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1.
血小板活化因子测定诊断肝纤维化疾病的临床意义   总被引:2,自引:0,他引:2  
目的:研究血小板活化因子测定与肝纤维化疾病的临床意义。了解肝脏受损在肝纤维化患者微循环改变中的作用。方法:采用固相免疫放射法检测各期肝纤维化患者和正常人血浆血小板活化的血栓素(TXB2)、6-酮-前列腺素(6-K-PGF1α)、α-颗粒膜蛋白(GMP-140)这三项指标的水平。结果:肝纤维化患者TXB2、GMP-140、TXB2/6-K-PGF1α的水平较正常人显著升高,6-K-PGF1α显著下降。结论:各期肝纤维化患者存在不同程度微循环障碍,肝纤维化程度越重TXB2、GMP-140、TXB2/6-K-PGF1α值越大,6-K-PGF1α值越小,微循环障碍越重,检测以上几项指标可为临床肝纤维化提供辅助诊断依据,使肝纤维化患者及早得到诊断,预防肝硬化。  相似文献   

2.
目的:探讨冠心病(CHD)患者血清血栓素B2(TXB2)、6-酮-前列腺素F1α(6-K-PGF1α)水平和凝血酶原时间国际标准化比率(PTINR)的变化及阿司匹林对其的干预影响。方法:采用放射免疫法测定了30例CHD患者和20例非冠心病患者的血清TXB2、6-K-PGF1α水平,生化法测定PTINR,对CHD组患者给予阿司匹林干预治疗,2周后复查上述指标,并进行对照分析。结果:CHD组血清TXB2水平和PTINR显著高于对照组(P均<0.01),6-K-PGF1α水平低于对照组(P<0.01)。TXB2与PTINR呈相关(r=0.413,P<0.01),TXB2与6-K-PGF1α及PTINR与6-K-PGF1α间均无显著相关性(P均>0.05)。经平均22.3日的阿司匹林(益欣雪)干预治疗后,CHD组血清TXB2水平和PTINR显著下降(P<0.05),但6-K-PGF1α水平无明显改变(P>0.05)。结论:CHD患者存在着血清TXB2、6-K-PGF1α水平和PTINR变化,此与其发生、发展相关,阿司匹林的干预治疗有助于此状态的改善。  相似文献   

3.
鲨鱼软骨制剂抗肿瘤作用的研究   总被引:16,自引:0,他引:16  
以鲨鱼软骨为原料,经盐酸胍抽提,丙酮分级沉淀,超滤,Sephadex G-75柱层折等步骤得到鲨鱼软骨制剂 1(SP1)和鲨鱼软骨制剂 2(SP2)。测定它们对肿瘤细胞和血管内皮细胞DNA合成的抑制效应,对小鼠移植瘤的抑癌作用,以及对小鼠脾脏和胸腺的影响。结果表明:SP1能同时抑制血管内皮细胞和直接抑制肿瘤细胞;SP2同时具有提高机体免疫功能、直控抑制肿瘤细胞和抑制血管内皮细胞的活性。整体实验说明它们都具有较强的抑制肿瘤生长的活性,对小鼠体重无明显影响,无明显毒副作用,显示了良好的应用前景。  相似文献   

4.
目的:观察中药肠瑞灌肠剂对放射性直肠炎大鼠血浆6-eto—PGF1α及TXB2的影响。方法:利用直线加速器建立放射性直肠炎大鼠模型,随机分为肠瑞灌肠剂治疗组、中药及化学药阳性对照组、模型和空白对照组,治疗7天后处死,放免法检测各组血浆6-酮-前列腺素(6-keto—PGF1α)、血栓素B2(TXB2)的含量。结果:照射后各组6-Kote—PGF1α均有不同程度下降,而TXB2以及TXB2/6-Kote—PGF1α均有不同程度升高,肠瑞灌肠剂能上调放射性直肠炎大鼠血浆6-Kote—PGF1α含量,下调其TXB2含量及TXB2/6-Kote—PGF1α比值。结论:肠瑞灌肠剂可改善放射性直肠炎局部组织血液循环,抑制血小板活化,从而减轻炎症反应,促进肠黏膜修复。  相似文献   

5.
黄展  辛华  江旭东 《肿瘤药学》2013,(6):432-435
目的探讨独角莲醇提液对H22肝癌荷瘤小鼠血清IL-2和TNF-α水平的影响。方法建立体内H22肝癌荷瘤小鼠的模型,采用灌胃法给予其100mg·kg^-1·d^-1独角莲醇提液,以生理盐水灌胃和5-FU腹腔注射作为对照,连续10d,停药后第2天摘取小鼠眼球取血后断髓处死,取出胸腺、脾脏称重,计算小鼠的胸腺重量指数、脾脏重量指数,采用ELISA法检测小鼠血清中细胞因子白介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)的水平。结果与生理盐水灌胃的H22肝癌荷瘤小鼠比较,5-FU腹腔注射和独角莲醇提液灌胃处理的H22荷瘤鼠血清中细胞因子IL-2、TNF-α水平明显升高;但胸腺重量指数、脾脏重量指数显著下降,差异均具有统计学意义(P〈0.05)。结论独角莲醇提液可提高H22荷瘤鼠的血清IL-2和TNF-α水平。  相似文献   

6.
老年患者一旦确诊为冠心病,就要长期坚持服药,如抗血小板药物、他汀类药物ACEI或ARB类药物,还可以酌情应用硝酸酯类药物及钙离子拮抗剂药物等,此类药物可以升高患者的6酮前列环素F1α(6Keto—PGF1α)、NO水平,降低血栓素B2(TXB2)、ROS,阻止前列腺素代谢紊乱;这与其改善患者血管内皮细胞功能关系密切,尤其是针对气虚血瘀证冠心病患者,所以,测定冠心病不同病期TXB2、6Keto—PGF1α、NO、ROS浓度水平临床意义重大。  相似文献   

7.
疏血通注射液对脑梗死患者TXB_2及6-Keto-PGF1a的影响   总被引:1,自引:0,他引:1  
目的:探讨疏血通注射液对急性脑梗死患者血浆6 酮 前列环素(6 Keto PGF1a)、血栓素B2 (TXB2 )的影响。方法:6 0例急性脑梗死患者随机分为两组,对照组30例进行常规基础治疗,治疗组30例在常规基础治疗的同时给予疏血通注射液静脉滴注,治疗前后比较两组患者神经功能评分、TXB2 、6 Keto PGF1a的变化。结果:治疗组神经功能评分明显减少、TXB2 降低、6 Keto PGF1a升高,与对照组比较有明显差异。结论:疏血通注射液可调节TXB2 / 6 Keto PGF1a的平衡,抑制血小板活化,发挥一定的抗血栓作用  相似文献   

8.
目的和方法:观察固脱汤对失血性休克大鼠血浆NO、6-keto-PGF1α和TXA2含量的影响。结果:失血性休克大鼠血浆NO水平增高(P<0.01),6-keto-PGF1α含量明显降低(P<0.01),TXA2含量增高(P<0.01),固脱汤加可明显升高血浆NO、6-keto-PGF1α水平(P<0.01),而血浆TXB2含量未见显著性改变(P>0.05)。结论:固脱汤可通过促进血管内皮细胞产生和释放NO及PGI1发挥抗休克作用。  相似文献   

9.
大鼠脾不统血模型血浆和子宫6—K—PGF1α、TXB2变化的研究   总被引:3,自引:0,他引:3  
目的:观察比较脾气虚证模型、脾不统血证模型与单纯出血因素及卵巢摘除大鼠血浆、子宫PG的变化。方法:以放射免疫法检测上述模型大鼠血浆、子宫6-酮-前列腺素F1α(6-K-PGF1α)和血栓素2(TXB2)。结果:各种造模因素均导致血浆、子宫6-K-PGF1α/TXB2升高,机体表现为出血倾向。结论:6-K-PGF1α/TXB2比值升高可能是脾不统血证出血的机理之一。  相似文献   

10.
齐东江  徐阿曼 《安徽医药》2015,19(9):1665-1667
目的:研究长期使用奥美拉唑( omeprazole,OME)是否会抑制小鼠体内溶酶体及其对小鼠免疫器官的影响。方法实验分为单纯对照组、OME低剂量组(6 mg· kg-1)、OME高剂量组(30 mg· kg-1),每组10只小鼠。饲养24周后处死小鼠, ELISA法检测小鼠血清及脾脏中酸性磷酸酶( acid phosphatase,ACP)、N-乙酰-β-D-氨基葡萄糖苷酶( N-acetyL-β-D-glucosamini-dase,NAG)含量,采集小鼠胸腺、脾脏并称重计算小鼠脾脏、胸腺重量指数。结果 OME组与对照组相比血清中溶酶体酶活性下降,两者差异具有统计学意义(P<0.05),同时小鼠脾脏及胸腺重量指数明显下降。结论 OME可以抑制小鼠体内溶酶体及其水解酶的活性,导致系统性免疫功能受损,从而降低小鼠免疫功能。  相似文献   

11.
胎盘免疫调节肽的抗肿瘤作用   总被引:7,自引:3,他引:4  
用S180、结肠癌26及FC瘤细胞分别接种于小鼠,建立肿瘤动物模型,然后用胎盘免疫调节肽进行治疗,观察其抑瘤效应。体内实验结果表明:胎盘免疫调节肽可明显抑制S180和结肠癌26瘤细胞的生长,其抑瘤率分别为48%和57%;明显抑制FC瘤细胞的转移。  相似文献   

12.

Aim:

IL-37b has shown anti-cancer activities in addition to its anti-inflammatory properties. In this study, we investigated the effects of IL-37b on breast carcinoma growth in mice and to determine the involvement of T cell activation in the effects.

Methods:

IL-37b gene was transferred into mouse breast carcinoma cell line 4T1 (4T1-IL37b cells), the expression of secretory IL-37b by the cells was detected, and the effects of IL-37b expression on the cell proliferation in vitro was evaluated. After injection of 4T1 cells or 4T1-IL37b cells into immunocompetent BALB/c mice, immunodeficient BALB/c nude mice and NOD-SCID mice, the tumor growth and survival rate were measured. The proliferation of T cells in vitro was also detected.

Results:

IL-37b was detected in the supernatants of 4T1-IL37b cells with a concentration of 12.02±0.875 ng/mL. IL-37b expression did not affect 4T1 cell proliferation in vitro. BALB/c mice inoculated with 4T1-IL37b cells showed significant retardation of tumor growth. BALB/c mice inoculated with both 4T1 cells and mitomycin C-treated 4T1-IL37b cells also showed significant retardation of tumor growth. But the anti-cancer activity of IL-37b was abrogated in BALB/c nude mice and NOD-SCID mice inoculated with 4T1-IL37b cells. Recombinant IL-37b slightly promoted CD4+ T cell proliferation without affecting CD8+ T cell proliferation.

Conclusion:

IL-37b exerts anti-4T1 breast carcinoma effects in vivo by modulating the tumor microenvironment and influencing T cell activation.  相似文献   

13.
Anti-invasive and metastatic activities of evodiamine   总被引:8,自引:0,他引:8  
We have recently reported that evodiamine can suppress in vitro invasion and lung metastasis by colon 26-L5 carcinoma cells. To extend our study, we examine here the anti-invasive and metastatic effects of evodiamine on Lewis lung carcinoma (LLC) and B16-F10 melanoma in addition to colon 26-L5 carcinoma. Critical structures of evodiamine for the activities were also evaluated by comparison with compounds possessing structures similar to that of evodiamine. Evodiamine concentration-dependently inhibited the invasion of B16-F10, LLC and colon 26-L5 cells with IC(50) values of 2.4 micro M, 4.8 micro M and 3.7 micro M, respectively. Pre-treatment of colon 26-L5 cells with evodiamine before inoculation into mice caused significant suppression of the liver metastasis as well as the lung metastasis. Lung metastasis by LLC is also inhibited significantly by pre-exposure to evodiamine. When the anti-migratory activity of evodiamine was compared with that of evodiamine-like compounds, rutaecarpine lacking a methyl group at N-14 and a hydrogen at C-13 b exhibited much less effect than evodiamine. In addition, reserpine, having beta-configurated hydrogen at C-13 b, inhibited tumor cell migration more potently than yohimbine, having alpha-configurated hydrogen at the same position. These results suggest that evodiamine may be useful as a leading compound for agents in tumor metastasis therapy. Also, the presence of a methyl group at N-14 and the configuration of hydrogen at C-13 b may be responsible for the inhibitory activities of evodiamine.  相似文献   

14.
The novel plasmin inhibitor YO-2, which also exerts an apoptosis-inducing effect on various human tumor cell cultures, was examined regarding its tumor growth inhibitory and antimetastatic action. The tumor growth inhibitory effect of YO-2 was studied using HT-29 human colon carcinoma, HT-18 human melanoma and HT-58 human B cell lymphoma inoculated as xenografts into immuno-deprived mice. Antimetastatic activity was tested on the B16 mouse melanoma muscle-lung model. YO-2 inhibited the growth of all xenografts in the range of 40-50%, when administered s.c. at a dose of 2.0 mg/kg (HT-29, HT-58) or orally at a dose of 0.4 mg/kg (HT-18). YO-2 decreased the number of lung metastasis found in mice inoculated i.m. with B16 melanoma, 4 mg/kg being the most effective. Since YO-2 is the only plasmin inhibitor having antihemorrhagic and also antitumor effect, this compound could be rationally used in combination therapy of neoplastic disease, especially when hemorrhage aggravates the course of the disease.  相似文献   

15.
Eucheuma serra agglutinin (ESA) is a lectin derived from a marine red alga E. serra and binds specifically to mannose-rich sugar chains. Previous reports have indicated that ESA associates with several cancer cells via sugar chains on cell surfaces and induces apoptotic cell death. In this study, we investigated the effect of ESA on Colon26 mouse colon adenocarcinoma cells both in vitro and in vivo. ESA induced cell death against Colon26 cells in vitro, and the expression of caspase-3 and the translocation of phosphatidylserine in ESA-treated Colon26 cells suggested that this cell death was induced through apoptosis. An intravenous injection of ESA significantly inhibited the growth of Colon26 tumors in BALB/c mice; moreover, DNA fragmentation was detected in tumor cells following ESA treatment. These results indicated that ESA is effective as an anti-cancer drug not only in vitro but also in vivo. The side-effects of ESA were not considered to be serious because the decrease in body weight of the mice injected with it was negligible. These observations suggest that ESA has the potential to be an effective anti-tumor drug.  相似文献   

16.
Antitumor activity of Klebsiella 03 lipopolysaccharide in mice   总被引:1,自引:0,他引:1  
The antitumor activity of Klebsiella 03 lipopolysaccharide (KO3 LPS) isolated from the culture supernatant against S180 sarcoma, Ehrlich carcinoma, MM2 mammary carcinoma and Meth A fibrosarcoma in mice was investigated. KO3 LPS significantly prolonged the lifespan of S180-bearing ddY mice and MM2-bearing C3H/He mice by intraperitoneal pre- or postmedication at doses ranging from 0.1 to 1.0 mg/kg. The LPS also inhibited the growth of subcutaneously inoculated Ehrlich carcinoma in ddY mice and Meth A sarcoma in BALB/c mice by intraperitoneal, intravenous or intratumoral administration. The intratumoral injection of KO3 LPS was most effective and results by the intravenous and the intraperitoneal administrations followed in effectiveness, but the administration through the subcutaneous route was hardly effective. Thus, KO3 LPS was shown to have antitumor activity on both allogeneic tumors and syngeneic tumors. It was also indicated in this study that the lifeprolonging effect of KO3 LPS on S180 ascites type tumor-bearing mice was significantly minimized by pretreatment of cyclophosphamide and that the LPS did not influence the cell viability of HeLa cells, Ehrlich cells and MM2 cells in vitro. These results suggest that the antitumor activity of KO3 LPS is provided by host-mediated actions.  相似文献   

17.
The mode of action of Nocardia rubra cell wall skeleton (N-CWS) on Meth A fibrosarcoma (Meth A) was studied in BALB/c mice. N-CWS suppressed or regressed the intradermal growth of syngeneic Meth A cells in normal BALB/c and athymic BALB/c mice. The intradermally and subcutaneously infiltrated cells harvested from injection sites of N-CWS in normal mice showed in vitro cytotoxic activity against Meth A cells. Pretreatment of normal BALB/c mice with immunosuppressing agents such as hydrocortisone, carrageenan, or silica particles significantly reduced the anti-tumor effect of N-CWS. The growth of Meth A cells, rechallenged into BALB/c mice in which Meth A cells had once been suppressed or regressed by N-CWS treatment, was also inhibited, but not in similarly treated athymic nude mice. This resistant mechanism was shown to be dependent out cellular components but not on humoral components by the Winn Assay. The present results suggest that N-CWS exerts its anti-tumor activity by mediation of the immune system of the host and that the main effector cells in the early stage of tumor rejection are macrophages; T cells may also be involved in the later stage.  相似文献   

18.
平阳霉素与单克隆抗体Fab′片段偶联物的抗肿瘤作用   总被引:7,自引:0,他引:7  
目的 研制一种以单抗Fab′片段为基础的抗肿瘤导向药物。方法 制备单抗3A5 Fab′片段及其与平阳霉素(PYM)偶联物Fab′-PYM后,测定Fab′-PYM与肿瘤细胞的免疫反应性、偶联物中PYM的抑菌活性、对肿瘤细胞的杀伤作用和体内抑瘤作用。结果 Fab′及Fab′-PYM保持了与靶细胞C26的免疫反应性;偶联物中PYM的抑菌活性为游离PYM的15%;Fab′-PYM对C26细胞的杀伤作用强于PYM;对非靶细胞KB的杀伤作用与PYM相似;ip和iv给药,Fab′-PYM对小鼠皮下接种的肠癌26生长抑制作用均强于3A5-PYM和PYM。结论 Fab′-PYM具有比PYM及3A5-PYM更强的体内外抗肿瘤作用。  相似文献   

19.
Single and combination chemotherapies of VP 16-213, a new antitumor agent were evaluated for its antitumor effect against several murine tumors. Dose-dependent antitumor effects were observed when VP 16-213 was administered via any of the three routes, i.p., i.v. and orally, on days 1 and 5 after i.p. or s.c. inoculation of Ehrlich carcinoma and sarcoma 180. The drug also proved effective against i.v. inoculated EL-LP-12 (subline of Ehrlich carcinoma), i.p. or s.c. inoculated P388 and B16 melanoma, i.p. inoculated colon 26, and s.c. inoculated colon 38 and Lewis lung carcinoma. However, oral administration of the drug was not effective against B16 melanoma, colon 26 and Lewis lung carcinoma despite the fact that the doses employed for this route was higher than those employed for i.v. and i.p. routes. The optimum dosing schedule was also investigated with oral administration of the drug against s.c. inoculated Ehrlich carcinoma. A single dose (day 1) or two doses (days 1 and 5) were more effective than three doses (days 1, 3 and 5) or five consecutive daily doses. VP 16-213 showed additive and more than additive effects in combination with the antitumor agents, cyclophosphamide, BCNU, mitomycin C or cisplatin against s.c. inoculated Ehrlich carcinoma and i.v. inoculated EL-LP-12.  相似文献   

20.
AIM: To investigate the antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide (GLPP). METHODS: Antitumor effect of GLPP was observed in tumor-bearing mice in vivo. At the same time,the effects of GLPP on proliferation of tumor cells and human umbilical cord vascular endothelial cell (HUVEC) were detected by MTr assay in vitro. Subsequently, spleen lymphocytes proliferation of nude mice was stimulated by LPS or ConA. To investigate the anti-angiogenic effect of GLPP, GLPP 80 μg per disc and GLPP-treated serum 10μL per disc were added to the chick chorioallantoic membrane (CAM) respectively in vivo. RESULTS: GLPP 50, 100, and 200 mg/kg inhibited growth of Sarcoma 180 in BALB/c mice markedly by 35.2 %, 45.2 %, and 61.9 %,respectively. GLPP which was directly added to the cultured medium did not inhibit PG cell proliferation in vitro;but GLPP-treated serum 50, 100, 200 mg/kg potently inhibited PG cell proliferation by 22.5 %, 26.8 %, and 30.3 %,respectively; and reduced the xenograft (human lung carcinoma cell PG) in BALB/c nude mice greatly in vivo by 55.5 %, 46.0 %, and 46.8 %, respectively. Lymphocytes proliferation of nude mice could be stimulated by LPS 5 mg/L but not by ConA 2.5 mg/L, indicating that GLPP could not promote the T lymphocyte proliferation and neutral red phagocytosis of peritoneal macrophages of nude mice. The CAM assay showed that GLPP and GLPP-treated serum had anti-angiogenic effect. GLPP (1, 10, and 100 mg/L) inhibited HUVEC proliferation in vitro with the inhibitory rate of 9.4 %, 15.6 %, and 40.4 %, respectively. CONCLUSION: GLPP has antitumor and antiangiogenic activity. The anti-angiogenesis of GLPP may be a new mechanism underlying its anti-tumor effects.  相似文献   

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