下睑缘横置切口矫正内眦赘皮的术式探讨

目的 探讨下睑缘横置切口矫正内眦赘皮术式的应用.方法 2007年1月至2008年11月共为38例施行矫正术式.术中横向切开赘皮后,继沿下睑缘向外侧横行切开,皮下充分游离松解,剪除错位的眼轮匝肌纤维,内眦点定位缝合后,下睑缘切口修剪缝合.结果 38例内眦赘皮形象明显改善,大部分受术者满意,术后瘢痕位于下睑缘下和重睑线处,隐蔽性好,除1例瘢痕增生外,其余愈好良好.结论 下睑缘横置切口矫正内眦赘皮术式术后瘢痕隐蔽,效果可靠.     

跨膜接头蛋白PAG1过表达对皮肤鳞癌细胞A431运动能力的影响

目的 研究跨膜接头蛋白PAG1过表达对人皮肤鳞状细胞癌A431运动能力的影响。方法 构建PAG1-EGFP融合蛋白的慢病毒表达载体,采用反转录病毒转染的方法建立PAG1过表达的A431细胞株,实验分为3组：亲本细胞组（未进行基因转染的A431细胞）、对照组（A431细胞转染仅含EGFP阴性对照病毒）、实验组（A431细胞转染PAG1-EGFP病毒）。流式细胞术检测细胞转染率,实时荧光定量PCR及Western blot检测转染后PAG1 mRNA及其蛋白表达,进一步验证细胞转染成功与否;利用划痕修复实验、Transwell迁移实验、侵袭实验三种不同的方法检测PAG1基因的过表达是否会对皮肤鳞癌细胞的生长、迁移和侵袭能力产生影响。 结果 实验组和对照组目的基因病毒的转染表达率分别为（94.97±0.15）%、（94.60±0.35）%;实验组细胞中PAG1基因mRNA的表达量约为亲本细胞组的1.6倍（P=0.000）,转染后实验组中PAG1蛋白的相对表达水平明显增加（P=0.000）,建立了稳定过表达PAG1的A431细胞系;PAG1过表达明显降低A431细胞愈合率（P=0.000）;实验组A431细胞迁移、侵袭能力明显降低（P=0.001, P=0.000）。结论 PAG1的过表达能抑制人皮肤鳞癌细胞运动能力,影响肿瘤的浸润及远处转移。     

下睑缘横置切口矫正内眦赘皮的术式探讨

目的 探讨下睑缘横置切口矫正内眦赘皮术式的应用.方法 2007年1月至2008年11月共为38例施行矫正术式.术中横向切开赘皮后,继沿下睑缘向外侧横行切开,皮下充分游离松解,剪除错位的眼轮匝肌纤维,内眦点定位缝合后,下睑缘切口修剪缝合.结果 38例内眦赘皮形象明显改善,大部分受术者满意,术后瘢痕位于下睑缘下和重睑线处,隐蔽性好,除1例瘢痕增生外,其余愈好良好.结论 下睑缘横置切口矫正内眦赘皮术式术后瘢痕隐蔽,效果可靠.     

Comparison of the application methods and effects of skin flaps in the repair of defects in different parts of the nose according to the concept of aesthetic nosesubunits北大核心CSCD

Objective To analyze the difference of application methods and effects of local flap in small and medium‑sized defects of different aesthetic subunits of nose, in order to provide reference for clinical work. Methods A retrospective analysis was made on 59 patients with external nasal masses and scars who underwent surgical treatment in the Department of Aesthetic Plastic Surgery of the Affiliated Hospital of Qingdao University from July 1, 2021 to January 30, 2022, including 27 females and 32 males, aged 15 to 69 years. Using Likert scale, the repair methods and effects of local flap for nasal soft tissue defects were evaluated and summarized from three aspects of texture, flatness and scar concealment. GraphPad Prism 5.0 software was used for data statistics and analysis. Results The use of skin flaps to repair small and medium‑sized areas of the nose could achieve satisfactory results. For patients with different subunits, in terms of skin flatness and scar concealment degree in the operation area, patients′ satisfaction with the dorsal and lateral nasal areas was higher than that of the alar and tip areas, respectively (F=6.40, P=0.001; F=10.57, P<0.001). For patients with different skin flap repair methods, the satisfaction of patients with Z‑plasty and Dufourmentel skin flap was higher than that of other skin flap repair methods (F=4.38, P=0.002), and the satisfaction of patients with Dufourmentel skin flap was the highest in the degree of scar concealment (F=2.57, P=0.038). Conclusions In the small and medium‑sized defects of the nose, the use of multiple local flaps can achieve good cosmetic effects and functional recovery. The operator should select the appropriate flap repair method according to the characteristics of different aesthetic subunits of the nose. © 2023 Authors. All rights reserved.