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排序方式: 共有915条查询结果,搜索用时 15 毫秒
1.
Ex vivo neutrophil function in response to three different doses of glycosylated rHuG-CSF (Lenograstim) 总被引:4,自引:0,他引:4
J. Turzanski S. P. M. Crouch J. Fletcher & A. Hunter 《British journal of haematology》1997,96(1):46-54
Granulocyte colony-stimulating factor (G-CSF) is being considered as adjuvant treatment for infections in non-neutropenic patients. Normal healthy donors were given rHuG-CSF (Lenograstim) at 2.5, 5.0 and 7.5 μg/kg subcutaneously daily for 5 d. Polymorphonuclear leucocyte (PMN) function tests were carried out on peripheral blood PMN before the first injection and at 24 h and 96 h. Circulating PMN levels were also measured at these time intervals and found to be significantly increased with all doses by 24 and 96 h. Investigation of cell surface antigen expression revealed no changes in β2 integrin (CD11b/CD18) expression. L-selectin (CD62L) expression was reduced with all doses by 96 h, this being significant with the 7.5 μg/kg dose. FcγRI (CD64) levels were significantly up-regulated with the 7.5 μg/kg dose by 96 h whereas FcγRIII (CD16) expression was found to be reduced during G-CSF treatment. Superoxide anion production was significantly increased in response to N -formylmethionylleucylphenylalanine (FMLP) and opsonized Staphylococcus epidermidis stimulation at 24 h and 96 h with the 5.0 and 7.5 μg/kg doses. The initial rate of phagocytosis (0–2 min) appeared to have increased with the 7.5 and 5.0 μg/kg doses at 96 and 24 h compared with PMN responses pretreatment, although these increases were not statistically significant. These results show that G-CSF enhances the functional responses of PMN stimulated by physiological agonists and may help in the treatment of infections. 相似文献
2.
Treatment of dyskeratosis congenita with Granulocyte Colony-Stimulating Factor and Erythropoietin 总被引:1,自引:0,他引:1
Blanche P. Alter Frank H. Gardner & Robert E. Hall 《British journal of haematology》1997,97(2):309-311
Aplastic anaemia is both frequent and difficult to manage in patients with dyskeratosis congenita (DC). We recently treated a 23-year-old male for a year with granulocyte colony-stimulating factor (G-CSF) and erythropoietin (Ep), with an excellent neutrophil response, and a transient effect on haemoglobin levels. G-CSF alone or combined with other cytokines may provide at least a partial effect in pancytopenic patients with DC. 相似文献
3.
Effects of granulocyte colony-stimulating factor on bacterial translocation due to burn wound sepsis
Orhan Yalçin Gürsel Soybir Ferda Köksoy Hakki Köse Recep Öztürk Baki Çokne§eli 《Surgery today》1997,27(2):154-158
The presence of certain defects in both cellular and humoral immunity after thermal injury has been established. Likewise,
the translocation of enteric bacteria to the mesenteric lymph nodes and to distant organs has also been observed following
serious thermal injury. The effects of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation, the small
bowel mucosa, and cecal bacterial content were investigated in a rat model of burn wound sepsis in which albino Wistar rats
were scalded over 30% of their bodies, after which the lesions were infected by 1×108 colony-forming units (cfu)Pseudomonas aeruginosa. The control group was treated with 5% dextrose solution subcutaneously starting 2 days preburn, while the treatment group
received 100μg/kg human G-CSF subcutaneously. On the 4th day post burn all animals were killed to examine the bowel and culture
of the mesenteric lymph nodes (MLN), livers, and spleens. No significant differences were observed between the groups regarding
the cecal bacterial content and small bowel; however, a difference was seen in the ratio of translocation in the MLN liver
and spleen and quantitative MLN cultures. Based on these findings, G-CSF was thus found to be significantly effective in reducing
bacterial translocation due to burn wound sepsis. 相似文献
4.
S Álvarez-Ruiz† PF Peñas† J Fernández-Herrera† J Sánchez-Pérez† J Fraga‡ A García-Díez† 《Journal of the European Academy of Dermatology and Venereology》2004,18(3):310-313
In the last years, granulocyte and granulocyte-macrophage colony-stimulating factors (G-CSF and GM-CSF) are being increasingly used and several cutaneous eruptions have been reported in relation to these treatments. In 1991 Horn et al. described three patients with maculopapular eruption that paralleled the time of infusion of GM-CSF. Two of the cases showed an increase in the number and size of macrophages in the biopsy specimen. Since then, several cases have been reported showing this histopathological alteration that has been considered characteristic of reaction to G-CSF or GM-CSF. Although maculopapular eruption with enlarged macrophages can appear after chemotherapy treatment, we have found that the presentation of this eruption after the beginning of cytokine treatment is suggestive of the involvement of G-CSF and GM-CSF in the eruption. We described eight cases of patients treated with G-CSF or GM-CSF that developed maculopapular eruptions with enlarged macrophages. 相似文献
5.
Osteoporosis in severe congenital neutropenia treated with granulocyte colony-stimulating factor 总被引:2,自引:0,他引:2
N. J. Bishop D. M. Williams J. C. Compston D. M. Stirling A. Prentice 《British journal of haematology》1995,89(4):927-928
Recombinant human granulocyte colony-stimulating factor (G-CSF) has substantially improved life expectancy for children with severe congenital neutropenia (SCN). Severe osteoporosis, reported in this population, may relate to the disease process, or be a therapeutic side-effect. This report details bone loss, quantitated absorptiometrically and histomorphometrically, in a child with SCN and vertebral collapse, and the positive response to anabolic steroid and bisphosphonate therapy. 相似文献
6.
AstudyonimprovementofexpressionofhumanG-CSFcDNAtransferredwithretroviraldouble-copyvectorGuoBaoyu(郭葆玉);ZhangSuying(张淑英);XuHui... 相似文献
7.
LEMAN YEL ILHAN TEZCAN
ZDEN SANAL FÜGEN ERSOY A IZZET BERKEL 《Pediatrics international》1996,38(3):288-290
A 3.5 year old male patient with dyskeratosis congenita (DC) presented at the age of 13 months with isolated neutropenia preceding characteristic skin findings. The average absolute neutrophil count of 500/mm3 persisted without the presence of anemia or thrombocytopenia during the follow up. Neutropenia responded to granulocyte-colony stimulating factor (G-CSF) at a dose of 10 μg/kg per day. Immunologic findings were normal as was the chromosomal stability and sister chromatid exchange. 相似文献
8.
Wing-Yen Wong Donald Williams Marilyn L. Slovak Bishan Charak Amitabha Mazumder David Snyder Darleen R. Powars Russell K. Brynes 《American journal of hematology》1993,43(2):133-138
Congenital agranulocytosis terminating in acute myelogenous leukemia has been previously reported in only two cases of adolescent males. We describe the clinical and laboratory features of a 13-year-old male with congenital agranulocytosis, treated with G-CSF with initial good neutrophil response, who subsequently developed acute myeloid leukemia. This rare complication may define a preleukemic subset of patients for whom G-CSF therapy is ineffective. The diagnostic challenges of this case are presented. 相似文献
9.
Based on animal models and limited clinical experience, there is considerable interest in use of high-dose immunosuppression followed by hemopoietic stem cell transplantation as treatment for severe rheumatoid arthritis. Because of its relatively low treatment-related mortality and morbidity, autologous transplantation is a more attractive option than allogeneic transplantation for initial clinical trials, even though anecdotal reports suggest that allogeneic transplantation has a greater likelihood of bringing about long-term disease control. The approach remains experimental with many unanswered questions such as the value and safety of high-dose therapy without transplantation, the need for T cell purging, the possible deleterious effects of post-transplant hemopoietic growth factors and the potential of mini allogeneic transplantation (a process whereby intense immunosuppression is combined with less intense myelosuppression). To achieve quick progress it is essential that clinical trials be carefully designed with all cases being reported to the Autoimmune Disease Stem Cell Project Database. 相似文献
10.
目的建市多种种属外用血间充质干细胞(MSCs)的分离培养方法,探讨其对粒细胞集落刺激因子(G—CSF)的动员反应。方法用percoll(1.131g/mL)分离多种种属外周血单个核细胞,进行贴壁培养MSCs。以G-CSF作为动员剂,培养骨髓和外周血来源的MSCs,计数动员前后的成纤维细胞集落形成单位(CFU-F)。结果对多种种属进行外周血MSCs分离培养,成功率不同。在本实验条件下.可以稳定的直接分离培养出大鼠外周血的MSCs。G—CSF动员后,骨髓来源MSCs的CFU-F数昔显著高于对照组(P〈0.01);外周血来源MSCs的CFU-F数量也显著高于对照组(P〈0.01),接近4倍。结论不同种属外周血MSCs分离培养难易不同,其直接原因是生理条什下外周血中存在的MSCs量少导致的。G—CSF可以有效地动员大鼠骨髓和外周血MSCs。 相似文献