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1.
采用聚酰胺薄膜色谱法鉴别荔大前合剂中荔枝草,展开剂为甲醇-乙酸-水(65:15:20)。用高效液相色谱法测定,以ODS(250mm×4.6mm,5μm)为分析柱,甲醇-磷酸盐缓冲液(45:55)为流动相;检测波长为335nm柱温45℃,用面积外标法。结果表明,根据聚酰胺薄膜色谱图可鉴定制剂中荔枝草。当高车前苷在3.36×10~(-3)~0.215μg范围内呈线性。平均回收率为98.8%,RSD为2.14%。  相似文献   

2.
高效液相色谱法测定复方荔枝草颗粒剂中高车前苷含量   总被引:4,自引:0,他引:4  
目的 :建立测定复方荔枝草颗粒剂中高车前苷含量的高效液相色谱法。方法 :以KF -C18(2 5 0mm× 4 6mm ,10 μm)为分析柱 ,流动相为甲醇 -水 -pH 3 5的 1 0mol·L-1磷酸盐缓冲液 (4 0∶5 5∶5 ) ;柱温为 45℃ ;检测波长为 335nm ;流速为 1 0mL·min-1,采用面积外标法。结果 :线性范围 3 12× 10 -3 ~ 0 2 0 μg ,平均回收率为 96 96 %~ 10 1 5 % ,RSD <0 6 9% (n =3)。结论 :本法简便、灵敏、重现性好 ,可用于测定该颗粒剂高车前苷的含量和质量标准的控制。  相似文献   

3.
目的 建立高效液相色谱法鉴别利福平滴眼液。方法 采用Spherisorb CN色谱柱(4.6mm×250mm,5μm) ;流动相:甲醇-乙腈-0.075mol·L-1磷酸二氢钾溶液 (80∶80∶65);流量 1ml·min-1;检测波长254nm;进样体积为20μl。结果 具有良好的重复性。结论 本法专属性强。  相似文献   

4.
高效液相色谱法测定苯磺酸氨氯地平分散片的含量   总被引:1,自引:0,他引:1  
目的 用高效液相色谱法测定苯磺酸氨氯地平分散片的含量。方法 采用ODS色谱柱(4.6mm×250 mm,5μm,phnomenex),甲醇-0.03 mol·L-1磷酸二氢钾溶液(65∶35)为流动相,检测波长为237 nm。结果 苯磺酸氨氯地平在49.34μg·ml-1~91.62μg·ml-1浓度范围内,峰面积与浓度呈良好的相关性,相关系数为0.9996,平均回收率为99.91%,RSD为0.76%,平均含量为101.6%,RSD为0.56%(n=6)。  相似文献   

5.
曹琳  钱江 《中国药师》2014,(8):1316-1318
摘 要 目的:建立高效液相色谱法测定布洛伪麻缓释片释放量的方法。方法: 色谱柱为迪马 C18(250 mm×4.6 mm,5 μm),流动相为甲醇-三乙胺-0.04 mol·L-1 磷酸二氢钠溶液 (60∶0.02∶40),流速为1.0 ml·min-1,检测波长为215 nm,柱温为35℃,进样量为20 μl。结果:布洛芬的线性范围为40~2 000 μg·mL-1(r=1.000 0),平均加样回收率为99.2%,RSD=0.8%(n=9),最低定量浓度为0.04μg· mL-1;盐酸伪麻黄碱的线性范围为6~300 μg·mL-1(r=1.000 0),平均加样回收率为98.5%,RSD=0.6%(n=9),最低定量浓度为0.06μg· mL-1。结论: 该方法简便快速、准确灵敏,可用于布洛伪麻缓释片释放量的测定。  相似文献   

6.
目的建立测定复方荔枝草颗粒剂中桃叶珊瑚苷含量的高效液相色谱法.方法色谱柱为KF-C18柱(250mm×4.6mm,10tμm),流动相为甲醇-水-磷酸(30969.50.5);柱温为35℃;检测波长为203nm;流速为1.0mL@min1.结果线性范围0.05~0.80μg,平均回收率为84.46%,RSD为1.37%(n=9).结论本法简便、灵敏、重现性好,可作为该制剂的质量控制标准.  相似文献   

7.
建立头孢呋辛酯及其片剂的含量测定方法。以岛津Shim-Pack C18(4.6 mm×150 mm,5μ)色谱柱,以0.2 mol/L磷酸二氢铵溶液-甲醇(62:38)为流动相,以278 nm为检测波长。线性范围为0.05~2 mg/ml,回归方程为Y=2.69×106X-1.87×105,r=0.9999,平均回收率为99.4%(RSD=0.9%,n=9)。  相似文献   

8.
目的:建立曲安奈德新霉素贴膏处方中麝香草酚的含量测定方法。方法:首先,通过正己烷溶解膏剂,90%乙醇萃取的方法提取贴膏剂中的麝香草酚,然后建立高效液相色谱法测定其含量,色谱条件为: Kromasil C18色谱柱(250 mm×4.6 mm,5 μm),柱温:30℃,流动相:甲醇-0.2 %乙酸(45:55),流速:1.0 ml.min-1,进样量:20μl,检测波长:276 nm。  相似文献   

9.
罗湘  曾繁典 《药学学报》1995,30(8):605-609
介绍了一种测定人血清盐酸劳卡尼(lorcainide hydrochloride)浓度的高效液相色谱法(HPLC)。不锈钢分析柱(200mm×5mmID)固定相为YWG C18H37,5μm颗粒。流动相为甲醇-水-0.625mol·L-1醋酸铵(86:13:1v/v),用浓氨水调至pH8.0。流速1ml·min-1。取地尔硫(diltiazem)为内标物。紫外检测波长226nm。低、中、高3种浓度方法回收率分别为95.85%,100.63%,100.09%,由低至高四种浓度日内、日间RSD小于7%。血清最低检测浓度为5μg·L-1。在20~800μg·L-1浓度范围内线性良好,r=0.9996。  相似文献   

10.
目的:建立双嘧达莫片中有关物质的方法。方法:采用高效液相色谱法,色谱柱为Agilent XDB-C18(4.6mm×250mm,5μm),流动相为0.1%磷酸氢二钠溶液(用磷酸调节pH值至4.6)-甲醇(25:75),检测波长为288nm,流速1.0mL.min-1。结果:双嘧达莫与其降解产物在该色谱条件下能够有效分离。结论:所建方法简便,专属性强,可以用于双嘧达莫片中有关物质的测定。  相似文献   

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13.
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.  相似文献   

14.
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.  相似文献   

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16.
Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.
  相似文献   

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18.
This article assesses pain within the context of the dose response. A substantial number of studies indicate that the dose response for pain-related endpoints is commonly biphasic, being independent of the type of biological model employed, endpoint measured, or agent tested. The quantitative features of the dose response are also remarkably consistent regardless of the receptor pathway that mediates the nociceptive response, indicating a likely downstream message convergence. These findings have important implications for drug discovery, development, and clinical evaluation.  相似文献   

19.
Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren.  相似文献   

20.
The current USP National Formulary contains 65 Monographs for drug formulations containing neomycin. All 65 Monographs prescribe a bioassay for neomycin assay. This bioassay, based on cell culture, is labor intensive, has poor precision, and cannot be adapted for purity or identification. High-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPAE-IPAD), a liquid chromatography technique, has been shown to be suitable for neomycin purity analysis and neomycin assay of an over-the-counter first aid cream (Hanko and Rohrer [17]). Here we propose that an HPAE-IPAD assay can replace the bioassay in the 65 neomycin-containing Monographs. We applied the HPAE-IPAD assay to four neomycin-containing drug products representing the four classes of formulations found in the 65 Monographs, liquid, solid, suspension, and cream. Each drug was analyzed with two chromatography systems, and on 3 separate days. For all products, HPAE-IPAD measurements were precise and accurate with respect to the label concentrations. There was also high accuracy for spike recovery of neomycin from the four drug products throughout 70–150% of the labeled concentration. These results suggest that an HPAE-IPAD assay would be an accurate assay for neomycin, and would be faster and more precise than the current bioassay.  相似文献   

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