颊脂肪垫瓣在修复口腔颌面部缺损中的应用

目的探讨颊脂肪垫瓣在修复口腔颌面部缺损的应用和效果.方法将颊脂肪垫形成一蒂在后方的颊脂肪垫组织瓣转移至缺损区,在无张力下与创缘缝合,表面轻轻用碘仿纱布缝线包压法固定.用以修复口腔颌面部的良恶性肿瘤手术或损伤留下的缺损以及口腔上颌窦瘘共18例.其中修复腭部缺损2例,上颌骨切除后缺损7例,颊黏膜癌切除后缺损3例,颊部良性肿瘤切除后缺损4例,磨牙后区黏表癌切除后缺损1例,封闭拔牙后口腔上颌窦瘘1例.结果术后10 d去除碘仿纱布,见颊脂肪垫轻度水肿,2～4周后水肿明显消退,颊脂肪垫表面逐渐上皮化,6～8周内表面完全上皮化,3个月后再生黏膜与正常口腔黏膜相似.全部病例效果满意,无感染、坏死等并发症发生.结论带蒂颊脂肪垫瓣修复口腔颌面部缺损,方法简单、易行,效果满意,值得推广.     

Validation of silver-stained nucleolar organizer regions for evaluation of invasive character of urinary bladder carcinoma in rats and mice

A series of 8 rat and 16 mouse invasive bladder carcinomas were investigated for the presence of silverstained nucleolar organizer regions (AgNORs) to clarify whether this parameter is applicable to the estimation of their invasive character. With regard to number of AgNORs per cell, neither rat nor mouse carcinomas showed any difference between invasive and noninvasive sites within the same tumor. However, the frequency of cancer cells bearing bizarre dots, irregular in size and shape, was significantly higher at sites of actual invasion. Quantitative data generated using an image analyzer revealed significantly lower values for NOR roundness and significantly larger NOR size in invasive sites than in noninvasive sites in all groups. Double staining for the proliferation marker proliferating cell nuclear antigen (PCNA) and AgNORs was performed on eight rat carcinomas and a close correlation between the two was confirmed. Thus the number of AgNORs in PCNA-positive cells was significantly greater than in PCNA-negative cells. Furthermore, a particularly strong correlation was observed for incidences of PCNA-positive cells and bizarre dots (P<0.01). The quantitative data also demonstrated significant differences in size and shape of dots. It is concluded that AgNORs have diagnostic value for the invasive character of bladder carcinomas.     

细胞荧光素酶报告基因试验受CpG岛甲基化状态影响

Objective To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. Methods The methylation statuses of CpG island of CDHI in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [pGI3-A(-73)/-C(-73)pGL3-H1/-H4]and used to transfect these cell lines. The differences between these promoter reporter vectors were analyzed by t-test. Results (1) CDH1 CpG island was unmethylated in AGS, MCF7, MKN74, and PC-3 cell lines,expressed in MCF7, MKN74, and PC-3 ,but not in AGS. Expression of CDH1 was silenced by methylation in HeLa, BGC823, A549, and RKO cell lines. (2) In the four CDH1 -unmethylated MCFT, M KN74, PC-3, and AGS cell lines ,the promoter activities of pGI3-C(-73)(as 0. 78±0. 10,0. 17±0.01,0. 11±0. 01,1.19±0. 18)were significantly higher than those of pGL3-A(-73)(as 0. 30±0. 08,0. 07±0. 01,0. 07±0. 01,0. 39±0. 04) (t values are -6. 298, -12. 349, -8. 128, -7.388, and P<0. 01). However, in the four C DH1 -methylated HeLa, BGC823, A549, and RKO cell lines, the promoter activity of pGL3-C(-73)(as 0. 09±0. 02,0. 13±0. 02,0. 05±0. 01,0. 01±0. 00) was significantly lower than that of pGL3-A(-73)(as 0. 16±0. 01,0.25±0.01,0. 11±0.03,0.03±0.00) (t valued at 5.958,11. 189,3. 661,13. 866,and P<0.05). (3) In the unmethylated MKN74 and methylated RKO cell lines, the promoter activities of pGI3-H1/-H4 were obviously and contrarily different(as 1.57±0. 23/0. 94±0. 06 and 0. 38±0. 02/0. 50±0. 04 ,t values were 4. 577 and -4. 915 ,P values were 0. 010 and 0. 003). Conclusion The methylation status of CpG island of the target gene in the tested cell lines affects the promoter activity in Reporter Assay significantly. The most active one may be the most suppressive one.     

Lindane inhibition of [35S]TBPS binding to the GABAA receptor in rat brain.

The inhibition of [³⁵S]t-butylbicyclophosphorothionate ([³⁵S]TBPS) binding to the GABA_A receptor by the insecticide γ-hexachlorocyclohexane, lindane, was studied in several brain regions and using different membrane preparation methods, both in vitro and after dosing the animals with the chemical. In the latter studies, the amount of lindane remaining in the membrane suspensions used for binding assays was determined. In vitro data showed values of IC₅₀ from 150 to 1675 nM, varying in function of the membrane preparation method used. This may account for the discrepancies in IC₅₀ values found in the literature. IC₅₀ values within the range of 150–250 nM were determined using extensively washed membranes from several brain regions, so no evidence arose for brain regional differences in the affinity of lindane for the TBPS binding site. After different schedules of acute treatment with lindane, we found a manifest relationship between the extent of the observable inhibition of [³⁵S]TBPS binding and the lindane amount remaining in the membrane suspensions used for binding assays. This relationship was in good agreement with the in vitro data, so no support for an in vivo acute regulation of the binding site was obtained.     

Morphometric study of nucleolar organizer regions in human oral normal mucosa, papilloma and squamous cell carcinoma

A morphometric study of nucleolar organizer regions (NOR) was performed to analyze their distribution, volume, number and shape in the different strata of human normal oral mucosa epithelium and papilloma and in squamous cell carcinoma employing microphotographs of silver-stained paraffin sections. The different NOR-related parameters evidenced significant differences between normal mucosa, papilloma and squamous cell carcinoma. The functional polarity of normal mucosa epithelium and of papilloma is also evidenced in terms of NOR-related parameters. The discriminative value of certain NOR parameters was demonstrated.     

老年大鼠学习行为与海马长时程突触增强和神经元核仁组织者的关系

主动回避条件反应率>80％的老年大鼠为学习好组,<30％的为学习差组。学习好组在高频短串刺激后颗粒细胞层出现海马长时程突触增强(LTP)效应:颗粒细胞核内Ag-NOR面积/胞核面积比值平均为0.154±0.0043;单个胞核内Ag-NOR平均为3.051±0.2212个,学习差组高频短串刺激引起颗粒细胞层群峰电位长时程抑制;颗粒细胞核内Ag-NOR面积/胞核面积比值平均为0.077±0.0023;单个胞核内Ag-NOR平均为1.839±0.1421个,2组间3指标均有显著差异,表明年老大鼠学习能力与LTP效应有关,学习差的老年大鼠海马齿状回颗粒细胞rDNA的转录活性较学习好的明显降低。     

扩大经额硬膜外入路切除巨大侵袭性垂体瘤

目的:探讨采用扩大的经额硬膜外入路切除巨大侵袭性垂体瘤的临床应用.方法:1997年6月至2002年3月采用该入路显微外科切除巨大侵袭性垂体瘤10例,肿瘤主要累及于鞍上、蝶筛窦及上斜坡区.结果:本组10例中,肿瘤全切6例,次全切除3例,大部分切除1例.术后发生颅神经麻痹4例,其中动眼神经障碍3例,外展神经障碍1例.全组无手术死亡.术后影像学随访6～24个月,全切除肿瘤病例未见肿瘤复发,次全切除者无明显增大.结论:该入路可充分显露鞍区肿瘤,术野开阔,对脑组织损伤小,适用于已侵犯蝶筛窦区及上斜坡的侵袭性垂体瘤.     

核仁形成区嗜银蛋白定量对结肠癌前病变的监测作用

应用银染技术,对24例结肠腺癌Ⅱ级不典型增生,30例绒毛状腺瘤,14例绒毛状腺瘤Ⅲ级不典型增生和29例高分化管状腺癌进行银染核仁形成区嗜银蛋白(Ag-NORs)定量研究,观察Ag-NORs数量、形态、大小和分布在结肠肿瘤交界性病变中的表达.提示Ag-NORs四项指标定量研究对结肠脉瘤,特别是Ⅲ级不典型增生与绒毛状腺瘤之结肠癌前病变有较好的监测作用.     

两种杀灭鼠体蚤方法的效果及其评价

为有效控制黄胸鼠体表印鼠蚤的数量,作者于1997年10月13日至11月9日,在龙陵县腊勐乡以沿鼠通道和鼠洞撒灌5/万溴氰菊酯粉剂和用8/万的溴氰菊酯水剂普通滞留喷洒,做了现场设对照区的试验。结果表明:前一种方法的灭鼠率高为95.35％,所用经费为后者的1/5,后者灭蚤率为82.18％。前一种方法比后者经济有效,建议推广应用。     

核仁形成区嗜银蛋白形态定量在结肠良恶性病变鉴别诊断中的意义

应用银染技术对114例结肠癌,95例结肠腺瘤和10例正常结肠进行核仁形成区嗜银蛋白(Ag-NORs)数量、形态、大小和分布的定量研究。结肠癌的Ag-NORs均数/核(7.04 ±2.20)明显高于结肠腺瘤(3.43±0.88,P=0.0000)和正常结肠(1.25±0.09,P=0.0000)。结肠癌的异形Ag-NORs均数/100个核(61.39土22.38)明显高于结肠腺瘤(20.74土26.61,P=0.0000)和正常结肠(0,P=0.0000)。结肠癌的小型(2um)Ag-NORs均数/100个核(483.76±242·89和)78.08±23.06)明显高于结肠腺瘤(112.00±47·23和19·49±17.47 P值均=0.0000)和正常结肠(0和0.76土1.16,P值均=0.0000)。结肠癌Ag-NORs颗粒的聚合、分散、核仁内和混合型分布(23.64％、53.20％、9.30％和13.86％)明显不同于结肠腺瘤(77.72％、16.88％、5.46％和0.04％,P值均<0.05)和正常结肠(100％、0、0和0、P值均<0.05)。本研究结果提示Ag-NORs4项定量指标有助于结肠良恶性病变的鉴别诊断。