Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway

Stroke is an acute cerebrovascular disease caused by ruptured or blocked blood vessels.For the prevention of ischemic stroke,the coagulation state of blood and cerebrovascular protection should be considered.Our previous study has shown that salvianolic acid A(SAA),which is a water-soluble component from the root of Salvia Miltiorrhiza Bge,prevents thrombosis with a mild inhibitory effect on platelet aggregation.In this study we investigated the preventive effects of SAA on cerebrovascular endothelial injury caused by ischemia in vivo and oxygen-glucose deprivation(OGD)in vitro,and explored the underlying mechanisms.An autologous thrombus stroke model was established in SD rats by electrocoagulation.SAA(10 mg/kg)was orally administered twice a day for 5 days before the operation.The rats were sacrificed at 24 h after the operation.We showed that pretreatment with SAA significantly improved the neurological deficits,intracerebral hemorrhage,BBB disruption,and vascular endothelial dysfunction as compared with model group.In human brain microvascular endothelial cells(HBMECs),pretreatment with SAA(10μM)significantly inhibited OGD-induced cell viability reduction and degradation of tight junction proteins(ZO-1,occludin,claudin-5).Furthermore,we found that SAA inhibited the upregulation of Src signaling pathway in vivo and vitro and reversed the increased expression of matrix metalloproteinases(MMPs)after ischemic stroke.In conclusion,our results suggest that SAA protects cerebrovascular endothelial cells against ischemia and OGD injury via suppressing Src signaling pathway.These findings show that pretreatment with SAA is a potential therapeutic strategy for the prevention of ischemic stroke.     

苯扎溴铵溶液的稳定性观察

目的:评价在常温和0℃下存放的苯扎溴铵(新沽尔灭)的杀茵效果.方法:采用厂家3个批号的苯扎溴铵,分别于常温和0℃下存放24 h.稀释成1:50浓度后,将3种标准菌株置于其中,分别在作用5,10,15,30,60,120 min后取出药液,倾入培养基,置35℃培养箱培养48 h,观察菌株的存活情况.结果:经两种温度、不同时间的菌株培养结果观察,苯扎溴铵的杀菌效果一致,均能快速杀灭3种标准菌株.结论:苯扎溴铵消毒液保存在0℃下,不影响其杀菌效力,只要在保质期内,结冰的消毒液仍可使用,其性能稳定.     

青少年吸烟调查及影响因素分析

据世界卫生组织估计,目前全球每年约有5 000万人死于与吸烟相关的疾病[1].1996年全国吸烟流行病学调查结果显示,青少年的吸烟率升高,且开始吸烟的年龄提前[2].为了解商丘市青少年吸烟行为现状,为制定有效的干预措施提供依据,笔者对商丘市1 854名中学生的吸烟情况进行了调查.     

Neural circuits and nicotinic acetylcholine receptors mediate the cholinergic regulation of midbrain dopaminergic neurons and nicotine dependence

Midbrain dopaminergic(DA)neurons are governed by an endogenous cholinergic system,originated in the mesopontine nuclei.Nicotine hijacks nicotinic acetylcholine receptors(nAChRs)and interferes with physiological function of the cholinergic system.In this review,we describe the anatomical organization of the cholinergic system and the key nAChR subtypes mediating cholinergic regulation of DA transmission and nicotine reward and dependence,in an effort to identify potential targets for smoking intervention.Cholinergic modulation of midbrain DA systems relies on topographic organization of mesopontine cholinergic projections,and activation of nAChRs in midbrain DA neurons.Previous studies have revealed thatα4,α6,andβ2 subunit-containing nAChRs expressed in midbrain DA neurons and their terminals in the striatum regulatefirings of midbrain DA neurons and activity-dependent dopamine release in the striatum.These nAChRs undergo modification upon chronic nicotine exposure.Clinical investigation has demonstrated that partial agonists of these receptors elevate the success rate of smoking cessation relative to placebo.However,further investigations are required to refine the drug targets to mitigate unpleasant side-effects.     

春季食养贵在肝脾

主持人的话 过了立春和春节,大地开始逐渐由寒变暖.这就预示着春暖花开的季节即将来到了. 从立春到立夏前,包括立春、雨水、惊蛰、春分、清明、谷雨6个节气,是生发的季节.这段时间,春归大地,阳气生发,冰雪消融,蛰虫苏醒,自然界生机勃发,一派欣欣向荣的景象.此时养生,需适应季节变化,使机体与外界统一起来.中医称之为"春气之应,养生之道也".     

抗免疫缺陷病毒药物研发进展

病毒具有自我复制合成的特性,是一类最原始的在动物细胞内寄生的非细胞生物,自从1898年Beijerinck首次提出病毒概念后,病毒种类已由最初的几十种发展到今天的数千种,给人类健康带来巨大的危害.免疫缺陷病毒的滋生,使艾滋病(AIDS)患者每年递增,在发展中国家和贫穷落后的地区,由于缺乏有效的督导与控制,导致了感染率直线上升.     

由中、英、法药品监管现状的比较看我国的中药监管

为进一步了解英、法等发达国家的药品监管体系,借鉴其在药品监管信息化建设方面的成功经验,北京市药品监督管理局东城分局与北京市科学技术委员于不久前赴英国、法国实地考察,并与英国药理学会(British Pharmacological Society)、法国退休专家志愿者协会(France Volunteer Association of the Retired Experts)进行了深入的交流.     

抢救儿童有机磷中毒21例临床体会

<正>有机磷农药可因食入、吸入或皮肤吸收而中毒。小儿中毒原因多为:误食被有机磷农药污染的食物;误用污染农药的玩具或农药容器;母亲在使用农药后未认真洗手及换衣服而给婴儿哺乳,不恰     

对于自杀,一个就太多了

"活着"是每个人至高无上的权利,可有些人偏偏选择了放弃。9月是开学的日子,文文告别了父母兄妹,揣着东挪西凑的学费上了北京。她是乡里第一个考上北京名牌大学的学生,全乡人都以她为自豪。同宿舍的女生都来自城里,一个个时尚、新潮。看着她     

497例儿童中毒相关因素分析

目的:对儿童中毒病例进行分析、处理,找出儿童中毒原因及相关因素,寻求干预措施.方法:统计我院1997～2006年间收治住院的497名中毒儿童病案.结果:儿童中毒物质(药物和毒物)种类繁多,中毒以1～6岁儿童为主占77.26%,中毒原因74.25%为误服.结论:家长应加强对小儿的监管,妥善保管好药物和有毒物品,了解基本用药常识,警惕青春期孩子的心理动向,帮助其安全的度过疑惑期.