恒河猴坐姿着陆冲击内耳损伤的观察

为研究机体及各组织器官对冲击力的耐受限值，探讨致伤原因及提出防护措施，利用着陆冲击塔，使实验动物恒河猴的坐姿状态下，进行冲击试验，试验后１ｈ左右处死，作大体解剖观察并取心，肝，脾，肺，颞骨等组织，固定，切片，镜检。同时重点观察了内耳的损伤。结果表明，冲击载荷不大（平台过载值为≤２５Ｇ）时内脏虽然有轻度损伤，但内耳可避免受员，当冲击力加大到一定程度（平台过载值为３０～３５Ｇ）导致内脏中等损伤时，内耳     

腹腔放线菌病1例报告

放线菌病临床少见，而腹腔放线菌病临床更罕见。最近我们手术治疗1例腹腔放线菌病，现报告如下：患者：女性，62岁。无意中发现腹部肿块月余。体检：见中腹部膨隆，隐约可见肿块隆起，们诊触及肿块约12×10cm大小，疑累及腹壁，固定，质硬，表面尚平，无压痛，皮肤无红肿。腹部CT提示中腹部肿块，性质待查。其它检查均未见异常。拟诊腹部肿瘤剖腹探查。术中见肿块位于根结肠系膜，约6×5cm大小，质地硬，无包膜，与大网膜粘连，侵及横结肠、部分小肠、邻近腹膜及腹壁肌肉，肠系膜及腹主动脉旁淋巴结不肿大，肝脏质地正常。施行肿块连同大网…     

微波消融心室肌的实验研究——输出功率对损伤范围的影响

有形微波天线导管消融８只犬左右心室，观察输出功率对损伤范围的影响。放电时间为５０ｓ，输出功率为２０Ｗ增加到１００Ｗ。左右心室损伤体积随输出功率增加而明显扩大（Ｐ〈０．０１），损伤体积与输出功率呈正相关。在相同能量下，左右室损伤体积无明显差异（Ｐ〉０．０５）。光镜显示消融部位呈均匀一致的凝固性坏死，与周转组织界线清楚。微波消融心室肌损伤范围较射频大，可用于治疗学位     

PCR操作中的几点体会

多聚链酶反应(Polymerase chain relrion PCR)是将特异的核苷酸序列在体外进行多次扩增的一种新技术,近年来已广泛用于病原微生物检测、遗传病及肿瘤的诊断等生物医学领域。但PCR在实际应用中,仍存在一些问题,需不断总结并加以改进。     

从生物学上看耳与肺关系的密切性(耳与肺关系探讨之二)

<正> 一、祖国医学认识:关于耳与肺的关系,古圣医贤已有众多精辟的论述。(一)肺主气,司呼吸。肺气通于耳,(二)肺脉络会于耳中。(三)肺开窍于鼻,内通于耳,外合皮毛。因此可以看出:肺气通于耳,既可通过经络行于耳中,也可通过“颃颡”之分气通于耳中,故气是耳正常活动的基础。肺还通过其宣发功能将卫气和津液输布全身,温养皮毛、气门(汗孔),又可宣发肺气,气使耳、肺、皮毛间的关系更为密切。     

增殖细胞核抗原及nm23在胃癌中的表达及意义

目的 :探讨增殖细胞核抗原 (PCNA)及nm2 3基因在胃癌中的表达及其与生物学行为的关系。方法 :应用免疫组化SP法研究 85例胃癌手术标本中PCNA及nm2 3基因的表达。结果 :在 85例胃癌中PCNA、nm2 3的总阳性表达率分别为 5 2 9% (4 4 / 85例 )和 4 4 .7% (38/ 85例 ) ,PCNA及nm2 3在胃癌中的表达与其淋巴结转移、浸润深度、分化程度有关 (P <0 0 5 ,P <0 0 1 ) ;PCNA与分化程度无关 ,P >0 0 5。结论 :PCNA及nm2 3参与了胃癌的发生、发展、浸润及转移     

腓肠神经伴行血管蒂逆行岛状皮瓣的临床应用

营养血管皮瓣是小腿后部皮瓣，皮神经不是皮瓣成活的基础，主要是腓肠神经营养血管系统通过与周围多个血管网联系，形成纵贯小腿后部全长皮肤供血轴心。2000年2月-2003年5月应用腓肠神经伴行血管蒂瓣修复小腿下端、踝关节、足背创面11例。其中男7例，女4例，年龄19-67岁。其中外伤开放骨折肌腱、皮肤缺损5例；慢性骨髓炎、骨缺损2例；糖尿病导致皮肤溃烂缺损2例；钢丝绞伤1例；跟骨骨折、骨缺损继发骨浅表感染1例。     

肺切除术影响围手术期右心功能

Objective To investigate the changes of perioperative right ventricular function after pulmonary resction. Methods 45 cases of pulmonary resection were divided into four groups.Group Ⅰwas wedge resection(n = 10), GroupⅡ was lobectomy(n = 19), Group Ⅲ was double lobectomy(n=7)and Group Ⅳ was pneumonectomy(n=9). The changes of CVP, PAMP, PEP/ET, Sa, VTIs, RVSP, Ea/Aa, and Tel were evaluated by traditional ultrasonic and tissue Doppler ultrasonic techniques at different time inter- vals (pre-operation, 5-7 days orland 1 month post-operation), Results Compared with the pre-operation CVP, the indexes of all groups have no significant changes post-operatively. Wedge resection didn' t obviously influence right cardiac after load and systolic function/diastolic function. No evidet changes detected in Tei pre-and post-operatively. However, the indexes (PAMP, RVSP and PEP/ET ratio) reflecting right cardiac afterload evidently increased at post-pneumonectomy or lobectomy group5- 7 days post-opera- tive. All these reflect the decrease of Tei, which was more obvious in pneumonectomy than in lobectomy group. Right cardiac after- load, systolic/diastolic function and Tel index recovered to pre-operative level 1 month post-operation in single lobectomy group. But the above indicators, especially the Tel, were still high in double lobectomy and pmeunonectomy groups 1 morth post-operation.Tei index is positively correlated with PAMP and is weakly correlated with PET/ET ratio and Ea/Aa ratio. Conclusion Pulmonary wedge resection doesn't evidently influence right cardiac function. However, right cardiac diastolic function evidently decreases temporarily at lobectomy group. The systolic function and diastolic funetions decrease after double-lobectomy and pneumonectomy and it's more evident in pneumonectomy group. Though the right cardiac afterloads of lobectomy, double lobectomy and pneumonectomy groupa all increase significantly post-operativlye, only the former recover to pre-operative level 1 month after surgery.