中药五倍子对不同根面龋损提取非胶原蛋白后再矿化的影响

目的体外观察人工形成的牛牙根面龋提取非胶原蛋白前后,中药五倍子对人工龋再矿化的影响。方法体外37℃恒温箱中,两种脱矿溶液中用牛牙模拟人牙形成不同形态根面龋模型(酸蚀性病损和表层下脱矿)。每组随机选择两个样本用偏光显微镜(PLM)观察表面形态;用盐溶液提取根面龋暴露区的可溶性牙本质磷蛋白(S-DPP),在恒温箱中用五倍子多酚化合物(GCE)对提取非胶原蛋白前后的根面龋同时进行再矿化循环7d,用激光共聚焦扫描显微镜(LSCM)观察样本并分析平均荧光量。结果 PLM观察显示表层下脱矿形成了较完好的表层,而酸蚀性病损则无表层形成。LSCM分析结果显示在酸蚀性病损形态下提取S-DPP后GCE处理荧光强度明显下降(P<0.05),而在表层下脱矿形态下提取S-DPP前后GCE处理荧光强度无明显差异(P>0.05)。结论在酸蚀性病损条件下提取S-DDP后GCE可能有更高的再矿化效果。     

硅酸三钙阻塞牙本质小管的实验研究

目的：评价硅酸三钙对牙本质小管的阻塞能力。方法：从无龋的离体第三磨牙切取牙本质片,用30%磷酸酸蚀1min来模拟牙本质过敏的状态,然后牙本质片表面用硅酸三钙糊剂涂抹和刷洗,之后浸入人工唾液中7d。结果：扫描电镜显示牙本质小管口几乎都被纳米晶体所覆盖;通过EDS和XRD证实该纳米晶为羟基磷灰石。结论：硅酸三钙不仅通过自固化过程阻塞牙本质小管,而且在生理环境下能诱导牙本质再矿化。因此,可考虑用作治疗牙本质过敏的有效药物。     

Effects of fluoridated milk on artificial enamel carious lesions: a pH cycling study

Objective

To investigate the relationship between concentration of fluoride in milk and its re-mineralizing efficacy, and to test whether increasing the volume of fluoridated milk has any effect on its re-mineralizing efficacy.

Methods

Third molars were painted leaving a 1 mm window on the buccal and/or lingual surfaces. Artificial carious lesions were induced using a de-mineralizing solution. The teeth were then sectioned longitudinally. Specimens were randomly assigned to 15 groups according to different concentrations and volume; i.e. 2.5 ppm, 5 ppm, 7.5 ppm, 10 ppm, 15 ppm, 20 ppm, and 250 ppm of fluoride with 25 ml/section and 50 ml/section. De-ionized water and plain milk were used as controls. Sections were subjected to pH cycling for 20 days. Polarized light microscopy and micro-radiography were utilized to record the lesion characteristics before and after pH cycling.

Results

Mean lesion depths decreased significantly in all the fluoride groups, and increased significantly in the control groups. There were no differences in percentage of lesion depth decrease when the volume was doubled for the same fluoride concentration. There were also no statistically significant differences between percentages of decrease in lesion depth when the concentration of fluoride in milk was increased.

Conclusion

Fluoridated milk demonstrated a remineralization efficacy on early enamel carious lesions. A 2.5 ppm fluoride milk concentration provided similar remineralization potential to that of higher fluoride concentration in milk.     

CPP-ACP治疗正畸后釉质白斑的临床疗效评价

目的 评价酪蛋白磷酸多肽-无定型磷酸钙(casein phosphopeptide-amorphic calcium phosphate,CPP-ACP,GC护牙素)对正畸治疗所致牙釉质白斑再矿化的临床疗效.方法 选取正畸治疗结束后多颗牙出现釉质白斑的患者20例,随机分为安慰剂组(A组)和GC护牙素组(B组),每组10例.拍摄治疗前和治疗后的牙张照片,观察牙釉质白斑的变化情况,计算釉质脱矿指数(EDI),并进行评价.结果 两组治疗后EDI均低于治疗前,差异有统计学意义(P＜0.001).B组治疗前后EDI差值大于A组,两组相比差异有统计学意义(P＜0.001).结论 CPP-ACP对正畸所致牙釉质白斑的再矿化治疗有效.     

渗透树脂联合生物活性玻璃对早期脱矿牙釉质表面显微硬度及再矿化的影响

目的 探究渗透树脂联合生物活性玻璃对早期脱矿牙釉质表面显微硬度及对再矿化的影响。方法 选取2021年6月至2022年4月本院收治的80例正畸治疗患者，共取120颗牙齿，随机数字表法分为空白对照组（A组）、渗透树脂组（B组）、渗透树脂联合氟化钠组（C组）及渗透树脂联合生物活性玻璃组（D组），每组30颗。比较处理前、脱矿后、再矿化处理后及再脱矿处理后牙釉质表面粗糙度及牙釉质显微硬度、脱矿24 h、48 h和72 h后钙容量测定及所有区域内荧光量面积（A）、总荧光量（IAF）、平均荧光密度（AF）。结果 再矿化和再脱矿处理后组间比较粗糙度值为A组（4.73±0.70μm）>B组（2.87±0.05μm）>C组（1.52±0.09μm）>D组（0.25±0.03μm）]（P<0.05）；再矿化和再脱矿处理后组间比较牙釉质表面硬度（HV）值为A组（24.74±4.28）P<0.05）；再脱矿24 h、48 h和72 h后的钙溶出总量D组P<0.05）；A组、B组、C组的A和TF显著低于D组（P<0.05），各组间的AF值比较差异无统计学意义（P>0.05）。结论 渗透树脂联合生物活性玻璃可提高早期脱矿牙釉质表面显微硬度，且脱矿效果及抗脱矿能力效果良好，具有临床推广应用价值。