致死剂量照射的大鼠骨髓移植中输血和环磷酰胺对疗效的影响

本文以γ射线致死剂量照射大鼠研究了骨髓移植(BMT)中输血和环磷酰胺(CY)对疗效的影响.照射后60天各实验组的结果如下:仅用BMT组的活存率为55%,BMT前5天和后1天输血的两组活存率分别为0%和30%.BMT前1天输血组活存率提高到90%,而再用CY时则明显降低疗效.活存>10天和100天者均显示供体型细胞.结果表明,输血提高BMT对致死剂量照射的疗效与输血时间有关,而加用CY则降低疗效.     

重度放烧复合伤大鼠创面处理的实验研究

本文用不同方法对重度放烧复台伤大鼠创面进行了处理。结果显示, 伤后24小时植自体皮、异体皮或伍加输血时, 30天活存率和胸腺及脾细胞功能的恢复程度, 均显著高于对照组, 也高于单给Zn(SD)₂保痴或AgSD及zn(sD)₂保痂植皮组。但伤后7天植皮的动物25天内全部死亡。伤后1天, 痂下和内脏均未捡出细菌, 3～15天, 对照组内脏检出率随痴下增加而升高, 但24小时植皮组仅创面检出2例。这说明伤后24小时切痂植皮时效果最好, 主要因及早消除创面, 使感染减轻、免疫功能改善而有利于提高疗效。但极期应严禁切痂植皮。     

输不同处理血液对急性放射病治疗作用的实验研究

骨髓移植(ＢＭＴ)治疗急性放射病已有较多的经验和进展[1],但对急性放射病ＢＭＴ中多种输血方法与效果的比较研究较少。本研究自1983年开始,进行了54批实验,共用Ｗｉｓｔａｒ雌性受体大鼠1562只(雄性作供体)。在观察6～12Ｇｙ共7种照射剂量所致急性放射病的主要病程特点的基础上,研究了ＢＭＴ对不同剂量所致急性放射病的治疗效果,并着重从极重度骨髓型急性放射病异体ＢＭＴ方面采用多批次、多组合的输血方案进行对比研究,取得了较好的实验效果。一、对不同剂量全身照射的放射病ＢＭＴ作用特点的研究1不同受照剂量时早期造血细胞植入率的变化…     

放烧复合伤异体植皮后创面与皮肤病理学变化

目的观察放烧复合伤植皮后创面愈合与皮肤组织学的关系。方法采用5GY全身照射复合15％三度烧伤的放烧复合伤大鼠模型,伤后24h进行异体植皮封闭创面,术后1、3、5、7、10、20、25、30d分别取材。结果不治疗组动物10d时,创面较干燥,但伤后10～15d(溶痂期)死亡鼠创面有大部分破溃。放烧复合伤经输血和植皮的治疗组动物伤后7～10d时移植皮下仅有点状出血,此后逐渐被吸收,异体皮存活良好,20d开始长出新毛,30d已全部长毛。结论伤后24h切痂植皮,有利于保护创面、减轻感染。而伤后输注低淋巴细胞的照射血和异体植皮,使创面愈合延长异体皮活存。     

CHANGES OF IMMUNE FUNCTIONS AFTER RADIATION, BURNS AND COMBINED RADIATION-BURN INJURY IN RATS

The changes ot several immune functions were observed in rats after they were inflicted with 6 Gy gamma rays irradiation, 15% TBSA full thickness hrun and the combination of the 2 injuries. It was found that the ftmcldons of thymocytes and epienocytes suffered the most severe suppressinn in the 24th to 72nd hour after radiation injury and began to recover on the 7th day. In the rats with burn injury, the suppression on thymocytes and splenocytes were significantly less severe than that after radiation and recovered more rapidly. The effects of combined rediation-bura injury showed several characteristics. The suppression on the thymocytes was more severe with slower recovery as compared with that after single radiation injury only. The suppression on the splenocytes as a whole was similar to that after single radiation injury, but in the early stage after combined injury, the suppression was far more severe than that after radiation. Escharectccny and skin grafting on the burn wounds on the lst day after combined injury could accelerate the recovery on both the thymocytes and eplenocytes. Our findings indicated that the severity of the suppression on the immune functions due to combined radiadon-burrt injury might depend on the size of the burn wounds.     

Effects of blood transfusion on bone marrow transplantation for the survival of rats after total lymphoid irradiation treated

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模拟激波管的研制和应用

我室在有关部门协作下,研制了模拟激波管,经数年使用,效果良好。虽目前国内已建立更先进的生物用激波管(BCT-1型),但本模拟激波管(以下简称本管)仍有其实用价值,特简介如下: 1 设计原理 本管分为3段(见附图):(1)高压段可装TNT100g～200g。用铜质电雷管引爆。(2)实验段管     

烧伤、冲击伤及烧冲复合伤的实验观察

为了探索烧冲复合伤的一般规律,我们在实验室用狗分別造成烧伤,冲击伤和烧冲复合伤,并进行观察对比,现将初步结果报告于后。 实验方法 选成年健康杂种狗29只,体重9.9～14.0公斤。分成烧伤、冲击伤和烧冲复合伤三组。 烧伤组:致伤前2天将背部毛剪短,用20～30％硫化钠溶液脱毛,待皮肤干燥,用2％龙胆紫酒精溶液标出相当体表15％的面积。用5千瓦溴钨灯为光辐射烧伤光源,造成三度烧     

放射损伤复合烧伤对异体皮存活及某些免疫变化的影响

本文作者探讨了１５％Ⅲ度烧伤复合３～８Ｇｙ全身照射对大鼠异体皮存活及其免疫反应的影响。单烧组异体皮１０天全部排斥，早期免疫反应增强；而放烧复合伤时免疫反应明显受抑制，所植异体皮在复合３Ｇｙ或４Ｇｙ照射时，１０天存活率分别为２０％和３０％；复合５，６，８Ｇｙ者则上升为６９％，８８％和１００％，存活至３０天仍达３６％，４２％和１００％，与单烧植皮组相比，复合５Ｇｙ以上照射者均有显著性差异。表明放烧复合伤时异体皮存活率主要随受照射剂量增加而增高，随时间后延有所降低。