Excellent reliability of toe strength measurements in children aged ten to twelve years achieved with a novel fixed dynamometer

BackgroundStronger toe flexor muscles improve performance outcomes in children, including balance, sprinting, jumping and side stepping. Toe flexor strength (TFS) is recommended as part of the clinical assessment of foot function in children. Fixed dynamometry, rather than handheld, is the gold standard of measurement; however, it can be prohibitively costly. No fixed dynamometer reliability studies on toe flexion have been conducted in children to date.Research questionsDoes the novel fixed hand-held dynamometer (HHD) protocol provide reliable intra-rater and test-retest measurements of toe flexor strength in children aged 10 to 12?MethodsTwo trials were recorded from 14 healthy children (10–12 years), 7–14 days apart by the same rater. A Lafayette HHD (model 01163) measured peak force. The HHD was secured in a mobile custom mould below a step with a strap, which secured the foot of the participant. The receptor pads of the HHD were level with the upper surface of the step, maintaining neutral toe joints at rest. The participant was seated on an adjustable stool to ensure the hip, knee and ankle were each at 90° flexion, with the testing foot flat on the upper surface of the step. The averages of three maximal five second efforts were used for data analysis using a two-way mixed effects model with repeated measures ANOVA (intraclass correlation coefficient ICC 3,3). Standard error of measurement (SEM) was calculated to determine the absolute between trial variability.ResultsThe novel fixed HHD protocol provided excellent test-retest reliability with small measurement error for hallux (ICC 3,3 = 0.93, 95 % CI 0.78−0.98, SEM = 4.31 N) and lesser toe flexor strength testing (ICC 3,3 = 0.96, 95 % CI 0.87−0.99, SEM = 1.86 N).SignificanceThe fixed HHD protocol described in this study has excellent reliability for the test-retest evaluation of children’s toe flexor strength.     

Construction of a protocol for measuring blood flow by two-dimensional phase-contrast MRA

Our aim is to describe and demonstrate the steps we have found to be useful in the construction and evaluation of protocols for triggered and nontriggered measurement of blood flow by two-dimensional phase-contrast magnetic resonance angiography (MRA). To achieve this goal, we start with a survey of factors governing the accuracy (validity) and precision (repeatability) of MR flow measurements. This knowledge, combined with prior information regarding the diameter of the target vessel and the prevailing flow conditions, is then employed to define a protocol for measuring flow with negligible systematic error. In the absence of a gold standard for in vivo flow measurements, the protocol is subsequently validated for a range of flow conditions by representative phantom experiments. Precision is then calculated from the signal-to-noise ratio (SNR) of blood in the accompanying magnitude images or, less conveniently, estimated from the standard deviation of repeated measurements. The desired precision is finally achieved by adjusting the appropriate SNR parameters. All steps involved in protocol development are demonstrated for both flow-independent and flow-dependent acquisitions.     

Complex DNA mixture analysis in a forensic context: Evaluating the probative value using a likelihood ratio model

The interpretation of mixed DNA profiles obtained from low template DNA samples has proven to be a particularly difficult task in forensic casework. Newly developed likelihood ratio (LR) models that account for PCR-related stochastic effects, such as allelic drop-out, drop-in and stutters, have enabled the analysis of complex cases that would otherwise have been reported as inconclusive. In such samples, there are uncertainties about the number of contributors, and the correct sets of propositions to consider. Using experimental samples, where the genotypes of the donors are known, we evaluated the feasibility and the relevance of the interpretation of high order mixtures, of three, four and five donors.The relative risks of analyzing high order mixtures of three, four, and five donors, were established by comparison of a ‘gold standard’ LR, to the LR that would be obtained in casework. The ‘gold standard’ LR is the ideal LR: since the genotypes and number of contributors are known, it follows that the parameters needed to compute the LR can be determined per contributor. The ‘casework LR’ was calculated as used in standard practice, where unknown donors are assumed; the parameters were estimated from the available data. Both LRs were calculated using the basic standard model, also termed the drop-out/drop-in model, implemented in the LRmix module of the R package Forensim.We show how our results furthered the understanding of the relevance of analyzing high order mixtures in a forensic context. Limitations are highlighted, and it is illustrated how our study serves as a guide to implement likelihood ratio interpretation of complex DNA profiles in forensic casework.     

Diffuse interstitial lung disease: overlaps and uncertainties

Histopathological analysis of lung biopsy material allows the diagnosis of idiopathic interstitial pneumonias; however, the strength of this diagnosis is sometimes subverted by interobserver variation and sampling. The American Thoracic Society and European Respiratory Society recommendations of 2002 provide a framework for the diagnosis of interstitial lung disease (ILD) and proposed an integrated clinical, radiological and histopathological approach. These recommendations represent a break with tradition by replacing the ‘gold standard’ of histopathology with the combined ‘silver standards’ of clinical, imaging and histopathological information. One of the pitfalls of a rigid classification system for the diagnosis of interstitial lung disease is its failure to accommodate the phenomenon of overlapping disease patterns. This article reviews the various ways that interstitial lung disease may be classified and discusses their applicability. In addition the issue of overlap disease patterns is considered in the context of histopathological interobserver variation and sampling error and how a pigeonhole approach to disease classification may overlook these hybrid entities.     

Femoral neck torsion angle measurement by computed tomography

To establish the accuracy and reproducibility of measuring femoral neck torsion angles (FNTA) on CT images, this angle was measured on 12 dry femora directly on the gross specimens, on plain radiographs, and on CT images. The selection of the CT level, both at the femoral neck and the condyles, that may affect the measurement of FNTA was analyzed. The CT measurements of FNTA were found to have a reproducibility of 2.5 and 0 degrees mean offset error when compared with readings on plain radiographs. A new CT method was developed to measure this angle particularly in patients with coxa valga in whom this measurement is difficult if not impossible to accomplish by current methods. For this purpose composite CT images were made of the femoral head and the femoral shaft, immediately below the lesser trochanter. On these images the line connecting the centers of the femoral head and shaft were inferred to represent the cervical plane. In comparison with the standard CT method, this new method had the same reproducibility, although it slightly overestimated this angle by 2 degrees. This degree of error is negligible when compared with the normal variations of this angle, which ranges from -20 to +38 degrees. Furthermore, it compares favorably with the limited accuracy of derotational osteotomy surgery.     

核酸适体和胶体金检测皮质醇含量的方法与应用

目的通过指数富集配体系统进化（SELEX）技术获得皮质醇特异性核酸适体,应用核酸适体和胶体金建立一种灵敏的定量检测皮质醇含量的方法。方法采用柠檬酸钠还原法和硼氢化钠还原法制备胶体金,用核酸适体、胶体金聚集显色法检测皮质醇含量。结果相比20nm胶体金,采用5nm和13nm胶体金颗粒具有较好的灵敏度、重复性和线性范围,5nm胶体金颗粒用于皮质醇的检测的线性范围为10．1～320μmol／L,标准曲线相关系数r为0．9993,其重复性较好,标准差小于平均值10％。结论本实验建立了一种高灵敏性的能特异性地检测皮质醇标准品含量的方法。     

MRI of the cervical spine with neck extension: is it useful?

Objectives

Standard MRI of the cervical spine is performed in a different anatomical position to that utilised for traditional contrast myelography. Those well practised in myelography are familiar with the considerable changes in configuration of the bony and soft tissues of the cervical spine that may occur with changes in the degree of neck flexion and extension. We set out to compare the findings in a select group of patients with myeloradiculopathy who had undergone myelography and MRI in both standard and neck-extended positions. These findings were correlated with the clinical status.

Methods

29 patients underwent myelography with CT (CTM) and MRI in neutral and neck-extended positions. The imaging was assessed for the degree of cord compression and neural foraminal narrowing, quantified using a simple grading scheme suitable for routine clinical practice. The degree of neck extension was assessed using an angular measurement.

Results

For both CTM and MRI, scanning with the neck extended significantly increases the severity of cord compression compared with the standard supine position, to a degree similar to that shown during conventional prone myelography. The degree of perceived cord compression is related to the degree of neck extension achieved. Correlation of standard MRI findings and the clinical level of radiculopathy is poor. This correlation improves when the neck is extended.

Conclusions

The most appropriate position for routine MRI of the cervical spine in degenerative disease remains unknown, but in selected patients imaging with the neck extended may provide important additional information.With the introduction of spinal surface coil technology and gradient echo sequences in the late 1980s, MRI rapidly replaced intrathecal contrast myelography as the standard imaging method for assessment of the cervical spinal cord and nerve roots. The two investigations are performed in very different anatomical positions. Standard cervical spine MRI is performed in a coil that is designed to make the patient comfortable, to minimise movement-related artefacts. This generally results in a position of mild extension of the neck. On the other hand, the majority of images for plain cervical myelography are obtained with the patient prone and the neck hyperextended so as to retain myelographic contrast within the cervical lordosis. CT myelography (CTM) is typically performed with the patient supine and with the neck straight or mildly flexed; however, CT myelography can also be performed in the prone position with the neck extended [1].For a number of years we continued to use plain myelography, with CTM as a second-line investigation in patients with cervical myelopathy and/or radiculopathy, when the results of standard MRI were inconsistent with symptoms and signs. More recently we have also performed MRI with neck extension, predominantly in patients with myelopathic features but inconclusive supine MRI. We have been unsure how extended MRI compares with myelography and whether it is able to produce the same degree of extension as myelography. We have performed CT in the prone extended position as well, but have also been unsure how that relates to standard supine CT imaging or to extended MRI. We therefore wished to compare these various modalities in a heterogeneous group of clinically problematic patients.     

Measurement of scapula upward rotation: a reliable clinical procedure

Background: It is important to deal with the scapula when developing rehabilitation strategies for the shoulder complex. This requires clinical measurement tools that are readily available and easy to apply and which provide a reliable evaluation of scapula motion. Method: Twenty six patients were assessed in two repeat tests within a single testing session. Patients exhibiting a wide spectrum of shoulder pathology were selected. The angle of scapular upward rotation was measured during total shoulder abduction. The measurement protocol was performed twice during a single testing session by a single tester. Results of the two tests were compared and the reliability assessed by intraclass correlation coefficients (ICCs). Results: There was no significant difference in the scapula measurements taken during the two tests at each testing position. Overall, there was very good intrarater reliability (ICC = 0.88). The ICC ranged from 0.81 (at 135°) to 0.94 (at both resting and end of total shoulder abduction range). Conclusion: The Plurimeter-V gravity inclinometer can be used effectively and reliably for measuring upward rotation of the scapula in all ranges of shoulder abduction in the coronal plane.     

Consistent with: what doctors say and jurors hear

While there is an extensive body of literature regarding the factors that influence how juries interpret medical expert statements, there is limited empirical evidence regarding the effects of word choice. The term ‘consistent with’ is widely used in legal proceedings and expert witness statements, so it is important to understand what this term means to a jury. The aim of this research is to understand how potential jurors interpret the phrase ‘consistent with’ in a forensic medical context. Potential jurors were recruited for a survey containing three mock vignettes where the doctor stated that the injury was ‘consistent with’ either self-inflicted injury, accidental injury, or injury by a third party. Participants were asked what the doctor meant, using a range of options taken from the United Nations’ Istanbul Protocol definitions of medico-legal terms used to describe lesions caused by torture. The results from 151 surveys indicate there was no consistent interpretation of the phase within each vignette or between vignettes. This research demonstrates that the phrase ‘consistent with’ can be interpreted in a variety of ways: caution is therefore required when using this expression in legal proceedings. Further research regarding the best choice of terms to use in court is required.     

Evaluation of three methods for measurement of femoral neck anteversion. Femoral neck anteversion, definition, measuring methods and errors

A variety of methods exist for determining femoral neck anteversion. There is, however, no consistent definition or reference method by which the correctness of the measurements can be determined. In this report the precision of a specific definition of femoral neck anteversion is estimated by means of a reference method. Three different radiologic measuring methods based on fundamentally different principles are evaluated by comparison with this reference. It is concluded that: 1) The Dunlap/Rippstein method can be recommended as the standard method. 2) Computed tomography may have substantial sources of error. 3) Methods based on arbitrary projections have poor precision.     

Hypoxia: importance in tumor biology, noninvasive measurement by imaging, and value of its measurement in the management of cancer therapy

PURPOSE: The Cancer Imaging Program of the National Cancer Institute convened a workshop to assess the current status of hypoxia imaging, to assess what is known about the biology of hypoxia as it relates to cancer and cancer therapy, and to define clinical scenarios in which in vivo hypoxia imaging could prove valuable. RESULTS: Hypoxia, or low oxygenation, has emerged as an important factor in tumor biology and response to cancer treatment. It has been correlated with angiogenesis, tumor aggressiveness, local recurrence, and metastasis, and it appears to be a prognostic factor for several cancers, including those of the cervix, head and neck, prostate, pancreas, and brain. The relationship between tumor oxygenation and response to radiation therapy has been well established, but hypoxia also affects and is affected by some chemotherapeutic agents. Although hypoxia is an important aspect of tumor physiology and response to treatment, the lack of simple and efficient methods to measure and image oxygenation hampers further understanding and limits their prognostic usefulness. There is no gold standard for measuring hypoxia; Eppendorf measurement of pO(2) has been used, but this method is invasive. Recent studies have focused on molecular markers of hypoxia, such as hypoxia inducible factor 1 (HIF-1) and carbonic anhydrase isozyme IX (CA-IX), and on developing noninvasive imaging techniques. CONCLUSIONS: This workshop yielded recommendations on using hypoxia measurement to identify patients who would respond best to radiation therapy, which would improve treatment planning. This represents a narrow focus, as hypoxia measurement might also prove useful in drug development and in increasing our understanding of tumor biology.     

Different imaging techniques in the head and neck: Assets and drawbacks

In this review, the gold standard imaging techniques for the head and neck and the latest upcoming techniques are presented, by comparing computed tomography (CT), magnetic resonance imaging and positron emission tomography-CT, as well as ultrasound, depending on the examined area. The advantages and disadvantages of each examination protocol are presented. This article illustrates the connection between the imaging technique and the examined area. Therefore, the head and neck area is divided into different sections such as bony structures, nervous system, mucous membranes and squamous epithelium, glandular tissue, and lymphatic tissue and vessels. Finally, the latest techniques in the field of head and neck imaging such as multidetector CT, dual-energy CT, flash CT, magnetic resonance angiography, spectroscopy, and diffusion tensor tractography using 3 tesla magnetic resonance are discussed.     

Estimating and comparing diagnostic tests' accuracy when the gold standard is not binary

RATIONALE AND OBJECTIVES: Investigators often need to assess the accuracies of diagnostic tests when the gold standard is not binary-scale. The objective of this article is to describe nonparametric estimators of diagnostic test accuracy when the gold standard is continuous, ordinal, and nominal scale. MATERIALS AND METHODS: A nonparametric method of estimating and comparing the area under receiver operating characteristic (ROC) curves, proposed by DeLong et al, is extended to situations in which the gold standard is not binary. Two examples illustrate the methods. RESULTS: Measures of diagnostic test accuracy, their variance, and tests for comparing two diagnostic tests' accuracies in paired designs are presented for situations in which the gold standard is continuous, ordinal, and nominal scale. These summary measures of diagnostic test accuracy are analogous in form and interpretation to the area under the ROC curve. CONCLUSION: Dichotomizing the outcomes of a gold standard so that traditional ROC methods can be applied can lead to bias. The methods described here are useful for assessing and comparing summary test accuracy when the gold standard is not binary scale. They have limitations similar to other summary indices.     

Clinical assessment of freezing of gait in Parkinson's disease from computer-generated animation

The current ‘gold standard’ for clinical evaluation of freezing of gait (FOG) in Parkinson's disease (PD) is determination of the number of FOG episodes from video by independent raters. We have previously described a robust technique for objective FOG assessment from lower-limb acceleration. However, there is no existing method for validation of autonomous FOG measures in the absence of video documentation. In this study we compared the results of clinical evaluation of FOG from computer-generated animations (derived from body-mounted inertial sensors) during a timed up and go test with the ‘gold standard’ of clinical video assessment, utilizing a cohort of 10 experienced raters from four PD centers. Agreement between the 10 clinical observers for scoring of FOG from computer animations was more robust for the relative duration of freeze events (percent time frozen; intraclass correlation coefficient of 0.65) than number of FOG episodes, and was comparable with clinical evaluation of the patient from video (intraclass correlation coefficient 0.73). This result suggests that percent time frozen should be considered (along with number of FOG events) to better convey FOG severity. The ability of clinical observers to quantify FOG from computer-generated animation derived from lower-limb motion data provides a potential approach to validation of accelerometry-based FOG identification outside of the clinic.     

Assessment of maximal fat oxidation during exercise: A systematic review

Maximal fat oxidation during exercise (MFO) and the exercise intensity eliciting MFO (Fat_max) are considered biological markers of metabolic health and performance. A wide range of studies have been performed to increase our knowledge about their regulation by exercise and/or nutritional intervention. However, numerous data collection and analysis approaches have been applied, which may have affected the MFO and Fat_max estimation. We aimed to systematically review the available studies describing and/or comparing different data collection and analysis approach factors that could affect MFO and Fat_max estimation in healthy individuals and patients. Two independent researchers performed the search. We included all original studies in which MFO and/or Fat_max were estimated by indirect calorimetry through an incremental graded exercise protocol published from 2002 to 2019. This systematic review provides key information about the factors that could affect MFO and Fat_max estimation: ergometer type, metabolic cart used, warm‐up duration and intensity, stage duration and intensities imposed in the graded exercise protocol, time interval selected for data analysis, stoichiometric equation selected to estimate fat oxidation, data analysis approach, time of the day when the test was performed, fasting time/previous meal before the test, and testing days for MFO/Fat_max and maximal oxygen uptake assessment. We suggest that researchers measuring MFO and Fat_max should take into account these key methodological issues that can considerably affect the accuracy, validity, and reliability of the measurement. Likewise, when comparing different studies, it is important to check whether the above‐mentioned key methodological issues are similar in such studies to avoid ambiguous and unacceptable comparisons.     

Estimating human energy expenditure: a review of techniques with particular reference to doubly labelled water

This review includes an historical overview of the techniques for measuring energy expenditure (EE). Following this overview, the "gold standard" method of measuring EE, the doubly labelled water (DLW) method, is emphasised. Other methods, such as direct calorimetry, indirect calorimetry systems, heart rate and EE relationships, questionnaires and activity recall, motion sensors, combined heart rate and motion sensors for the estimation of EE are then highlighted in relation to their validation against the DLW method. The major advantages and disadvantages for each method are then considered. The preferred method to determine EE is likely to depend principally on factors such as the number of study participants to be monitored, the time period of measurements and the finances available. Small study participant numbers over a short period may be measured accurately by means of indirect calorimetric methods (stationary and portable systems). For periods over 3-4 days, EE should ideally be measured using the DLW method. However, the use of motion sensors is very promising in the measurement of EE, and has a number of advantages over the DLW method. Furthermore, if used correctly, both heart rate and questionnaire methods may provide valuable estimates of EE. Additional studies are needed to examine the possibility of improving the accuracy of measurement by combining two or more techniques. Such information, if confirmed by scientific rigour, may lead to an improvement in the estimation of EE and population-based physical activity levels. The accurate measurement of physical activity and EE is critical from both a research and health prospective. A consideration of the relevant techniques used for the estimation of EE may also help improve the quality of these frequently reported measurements.     

Normalized BMD as a predictor of bone strength

RATIONALE AND OBJECTIVES: In the noninvasive evaluation of bone quality, bone mineral density (BMD) has been shown to be the single most important predictor of bone strength and osteoporosis-related fracture. Among the methods of measuring BMD, dual x-ray absorptiometry (DXA) has widespread acceptance due to its low radiation, low cost, and high precision. However, DXA measures area BMD instead of true volumetric density; thus, a larger bone will tend to have a high BMD than will a smaller bone. Therefore, the comparison of BMDs of bones of different sizes can be misleading. In this study, the authors tried to compensate for the size effect by normalizing the area BMD with bone size as measured from a standard pelvic radiograph. MATERIALS AND METHODS: The overall method for calculation of normalized BMD included conventional area-based BMD from DXA and the extraction of geometric measures from pelvic radiographs. The database for analysis included 34 femoral neck specimens. Regression analysis was performed between the normalized volumetric BMD, measured from femoral neck region, and the mechanical properties obtained from trabecular bone cubes machined from the same region. RESULTS: After normalization of the area BMD, the coefficient of determination increased from 0.30 to 0.43 for the Young modulus and from 0.27 to 0.37 for bone compressive strength. CONCLUSION: A noninvasive method of normalizing BMD can improve the prediction of bone mechanical properties and has potential in monitoring changes in growing skeletons and in the clinical evaluation of bone quality.     

Implementation and Results of a Percutaneous Renal Allograft Biopsy Protocol to Reduce Complication Rate

Percutaneous renal transplant biopsy (PRTB) is the gold standard for evaluating allograft rejection after renal transplant. Hemorrhage is the predominant complication. We describe the implementation of a standardized protocol for PRTB at a single institution, with the aim of reducing bleeding complications. Utilizing the plan-do-study-act model for quality improvement, we created and deployed a protocol centered on controlling patient’s hypertension, platelet function, and anticoagulation status. The 4-year study encompassed a total of 880 PRTBs, before and after implementation of the protocol. Total complication rate, which was 5.8% in the 2 years leading up to implementation of the protocol, was reduced to 2.9% after the protocol was introduced (P = .04). A standardized approach to PRTB can potentially lower complication rates; we present a framework for implementating a quality improvement protocol at other institutions.     

Identification of factors associated with diagnostic performance variation in reporting of mammograms: A review

ObjectivesThis narrative review aims to identify what factors are linked to diagnostic performance variation for those who interpret mammograms. Identification of influential factors has potential to contribute to the optimisation of breast cancer diagnosis. PubMed, ScienceDirect and Google Scholar databases were searched using the following terms: ‘Radiology’, ‘Radiologist’, ‘Radiographer’, ‘Radiography’, ‘Mammography’, ‘Interpret’, ‘read’, ‘observe’ ‘report’, ‘screen’, ‘image’, ‘performance’ and ‘characteristics.’ Exclusion criteria included articles published prior to 2000 as digital mammography was introduced at this time. Non-English articles language were also excluded. 38 of 2542 studies identified were analysed.Key findingsInfluencing factors included, new technology, volume of reads, experience and training, availability of prior images, social networking, fatigue and time-of-day of interpretation. Advancements in breast imaging such as digital breast tomosynthesis and volume of mammograms are primary factors that affect performance as well as tiredness, time-of-day when images are interpreted, stages of training and years of experience. Recent studies emphasised the importance of social networking and knowledge sharing if breast cancer diagnosis is to be optimised.ConclusionIt was demonstrated that data on radiologist performance variability is widely available but there is a paucity of data on radiographers who interpret mammographic images.Implications for practiceThis scarcity of research needs to be addressed in order to optimise radiography-led reporting and set baseline values for diagnostic efficacy.