翼状胬肉切除联合自体角膜缘干细胞移植治疗翼状胬肉疗效观察

目的：观察翼状胬肉切除联合自体角膜缘干细胞移植对翼状胬肉治疗效果。方法：将98例翼状胬肉患者随机分为两组,观察组行翼状胬肉切除联合自体角膜缘干细胞移植术,对照组行单纯翼状胬肉切除术。结果：与对照组相比,观察组的角膜上皮愈合时间明显缩短,其结膜充血率、翼状胬肉复发率也降低,且二者比较差异具有统计学意义,P〈0.05。结论：翼状胬肉切除联合自体角膜缘干细胞移植术治疗翼状胬肉疗效确切,可以降低术后复发率。     

特大翼状胬肉切除术联合自体角膜缘干细胞移植术治疗翼状胬肉的临床应用分析

目的探讨特大翼状胬肉切除术联合自体角膜缘干细胞移植术治疗翼状胬肉的临床应用。方法选取我院收治的翼状胬肉患者100例(共136只患眼),依据治疗方法的不同,将其随机分为研究组与对照组,各50例。对照组给予单纯的翼状胬肉切除术,而研究组给予翼状胬肉切除术联合自体角膜缘干细胞移植术进行治疗。结果术后半年后,研究组与对照组的治愈率分别为92.86%、63.64%,组间比较差异显著(P<0.05);研究组的角膜上皮修复时间与住院时间明显优于对照组(P<0.05);两组治疗前后的泪膜破裂时间比较差异不显著(P>0.05);两组治疗后的角膜散光度均明显下降(P<0.05),但组间比较差异不显著(P>0.05)。结论特大翼状胬肉切除术联合自体角膜缘干细胞移植术治疗翼状胬肉的临床效果确切。     

翼状胬肉切除联合改良角膜缘干细胞移植术的临床评价

目的 评价翼状胬肉切除联合改良及常规角膜缘干细胞移植术的临床效果.方法 采用两种不同术式切除翼状胬肉,评价手术时间、术前及术后不同时间段患眼泪膜破裂时间(BUT),泪液分泌实验(SIt)以及患者的眼部不适症状.结果 改良组平均手术时间明显短于常规组,差异有统计学意义(P＜0.05);术后2周BUT改良组较常规组长,差异有统计学意义(P＜0.05);SIt改良组短于常规组,差异有统计学意义(P＜0.05);术后1周、2周改良组不适症状均轻于常规组,差异有统计学意义(P＜0.05).结论 翼状胬肉切除联合改良角膜缘干细胞移植术手术时间更短,术后不适症状更轻,泪膜功能恢复更快,是一种值得提倡和推广的手术方式.     

自体角膜缘上皮干细胞移植与单纯翼状胬肉切除术疗效比较

目的：观察翼状胬肉单纯切除术与翼状胬肉切除加自体角膜缘干细胞移植术的临床疗效。方法：将2006年3月～2010年6月78例（93眼）翼状胬肉患者随机分为翼状胬肉单纯切除术组（对照组）及翼状胬肉切除加自体角膜缘干细胞移植术组（治疗组）,比较其疗效。结果：采用翼状胬肉单纯切除术后患者复发率为13.3%,上皮修复时间为（5.83±0.84）d;而采用自体角膜缘干细胞移植后复发率仅为2.08%,上皮修复时间为（3.02±0.79）d,且两者相比,差异有统计学意义（P〈0.05）。结论：翼状胬肉切除加自体角膜缘干细胞移植术与翼状胬肉单纯切除术相比,可以有效地降低复发率,而且缩短角膜上皮修复时间,是治疗翼状胬肉较为理想有效的方法。     

自体角膜缘干细胞移植联合羊膜移植治疗复发性翼状胬肉的疗效观察

目的：探讨自体角膜缘干细胞移植联合羊膜移植术治疗复发性翼状胬肉的临床效果。方法选取60例（73眼）复发性翼状胬肉患者为研究对象,按照数字随机法分为观察组（30例,38眼）和对照组（30例,35眼）,对照组采用羊膜移植术,观察组采用自体角膜缘干细胞结膜植片联合羊膜移植术。术后随访12个月,分析比较两种术式的临床效果、复发率及并发症。结果两组患者的手术时间无明显差异（P〉0.05）,术后23周羊膜逐渐溶解、吸收。观察组角膜上皮愈合时间低于对照组（P〈0.05）;所有患者均获得随访,术后未出现角膜、巩膜溃疡,睑球粘连以及眼球活动受限等并发症。观察组2眼（5.3%）翼状胬肉复发,对照组4眼（11.4%）复发,观察组复发率显著低于对对照组（P〈0.05）,具有统计学意义。结论自体角膜缘干细胞移植联合羊膜移植治疗不会影响原术眼局部组织结构,可缩短角膜上皮愈合时间,降低翼状胬肉复发率。     

丝裂霉素C在治疗翼状胬肉中的临床应用

目的探讨丝裂霉素C在治疗翼状胬肉中的治疗效果。方法对382例(400眼)采用两种不同术式分两组行手术治疗。Ⅰ组:翼状胬肉切除术+自体角膜缘干细胞移植术;Ⅱ组:翼状胬肉切除术+自体角膜缘干细胞移植术+丝裂霉素C。经过6个月~5年的对比观察。结果两种术式比较:翼状胬肉切除术+自体角膜缘干细胞移植术119眼,治愈104眼,复发15眼,复发率12.6%;翼状胬肉切除术+自体角膜缘干细胞移植术+丝裂霉素C手术281眼,治愈276眼,复发5眼,复发率1.8%。结论翼状胬肉切除术+自体角膜缘干细胞移植术+丝裂霉素C治疗翼状胬肉复发率明显低于翼状胬肉切除术+自体角膜缘干细胞移植术,且术后角、结膜上皮修复快,视力恢复好。     

翼状胬肉显微切除加自体带角膜缘上皮结膜移植术的效果

目的:分析翼状胬肉显微切除加自体带角膜缘上皮结膜移植术的应用效果。方法:选取78例翼状胬肉患者,随机分组。对照组行传统翼状胬肉切除加自体带角膜缘上皮结膜移植术,观察组行翼状胬肉显微切除加自体带角膜缘上皮结膜移植术,比较两组治疗效果。结果:观察组治愈率明显高于对照组,角膜上皮修复时间和拆线时间均明显短于对照组(P0.05),术后并发症(异物感、创面水肿、结膜充血)发生率明显低于对照组(P0.05),两组复发率差异显著(P0.05)。结论:翼状胬肉显微切除加自体带角膜缘上皮结膜移植术治疗翼状胬肉效果理想,可缩短康复时间,术后并发症、疾病复发风险较低。     

综合护理在翼状胬肉切除联合自体角膜缘干细胞移植术患者中的应用

目的探究综合护理在翼状胬肉切除联合自体角膜缘干细胞移植术患者中的应用效果。方法选取翼状胬肉患者74例,均采用翼状胬肉切除术+自体角膜缘干细胞移植术治疗,其中37例接受常规护理,为对照组;另外37例接受综合护理,为观察组。比较分析两组患者的角膜上皮修复时间、拆线时间和并发症发生率。结果观察组角膜上皮修复时间和拆线时间均短于对照组,并发症发生率低于对照组,差异有统计学意义(P <0.05)。结论在翼状胬肉患者手术治疗中采用综合护理能够缩短术后康复时间,减少并发症的发生,在临床上存在显著的实践价值。     

用翼状胬肉切除术联合角膜缘干细胞移植术治疗翼状胬肉的效果分析

目的:分析翼状胬肉应用翼状胬肉切除术+角膜缘干细胞移植术治疗的临床效果。方法:对我院68例翼状胬肉患者资料进行分析,资料选取时间2016年4月-2018年4月,以治疗方案为分组原则,单一组(34例,应用翼状胬肉切除术治疗)、联合组(34例,应用翼状胬肉切除术+角膜缘干细胞移植术治疗),对比2组患者的总有效率、创面愈合时间、泪膜破裂时间及并发症发生率。结果:联合组患者总有效率94.12%比单一组76.47%高,创面愈合时间短于单一组,泪膜破裂时间长于单一组,并发症发生率为2.94%低于单一组17.65%,统计学对比差异性强(P0.05)。结论:翼状胬肉切除术+角膜缘干细胞移植术用于翼状胬肉治疗,疗效明显,并发症发生率低,患者症状改善好,恢复快,值得广泛应用。     

改良干细胞移植治疗翼状胬肉172例分析

目的 探讨翼状胬肉的手术治疗术式及疗效.方法 回顾2008年1月至2010年6月收治的翼状胬肉患者172例172眼,均行改良式角膜缘干细胞移植术,术后观察角膜上皮恢复情况、结膜伤口愈合情况和胬肉复发情况等.结果 所有患者术后随访3～24个月,绝大多数患者角膜上皮和结膜伤口愈合良好,有6例患者术后复发.结论 改良式角膜缘干细胞移植术治疗原发性翼状胬肉复发率低,可以广泛开展.     

Synthesis,Cytotoxicity and Antileishmanial Activity of Some N-(2-(indol-3-yl)ethyl)-7-chloroquinolin-4-amines

We report herein the condensation of 4,7-dichloroquinoline (1) with tryptamine (2) and D-tryptophan methyl ester (3) . Hydrolysis of the methyl ester adduct (5) yielded the free acid (6) . The compounds were evaluated in vitro for activity against four different species of Leishmania promastigote forms and for cytotoxic activity against Kb and Vero cells. Compound (5) showed good activity against the Leishmania species tested, while all three compounds displayed moderate activity in both Kb and Vero cells.     

Nachweis einiger Heilmittel in der Kniegelenksynovialflüssigkeit

Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren.     

Lung disease and PKCs

The lung offers a rich opportunity for development of therapeutic strategies focused on isozymes of protein kinase C (PKCs). PKCs are important in many cellular responses in the lung, and existing therapies for pulmonary disorders are inadequate. The lung poses unique challenges as it interfaces with air and blood, contains a pulmonary and systemic circulation, and consists of many cell types. Key structures are bronchial and pulmonary vessels, branching airways, and distal air sacs defined by alveolar walls containing capillaries and interstitial space. The cellular composition of each vessel, airway, and alveolar wall is heterogeneous. Injurious environmental stimuli signal through PKCs and cause a variety of disorders. Edema formation and pulmonary hypertension (PHTN) result from derangements in endothelial, smooth muscle (SM), and/or adventitial fibroblast cell phenotype. Asthma, chronic obstructive pulmonary disease (COPD), and lung cancer are characterized by distinctive pathological changes in airway epithelial, SM, and mucous-generating cells. Acute and chronic pneumonitis and fibrosis occur in the alveolar space and interstitium with type 2 pneumocytes and interstitial fibroblasts/myofibroblasts playing a prominent role. At each site, inflammatory, immune, and vascular progenitor cells contribute to the injury and repair process. Many strategies have been used to investigate PKCs in lung injury. Isolated organ preparations and whole animal studies are powerful approaches especially when genetically engineered mice are used. More analysis of PKC isozymes in normal and diseased human lung tissue and cells is needed to complement this work. Since opposing or counter-regulatory effects of selected PKCs in the same cell or tissue have been found, it may be desirable to target more than one PKC isozyme and potentially in different directions. Because multiple signaling pathways contribute to the key cellular responses important in lung biology, therapeutic strategies targeting PKCs may be more effective if combined with inhibitors of other pathways for additive or synergistic effect. Mechanisms that regulate PKC activity, including phosphorylation and interaction with isozyme-specific binding proteins, are also potential therapeutic targets. Key isotypes of PKC involved in lung pathophysiology are summarized and current and evolving therapeutic approaches to target them are identified.     

Gender-related symptoms and correlates of alcohol dependence among men and women with a lifetime diagnosis of alcohol use disorders

This study explored gender-related symptoms and correlates of alcohol dependence in a crosssectional study of 150 men and 150 women with a lifetime diagnosis of alcohol use disorders (AUD). Participants were recruited in equal numbers from treatment settings, correctional centres and the general community. Standardized measures were used to determine participants' use of substances, history of psychiatric disorders and psychosocial stress, their sensation seeking and family history of substance use and mental health disorders. Multivariate analyses were used to detect patterns of variables associated with gender and the lifetime severity of AUD. Men had a longer history of severe AUD than women. Women had similar levels of alcohol dependence and medical and psychological sequelae as men, despite 6 fewer years of AUD. More women than men had a history of severe psychosocial stress, severe dependence on other substances and antecedent mental health problems, especially mood and anxiety disorders. There were differences in family history of alcohol-related problems approximating same-gender aggregation. The severity of a lifetime AUD was predicted by its earlier age at onset and the occurrence of other disorders, especially anxiety, among both men and women. The limitations in the generalizability of these findings due to sample idiosyncrasies are discussed.     

Biochemical and molecular characterisation of cubozoan protein toxins

Class Cubozoa includes several species of box jellyfish that are harmful to humans. The venoms of box jellyfish are stored and discharged by nematocysts and contain a variety of bioactive proteins that are cytolytic, cytotoxic, inflammatory or lethal. Although cubozoan venoms generally share similar biological activities, the diverse range and severity of effects caused by different species indicate that their venoms vary in protein composition, activity and potency. To date, few individual venom proteins have been thoroughly characterised, however, accumulating evidence suggests that cubozoan jellyfish produce at least one group of homologous bioactive proteins that are labile, basic, haemolytic and similar in molecular mass (42-46 kDa). The novel box jellyfish toxins are also potentially lethal and the cause of cutaneous pain, inflammation and necrosis, similar to that observed in envenomed humans. Secondary structure analysis and remote protein homology predictions suggest that the box jellyfish toxins may act as α-pore-forming toxins. However, more research is required to elucidate their structures and investigate their mechanism(s) of action. The biological, biochemical and molecular characteristics of cubozoan venoms and their bioactive protein components are reviewed, with particular focus on cubozoan cytolysins and the newly emerging family of box jellyfish toxins.     

Dose tolerability of chronically inhaled voriconazole solution in rodents

Invasive pulmonary aspergillosis (IPA) is a fungal disease of the lung associated with high mortality rates in immunosuppressed patients despite treatment. Targeted drug delivery of aqueous voriconazole solutions has been shown in previous studies to produce high tissue and plasma drug concentrations as well as improved survival in a murine model of IPA. In the present study, rats were exposed to 20 min nebulizations of normal saline (control group) or aerosolized aqueous solutions of voriconazole at 15.625 mg (low dose group) or 31.25 mg (high dose group). Peak voriconazole concentrations in rat lung tissue and plasma after 3 days of twice daily dosing in the high dose group were 0.85 ± 0.63 μg/g wet lung weight and 0.58 ± 0.30 μg/mL, with low dose group lung and plasma concentrations of 0.38 ± 0.01 μg/g wet lung weight and 0.09 ± 0.06 μg/mL, respectively. Trough plasma concentrations were low but demonstrated some drug accumulation over 21 days of inhaled voriconazole administered twice daily. Following multiple inhaled doses, statistically significant but clinically irrelevant abnormalities in laboratory values were observed. Histopathology also revealed an increase in the number of alveolar macrophages but without inflammation or ulceration of the airway, interstitial changes, or edema. Inhaled voriconazole was well tolerated in a rat model of drug inhalation.