术时首先行高位结扎更有优势,未高位结扎并不增加深静脉血栓形成及肺栓塞的发生机会。     

Smoothing the mosaic subclinical melanoderma by calcipotriol

BACKGROUND: The observation of photo-exposed skin under ultraviolet light reveals a mosaic pattern of varying intensity in epidermal melanization. Several patterns of mosaic subclinical melanoderma (MSM) have been described using a specially designed CCD camera and the ultraviolet light-enhanced visualization (ULEV) method. Vitamin D(3) and its analogues influence the biology of keratinocytes and melanocytes. OBJECTIVE: To assess the effect of calcipotriol on MSM. METHODS: This randomized split-face study was conducted in 27 men to compare the effect of once daily applications of 5% calcipotriol cream or a moisturizing cream on the heterogeneity of facial MSM. Computerized image analysis of video images was used at 1-month intervals before and during a 2-month treatment, as well as during a 3-month follow-up. RESULTS: At both sites, the average melanin content of the epidermis showed no significant change over time. However, the mottled appearance was smoothened at the calcipotriol site, whereas it was increased at the site receiving the moisturizer. CONCLUSION: The decreased heterogeneity in MSM after calcipotriol applications suggests a control of the epidermal melanocyte unit by the vitamin D(3) derivative.     

认知疗法合并黛力新治疗脑卒中后焦虑的疗效

目的评价认知疗法合并黛力新(氟哌噻吨/美利曲辛)治疗脑卒中后焦虑的疗效。方法将80例脑卒中后焦虑患者随机分为治疗组(n=40)和对照组(n=40),分别给予认知疗法合并黛力新、单用黛力新治疗8周。用汉密顿焦虑量表、副反应量表分别评定疗效和副反应。结果治疗4、8周时,治疗组汉密顿焦虑量表评分(16.08±7.45,8.80±4.73)明显低于对照组(19.80±8.26,11.35±5.03),P<0.05;临床总疗效无论治疗4、8周,治疗组均优于对照组,P<0.01。结论认知疗法合并黛力新治疗脑卒中后焦虑的疗效较好。     

红景天对高原去势大鼠骨质疏松症的血清IL-6、TNF-α和尿DPD影响

目的:探讨高原高寒地区低氧环境去势大鼠骨质疏松症与血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和尿脱氧吡啶酚(DPD)的关系及红景天预防骨质疏松的作用。方法:选用Wistar雌性大白鼠75只,分别摘除卵巢或假性去卵巢。分为A组(假手术组),B组(切除卵巢组),C组(切除卵巢 尼尔雌醇组),D组(切除卵巢 红景天混合饲料组),E组(切除卵巢 红景天浸液组)。每组15只,术后在海拔3100m青海海北州地区饲养,术后3个月处死,应用放免方法测定血清IL-6、TNF-α和化学发光法测定尿中DPD含量。结果:B组血清IL-6、TNF-α和尿DPD含量显著高于A、C、D、E组。C、D、E组血清IL-6、TNF-α和尿DPD含量与A组比较差异无显著性统计学意义。结论:红景天的抗高原骨质疏松症的作用可能与降低血清IL-6、TNF-α含量和尿中DPD浓度有关。     

Comparative study of renal sodium transport between ouabain-hypertensive rats and ouabain-nonhypertensive rats

Objective: To compare renal sodium transport, using fractional excretions of lithium(FELi) as a marker of proximal tubule sodium reabsorption, between hypertensive and non-hypertensive ouabain-treated rats and further to elucidate the role of ouabain in pathogenesis of hypertension. Methods: Thirty male Sprague-Dawley rats weighting 180-200 g were randomly divided into normal control group and ouabain treated group. Rats were infused with 1 ml/kg·d normal saline or 27. 8μg/kg·d ouabain in-traperitoneally once a day respectively. Systolic blood pressure (SBP), heart rate and body weight were recorded weekly. Rats were sacrificed 6 weeks after treatment. Blood and 24-hour urine sample were collected to measure the serum and urinary concentration of sodium, trace lithium and creatinine. Endogenous creatinine clearance rate(Ccr), fractional excretions of sodium (FENa), fractional excretions of lithium (FELi) and fractional reabsorption of sodium in the postproximal tubules (FDRNa) were calculated. Ouabain levels of plasma and renal tissue, plasma renin activity, angiotensin I and aldosterone concentration were determined. Results: 65% of the ouabain-treated rats achieved significantly higher SBP after 4 weeks, compared with that of the saline control groups or self baseline (P<0. 01). But in the other 35% of the ouabain-treated rats, their SBP was similar with control group during the experiment (P>0. 05). The body weight, heart rate and food intake between the 3 groups were no significant differences (P> 0. 05). FELi and FDRNa were significantly lower in ouabain-hypertensive group compared with ouabain-non-hypertensive group and control group(P<0. 01 and P<0. 05). The FEu and FDRn, of ouabain-nonhyper-tensive groups were similar with control group(P>0. 05). Ccr and FENa were comparable between the 3 groups (P>0. 05). Plasma and renal tissue ouabain levels, plasma renin activity, angiotensin I and aldosterone contents in ouabain-hypertensive rats were comparable with ouabain-nonhypertensive rats. Conclusion: Increase of proximal tubule sodium reabsorption play an important role in the pathogenesis of ouabain-hypertensive rats. The change of renal sodium transport may result from regulation to renal Na+ , K + -ATPase by ouabain.     

中国维和部队士兵性观念和性心理的调查研究

目的:掌握中国维和部队士兵性观念和性心理状况,力图加以正确引导和心理干预。方法:在维和任务中期,采用自编问卷,对314名中国驻利比里亚维和士兵进行性观念和性心理方面的调查。结果:超过90%的士兵性知识来源于自学,部分士兵性知识相对缺乏,通过集训的学习,仍有77%的战士对“艾滋病”有恐惧感。与未婚士兵相比,已婚士兵显示出更成熟的性心理(P<0.05);而性观念上差异没有显著性(P>0.05)。结论:针对不同群体进行有效的心理干预和心理疏导,能让士兵理智地对待自己的性欲和愿望,把性能量升华为内在的工作动力,创造性地开展维和工作。     

心率及重建相位窗对多层螺旋CT冠状动脉造影图像质量的影响

目的 探讨心率及重建相位窗对多层螺旋CT冠状动脉造影图像质量的影响。方法 80例健康体检者行16层螺旋CT回顾性心电门控条件下冠状动脉造影。结果 每位患者的4条冠状动脉分支(左冠状动脉主干、左前降支、左回旋支、右冠状动脉)分别用于图像质量分析。心率≤60次／min者，有82．1％(46／56支)的图像可用于诊断；心率61～70次／min者，有63．4％(104／164支)的图像可用于诊断；心率71～80次/min者，有41．20h，(28／68支)的图像可用于诊断；心率80次／min以上者有31．2％(10／32支)的图像可用于诊断。左冠状动脉前降支在60％～70％的重建相位窗时图像质量最佳，左冠状动脉回旋支在50％～60％时最佳，右冠状动脉重建相位窗为50％～70％较满意。结论心率及重建相位窗对多层螺旋CT冠状动脉造影图像质量有重要影响作用。