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1.
龙眼参中多糖的含量测定   总被引:6,自引:0,他引:6  
目的 :测定龙眼参中多糖的含量。方法 :以苯酚—硫酸比色法测定 ,λm ax=490 nm。结果 :五批龙眼参中的多糖含量分别为 0 .2 4%、0 .2 3 %、0 .2 4%、0 .2 5 %和 0 .2 4%。结论 :龙眼参中的多糖含量较高 ,平均为 0 .2 4± 0 .0 0 71% (n=5 )。  相似文献   

2.
目的 :建立二味康中多糖的含量测定方法。方法 :用苯酚 硫酸比色法测定多糖的含量 ,测定波长 488nm。结果 :本法线性范围为 6~ 6 0 μg·ml- 1,平均回收率 =97.94%,RSD =2 .33%(n =6 )。测得 5批样品中多糖含量分别为 56 .7,6 8.4,73.1 ,73.4,90 .4mg·ml- 1。结论 :该法可用于二味康中多糖的含量测定。  相似文献   

3.
参麦注射液治疗冠心病30例临床分析   总被引:1,自引:0,他引:1  
参麦注射液 (5 0 ml/安瓶 )是由雅安三九药业有限公司采用新技术从红参、麦冬中提取 ,含有人参皂甙、麦冬皂甙、麦冬黄酮及微量人参多糖和麦冬多糖组成的中药制剂。我院自1998年 8月至 2 0 0 0年 8月 ,用参麦注射液观察治疗 30例冠心病人 ,现报告如下。1 资料及方法1.1 病例选择 :选自我院就诊病人 ,按 WHO1978年诊断标准诊断为冠心病心绞痛型 ,无心脏扩大、左室肥厚者 6 0例 ,男 40例 ,女 2 0例 ;年龄 5 4~ 80岁 ,平均 6 2岁。按随机分配原则 ,分为治疗组 30例 ,对照组 30例。1.2 给药方法 :治疗组用参麦注射液 10 0 ml加于 10 %葡萄…  相似文献   

4.
注射用卡提素冻干粉针中核糖与多糖的含量测定   总被引:1,自引:0,他引:1  
目的  建立注射用卡提素中核糖与多糖含量测定的方法。 方法  采用紫外分光光度法 ,在 6 5 0nm波长处测定核糖的含量 ,在 6 2 0nm波长处测定多糖的含量。 结果  核糖的平均回收率为 10 0 2 7% ,RSD为0 88%。多糖的平均回收率为 10 0 30 % ,RSD为 0 96 %。 结论  此方法可行。  相似文献   

5.
3,5-二硝基水杨酸法测二味康口服液中多糖的含量   总被引:17,自引:0,他引:17  
目的 :建立二味康中多糖含量测定方法。方法 :采用 3 ,5 二硝基水扬酸法 (简称DNS法 )对制剂中多糖进行含量测定。结果 :样品经DNS试剂显色后于 5 2 0nm处测定吸收度 ,在此波长处溶液的吸光度与葡萄糖含量呈良好线性关系 ,线性范围 :0 .0 0 8~ 0 .0 40g·L- 1 ,r =0 .9998,加样回收率为 10 0 .7% ,RSD为 2 .2 % (n =6) ;测得 9批样品 ,多糖含量以葡萄糖计为14 .0 3~ 2 2 .91g·L- 1 。结论 :DNS法用于测定多糖含量简单易行 ,且重复性好 ,可作为该制剂常规分析方法。  相似文献   

6.
天芎注射液中多糖的含量测定   总被引:7,自引:0,他引:7  
目的 建立天芎注射液中多糖含量的测定方法。方法 硫酸蒽酮作为显色液,采用比色法测定天芎注射液中多糖的含量。结果 线性范围为10~5 0 μg·ml-1(r=0 . 9999) ;高、中、低浓度平均回收率为98. 7% ,RSD =0 .83% (n =6 )。结论 所用方法操作简便,重复性好,适用于天芎注射液中多糖的含量测定。  相似文献   

7.
目的 研究对墨西哥仙人掌的粗多糖的提取及糖含量的测定。方法 酒精沉淀法提取粗多糖 ,利用分光光度法测定糖含量。结果 沉淀 48~ 6 0h ,酒精用量 6 5倍 ,为提取仙人掌粗多糖方法。仙人掌酸提物的糖含量 46 19%,水提物的糖含量为 43 5 2 %。结论 酸提物中粗多糖的阿拉伯糖含量高于水提物中粗多糖的阿拉伯糖含量。  相似文献   

8.
目的 建立利福定胶囊的含量测定方法。方法 采用KromasilC18柱 (4 .6mm× 2 0 0mm ,5 μm) ,以甲醇 - 0 0 5mol·L-1乙酸铵溶液为流动相 ,进样 2 0 μl,检测波长 2 5 4nm。 结果 利福定在 0 0 5~ 0 5 4mg·ml-1范围内 ,呈良好的线性关系 (r =0 9999) ,平均回收率为 10 0 6 6 % ,RSD =0 82 %。结论 方法准确可靠 ,可用于测定利福定胶囊的含量  相似文献   

9.
RP-HPLC法测定复方甲硝唑片中甲硝唑和维生素B_6的含量   总被引:1,自引:0,他引:1  
目的 :建立RP HPLC法测定复方甲硝唑片中甲硝唑和维生素B6的含量。方法 :采用Shim packCLC ODS柱 (4 .6mm× 2 0 0mm ,5 μm) ,甲醇 水 (30∶70 )为流动相 ,2 90nm为检测波长 ,外标法测定含量。 结果 :甲硝唑和维生素B6的线性范围分别为 5 0~ 5 0 0 μg·ml-1(r=0 .9996 ) ,5~ 5 0 μg·ml-1(r =0 .9998) ,平均回收率分别为 10 0 .2 % (RSD =0 .93% ) ,99.5 %(RSD =0 .4 2 % ) ,n =6。结论 :RP HPLC法测定复方甲硝唑片中甲硝唑和维生素B6的含量 ,方法准确 ,操作简便 ,结果可靠。  相似文献   

10.
参芪胶囊及其原药材中多糖的含量测定   总被引:2,自引:0,他引:2  
目的测定参芪胶囊及原药材黄芪、党参、甘草中多糖的含量。方法用硫酸苯酚法测定多糖含量。结果参芪胶囊的多糖含量为 0 .95g/ 10粒 ;原药材黄芪、党参、甘草中的多糖含量分别为 6 .97%、2 3.83%、2 .4 4 %。结论参芪胶囊中多糖含量略低于等量原药材中多糖含量 (黄芪 3g、党参 3g、甘草 2g,理论多糖含量 0 .97g)之和。测定方法可行。  相似文献   

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13.
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.  相似文献   

14.
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.  相似文献   

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16.
Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.
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This article assesses pain within the context of the dose response. A substantial number of studies indicate that the dose response for pain-related endpoints is commonly biphasic, being independent of the type of biological model employed, endpoint measured, or agent tested. The quantitative features of the dose response are also remarkably consistent regardless of the receptor pathway that mediates the nociceptive response, indicating a likely downstream message convergence. These findings have important implications for drug discovery, development, and clinical evaluation.  相似文献   

19.
Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren.  相似文献   

20.
The current USP National Formulary contains 65 Monographs for drug formulations containing neomycin. All 65 Monographs prescribe a bioassay for neomycin assay. This bioassay, based on cell culture, is labor intensive, has poor precision, and cannot be adapted for purity or identification. High-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPAE-IPAD), a liquid chromatography technique, has been shown to be suitable for neomycin purity analysis and neomycin assay of an over-the-counter first aid cream (Hanko and Rohrer [17]). Here we propose that an HPAE-IPAD assay can replace the bioassay in the 65 neomycin-containing Monographs. We applied the HPAE-IPAD assay to four neomycin-containing drug products representing the four classes of formulations found in the 65 Monographs, liquid, solid, suspension, and cream. Each drug was analyzed with two chromatography systems, and on 3 separate days. For all products, HPAE-IPAD measurements were precise and accurate with respect to the label concentrations. There was also high accuracy for spike recovery of neomycin from the four drug products throughout 70–150% of the labeled concentration. These results suggest that an HPAE-IPAD assay would be an accurate assay for neomycin, and would be faster and more precise than the current bioassay.  相似文献   

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