States of Water in Hydrogels Containing with Glyceryl Methacrylate

Hydrogel materials were prepared by thermopolymerization with different content of glyceryl methacrylate and hydroxyethyl methacrylate. The different states of water in swelling hydrogels were described and studied by differential scanning calorimetry （DSC）. It was found that the hydrophilicity of GMA was stronger than HEMA, the water content and bound water of GMA hydrogel are higher than HEMA hydrogel. With the increase of GMA content, the content of free water in hydrogel increased. When GMA content was lower than 50%, the increase of GMA content also increased the content of bound water; but when GMA content was higher than 50%, the increase of GMA content decreased the content of bound water, which was caused by the chain hydrogen bond formed on the GMA chain with hydroxyl group each other.     

甲基丙烯酸缩水甘油酯与甲基丙烯酸甲酯的共聚合及竞聚率测定

以N,N-二甲基甲酰胺(DMF)为溶剂,实施了甲基丙烯酸缩水甘油酯(GMA)与甲基丙烯酸甲酯(MMA)的溶液共聚合,测定了共聚物P(GMA-co-MMA)的红外光谱(FT-IR),对其化学结构进行了表征,并采用差示扫描量热法(DSC)测定了共聚物的玻璃化转变温度(Tg).改变两单体投料比进行共聚合,采用化学分析法测定低转化率下(<7%)共聚物组成,重点研究了两单体的竞聚率.结果表明:GMA与MMA的共聚合易于进行,P(GMA-co-MMA)的玻璃化转变温度(Tg)介于均聚物PGMA(72℃)与PMMA(106℃)之间,当n(GMA)/n(MMA)=4/6时,共聚物的Tg为9l℃.采用FR和KT 2种作图法及YBR计算法对单体的竞聚率进行了计算和比较,结果表明:KT和YBR法较为准确,以DMF为溶剂时,GMA与MMA的竞聚率分别为2.14与0.69.     

牙科纳米氧化铝复合树脂的制备与性能研究

目的 制备一种新型的光固化纳米氧化铝复合树脂,探讨其用于口腔临床的可行性。方法 以双酚A双甲基丙烯酸缩水甘油酯(Bis-GMA)为树脂基质,甲基丙烯酸羟乙酯(HEMA)为活性稀释剂,添加纳米氧化铝填料对树脂基质进行增强增韧改性,制备一种新型牙科纳米氧化铝复合树脂,并表征其固化程度、弯曲强度、硬度、断面形貌、耐磨性、吸水性与水溶解性。结果 添加纳米氧化铝能提高复合树脂材料的刚性和硬度,当添加量达到3wt%时,复合树脂的力学性能、吸水和溶解性能均为最优。结论 复合树脂中加入一定比例的纳米氧化铝可达到增韧和耐磨的效果,该研究为开发新型牙科复合树脂提供了理论和实验基础。     

聚合物微球固载N-羟基邻苯二甲酰亚胺催化剂的制备及其催化氧化性能初探

首先采用悬浮聚合法,制备了甲基丙烯酸缩水甘油酯(GMA)和甲基丙烯酸甲酯(MMA)的交联聚合物微球(GMA/MMA),然后经过几步大分子反应在微球表面合成与固载了N-羟基邻苯二甲酰亚胺(NHPI),形成固载有NHPI的功能微球GMA/MMA-NHPI。采用红外光谱(FT-IR)及扫描电子显微镜(SEM)等方法对功能微球进行了表征。结果表明,以含环氧基团的GMA/MMA为载体,通过大分子反应可实现NHPI的合成与固载。GMA/MMA-NHPI与醋酸钴（Co(OAc)2）构成的共催化体系,在分子氧对环己烷和环己醇的氧化过程中,显示出良好的催化活性。     

SBS-g-GMA对PPO/PBT合金性能影响的研究

采用熔融共混法制备了SBS—g-GMA/PPO/PBT、SBS/PPO/PBT的合金,通过HAAKE、MI、DSC、SEM等方法对合金进行了表征。研究了SBS—g—GMA对PPO/PBT合全相容性的影响,探讨了合全的流变性能、热性能、形态结构和力学性能。     

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

Objective To establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA) and define the different methylation genes at different stages.Methods DNA was extracted at different 16 HBE malignant phases and changes of genes DNA methylation at different stages were detected using Methylation chip of 'NimbleGen HG18 CpG Promoter Microarray Methylation'.Methylation-specific PCR(MSP) was used to observe the methylation status of some genes,and then compared with the control groups.Results The result showed that GMA induced 16 HBE morphorlogical transformation at the dose of8 μg/mL,and cell exposed to GMA had 1 374 genes in protophase,825 genes in metaphase,1 149 genes in anaphase,respectively;30 genes are all methylation in the 3 stages;318 genes in protophase but not in metaphase and anaphase;272 genes in metaphase but not in protophase and anaphase;683 genes in anaphase but not in metaphase and protophase;73 genes in protophase and metaphase but not in anaphase;67 genes in protophase and anaphase but not in metaphase;59 genes in metaphase and anaphase but not in protophase.Conclusion The pattern of DNA methylation could change in the process of 16 HBE induced by GMA.     

Composite glycidyl methacrylated dextran （Dex-GMA）/gelatin nanoparticles for localized protein delivery

Aim： Localized delivery of growth factors has significant potential as a future therapeutic strategy in tissue engineering and regenerative medicine. A nanoparticle vehicle was created and evaluated in this study with the intent to deliver growth factors for periodontal regeneration.
Methods： Novel composite nanoparticles based on glycidyl methacrylate derivatized dextrans （Dex-GMA） and gelatin were fabricated by a facile method without using any organic solvents. The configurations of the resultant nanoparticles were evaluated by transmission electron microscopy, scanning electron microscopy, and atomic force microscope. Their surfaces were characterized by zeta-potential measurements, after which their properties including swelling, degradation, drug release, and cytotoxicity were also investigated using in vitro models. Results： The particle size of Dex-GMA/gelatin nanoparticles （DG-NPs） ranged from 20 to 100 nm and showed a mono-dis- perse size distribution （mean diameter 53.7 nm） and a strongly negative surface zeta potential （-20 mV）. The DG-NPs were characterized by good swelling and degradation properties in media including dextranase. The in vitro drug release studies showed that the efficient bone morphogenetic protein （BMP） release from DG-NPs was maintained for more than 12 d under degradation conditions, where more than 90% of the loaded BMP was released. No any relevant cell damage caused by DG-NPs was found in the cytotoxicity tests for a period of 24 h.
Conclusion： These combined results demonstrate that DG-NPs fulfill the basic prerequisites for growth factor delivery. With further in vivo studies, those nanoparticles may offer a promising vehicle for the delivery of active drugs to the perio- dontium.     

右旋糖酐-甲基丙烯酸缩水甘油酯载BMP_2凝胶微球的制备与评价

目的:探讨利用右旋糖酐-甲基丙烯酸缩水甘油酯(DEX-GMA)制备载骨形态发生蛋白2(BMP2)凝胶微球的可行性和制备工艺,并初步探讨其载药、释药特性。方法:以反应温度、DEX-GMA和乳化剂Span-80的加入量和搅拌速率等为考察因素进行正交试验,筛选BMP2-DEX-GMA凝胶微球的最佳制备工艺并对成品性能进行初步检测。结果:经悬浮聚合法可以制备BMP2-DEX-GMA凝胶微球,优选出的制备工艺条件为反应温度30℃、DEX-GMA用量0.6%、Span-80用量0.06%、搅拌速率300r/min,由此制备的BMP2-DEX-GMA凝胶微球形态良好,粒径在20μm～50μm之间,包封率为(78.52±4.34)%,载药量为(10.68±1.34)%,溶胀率为85.9%,稳定性和再分散性良好。结论:该凝胶微球载药系统制备工艺简单,载药量大,可以负载具有生物活性的药物。     

纤维用耐久性抗静电剂及其性能研究

以烷基酚聚氧乙烯醚甲基丙烯酸酯、甲基丙烯酸缩水甘油酯为原料,通过溶液聚合合成一种纤维用 耐久性抗静电剂。通过正交试验确定了最佳合成工艺条件,并以扫描电镜观察抗静电剂在纤维表面的涂敷效 果。结果表明,抗静电剂能均匀地涂敷在纤维表面。抗静电剂含量为0.6 g／100 g纤维的体积电阻率达到10R Ω·cm,经多次水洗后纤维仍具有较好的抗静电性能(体积电阻率<109Ω·cm)。     

树脂粘接剂在全瓷修复中的应用

全瓷修复是当今口腔固定修复的发展趋势之一。全瓷修复体依赖良好的树脂粘接剂以保征其临床成功。目前，全瓷修复树脂粘接剂与才本质的粘接技术及瓷表面处理技术仍在不断探索改进之中，以期进一步提高临床粘接效果。本文对全瓷修复树脂粘接剂的组成及其与牙本质的粘接、与全瓷修复体的粘接原理、技术和效果作一综述。