共查询到20条相似文献,搜索用时 93 毫秒
1.
2.
3.
4.
为了探讨败酱草多糖的最佳提取工艺条件,采用苯酚-硫酸法法测定多糖的含量,以多糖得率为试验指标,对影响多糖提取率的料液比、温度、提取时间、提取次数等因素分别进行单因素试验,在单因素试验的基础上,进行L9(34)正交试验,通过极差分析得出败酱草多糖的最佳提取工艺条件.结果表明,影响败酱草多糖提取率的最主要因素是温度,败酱草多糖的最佳提取工艺条件为温度90℃、料液比1∶20、时间3h、提取次数3 次,在此工艺条件下,提取败酱草多糖得率为3.781%. 相似文献
5.
6.
《中国药房》2015,(19):2695-2697
目的:优化对叶百部粗多糖提取工艺,提高其提取率及效率。方法:采用蒽酮-硫酸法检测,分别以水及碱水(Na OH溶液)为溶剂,考察煎煮法、超声波法和微波法对对叶百部粗多糖提取率的影响;采用单因素及正交试验,以粗多糖提取率为指标,考察加水量、Na OH溶液浓度、提取温度、提取时间对碱水超声法提取粗多糖的影响,优化其工艺参数并进行验证试验。结果:碱水超声波法优于其他提取方法;其最优工艺参数为加入药材量30倍的0.3 mol/L Na OH水溶液,50℃超声处理70 min;验证试验结果表明粗多糖平均提取率为25.76%(RSD=3.51%,n=6)。结论:优选的碱水超声波法可较好地提取出对叶百部中粗多糖成分。 相似文献
7.
目的:测定不同产地刺五加不同部位的多糖含量。方法:采用微波提取法提取刺五加中的多糖,考察微波提取时间、料液比、提取功率3个因素对多糖提取率的影响,确定最佳提取条件,并采用苯酚一硫酸法测定其含量。结果:微波提取法提取刺五加中多糖的最佳条件为提取功率700W,料液比1:30,作用时间120S;刺五加根部及茎部多糖含量均以本溪产最高,穆枝产次之,珲春产最低。结论:用微波提取法提取刺五加中的多糖具有快速、高效、节能、不破坏有效成分等特点,具有良好的应用前景;苯酚一硫酸法简便、快速、准确,可作为刺五加多糖的含量测定方法;不同产地、不同部位刺五加药材的多糖成分的含量不同。 相似文献
8.
茯苓多糖具有抗病毒、抗肿瘤、抗氧化、增强机体免疫力、降血糖、降血脂等作用.目的:提高茯苓提取率.方法 参考国内部分文献,以茯苓多糖为考察指标,对茯苓多糖近几年的提取工艺的进行比较总结.结果 普通水提醇沉价廉、无毒、并且操作安全.但提取率较低,浸提的时间长;酶解+热水浸提为普通热水浸提法的2.32倍;稀碱浸提法提取率较高,但是极易抑制茯苓多糖的生物活性;微波提取法能显著提高茯苓多糖的提取率,而且微波提取法能保持茯苓原有营养成分,并且提取速度快、操作便捷;超声波提取法降低了能量的消耗,从而提取率更高、提取时间更短.结论 超声波提取法为最理想的茯苓多糖的提取方式. 相似文献
9.
目的研究酶法提取首乌藤多糖的最佳工艺条件。方法利用酶解法提取首乌藤多糖,筛选出最佳适用酶,然后利用苯酚-硫酸法测定多糖含量,以多糖得率和多糖含量为综合评价指标,采用单因素试验对影响首乌藤多糖的提取工艺进行研究。结果首乌藤多糖的最佳适用酶为纤维素酶,由单因素试验确定的酶法提取首乌藤多糖的最佳工艺条件为pH 5.0,纤维素酶用量3%,酶解时间4 h,酶解温度55℃。结论纤维素酶可显著提高首乌藤多糖的提取率。 相似文献
10.
11.
除莠霉素A结构类似物M3、M4的分离鉴定及对肿瘤细胞的活性 总被引:2,自引:1,他引:1
吸水链霉菌NND-52发酵液的上清液经乙酸乙酯萃取,硅胶柱粗分离,经制备型高效液相色谱仪分离纯化,得除莠霉素A(herbimycin A)、黄色M3纯品和浅黄色M4粗品,粗品结晶后获得M4的白色针状结晶;通过基本理化性质和光谱分析,确定M3、M4分别为除莠霉素C(herbimycin C)、双氢除莠霉素A;噻唑蓝(MTT)法检测除莠霉素A、除莠霉索C及双氢除莠霉素A对Veto、B16-F10细胞增殖的影响,对肿瘤细胞活性强度顺序为:除莠霉素C〉除莠霉素A〉双氢除莠霉素A。除莠霉素C可能是一种更具潜力的抗肿瘤抗生素。 相似文献
12.
白花瓜叶乌头中生物碱成份的研究 总被引:3,自引:0,他引:3
目的:研究白花瓜叶乌头(Aconitum hemsleyanum Pritz vor.laueanthus P.Guo et.M.R.Jia)根中生物碱成份。方法:pH与溶剂极性梯度萃取及硅胶层析。结果:从中分得7个已知去甲二萜生物碱,由光谱法分别鉴定为大渡乌碱1;13,15-dideoxyaconitine 2;indaconitine 3;crassicaudine4;塔拉萨敏5;查斯曼宁6;ezochasmanine 7。结论:这些化合物均首次从该植物中分离得到。 相似文献
13.
Arai M Tomoda H Matsumoto A Takahashi Y Woodruff BH Ishiguro N Kobayashi S Omura S 《The Journal of antibiotics》2001,54(7):554-561
Streptomyces sp. WK-6326, a soil isolate, was found to produce an inhibitor of interleukin (IL)-4 signal transduction. Two structurally related compounds, a novel one designated deacetylravidomycin M and the known deacetylravidomycin, were isolated from the culture broth by solvent extraction, silica gel column chromatography and HPLC. Deacetylravidomycin M inhibited IL-4-induced CD23 expression in U937 cells without any cytotoxic effect, whereas deacetylravidomycin showed no inhibitory activity. 相似文献
14.
J. VANGGAARD ANDERSEN K. T. HANSEN 《Xenobiotica; the fate of foreign compounds in biological systems》2013,43(9):901-912
1. The metabolism of Odapipam has been studied with phenobarbital-induced rat liver microsomes, followed by analysis with normal-phase hplc in combination with particlebeam mass spectrometry. 2. During the incubation of Odapipam, five different metabolites were formed. The EI mass spectra of the metabolites indicated the formation of N-desmethyl-Odapipam, 1-hydroxy-Odapipam, the two isomers of 3′-hydroxy-Odapipam and a metabolite which was dehydrogenated in the dihydrobenzofuran moiety. 3. The intrinsic hepatic extraction ratio and metabolism of Xanomeline has been studied in the perfused rat liver. Increasing the input concentration resulted in measurable concentrations of Xanomeline in the perfusate, although the extraction ratio was still > 0·9 at 140 μM. 4. Analysis of the perfusate by normal-phase hplc and particle-beam mass spectrometry showed the formation of at least six metabolites. The EI+ mass spectrum of the metabolites indicated the formation of ω-3 hydroxy-, ω-2 hydroxy-, ω-1 hydroxy-, ω-1 ketoXanomeline in addition to ω-1 hydroxy-N-desmethyl-Xanomeline and an N-oxide of Xanomeline. 5. The results show that normal-phase hplc based on silica material is superior to reversed-phase-based systems in terms of selectivity. Furthermore, the use of non-aqueous solvents in combination with particle-beam mass spectrometry is advantageous compared with reversed-phase hplc since changing between different solvents in normal-phase hplc results only in minor changes in the particle-beam interface parameters such as nebulizer position, helium pressure and interface temperature. 相似文献
15.
目的比较研究微波萃取法和索氏提取法对阿育魏实挥发油的提取工艺。方法以得油率为指标,液料比、蒸馏时间、提取时间、碎粒度为提取因素进行正交试验得出最佳工艺。结果索氏提取法当取样量为20 g、提取时间为4 h、碎粒度为粉碎状、虹吸速度为中速时为最佳提取条件;而微波法当取样量为25 g、微波时间为90 s、浸泡时间为30 min、碎粒度为粉碎状,即为最佳提取条件。结论与索氏法比较,微波法节约能耗,提取速率快,适合大量快速提取。 相似文献
16.
目的:探讨超声波法和索式提取法对一点红中总生物碱的最佳工艺条件与测定方法的研究。方法:以紫外分光光度法为测定方法,一点红中总生物碱提取来量为考察目标,采用正交实验法优化提取工艺。结果:不同温度、不同浓度、提取时间对一点红总生物碱提取量有显著影响。提取次数影响不大。结论:超声波法的提取最佳工艺为80℃下以物料比为1∶40对一点红生物碱类物质进行30min提取,得出提取率为0.435%,索式提取法的提取最佳工艺为用80%的乙醇对一点红生物碱类物质进行2h提取,得出提取率为0.320%。通过两种方法的比较,一点红总生物碱的提取为超声波法更优。 相似文献
17.
18.
制药工业中溶剂萃取技术的机制和应用发展方向 总被引:11,自引:1,他引:10
制药工业中溶剂萃取技术的机制和应用发展方向李洲,廖史书,雷文(清华大学化工系,国家医药管理局清华大学医药工程技术研究中心,北京100084)溶剂萃取技术在制药工业中已有广泛应用,但仍具有极大的开发潜力[1~4],青霉素的溶剂萃取就是一个典型的例子。溶... 相似文献
19.
20.
Nikola Maravić Nemanja Teslić Dora Nikolić Ivana Dimić Zita Šereš Branimir Pavlić 《Sustainable Chemistry and Pharmacy》2022
Different conventional and “green” extraction techniques (solid/liquid extraction, microwave and ultrasound assisted extraction, pressurized liquid extraction and subcritical water extraction) were tested using different operating conditions in the recovery of valuable polyphenol compounds from sugar beet leaves. All tested extraction techniques provided considerable extraction yield in the range from 18.21% to 37.04%. Total phenolic content ranged from 0.4504 up to 1.7171 (g GAE/100 g DW) with the highest values obtained by using microwave and ultrasound assisted extraction. Extraction parameters had highly significant effect on the concentration of bioactive compounds recovered by pressurized liquid extraction and subcritical water extraction. Individual phenolic profile of sugar beet leaves extracts largely depended on the applied extraction technique, however, vitexin was the most abundant phenolic compound present in all extracts regardless of the extraction technique. Investigation of extracts antioxidant activity through DPPH, FRAP and ABTS values indicated the lowest antioxidant activity of extracts obtained by solid/liquid extraction compared to other extraction techniques. 相似文献