白细胞介素5的IgA表达增强作用

白细胞介素5(IL-5)可促使派伊尔氏淋巴集结(PP)及脾脏B 细胞合成IgA 增加。IL-4协同IL-5增加IgA 的表达。T 辅助细胞2(T_H2)克隆及其培养上清增加IgA 表达是IL-5和IL-4的共同作用所致。IL-5的作用机制是促使膜表面IgA 阳性(mIgA~+)B 细胞分化为分泌IgA 的浆细胞及增加所谓大B 细胞表达IgA。IL-4则可促使膜表面IgM 阳性(mIgM~+)B 细胞分化为mIgA~+B 细胞而协同IL-5增强IgA 的表达。     

胶乳凝集试验检测鼠型斑诊伤寒抗体

A latex test for immunodiagnosis of murine typhus, using erythrocyte-sensitizing substance from Rickettsia prowazekii absorbed to latex particles, has been developed. The test was evaluated with a total of 234 healthy person and non-typhus patient sera, 89 single and 63 paired typhus patient sera. The positive rate was 92.1% for paired and 80.9% for single sera from patients with typhus. The test gave results consistent with those given by the microimmunofluorescence (Micro-IF) and microagglutination (MA) tests. The sensitivity of the test was higher than Weil-Felix (P less than 0.05) test. This test was simple, rapid, sensitive and specific.     

斑疹伤寒五种血清学诊断方法的比较

本文报道微量免疫酶染色(Micro-IP)、微量免疫荧光(Micro-IF)、微量凝集试验(MA)、间接血凝试验(IHA)及外-斐氏反应(WF)5种方法检测斑疹伤寒抗体的比较。前4种方法的敏感性相近,均高于WF(P<0.025)。早期诊断以Micro-IP、Micro-IF检测IgM抗体为优,血清学分型宜用MA,血清流行病学调查此3种方法均适用,而IHA、WF不具备以上实用价值。     

微量凝集试验用于斑疹伤寒血清学诊断及流行病学调查的研究

<正> 目前,斑疹伤寒的诊断、分型以及流行病学调查的血清学方法均普遍应用补体结合(CF)试验。然而,它在诊断上阳性率较低,分型又受到原免疫状态的影响,且操作复杂。Fiset等应用立克次体感染鸡胚卵黄囊抗原建立了立克次体微量凝集(MA)技