转染反义磷脂酶A2寡核苷酸抑制LPS诱导Hela细胞分泌IL-1β和IL-6

目的：研究脑浆型磷脂酶A2(cytosolic phospholipase A2,cPLA2,85—kD PLA2)在脂多糖诱导Hela细胞释放细胞因子IL-1β、IL-6的信号机制中的作用。方法：①利用脂质体将cPLA2的反义寡核苷酸转染入Hela细胞，通过聚合酶链式反应(RT—PCR)及Western杂交印迹分析检验胞浆中cPLA2人水平的变化；②用放免方法检测不同时间培养上清中的IL-1β、IL-6浓度。结果：①转染后cPLA2的蛋白表达明显减少，但mRNA水平未见显著改变；②Hela细胞释放IL-1β、IL-6随cPLA2人反义寡核苷酸浓度的增加而减少。结论：cPLA2人LPS刺激的Hela细胞释放炎性细胞因子IL-1β、IL-6的过程中可能发挥重要的信号传递作用。     

Comparison of Three Screening Methods for Sarcopenia in Community-Dwelling Older Persons

ObjectiveThe 2019 Asian Working Group on Sarcopenia in Older People (AWGS 2019) recommends using either calf circumference or the strength, assistance in walking, rising from a chair, climbing stairs, and falls (SARC-F) or SARC-F combined with calf circumference (SARC-CalF) questionnaires for sarcopenia screening. The aim of this study was to compare the ability and applicability of calf circumference, SARC-F, and SARC-CalF for screening sarcopenia among community-dwelling older adults.DesignCross-sectional study.Setting and ParticipantsA total of 1050 community-dwelling older people were enrolled.MethodsSarcopenia was diagnosed according to the AWGS 2019 criteria: bioimpedance analysis for appendicular skeletal muscle index, hand grip, and 6-m gait speed test. Participants also completed the SARC-F questionnaire and calf circumference measurement. The screening tools’ performances were evaluated through receiver operating characteristic (ROC) curves, area under the ROC curves (AUC), and sensitivity/specificity analyses.ResultsSarcopenia was identified in 263 (25.0%) participants by the AWGS 2019 criteria. Calf circumference had a sensitivity of 81.4% and a specificity of 77.0%. Sensitivity and specificity of SARC-F for screening sarcopenia were 17.9% and 93.7%, respectively. SARC-CalF improved the sensitivity of SARC-F (47.5%) while keeping similar specificity (92.0%). The AUCs of calf circumference, SARC-F, and SARC-CalF were 0.79 [95% confidence interval (CI), 0.77–0.82], 0.56 (95% CI, 0.52–0.59), and 0.70 (95% CI, 0.67–0.73), respectively. The differences across ROC curves were statistically significant among 3 screening tools (P < .001).Conclusions and ImplicationsThe overall screening ability of calf circumference was better than that of SARC-F and SARC-CalF for sarcopenia in community-dwelling older persons despite gender, age, and cognitive function. SARC-F and SARC-CalF have high specificity but are susceptible to the preceding factors.     

酮洛芬的相转移催化氧化合成

2-(3-苄基苯基)丙醛和高锰酸钾在相转移催化剂度米芬作用下,以苯为有机相、氢氧化钠水溶液为水相氧化制得酮洛芬,收率69%,纯度99.5%.     

Effects of dietary supplementation with vitamin E and selenium on rat hepatic stellate cell apoptosis

AIM: To evaluate the effects of dietary supplementation with vitamin E and selenium on proliferation and apoptosis of hepatic stellate cells (HSCs), in acute liver injury induced by CCl4, and to explore their role in the recovery from hepatic fibrosis phase. METHODS: An acute liver damage model of rats was established by intraperitoneal injection of carbon tetrachloride (0.3 mL/100 g body weight) twice a week, then the rats were killed at 6, 24, 48, and 72 h after the first and third injection, respectively. A liver fibrosis model was established by the same injection for 8 wk. Then three rats were killed at 3, 7,14, and 28 d after the last injection, respectively. The rats from the intervention group were fed with chow supplemented with vitamin E (250 mg/kg) and selenium (0.2 mg/kg), and the rats in the normal control group and pathological group were given standard chow. Livers were harvested and stained with hematoxylin and eosin, Sirius red. Activated HSCs were determined by α-smooth muscle actin immunohistochemistry staining. Apoptotic HSCs were determined by dual staining with the terminal deoxynucleotidyl transferase UTP nick end labeling (TUNEL) and α-smooth muscle actin immunohistochemistry. Serum alanine aminotransferase and aspartate aminotransferase were also analyzed. RESULTS: In the acute liver damage model, the degree of liver injury was more serious in the pathological group than in the intervention group. At each time point, the number of activated HSCs was less in the intervention group than in the pathological group, while the number of apoptotic HSCs was more in the intervention group than in the pathological group. In the liver fibrosis model, the degree of liver fibrosis was more serious in the pathological group than in the intervention group. At each time point, the number of activated HSCs was less in the intervention group than in the pathological group, and the number of apoptotic HSCs was more in the intervention group than in the pathological group. CONCLUSION: Vitamin E and selenium supplementation at the given level can inhibit CCl4-induced activation and proliferation of HSCs and promote the apoptosis of activated HSCs in acute damage phase. Vitamin E and selenium can also effectively decrease the degree of hepatic fibrosis and promote the recovery process.     

人工血管移植动静脉瘘术后超声检测价值

目的 探讨彩色多普勒超声对人工血管移植动静脉瘘及其并发症的检测价值.方法 对18例人工血管移植动静脉瘘术后4～6周的患者,应用彩色多普勒超声测量动脉侧吻合口和移植人工血管动脉端内径、峰值流速和血流量,同时对上肢出现不适症状患者进行超声检测.结果 术后4～6周彩色多普勒超声显示吻合口和移植人工血管血流充盈良好,动脉侧吻合口内径、峰值流速和血流量分别为(3.61±0.68)mm、(298.56±93.42)cm/s、(583.62±216.77) ml/min;人工血管动脉端内径、峰值流速和血流量分别为(4.47±0.61)mm、(219.37±68.42)cm/s、(325.23±117.12)ml/min.7例患者手术侧上肢出现不适症状,超声检查发现1例血清肿;3例移植血管血栓;1例假性动脉瘤;2例单纯性皮下组织水肿.结论 超声对人工血管移植动静脉瘘术后人工血管及其并发症的检测有重要的临床应用价值.     

血清GPDA测定及其在肿瘤检查中的应用

测定６０例健康中、老年人血清ＧＰＤＡ活力并与１００例经确诊的肝疾患和恶性肿瘤患者进行比较。结果表明：食管、胃、肠道的肿瘤血清ＧＰＤＡ呈一致性减低，而肝硬化、肝癌、胆道、胰、肺、乳腺癌则是一致性升高。初步认为其升高或降低的机制与组织发生学有关。血清ＧＰＤＡ测定对恶性肿瘤的发生有一定的诊断意义，对肿瘤的发生部位有一定的鉴别价值。     

Establishment of a highly sensitive leptin radioimmunoassay and detection of increased leptin levels in hyperlipidemia and pregnancy

The highly effective antibody has been obtained by immunizing rabbits with recombinant leptin many times. The leptin is iodinated with the chloramine-T method and purified with a Sephadex-G25 chromatography column. The reaction between antigen and antibody is carried out by a one-step balance method and cultured at 4 degrees C for 24 h; the binding and free antigen was then separated by PR reagent. The determining range of this method is about 0.5-24 ng/mL; limited detection level is 0.45 ng/mL, relative standard deviation in a group, and among groups, are less than 5.4% and 8%, respectively. The level of blood leptin in 277 samples of normal persons, in 112 samples of overweight persons (weight/hieght m2 > or = 25) and 224 samples of hyperlipidemic patients have been measured by this method. It is demonstrated that the level of blood leptin in males is much lower than that of the females, and becomes elevated with increased age. Serum leptin level in overweight persons and hyperlipidemic patients is also much higher than that of normal groups (P < 0.01). Serum leptin of 21 workers in our lab at 8:00 AM and 4:00 PM has been tested. It was found that there are no differences between the two time points. The same results are obtained within age groups. Leptin levels of pregnant women's serum is higher than those of the control group (P < 0.001). Leptin in newborn's serum is significantly lower than those of mothers (P < 0.01). There is no obvious correlation between leptin level of mother and newborns by correlation analysis (r = 0.19, P > 0.05). The body weight and body weight index of pregnant women are well correlated with their serum leptin levels (r = 0.33 and 0.35, P < 0.05). The body weight and body weight index of newborns are well correlated with their serum leptin levels (r = 0.54 and 0.49, P < 0.001). The serum leptin level of pregnant women is not correlated with newborn's body weight (r = 0.10). These results have shown that the proposed method is stable, simple, and specific, being sensitive enough to determine leptin levels in human serum or plasma.     

Interstitial chemotherapy with ricin-loaded thermosensitive hydrogel in pancreatic cancer xenograft

BACKGROUND:Pancreatic cancer is one of the most aggressive malignancies,and has a poor prognosis. Despite efforts made in multiple fields,there has been little success in improving the disease-free survival rate of patients.This study was undertaken to investigate the effectiveness and feasibility of using intra-tumoral injection of ricin-loaded thermosensitive hydrogel for treatment of pancreatic cancer xenografts,attempting to develop a new treatment for human pancreatic cancer. METHODS:BALB/c-(nu/nu)nude...     

脑活素改善血管性痴呆患者智能状况的临床研究

目的:探索脑活素对血管性痴呆患者智能状况的改善效果。方法:将29例符合血管性痴呆标准的患者分为对照组和治疗组。对照组用血塞通0.4g,治疗组在对照组的基础上加用脑活素20mL,10d为1个疗程,3年共6个疗程,其后分别采用中文版简易智能状态检查(MMSE)量表进行积分,用中国人修订韦氏成人量表(WAIS-RC)进行智力测验比较。结果:根据WAIS-RC测验结果,两组3年后知识(11.32±1.76比9.23±1.78)、领悟(13.19±1.85比11.24±2.12)、算术(10.18±1.32比8.63±1.25)、相似(9.46±0.68比7.56±0.98)、数字广度(10.12±0.69比8.68±0.97)、词汇(31.25±0.78比27.32±1.02)、数字符号(31.35±1.20比25.35±0.78)、填图(9.78±0.98比7.45±0.65)、图片排列(19.16±0.74比15.23±1.52)等项目比较,治疗组积分明显高于对照组(t=1.96～5.21,P<0.05或0.01)。治疗组治疗前后上述项目自身对照积分亦有显著提高(t=1.96～5.21,P<0.05或0.01)。结论:脑活素能有效抑制神经细胞的凋亡,减少脑功能的受累,治疗血管性痴呆,对老年群体的康复有一定的治疗作用。