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1.
Rapid industrial and technological development has impacted ecosystem homeostasis strongly. Arsenic is one of the most detrimental environmental toxins and its management with chelating agents remains a matter of concern due to associated adverse effects. Thus, safer and more effective alternative therapy is required to manage arsenic toxicity. Based on existing evidence, native and indigenous plant-based active biomolecules appear as a promising strategy to mitigate arsenic-induced toxicity with an acceptable safety profile. In this regard, various phytochemicals (flavonoids and stilbenoids) are considered important classes of polyphenolic compounds with antioxidant and chelation effects, which may facilitate the removal of arsenic from the body more effectively and safely with regard to conventional approaches. This review presents an overview of conventional chelating agents and the potential role of flavonoids and stilbenoids in ameliorating arsenic toxicity. This report may provide a roadmap for identifying novel prophylactic/therapeutic strategies for managing arsenic toxicity.  相似文献   
2.
《Saudi Dental Journal》2022,34(5):346-354
BackgroundLipopolysaccharides (LPS) stimulate production of inflammatory cytokines. Chrysin is flavonoid beneficial for treatment of inflammatory conditions. Bone marrow mesenchymal stem cell (BM-MSC) exosomes have regenerative ability in different tissues.ObjectiveTo assess potential role of chrysin and BM-MSC exosomes on ultra-structure, viability and function of human dermal fibroblasts-adult (HDFa) stimulated by LPS.MethodsHDFa cells were divided into: Group I: Cells received no treatment. Group II: Cells were stimulated with LPS. Group III: LPS stimulated cells were treated with chrysin. Group IV: LPS stimulated cells were treated with exosomes.ResultsAfter 48 h, ultrastructural examination of HDFa cells in Group I revealed intact plasma membrane and numerous cytoplasmic organelles. Group II displayed destructed plasma membrane and apoptotic bodies. Group III showed intact plasma membrane with loss of its integrity at some areas. Group IV demonstrated intact plasma membrane that showed fusion with exosomes at some areas. Statistical analysis of MTT represented highest mean value of cell viability% in Group IV followed by Groups III, I and II respectively. Statistical analysis of enzyme-linked immunosorbent assay (ELISA) showed the highest mean value of interleukin-1β (IL-1β) was in Group II followed by Groups III, IV and I, while highest mean values of interleukin-10 (IL-10), nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins were in Group I, followed by Groups IV, III and II respectively.ConclusionsLPS have harmful consequences on ultra-structure, viability and function of HDFa cells. BM-MSC exosomes have better regenerative action on inflamed fibroblasts in comparison to chrysin.  相似文献   
3.
Chrysin is a 5,7-dihydroxyflavone and was recently shown to potently inhibit enterovirus 71 (EV71) by suppressing viral 3C protease (3Cpro) activity. In the current study, we investigated whether chrysin also shows antiviral activity against coxsackievirus B3 (CVB3), which belongs to the same genus (Enterovirus) as EV71, and assessed its ability to prevent the resulting acute pancreatitis and myocarditis. We found that chrysin showed antiviral activity against CVB3 at 10 μM, but exhibited mild cellular cytotoxicity at 50 μM, prompting us to synthesize derivatives of chrysin to increase the antiviral activity and reduce its cytotoxicity. Among four 4-substituted benzyl derivatives derived from C(5) benzyl-protected derivatives 7, 9–11 had significant antiviral activity and showed the most potent activity against CVB3 with low cytotoxicity in Vero cells. Intraperitoneal injection of CVB3 in BALB/c mice with 1×106 TCID50 (50% tissue culture infective dose) of CVB3 induced acute pancreatitis with ablation of acinar cells and increased serum CXCL1 levels, whereas the daily administration of 9 for 5 days significantly alleviated the pancreatic inflammation and reduced the elevation in serum CXCL1 levels. Collectively, we assessed the anti-CVB3 activities of chrysin and its derivatives, and found that among 4-substituted benzyl derivatives, 9 exhibited the highest activity against CVB3 in vivo, and protected mice from CVB3-induced pancreatic damage, simultaneously lowering serum CXCL1 levels.  相似文献   
4.
Tumor-associated macrophages (TAMs) are an important cause of tumorigenesis and tumor development. M2 macrophages can promote tumor growth while M1 macrophages kill tumor cells, therefore, polarizing macrophages to achieve a functional M1 phenotype could effectively play its anti-tumor role. In the current study, we synthesized a novel chrysin derivative which is termed as ChR-TD. And we found ChR-TD might be a ligand of TLR4 that polarized the TAMs towards M1 phenotype and played its anti-tumor role. Further study indicated that ChR-TD reprogrammed the macrophages into an M1 phenotype via TLR4 activation. Moreover, ChR-TD activated TLR4/NF-κB signaling pathway and promoted the NF-κB/p65 translocated into the nuclear, leading to the activation of NF-κB and proinflammatory cytokines release. In addition, type I interferon signaling was also activated by ChR-TD, leading to the expressions of IFN-α and IFN-β and its targeted genes NOS2, MCP-1 and IP-10 were significantly increased in macrophages. Importantly, these effects were disturbed in TLR4−/− macrophages, which are constructed by using CRISPR/Cas9 system. And the molecule docking simulation further indicated that ChR-TD could bind to TLR4 and might be a ligand of TLR4. Hence, these findings suggested that ChR-TD might be a ligand of TLR4 and can be used as a potential lead compound for tumors treatment.  相似文献   
5.
目的:建立测定紫果西番莲叶中白杨素含量的方法。方法:样品经硅胶柱色谱分离纯化后进行高效液相色谱分析。色谱柱为HypersilODSC18(250mm×4.6mm,5μm),流动相为乙腈-0.1磷酸(18:82),流速为1.0mL.min-1,检测波长为220nm,进样量为10μL。结果:白杨素进样浓度在0.012~0.120mg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9980);平均加样回收率为99.41%,RSD=1.6%(n=6)。结论:该方法准确、可靠、重复性好,可用于西番莲属植物中白杨素含量的测定。  相似文献   
6.
目的: 探讨白杨黄素对牙龈卟啉单胞菌(P.g)脂多糖(P.g LPS)介导的炎症环境中人牙周膜干细胞 (hPDLSCs)成骨分化能力的影响。方法:原代培养hPDLSCs, 采用流式细胞术鉴定后取第4代细胞进行实验。采用四唑盐(MTT)试验检测不同浓度P.g LPS (1, 10, 100 和1000 ng/mL)及白杨黄素 (0.1, 0.4, 1.6, 6.25, 25, 50, 100 μmol/L) 对hPDLSCs增殖能力的影响;P.g LPS与成骨诱导剂共培养24h, 48h, 72h和96h后,活性氧 (ROS)试剂盒检测hPDLSCs 的ROS含量, 实时荧光定量聚合酶链式反应 (RT-PCR)检测hPDLSCs锰超氧化物歧化酶(MnSOD), 铜锌超氧化物歧化酶(Cu/ZnSOD)和过氧化氢酶 (CAT) mRNA的表达; P.g LPS与成骨诱导剂共培养3天, 7天, 14天后, 碱性磷酸酶(ALP)试剂盒检测hPDLSCs 的ALP活性, RT-PCR法检测成骨相关转录因子(RUNX2), 锌指结构转录因子(OSX), 碱性磷酸酯酶(ALP) 和骨钙素(OCN) mRNA表达; 25 μmol/L 白杨黄素作用hPDLSCs后, 通过ROS试剂盒检测hPDLSCs 的ROS含量, 及实时PCR法检测抗氧化因子MnSOD、Cu/ZnSOD、CAT及成骨分化基因RUNX2、OSX、OCN和ALP的mRNA表达。结果:与对照组相比, MTT结果显示1000 ng/mL P.g LPS 作用hPDLSCs 72h [(0.51±0.03) 比 (0.68±0.02), 96h后(0.62±0.06) 比 (0.97±0.07), P均<0.05]均对细胞增殖活性受到显著抑制。并且25 μmol/L 的白杨黄素对hPDLSCs 增殖活性无抑制效应 [(99.8±1.02) 比 (100±1.02) %, P>0.05). 与常规成骨诱导液培养细胞相比, P.g LPS显著增加hPDLSCs的ROS含量至 (17.3±1.34) 比(3.12±1.21) ng/ml (P<0.01), 并显著降低抗氧化因子[Cu/ZnSOD, (0.89±0.24) 比 (2.84±0.27); CAT, (1.12±0.09) 比 (2.64±0.28), P均<0.05]和成骨分化基因表达 [ALP活性, (0.94±0.11) 比 (1.25±0.14); RUNX2, (1.42±0.13) 比 (1.97±0.16); OSX (1.97 ±0.16) 比 (2.68 ±0.19); OCN (1.23±0.11) 比 (2.56±0.17), P均<0.05]; 与P.g LPS+成骨诱导液组相比, 白杨黄素显著改善P.g LPS介导hPDLSCs的氧化状态及增强其成骨分化能力[RUNX2, (1.96±0.28) 比 (1.67±0.23); OSX (2.16±0.31) 比 (1.64±0.17), P均<0.05]。结论: 白杨黄素可能对P.g LPS介导的炎症环境中人牙周膜干细胞的成骨分化能力具有促进作用。  相似文献   
7.
目的:建立蜂胶毛胶中总黄酮以及高良姜素、白杨素含量测定方法,测定不同基原毛胶中上述各指标的含量。方法:以芦丁为对照品,采用uV法测定总黄酮含量,检测波长为415nm;用HPLC同时测定高良姜素、白杨素含量,采用Welchrom C18柱,以甲醇-0.15%磷酸溶液梯度洗脱,检测波长为268nm。结果:总黄酮在0~1.248mg线性关系良好,r=0.9999(n=6),平均回收率为98.82%,RSD=1.45%。高良姜素、白杨素分别在0.089~0.4450,0.115~0.580μg线性关系良好,r均达到1.000,平均回收率分别为99.03%、99.32%,RSD分别为1.10%、1.72%。结论:该方法准确、可靠、简便易行,可用于蜂胶毛胶的定量分析,为蜂胶毛胶、其提取物以及蜂胶制品的质量评价和控制提供了依据。  相似文献   
8.
目的研究活性氧生成在8-硝基白杨素(NOC)诱导人胃癌SGC-7901细胞凋亡中的作用。方法体外培养SGC-7901细胞,采用流式细胞术分析细胞凋亡率,ELISA法检测细胞组蛋白/DNA碎片,H2DCFH-DA探针流式细胞仪检测细胞活性氧(ROS)生成。结果 NOC诱导亚G1峰(Sub-G1)细胞百分率增高和细胞组蛋白/DNA碎片增加(P<0.01),促进SGC-7901细胞活性氧生成(P<0.01)。抗氧化剂N-乙酰半胱氨酸(NAC)能有效对抗NOC诱导SGC-7901细胞凋亡作用。结论 NOC诱导SGC-7901细胞凋亡作用与促进活性氧生成相关。  相似文献   
9.
目的:检测白杨素诱导白血病细胞 HL -60凋亡及其机制。方法:分别利用 MTT 实验和 Annexin V- FITC/ PI 双染实验检测细胞增殖和凋亡。Western blot 技术检测相关通路蛋白变化。结果:白杨素可以明显诱导白血病细胞 HL -60凋亡。白杨素处理后细胞的 p - AKT 蛋白水平明显下降。结论:白杨素能够通过AKT 通路诱导 HL -60细胞凋亡。  相似文献   
10.
Chrysin exists widely in plants, honey and propolis. The anti-cancer property of chrysin has been demonstrated though the molecular mechanism is not clear. In this study, we found that pre-treatment with chrysin could promote the cell death induced by TRAIL according to the morphological changes and appearance of sub-G1 peak in four human cancer cell lines. In HCT-116 cells, the results of flow cytometry analysis showed that the percentage of sub-G1 reached (38.89 ± 3.78) % when pre-treatment of chrysin was used at 40 μM, but that was only (2.53 ± 0.10) % in the untreated group and (13.22 ± 0.20) % in TRAIL alone group. The differences between the combination and the untreated or TRAIL alone group were all significant (P < 0.05) and dose-dependent effect was obvious. Similar results were obtained in CNE1 cells. In the search of molecular mechanisms, we found that pre-treatment with chrysin could increase TRAIL-induced degradation of caspase 3, caspase 8, PARP proteins. Z-VAD-fmk, which is a pan-caspase inhibitor, could inhibit the apoptosis enhanced by the combination of chrysin and TRAIL. All data indicate that chrysin can enhance the apoptosis induced by TRAIL, and the apoptosis is caspase-dependent and related to the activation of caspase 8.  相似文献   
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