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1.
Chihaya Maesawa Gen Tamura Yasushi Suzuki Kaoru Ishida Kazuyoshi Saito Ryoichi Satodate 《Cancer science》1992,83(12):1253-1256
For the rapid and sensitive detection of p53 gene mutations in esophageal endoscopic biopsy specimens, we combined cell sorting with the polymerase chain reaction and single-strand conformation polymorphism (PCR-SSCP) analysis. Mutations in exons 5–8 of the p53 gene were investigated by FCR-SSCF analysis using 103 sorted nuclei obtained from each endoscopic biopsy specimen of 16 patients with esophageal cancer. DNAs extracted from their respective surgical specimens were investigated by a conventional method of PCR-SSCP analysis. Mutations in the biopsy specimens were detected in 6 of the 12 aneuploid tumors but in none of the 4 diploid tumors. After tumor cell enrichment by cell sorting, one mutation in exon 8 became apparent, which could not be detected from the surgical specimen by a conventional method of PCR-SSCP analysis. This method should improve the sensitivity of detecting p53 gene mutations, and provides additional information concerning the DNA ploidy pattern in the tumors. 相似文献
2.
聚合酶链反应单链构象多态性检测乳腺癌中P53基因突变 总被引:4,自引:0,他引:4
应用聚合酶链反应单链构象多态性方法,对24例原发性乳腺癌肿瘤组织基因组DNA进行了分析,结果表明:其中7例存在P53基因的突变,突变频率约为30%。同时对其中10例进行Souternblot分析,有2例在chr17P上存在等位基因的缺失,而其另一等位基因上均存在基因突变。 相似文献
3.
Sarcomas were induced in Fl mice between C57BL/6N and C3H/He strains by subcutaneous injection of methylcholanthrene. The c-myc oncogene was found to be amplified in 16 cases among 43 sarcomas of C57BL/6N × C3H/He mice and 1 case among 5 sarcomas of the reciprocal cross. The origin of the amplified allele was determined by the polymerase chain reaction single strand conformation polymorphism analysis. Among the 17 sarcomas, only one had both of the alleles amplified. The rest of the tumors carried the amplified c-myc allele coming either from C57BL/6N (9 cases) or from C3H/He (8 cases). These results indicate that the c-myc allele is amplified randomly in methylcholanthrene-induced mouse sarcomas irrespective of its origin, such as paternal or maternal allele and C57BL/6N or C3H/He allele. In addition to these changes, the unamplified c-myc oncogene was found to be lost in 12 cases out of the 17 sarcomas with the amplification. 相似文献
4.
检测25例脑肿瘤中抑制癌基因P53突变情况。突用复合PCR,聚合酶链反应-=单链构象多态性分析法,统计学处理采用X^2检验。结果:P53基因总的突变率为44.0%,其中第5外显子突变率为28.0%,第6外显子突变率12.0%,第7外显子突变率为12.0%,第8外显子突变率16.0%;病理分级Ⅱ级及其以上突变为72.7%, 相似文献
5.
Schmid KW Bankfalvi A Mucke S Ofner D Riehemann K Schroder S Stucker A Totsch M Dockhorn-Dworniczak B 《Endocrine pathology》1996,7(2):121-130
Routinely processed tissues from a series of benign and malignant thyroid lesions were immunohistochemically investigated
with antibodies against p53 and mdm-2. p53 was immunolocalized in <10% of nuclei in 2/80 nodular goiters, 2/60 follicular
adenomas, 26/68 follicular carcinomas, 7/40 papillary carcinomas, 3/10 “insular” carcinomas, and 10/31 anaplastic carcinomas.
More than 10% positively stained nuclei were found in 2 widely invasive follicular, 2 insular, and 15 anaplastic carcinomas.
All p53-positive cases showed a concomitant immunohistochemical mdm-2 expression; an immunohistochemical colocalization on
serial section was demonstrated in 12 anaplastic carcinomas. Screening by polymerase chain reaction single-strand conformation
polymorphism (PCR-SSCP) analysis of these 12 cases revealed no relevant mutations in the coding regions of exons 2–11 of the
p53 gene. Additionally, 1 follicular adenoma, 6 follicular carcinomas (4 minimally and 2 widely invasive), 1 papillary, and
2 poorly differentiated insular carcinomas were mdm-2 positive without immunohistochemically detectable p53 expression. These
results provide evidence that wild-type p53 expression in thyroid carcinomas may be associated with mdm-2 induced formation
of stable complexes. However, the role of p53 mutations and p53 protein inactivation owing to other factors (e.g., mdm-2)
in the progression of thyroid carcinomas is still poorly understood. 相似文献
6.
哮喘人群STAT6基因多态性的研究 总被引:3,自引:0,他引:3
目的 研究信号转导和转录激活因子6(STAT6)基因3’非翻译区G2964A位点多态性与重庆哮喘人群的易感性及血浆IgE水平的关系。方法用聚合酶链反应和单链构像多态性(PCR-SSCP)的方法对42例哮喘患者及42例对照进行了STAT6基因G2964A位点多态性分析。结果哮喘组与对照组STAT6基因G2964A位点的基因型频率之间无显著性差异(P〉0.05),并且哮喘组各基因型之间均与血浆IgE升高无确定关系(P〉0.05)。结论G2964A位点多态性与重庆人群哮喘易感性可能无明显相关性。 相似文献
7.
J. A. J. M. van den Hurk P. M. van Zandvoort F. Brunsmann I. H. Pawlowitzki W. Holzgreve P. Szabo F. P. M. Cremers B. A. van Oost 《American journal of medical genetics. Part A》1992,44(6):822-823
We performed prenatal testing to predict the inheritance of choroideremia (CHM) using a linked polymorphic DNA marker, DXS95. DNA analysis of chorionic villi at the 12th week of pregnancy indicated that the allele at risk had not been passed from the heterozygous mother to the fetus. This prenatal exclusion of choroideremia was confirmed by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. © 1992 Wiley-Liss, Inc. 相似文献
8.
Alleles encoding HLA-C antigens in Japanese were identified by polymerase chain reaction followed by single strand conformation polymorphism (PCR-SSCP) and nucleotide sequencing analyses. The results showed that at least sixteen different alleles code for eight serologically detectable antigen groups and undetectable blanks. Cwl was mainly encoded by Cw*0102, whereas two split antigens of Cw3, Cw9 and Cw10, were encoded by Cw*0303 and Cw*0304, respectively. Cw4 and Cw6 were encoded by Cw*0401 and Cw*0602, respectively. Seven alleles, Cw*0801, Cw*0803, Cw*1202, Cw*1203, Cw*1402, Cw*1403 and Cw*1502, were found to encode serological HLA-C "blanks" in Japanese. Moreover, errors in the published nucleotide sequences of Cw*0501 and Cw*1201 were corrected. Twenty-one HLA-C alleles were distinguished from each other by means of group-specific PCR amplification followed by the SSCP method developed in the present study. The system using genomic DNAs can be used effectively for identification of new HLA-C alleles. 相似文献
9.
Abstract: The dog has been an important model for solid organ and hematopoietic stem cell transplantation for over 30 years. Fundamental to the continuing usage of the model is the development of molecular-based histocompatibility typing of donors and recipients. Previous histocompatibility typing methods used in the dog have not been precise enough to identify dog leukocyte antigen (DLA)-matched unrelated dogs. This study was undertaken to begin the process of identifying DLA-matched unrelated dogs. In this study polymerase chain reaction-single-stranded conformational polymorphism is used to separate alleles thereby allowing sequenced-based typing of the two most polymorphic class II genes described to date in the dog - DLA-DRB1 and DLA-DQB1. 相似文献
10.
We have investigated the frequencies of HLA-B*07 alleles and their haplotypic associations with HLA-A, -C and -DRB1 loci in 489 healthy unrelated Koreans, including 214 parents from 107 families. All of the 45 samples (9.2%) typed as B7 by serology were analyzed for B*07 alleles using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) method. Two different B*07 alleles were detected: B*0702 (allele frequency 0.041) and B*0705 (0.005). Two characteristic haplotypes showing strong linkage disequilibrium in Koreans were A*2402-Cw*07-B*0702-DRB1*0101 (haplotype frequency 0.028) and A*2901-B*0705-DRB1*0803 (0.005). The characteristic haplotype A*2901-B*0705-DRB1*0803, found in 100% (5/5) of B*0705-positive individuals, has not been previously described in other ethnic groups. HLA-B7 alleles comprise distinctive extended haplotypes in the Korean population. The probability of HLA-B7 allele mismatches among ABDR-matched unrelated donor-recipient pairs is expected to be low in Koreans. 相似文献