黄海葵附生真菌Penicillium thomii的化学成分

目的研究黄海葵(anemone)附生真菌Penicillium thomii的化学成分.方法用各种色谱技术进行分离纯化,根据理化性质和波谱数据进行结构鉴定.结果从黄海葵附生真菌Penicillium thomii菌丝体中分离得到5个化合物,分别为青霉酮A(I),对甲基苯甲酸(II),1-O-十六碳烷酰-2-O-(9-十八碳烯酰)-3-O-(9,12-十八碳二烯酰)甘油酯(III),5α,8α-环二氧-24ζ-甲基胆甾-6,22-二烯-3β-醇(IV)和大黄素(V).结论青霉酮A(I)为新化合物,化合物II,III,IV和V系首次从该属中分离得到.     

黄海葵附生真菌Penicillium thomii的化学成分

目的研究黄海葵(anemone)附生真菌Penicillium thomii的化学成分。方法用各种色谱技术进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果从黄海葵附生真菌Penicillium thomii菌丝体中分离得到5个化合物,分别为青霉酮A(I),对甲基苯甲酸(II),1-O-十六碳烷酰-2-O-(9-十八碳烯酰)-3-O-(9,12-十八碳二烯酰)甘油酯(III),5α,8α-环二氧-24ζ-甲基胆甾-6,22-二烯-3β-醇(IV)和大黄素(V)。结论青霉酮A(I)为新化合物,化合物II,III,IV和V系首次从该属中分离得到。     

褐藻铁钉菜化学成分的研究(Ⅱ)

采用稻瘟霉模型生物活性追踪方法，应用多种层析手段，从采自浙江南麂岛的褐藻铁钉菜(Ishigeokamurai)中分离得到6个化合物，应用多种波谱技术，分别确定为：3β，28ξ-二羟基-24-乙基-5，23Z-胆甾二烯(J)，13^2S-羟基脱铁叶绿素a(Ⅱ)。脱镁叶绿素a(Ⅲ)，1-O-二十六烷酰基甘油酯(Ⅳ)，1-O-十六烷酰基甘油酯(V)和l-O-(8Z-十四碳烯酰基)甘油酯(Ⅵ)。6个化合物均为首次从该种海藻中得到，Ⅰ和Ⅵ分别为新化舍物和新天然产物，Ⅰ～Ⅲ具有诱导稻瘟霉菌丝变形活性和肿瘤细胞毒性。     

太平洋侧花海葵中四个新神经酰胺的鉴定

目的研究太平洋侧花海葵(Anthopleura pacifica)中的生物活性成分.方法采用稻瘟霉模型生物活性追踪方法,应用色谱技术进行分离,根据理化性质和波谱分析鉴定结构.结果得到了1个对稻瘟霉显示活性的组分,并鉴定为4个神经酰胺的混合物:N-羟乙基-N-十四酰-(2S,3R)-十八碳鞘氨醇-4(E),8(E)-二烯(a),N-羟乙基-N-(9Z-十六烯酰)-(2S,3R)-十八碳鞘氨醇-4(E),8(E)-二烯(b),N-羟乙基-N-十六酰-(2S,3R)-十八碳鞘氨醇-4(E),8(E)-二烯(c)和N-羟乙基-N-(13Z-二十二烯酰)-(2S,3R)-十八碳鞘氨醇-4(E),8(E)-二烯(d).结论化合物a～d均为新的神经鞘氨醇化合物.     

栾树种子的化学成分研究

目的：分离鉴定栾树(Koelreuteria paniculata Laxm.)种子的化学成分。 方法：分别用石油醚回流提取和95% EtOH浸提, 硅胶柱色谱分离, IR,MS,UV,¹HNMR,¹³CNMR等方法确定结构。 结果：分得8个化合物,分别为3/-O-十四烷酰基-1-腈基-2-甲基-1,2-丙烯(1), 3-O-二十碳-14,15-烯酰基-1-腈基-2-甲基-1,2-丙烯(2), 3-O-二十碳-14,15-烯酰基-4-O-十八烷酰基-1-腈基-2-氧代亚甲基-1,2-丙烯(3), 3-O-(6′-亚油酰基-葡萄糖)-β-谷甾醇(4), 1-O-β-D-葡萄糖-2-O-油酸-3-O-十六烷酸甘油酯（5), 1-O-十六烷酸甘油酯（6), 14,15-二十碳烯酸（7),三油酸甘油酯（8)。 结论：1～3为新化合物, 4～8系首次从该植物中分得, 并归属其波谱信号。     

日香桂花的化学成分研究

目的 研究木犀科木犀属植物日香桂干燥花的化学成分.方法 采用柱色谱法分离纯化单体化合物,通过波谱鉴定其结构.结果 分离并鉴定出9个化合物为类叶升麻苷(Ⅰ),Ligustroside(Ⅱ),10-acetoxyligustroside(Ⅲ),齐敦果酸(Ⅳa),乌苏酸(Ⅳb),ursolaldehyde(Ⅴ),dammara-23-en-3β,25一diol(Ⅵ),olean-12-en-3β,27-diol(Ⅶ)和α-O-十六碳烷酰基-B-O-(9Z-十八碳烯酰基)-α-O-十六碳烷酰基甘油酯(Ⅷ).结论 所有化合物均为首次从该植物中得到;化合物Ⅴ-Ⅷ为首次从该属植物中分离得到.     

褐藻叶托马尾藻中的甘油酯类成分

采用稻瘟霉模型生物活性追踪方法，应用多种层析手段，从褐藻叶托马尾藻（Sargassum carpophyllum)中分离得到5个甘油酯类成分，应用多种波谱技术和化学方法，分别确定为：1－O十六碳酰基－3－O－（13Z－十八碳烯酰基）甘油酯（I）1，2，3－O－三910Z，13Z－十八碳二烯酰）甘油酯（II），（2S）－1－O－十六碳酰基2－O－十四碳酰基－3－O－β－D－吡喃半乳糖基甘油（Ⅲ），（2S）－1－O－十六碳酰基－2－O－十六碳酰基－3－O－β－D－吡喃半乳糖基甘油（Ⅳ）和（2S）－1－O－十六碳酰基－2－O（11Z－十八碳烯酰基）－3－O－β－D－吡喃半乳糖基甘油（V），5个化合物均为首次从该各频繁 藻中得到，I和V为新天然产物，Ⅱ-Ⅴ具有诱导稻瘟霉菌丝变形活性和弱的肿瘤细胞毒性。     

太平洋侧花海葵中的化学成分(Ⅲ)

目的　研究太平洋侧花海葵的活性成分。方法　采用稻瘟霉模型生物活性追踪方法 ,应用多种色谱技术进行分离 ,理化性质和波谱数据鉴定其结构。结果　分离并鉴定了 9个化合物 :正十六烷 ( ) ,正十三醇 ( ) ,棕榈酸 ( ) ,硬脂酸 ( ) ,棕榈酸甲酯 ( ) ,花生酸甲酯 ( ) ,1 - O-蜡酰甘油酯 ( )以及 2 S,3 R,Δ4 (E) ,Δ8(E) -十八碳鞘氨醇 -正十六碳酰胺和其同系物 2 S,3 R,Δ4 (E) ,Δ8(E) -十八碳鞘氨醇 -正十四碳酰胺的混合物 ( 和 ) ,其中 和 的混合物对稻瘟霉的最小菌丝变形浓度为 6 3 mg· L-1。结论　 9个化合物均为首次从该种海葵中获得 ,( 和 )具有诱导稻瘟霉菌丝变形活性     

刺松藻和粗枝软骨藻脂溶性化学成分GC-MS分析

目的分析刺松藻和粗枝软骨藻脂溶性化学成分,为其开发利用提供理论依据。方法采用乙醚提取刺松藻和粗枝软骨藻脂溶性成分并用气相色谱-质谱-计算机联用技术对其进行分析。结果从刺松藻中分离鉴定出10个化合物,它们占总脂的92.15%,其主要成分为十四烷酸(4.43%)、正十六烷酸(33.01%)、植醇(8.18%)、油酸(7.07%)、十八碳二烯酸(3.69%)、十六碳三烯酸(3.12%)和豆甾醇(28.83%)等。从粗枝软骨藻中鉴定出16个化合物,占总脂的75.21%,其主要成分为荜澄茄油烯(5.77%)、十四烷酸(1.65%)、正十六烷酸(26.03%)、油酸(5.73%)、十八碳二烯酸(2.24%)、十八碳三烯酸(6.60%)、胆甾醇(7.22%)、胆甾-7,24-二烯-3-醇(11.89%)和豆甾-5,24(28)-二烯-3-醇(3.05%)等。结论饱和脂肪酸、不饱和脂肪酸、甾醇类成分是两种藻的主要成分,刺松藻和粗枝软骨藻脂溶性成分具有很好的药用前景。     

赤芍挥发油成分的GC-MS分析

目的:分析赤芍中挥发油的化学成分,为其质量评价提供科学依据。方法:采用水蒸气蒸馏法提取赤芍挥发油,用气相色谱毛细管柱进行分析,归一化法测定其相对含量,并用气相色谱-质谱联用法鉴定化学成分。结果:检出134个色谱峰,鉴定出35个化合物,占挥发油总量的74.90%。其中n-十六烷酸、(Z,Z)-9,12-十八碳烯酸、油酸、十五烷酸、[1S-(1α,2α,5α)]-6,6-二甲基-二环[3.1.1]庚烷-2-甲醇、十六烷酸乙酯、9,12-十八碳烯酸乙酯、Z-β-松油基苯甲酸脂、1(-2-羟基-4-甲氧基苯基)-乙酮、(R)-1-甲烯基-3(-1-甲基-乙烯基)环己烷是主要成分,占挥发油总量的64.54%。结论:本试验为赤芍挥发油的进一步研究奠定了基础。     

Activity-guided fractionation of the seeds of Ziziphus jujuba using a cyclooxygenase-2 inhibitory assay

Bioactivity-guided fractionation of petroleum ether- and EtOAc-soluble extracts of the seeds of Ziziphus jujuba using a cyclooxygenase-2 assay as a monitor indicated that the triglyceride, 1,3-di-O-[9(Z)-octadecenoyl]-2-O-[9(Z),12(Z)-octadecadienoyl]glycerol (3), and a fatty acid mixture of linoleic, oleic and stearic acids, were the major active components. A new pentacyclic lupane-type triterpene derivative, 3-O-[9(Z)-octadecenoyl]betulinic acid (1), and betulinic acid (2) were also isolated and identified. All isolates as well as pure linoleic, oleic and stearic acids were evaluated for their inhibitory effects against both cyclooxygenases-1 (COX-1) and -2 (COX-2).     

Phytochemical constituens of<Emphasis Type="Italic">Cirsium setidens</Emphasis> Nakai and their cytotoxicity against human cancer cell lines

Five terpenes (1-5), three fatty acids (6-8), two sterols (9 and 11), and a monogalactosyldiacyl glycerol (10) were isolated from the methylene chloride extract of the aerial part of Cirsium setidens. Their chemical structures were determined to be alpha-tocopherol (1), 25-hydroperoxycycloart-23-en-3beta-ol (2), 24-hydroperoxycycloart-25-en-3beta-ol (3), mokko lactone (4), transphytol (5), 9,12,15-octadecatrienoic acid (6), 9,12-octadecadienoic acid (7), hexadecanoic acid (8), acylglycosyl beta-sitosterol (9), (2R)-1,2-O-(9z,12z,15z-dioctadecatrienoyl)-3-O-beta-D-galactopyranosyl glycerol (10) and beta-sitosterol glucoside (11) by spectral evidences. Compound 3 exhibited significant cytotoxic activity against five human cancer cell lines with its ED50 values ranging from 2.66 to 11.25 microM.     

Sulfoglycolipid from the marine brown alga Sargassum hemiphylum.

One kinds of glycolipid (SBI) have been isolated from the marine brown alga Sargassum hemiphyllum (Turn.) Ag. The structures of SBI have been determined as the sodium salt of 1-0-acyl-3-0-(6'-sulfo-alpha-D-quinovopyrannosyl) glycerol (acyl: tetradecanoyl, pentadecanoyl, 11-hexadecenoyl, hexadecanoyl, 10,13-octadecadienoyl, 9-octade cenoyl, 15-metylheptadecanoyl and 11-eicosenoyl 17: 1.5: 19: 153: 1: 19: 1: 2) on the basis of chemical and spectral evidence and GC-MS analysis, respectively. Four constituents of the SBI were new compounds [the sodium salt of 1-0-(11"-hexadecenoyl)-3-0-(6'-sulfo-alpha-D-quinovopyrannosyl) glycerol, the sodium salt of 1-0-(10",13"-octadecadienoyl)-3-0-(6'-sulfo-alpha-D-quinovopyrannosyl) glycerol, and the sodium salt of 1-0-(15"-metylhexadecenoyl)-3-0-(6'-sulfo-alpha-D-quinovopyrannosyl) glycerol, and the sodium salt of 1-0-(11"-eicosenoyl)-3-0-(6'-sulfo-alpha-D-quinovopyrannosyl) glycerol]. All compounds were isolated from marine brown alga for the first time.     

Potentially hepatoprotective glycolipid constituents ofLycium chinense fruits

Further investigation of Lycium chinense fruits gave a mixture of (6'-O-palmitoyl)- and (6'-O-stearoyl)-beta-sitosterol-3-O-beta-D-glucopyranoside (1) and two glycolipids, 1-O-(9Z,12Z, 15Z-octadecatrienoyl)-2-O-(9Z, 12Z, 15Z-octadecatrienoyl)-3-O-beta-D-galactopyranosyl glycerol (2) and 1-O-(9Z, 12Z-octadecadienoyl)-2-O-(9Z, 12Z, 15Z-octadecatrienoyl)-3-O-beta-D-galactopyranosyl glycerol (3). These compounds were newly isolated as constituents of L. chinense.     

光果甘草三萜皂苷类化学成分研究

采用聚酰胺—大孔树脂柱色谱、ODS中压柱色谱以及半制备液相色谱分离技术,对光果甘草(Glycyrrhiza glabra L.)水提取物中三萜皂苷类成分进行分离纯化,共获得10个三萜皂苷类化合物,依据理化性质及NMR、MS波谱数据鉴定化合物结构,分别鉴定为3β-O-[β-D-glucuronpyranosyl-(1→2)-β-D-glucuronpyranosyl]-30β-O-β-Dglucuronpyranosyl-oleanane-11-oxo-12(13)-ene(1)、3β-O-[β-D-glucuronpyranosyl-(1→2)-β-D-glucuronpyranosyl]-30βO-α-L-rhamnopyranosyl-oleanane-11-oxo-12(13)-en-22β,30-diol(2)、uralsaponin C(3)、licorice-saponin A3(4)、licoricesaponin P2(5)、22β-acetoxyl-glycyrrhizin(6)、macedonoside A(7)、29-hydroxyl-glycyrrhizin(8)、licorice-saponin G2(9)和glycyrrhizin(10),其中化合物1和2为新化合物,命名为licorice-saponin R3和licorice-saponin S3。     

Chemical constituents of the whole plant of Elsholtzia rugulosa

A new lignan glycoside, 7α, 7'β-bis-(4-hydroxy-3-methoxyphenyl)-7,9':7',9-diepoxylignan-8αH,8'α-O-β-d-(2″,7'α-epoxy)-glucopyranoside, named elshrugulosain (1), has been isolated from the EtOH extract of Elsholtzia rugulosa, together with six known compounds, ( - )-bornyl (E)-3,4,5-trimethoxycinnamate, apigenin, luteolin, apigenin-7-O-β-d-glucopyranoside, luteolin-7-O-β-d-glucopyranoside, and luteolin-3'-glucuronate methyl ester. Their structures were characterized by spectroscopic methods and comparison with the data in the literature. On the basis of detailed 1D and 2D NMR spectral analysis, as well as X-ray crystallographic diffraction, the NMR spectroscopic data of ( - )-bornyl (E)-3,4,5-trimethoxycinnamate were revised and assigned completely.     

Furostanol oligoglycosides from Asparagus cochinchinensis

Three new furostanol oligoglycosides, named aspacochioside A (1), B (2) and C (3), together with the known compound 3-O-[(alpha-L-rhamnopyranosyl-(1 --> 4))(beta-D-glucopyranosyl)]-26-O[beta-D-glucopyranosyl]-(25S)-5beta-spirostane-3beta-ol were isolated from the roots of Asparagus cochinchinensis. Their structures were elucidated by spectroscopic techniques (IR, HR-ESIMS, ESIMS/MS, ID and 2D NMR) and chemical methods as 3-O-[(alpha-L-rhamnopyranosyl-(1 --> 4))(beta-D-glucopyranosyl)]-26-O-[beta-D-glucopyranosyl]-(25S)-5beta-furostane-3beta,22alpha,26-triol (1), 3-O-[(alpha-L-rhamnopyranosyl-(1 --> 4))(beta-D-glucopyranosyl)]-26-O-[beta-D-glucopyranosyl]-22alpha-methoxy-(25S)-5beta-furostane-3beta,26-diol (2), and 3-O-[(alpha-L-rhamnopyranosyl-(1 --> 4))(beta-D-glucopyranosyl)]-26-O-[beta-D-glucopyranosyl]-(25S)-5beta-furost-20(22)-en-3beta,26-diol (3).     

Two new anthraquinone glycosides from the roots of Rheum palmatum

Two new anthraquinone glycosides, named 1-methyl-8-hydroxyl-9,10-anthraquinone-3-O-β-D-(6'-O-cinnamoyl)glucopyranoside (1) and rhein-8-O-β-D-[6'-O-(3'-methoxyl malonyl)]glucopyranoside (2), have been isolated from the roots of Rheum palmatum, together with seven known compounds, rhein-8-O-β-D-glucopyranoside (3), physcion-8-O-β-D-glucopyranoside (4), chrysophanol-8-O-β-D-glucopyranoside (5), aleo-emodin-8-O-β-D-glucopyranoside (6), emodin-8-O-β-D-glucopyranoside (7), aleo-emodin-ω-O-β-D-glucopyranoside (8), and emodin-1-O-β-D-glucopyranoside (9). Their structures were elucidated on the basis of chemical and spectral analysis.     

Chemical constituents from the red alga Symphyocladia latiuscula

The extraction and solvent fractionation of red alga Symphyocladia latiuscula, and repeated column chromatography led to isolation of 17 compounds: cholesterol (1), 3β,5β-dihydroxy-B-norcholestan-6β-carboxaldehyde(2),6β-hydroxy-cholest-4-ene-3-one(3), 1-O-myristoyl-3-O-(6′-sulfo-α-D-quinovopyranosyl)glycerol (4), 1-O-palmitoyl-3-O-(6′-sulfo-α-D-quinovopyranosyl)glycerol (5), 1-O-palmitoyl-3-O-[α–D-galactopyranosyl(1→6)β-D-galactopyranosyl] glycerol(6), 1-O-palmitoyl-3-O-β-D-galactopyranosylglycerol (7), methyl hexadecanoate (8), methyl stearate(9), hexadecanoic acid (10), γ-n-butyl cis-aconiate (11), α-n-Butyl cis-aconiate (12), phenethylamine (13),3,5-dibromo-L-tyrosine (14), 3-methylbutylamine (15),methyl pyroglutamate (16),n-butyl pyroglutamate (17).The structures of these compounds were identified by NMR spectroscopy and mass spectrometry, and compared with those reported in the literature. All the compounds were isolated from Symphyocladia genus for the first time.These compounds were investigated for their inhibitory effects on human recombinant aldose reductase in vitro. Of the compounds, 1-O-palmitoyl-3-O-β-D-galactopyranosylglycerol (7) demonstrated moderate enzyme inhibition.