新生儿脐血维生素D水平与IgE水平的相关性研究

目的 了解新生儿脐血维生素D水平及其与IgE水平的相关性。方法 测定185名新生儿脐血25-羟维生素D[25(OH)D]及总IgE值;采用问卷调查形式收集孕妇的健康状况及新生儿出生史;跟踪随访新生儿一年,了解其在随访期限内过敏性疾病发作情况。结果 新生儿脐血25(OH)D平均浓度为(49.63±16.56)nmol/L,中位数49.50nmol/L;脐血总IgE平均水平为(513.85±116.54)U/L;随访期限内共有38名婴儿有过敏状况发生;过敏组新生儿脐血25(OH)D水平(38.88±12.33)nmol/L,非过敏组新生儿脐血25(OH)D水平(52.41±16.41)nmol/L,两组对比差异有统计学意义(P<0.05);过敏组新生儿脐血IgE水平为(574.13±109.86)U/L,非过敏组新生儿脐血IgE水平(498.27±113.44)U/L,两组对比差异有统计学意义(P<0.05);采用多因素方差分析表明,家族过敏史与出生时脐血25(OH)D水平对脐血IgE水平产生影响(P<0.05);回归分析表明脐血25(OH)D水平与脐血IgE水平呈线性负相关(r²=0.576,P<0.05)。结论 脐血维生素D水平与IgE水平呈负相关,脐血维生素D水平的降低可能是生后一年内婴儿过敏症发生的危险因素。     

基因重组酵母测评系统在筛选环境样品雌激素活性中的应用研究

目的 用重组酵母测评系统研究DDTs和汽车尾气提取物的拟雌激素活性.方法 将终浓度为10~(-9)～10~(-15) mol/L的171β-雌二醇、终浓度为10~(-4)～10~(-9) mol/L 的DDTs(p,p'-DDT、o,p'-DDT、p,p'-DDD、p,p'-DDE)和终浓度为0.25～8.00ml/ml的汽车尾气提取物加入酵母细胞培养液,作用4 h,通过定量测定β-半乳糖苷酶活力表达受试物的雌激素作用强度.结果 DDTs是一类可直接象配体一样与雌激素受体(ER)结合并发挥雌激素效应的环境雌激素,单独作用时o,p'-DDT雌激素活性较高,p,p'-DDE的雌激索活性较低;汽油燃料车尾气在终浓度为2.00～4.00 ml/ml时,其β-半乳糖苷酶活力与DMSO对照组相比,差异有统计学意义(P＜0.05),天然气尾气各剂量组的β-半乳糖苷酶活力与对照组(蒸馏水)相比,差异无统计学意义(P＞0.05).结论 DDTs是经ER介导的环境雌激素,其中o,p'-DDT的拟雌激素活性最高.汽车尾气提取物中汽油燃料车尾气具有弱雌激素活性,天然气燃料车尾气未检出雌激素活性.

Abstract：

Objective To research the estrogen-like activity of the DDTs and the extract of vehicle exhaust by recombinant yeast system.Methods Being added into the yeast cell culture fluid,the objects reached the final concentration of 10~(-9)～10~(-15) mol/Lfor 17β-estradiol,10~(-4)～10~(-9) mol/L for DDTs(p,p'-DDT,o,p'-DDT,p,p'-DDD,p,p'-DDE),0.25～8.00 ml/ml for the extract of vehicle exhaust,then the cultivation was performed for 4 hours,the quantimtive assay of β-galactosidase activity accounts for the estrogenlike effect of the samples was carried out.Results DDTs were a group of environmental estrogens hormones,which could integrate estrogen receptors as the stimulators do and express the estrogen-like effect.Being tested separately.the DDTs showed the estrogen-like activity,which was stronger for o,p'-DDT and weaker for p,p'DDE.Theβ-galactosidase activity of gasoline-fueled vehicle exhaust at the final concentration of 2.00～4.00 ml/ml was significantly different from that of the DMSO(P＜0.05).while theβ-galactosidase activity of natural gas-fueled vehicle exhaust wasn't significantly difirerent from that of the contrast distilled water(P＜0.05).Conclusion DDTs are environmental estrogens mediated by ER,among which the strongest i8 o,p,-DDT.Gasoline-fueled vehicle exhaust shows weaker estrogen activity,natural gas-fueled vehicle exhaust shows no estrogen activity.     

选择性头部亚低温对34~35周缺氧缺血性脑病患儿的有效性及安全性研究

目的：回顾性比较亚低温对34~35周缺氧缺血性脑病（hypoxic?ischemic encephalopathy,HIE）患儿的有效性及安全性。方法：本中心既往3年来31例行选择性头部亚低温治疗的HIE患儿为亚低温组,30例同胎龄HIE患儿为对照组,所有患儿入院后收集临床资料（出生体重、胎龄、入院日龄、性别、Apgar评分、羊水情况,亚低温开始前及亚低温时心率、呼吸、氧饱和度、血压及并发症等情况）及外周血样白细胞数、中性粒细胞数、血糖、血气等检测,并行随访。结果：①亚低温组与对照组间在出生体重、胎龄等一般资料以及亚低温开始前心率、呼吸等基线资料均无明显差异;②入组时两组在辅助检查方面无显著差异;亚低温结束时两组间除神经元烯醇化酶（neuron specific enolase,NSE）外也无明显差异;出院前两组头颅MRI检查均示异常,但新生儿神经行为评定（neonatal behavioral neurological assessment,NBNA）评分亚低温组要好于对照组;③两组间除喂养不耐受外在其他并发症方面无显著差别;④亚低温组婴幼儿智能发育量表评分（children’s developmental center of China,CDCC）持续改善、好于对照组;头颅MRI在3、6个月随访时改善率好于对照组。对于重症HIE患儿,最终脑瘫发生率两组间无明显差异。结论：本研究提示选择性头部亚低温对于34~35周患儿有较高的脑保护价值和较好的安全性,但尚需进一步多中心的研究。