红外光谱与计算机对比软件对不同生地黄的快速鉴别

目的:对野生和栽培生地黄及不同贮藏年限的生地黄(2000、2002年采收)以及"道地(河南)和非道地(其他省份)"生地黄药材进行快速鉴别研究.方法:利用傅里叶红外光谱技术(FT-IR)结合对比软件,并借助计算机运用归一化图谱辅助处理技术.结果:野生和栽培生地黄红外吸收光谱有明显的差异;对不同贮藏年限生地黄的识别率为100%;"道地和非道地"生地黄既有整体差异,又有个体交叉,识别率高于90%.结论:该方法具有快速、准确等特点.     

化痰解瘀汤联合胰岛素治疗2型糖尿病疗效观察

目的:观察血糖控制不佳的2型糖尿病患者联合使用化痰解瘀汤及胰岛素的疗效.方法:将43例空腹血糖(FPG)≥11mmol/L餐后2h血糖(2hPG)≥15mtmol/L的2型糖尿病患者分成2组:对照组继续皮注胰岛素,治疗组在胰岛素基础上加用化痰解瘀汤,共用24周,观察疗前、疗后第2、12、24周血糖、HbA1c,疗前、疗后空腹胰岛素(FINS),胰岛素抵抗指数(Homa-IR),C肽及血脂的变化.结果:两组治疗前后的空腹、餐后血糖、HbA1c均显著下降(P＜0.05),而治疗组降糖效果更为明显(P＜0.05),治疗组有降低血脂、C肽水平,减少胰岛素用量,提高胰岛素敏感性的作用,且较对照组有明显差异(P＜0.05).结论:化痰解瘀汤能降低血糖、HbA1c,减少胰岛素用量,提高胰岛素敏感性,改善胰岛素抵抗,且有降脂作用.     

中西医结合治疗慢性病毒性肝炎降低内毒素水平的临床观察

目的:观察中西医结合治疗降低慢性病毒性肝炎患者内毒素水平的临床疗效.方法:将92例患者随机分两组:治疗组和对照组,运用鲎试剂定量检测技术对病例内毒素水平采血检测.结果:治疗组内毒素水平1.64±0.46Eu/ml,至第七周恢复正常者占50%;对照组内毒素水平2.10±0.51Eu/ml,至第七周恢复正常者占30.6%;两组间疗效有显著性差异(P＜0.05).结论:中西结合治疗对降低内毒素水平有较好的疗效.     

仙人掌原毒性的初步研究

背景与目的:研究仙人掌原液的毒性。材料与方法:小鼠急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、大鼠30 d喂养试验。结果:仙人掌原液雌、雄小鼠LD50均大于20.0 g／kg,属无毒物质;Ames试验、微核试验和精子畸形试验结果均为阴性;大鼠30 d喂养试验结果显示该样品30 d喂养对大鼠各项观察指标未见毒性作用。结论:在本次实验条件下,仙人掌原液为无毒物质,未显示有遗传毒性和亚急性毒性作用。     

Results of Treating Vertebral Metastases by Percutaneous Vertebroplasty Combined with Interventional Chemotherapy

OBJECTIVE Vertebral metastases are a common manifestation in patients with advanced cancer and treatment is often ineffective. This study was conducted to explore the efficacy of treating vertebral metastases by percutaneous vertebroplasty （PVP） combined with interventional chemotherapy. METHODS Seventy-five patients with vertebral metastases （42 men, 33 women; ages 31-76 years） were divided into 2 groups： 39 cases were treated by PVP combined with chemotherapy （VPCC group）, and 36 cases were treated by PVP alone （VP group）. All procedures were guided by computed tomography （CT） scanning. The results and complications were evaluated by a questionnaire regarding pain and routine follow-up. RESULTS The response rate was significantly higher in the VPCC group than in the VP group （93.0% vs 74.4%, P〈0.05）; total response rates for the VPCC and VP groups were 25.6% and 10.3% respectively. A common complication related to VPCC was transient aggravating pain. CONCLUSION PVP may ameliorate pain, and consolidate the vertebrae of patients with vertebral metastases. Its short-term effect may be enhanced by adding drugs into the bone cement.     

Evaluation of 118 Thyroid Cancer Patients Who Underwent a Re-operation after Local Resection

OBJECTIVE To evaluate the incidence of residual thyroid cancer and cervical lymph node metastasis following a previous local resection for thyroid cancer, and to discuss methods of a reoperation.METHODS From 1994～2005, 118 patients with thyroid cancer who had previously been treated with a nodule-resection or subtotal Iobectomy in other hospitals underwent a surgical re-operation.RESULTS The incidence of residual cancer at the primary site was 38.1%. The lymph node metastasis rate at the central area was 39.8%.The rate of lymph node metastasis in patients with enlarged lymph nodes in the ipsilateral internal jugular chain was 37.5%. The rate of laryngeal recurrent nerve injury was 15.2% in other hospitals while that of the second operation in our hospital was 1.6%.CONCLUSION Nodule-resection or subtotal Iobectomy alone is not indicated for patients with thyroid cancer because of the high rate of local residual cancer. It is important to be familiar with the anatomy of the laryngeal recurrent nerve for thyroid surgery. Exploration to the central area is necessary for differentiated thyroid cancer.     

Clinical observation on acupuncture treatment of 36 cases of chronic atrophic gastritis

目的:观察针灸结合治疗慢性萎缩性胃炎的临床疗效.方法:慢性萎缩性胃炎患者36例,按中医辩证,分型施以针灸治疗.治疗前及治疗后均进行胃镜、体表胃电图、胃酸和胃蛋白酶、血中胃泌素、血中前列腺素E及胃粘膜胃泌素细胞等理化指标检查.结果:针灸治疗本病有较好的效果,显效好转率达97.2%,临床症状消失率达97.0%.治疗后可使患者血中前列腺素E等防御性因子增强,使患者从胃内低酸转向正常状态,胃动力功能增强,胃电图幅值回升,并可促使胃泌素释放,有利于胃酸的分泌.结论:针灸治疗萎缩性胃炎简便经济,无副作用,见效快,疗效肯定,可重复性强,具有一定的临床意义.     

Integrinβ1 asODN-inhibition of Cell Attachment and Invasion of the Human Gastric Cancer Cell Line SGC7901

OBJECTIVE To study the inhibition of adhesion and invasion of SGC7901cells into the ECM by integrinβ1 antisense oligodeoxynucleotide asODN.METHODS asODN and control ODN were transfected into SGC7901 cells using liposomes as vectors. The distribution of the ODN was followed by immunochemistry and changes in the expression of integrinβ1 mRNA and protein were determined by RT-PCR and FCM, respectively. The adhesion and invasion into the ECM were measured by the MTT and Boyden chamber methods, respectively.RESULTS Integrinβ1 asODN which was transfected into SGC7901 cells distributed evenly in the cytoplasm and nucleus. PCR and FCM revealed a weakened band at 489bp and a left-shift curve, respectively. Adhesion and invasion assays showed decreased activity with an inhibition rate of 54% and 76%. The extent of decrease induced by integrinβ1 asODN was larger than that caused by random control ODN (P＜0.001).CONCLUSION Transfection of integrinβ1 asODN into SGC7901 cells induced a decrease in the expression of integrinβ1 mRNA and protein,resulting in a decrease in adhesion and invasion into the ECM, with a greater effect than random control ODN.