化疗栓塞后肝细胞癌细胞凋亡和耐药基因表达的改变

目的：研究肝细胞癌患者经动脉化疗栓塞（ＴＡＣＥ）治疗后肿瘤的细胞凋亡与耐药基因表达的情况及两者的关系。方法：经病理证实的肝细胞癌９８例,单纯手术５７例,４种介入治疗后Ⅱ期手术切除４１例,用流式细胞集资检测细胞凋亡,用链霉亲合素－生物素酶复合免疫组化检测各标本中Ｐｇｐ。结果：肿瘤细胞凋亡率和Ｐｇｐ阳性百分数在单纯手术组和介入Ａ、Ｂ、Ｃ、Ｄ各组分别为１．７７％、６．１％、８．７％、１３．３％、１５．７     

化疗栓塞对肝细胞癌转移抑制基因nm23/ndpk表达的影响--介入治疗与肿瘤转移潜能研究之一

目的 研究转移抑制基因ｎｍ－２３／ｎｄｐｋ在原发性肝细胞癌（ＰＨＣ）中表达的意义以及不同化疗栓塞方法对其表达的影响。材料与方法 ７种不同组织学类型的ＰＨＣ共９８例,单纯手术５７例,４种介入治疗后Ⅱ期切除４１例。采用链霉亲合素生物素酶复合物（ＳＡＢＣ）免疫组化方法,检测各样本中ｎｍ－２３／ｎｄｐｋ的表达,并与临床转移情况对照,结果 ｎｄｐｋ阳阴性者转移率分别为４１．２％、８０．０％（Ｘ＾２＝１２．６     

肝细胞癌化疗栓塞方法和肿瘤坏死

目的 评价地细胞癌肿瘤坏死与经动脉化疗栓塞（ＴＡＣＥ０方法的关系。材料与方法 观察１１７例手术观察的肝细胞癌肿块坏死和增长率,其中单纯手术５８例,４种ＴＡＣＥ后Ⅱ期手术切除５９例。结果 肝细胞癌ＴＡＣＥ后环死较自发坏死严重、范围广（Ｐ〈０．０１）,坏死程度和愉体积变化与ＴＡＣＥ方法密切相关（多全塞较单材料栓塞、单纯化疗改变明显,Ｐ〈０．０１）,但与ＴＡＣＥ和手术切除间期无显著相关（Ｐ〉０．０５）,     

介入治疗对肝细胞癌耐药基因表达的影响

目的 研究不同介入方法对不同组织学类型的原发性肝细胞癌（Ｐｒｉｍａｒｙｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍａ,ＰＨＣ）多药耐药（ｍｕｔｉｄｒｕｇｒｅｓｉｓｔａｎｃｅＭＤＲ）基因表达蛋白（Ｐ－ｇｌｙｃｏｐｒｏｔｅｉｎ,Ｐｇｐ）的影响。方法 ７种不同组织学类型的ＰＨＣ共９８例,单纯手术５７例,４种介入方法治疗后Ⅱ期切除４１例,采用链霉亲合素－生物素酶复合物（ｓｔｒｅｐｔａｖｉｄｉｎ－ｂｉｏｔｉ     

术前介入治疗对非小细胞性肺癌细胞凋亡的影响

目的：研究介入治疗对非小细胞肺癌细胞凋亡的影响，探讨介入治疗术前应用的疗效。材料和方法：采用流式细胞仪与免疫组化方法检查介入治疗后行手术35例标本和单纯手术31例标本的细胞凋亡率、p53和bcl-2蛋白表达。结果：介入治疗组的细胞凋亡率明显高于单纯手术组（P＜0．05）；介入治疗组的p53蛋白阳性表达明显低于单纯手术组（P＜0．05）；介入治疗组的bcl-2蛋白阳性表达与单纯手术组相比略有升高，但无显著性差异（P＞0．05）。结论：介入治疗可促进细胞凋亡，近期疗效较好，同时术前介入治疗可诱导肿瘤细胞的多药耐药性产生，疗程不宜过长。     

肝动脉栓塞化疗联合B超引导下无水乙醇注射治疗25例肝癌疗效观察

目的：探讨肝动脉栓塞化疗术（TACE）联合B超引导下瘤内注射无水乙醇（PEI）治疗原发性肝细胞癌的疗效。方法：对不能手术切除的原发性肝细胞癌，采用TACE联合PEI，同单纯TACE效果进行比较。结课：经过治疗后，TACE组15例肿瘤体积缩小，缩小率为62．5％（15／24）；TACE＋PEI组22例肿瘤体积缩小，缩小率88％（22／25）；TACE4-PEI组治疗后1年、2年、3年的生存率显著高于单纯TACE组（P〈O．05）。结论：TACE4-PEI治疗原发性肝细胞癌的疗效显著优于单纯TACE。     

误诊为肝细胞癌的病例分析

目的；提高肝细胞癌（ＨＣＣ）的诊断正确率。材料与方法：回顾性分析外科治疗前５例误诊为ＨＣＣ的临床、影像学和实验室检查以及正确诊断、治疗和随访结果等。结果：５例病理这证实分别为肝细胞腺这囊腺瘤恶性变、孤立性肝转移腺癌、腹膜恶性纤维组织细胞和外生型胃神经鞘瘤。５例中３便因瘤体巨大富血而未能作手术切除，４例术曾接受介入治疗或化疗。３例良性（包括伴有恶性变１例）３年后仍存活。结论：术前发能辨认肝细胞瘤和胆     

TAE治疗原发肝细胞癌(对不同组织细胞学类型疗效的初步研究)

目的：通过对一组１５７例经组织细胞学检查证实的原发性肝细胞癌患者生存资料及影像学资料的分析，初步研究经导管动脉栓塞（ＴＡＥ）对不同组织细胞学类型肝细胞癌的治疗效果。材料与方法：８种不同组织细胞学类型的资料较完整的原发性肝细胞癌患者，共计１５７例，分别接受单纯ＴＡＥ、单纯手术或ＴＡＥ＋手术切除治疗。对上述病例分别按照不同组织细胞学类型及不同治疗方法进行生存资料和影像学资料分析。结果：本组原发性肝细胞癌的１年、２年、３年和５年的累计生存率分别为７４．５２％、４８．４０％、３２．４８％和１１．４６％。各组织细胞学类型中，纤维板层型与透明细胞型肝细胞癌的５年生存率分别为２５％和２９％，。三种治疗方法中，单纯ＴＡＥ组、单纯手术组与ＴＡＥ＋手术组的平均生存时间分别为２５．０６（标准误＝１．８７）、３０．３８（标准误＝２．０５）和７２．７２（标准误＝６．９０）个月。结论：ＴＡＥ对不同组织细胞学类型的原发性肝细胞癌在治疗效果上存在差异；本组中，以透明细胞型肝细胞癌对ＴＡＥ较为敏感，而以小细胞型和低（未）分化型肝细胞癌较不敏感。     

特殊类型肝细胞癌的病理学与影像学表现

特殊类型肝细胞癌的病理学与影像学表现王艳萍张金山高育随着肝细胞癌（ｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａ，ＨＣＣ）介入治疗学方面的不断进展，对特殊类型ＨＣＣ的诊断与鉴别诊断正在成为制订临床治疗方案的基础［１］。根据日本肝癌研究会（ＬｉｖｅｒＣ...     

肝巨大占位性病变肝三叶切除23例治疗分析

目的：探讨肝巨大占位性病变的外科治疗,提高肝三叶切除治疗水平。方法：回顾分析肝三叶切除治疗肝巨大占位性病变２３例（肝肿瘤５例,肝包虫病１８例）治疗过程。结果：手术死亡率（１／２３）４．３５％,术后并发症（４／２３）１７．４％,临床治愈率（２２／２３）９５．６５％。临床治愈出院２２例,随访１、３、５年的生存率分别为９０．９５％、８６．４％和８１．８％。肝癌与肝包虫病的临床治愈率相当,５年生存率前者明显低于后者,与癌复发有关。结论：严格手术指征,围手术期处理,注重手术操作技巧,可提高临床治愈率和术后疗效。     

The role of interventional radiology in trauma

Introduction Interventional Radiology has evolved into a specialty having enormous input into the care of the traumatized patient.In all hospitals,regardless of size,the Interventional Radiologist must consider their relationships with the trauma service in order to     

Carbohydrate Supplementation during Exercise: Does It Help? How Much is Too Much?

KEY POINTS· Carbohydrate intake during exercise can delay the onset of fatigue and improve performance of prolonged exercise as well as exercise of shorter duration and greater intensity (e.g., continuous exercise lasting about 1h and intermittent high-intensity exercise), but the mechanisms by which performance is improved are different.     

Diagnóstico ecográfico del neumotórax

The ultrasonographic diagnosis of pneumothorax is based on the analysis of artifacts. It is possible to confirm or rule out pneumothorax by combining the following signs: lung sliding, the A and B lines, and the lung point. One fundamental advantage of lung ultrasonography is its easy access in any critical situation, especially in patients in the intensive care unit. For this reason, chest ultrasonography can be used as an alternative to plain-film X-rays and computed tomography in critical patients and in patients with normal plain films in whom pneumothorax is strongly suspected, as well as to evaluate the extent of the pneumothorax and monitor its evolution.     

磁共振DWI和动态增强鉴别颅底脊索瘤和侵袭性垂体瘤的应用

目的 探讨磁共振扩散加权成像(DWI)和动态增强在颅底脊索瘤和侵袭性垂体瘤(IPA)鉴别诊断中的应用价值.方法 搜集经手术病理证实且影像学有鞍区破坏的颅底脊索瘤患者15例、向鼻咽部侵犯的IPA患者20例.测量二者的表观扩散系数(ADC)值,绘制受试者工作特征曲线(ROC),分析动态增强曲线的类型,统计达峰时间(TTP)、增强峰值(EP)和最大对比增强率(MCER),分析各个参数在鉴别诊断中的价值.结果 颅底脊索瘤的ADC值为(1.274±0.07)×10-3mm2/s,高于IPA ADC值(0.672±0.03) ×10-3 mm2/s(P ＜0.001),ADC阈值为0.964×10-3mm2/s时,ROC曲线下面积为0.997,敏感度为93.3％,特异度为100％.颅底脊索瘤时间-信号强度曲线(TIC)Ⅰ型14例,TICⅢ型1例,此例TICⅢ型者TTP约40 s;IPA TIC Ⅰ型7例,TICⅢ型13例.颅底脊索瘤和IPA的EP、MCER差异均有统计学意义(P ＜0.001).结论 ADC值和TIC的类型及其相关参数(EP,MCER)有助于颅底脊索瘤和IPA之间的鉴别.     

Treatment of intrahepatic arteriovenous fistula in hepatocellular carcinoma with N-butyl-2-cyanoacrylate

Primary hepatocellular carcinoma (HCC) continues to be one of the most common malignancies with an incidence of approximately one million cases per year and a dismal prognosis; some authors have reported a median survival of 1 ～ 2 months after diagnosis. Although surgery remains the only hope for cure, few patients are candidates[1,2].     

U50,488H对正常及缺氧心肌细胞L型钙电流的作用

目的 探讨心脏阿片受体和β-受体相互作用的机制。方法 采用全细胞膜片钳技术，观察U50，488H(β-阿片受体选择性激动剂)对正常和缺氧心肌细胞L型钙电流的作用。结果 U50，488H剂量依赖性(0．1～100μmol／L)抑制正常心肌细胞的L型钙电流及异丙肾上腺素(0．1μmol／L)激动的钙电流，而细胞缺氧后，这一抑制作用减弱；U50，488H对Forskolin(10μmol／L)激动的L型钙电流无明显影响。结论 β-阿片受体对β-受体信号的负性调节作用在细胞缺氧后减弱，其作用位点可能发生于β-受体与腺苷酸环化酶环节之间。     

Overtraining syndrome : la piste de la suractivité ?

Introduction

Overtraining is clearly a multifactorial disturbance. We aimed at evaluating the relative importance of overactivity and nutritional imbalances.

Methods

A comparison of 17 footballers with seven subjects with a diagnosis of overtraining evidenced that the two groups differ by energy expenditure resulting from physical activity (1573 ± 378.6 kcal/d from 804.7 ± 184.7 kcal/d) while food intake does not exhibit significant differences.

Conclusion

In this sample of subjects, inadequate food intake is found in both groups, so that overtraining appears rather characterized by a higher level of physical activity. Therefore, a relative energy deficit resulting from overactivity rather than dietary mistakes appears to explain the syndrome in these athletes, who are thus truly “overtrained”.     

Prenatal detection of brain anomalies with ultrasonography

Ultrasound is the first-line imaging study of the fetal brain performed at 12, 22 and 32 weeks of gestation. The very structured evaluation of biometric and morphological data, facilitated by the use of a checklist, ensures a high quality examination. All patients where an anomaly of any key element is suspected should be referred to a center dedicated to prenatal diagnosis for confirmation irrespective of the gestational age. Prenatal diagnosis plays a significant role in France since medical interruption of pregnancy until term is authorized.