天然产物分离中高速逆流色谱溶剂体系的研究进展

根据溶剂体系的极性与分离的天然产物的关系,本文对高速逆流色谱中使用的溶剂系统进行了归纳总结,不仅对快速选择溶剂体系分离天然产物起到了一定的指导作用,而且为今后高速逆流色谱溶剂体系的研究提供了方便。     

高效制备液相色谱在天然产物分离中的应用

综述高效制备液相色谱法在天然产物有效成分分离中的应用研究进展。用高效制备液相色谱法分离制备天然产物的单体,具有高效、操作方便、快速的优点,当其与高速逆流色谱、大孔吸附树脂及超临界CO2萃取联用时,分离效果更好。     

高速逆流色谱的原理及活性成份提取的进展

综述HSCCC的原理及从天然产物中提取活性成份的应用及进展:高速逆流色谱(high-speed countercurrent chroma-tography,HSCCC)是新型的液-液分配色谱技术。HSCCC利用多层螺旋管同步行星式离心运动,在短时间内实现样品在互不相溶的两相溶剂系统中的高效分配,从而实现样品分离。HSCCC在天然产物的分离方面具有很大的优势,此项技术已被应用于生化、生物工程、医药、天然产物化学、有机合成、环境分析、食品、地质、材料等领域。     

高速逆流色谱在中药现代化研究中的应用

中药复杂成分体系的科学阐述是中药现代化研究中的重中之重和难点所在。高速逆流色谱是最近20多年发展起来的新型液-液分配技术,是解决中药及天然产物中化学成分方面的一项新兴的关键技术。简要介绍高速逆流色谱在中药现代化研究中如成分分离(单体分离、系统成分分离、标准品或对照品制备等)、质量控制、药物筛选以及中药复方的研究进展。     

高速逆流色谱在天然产物分离中的应用

综述了高速逆流色谱在天然产物有效成分分离中的新应用.     

pH区带精制逆流色谱在分离生物碱类物质的应用

pH区带精制逆流色谱法是在普通制备型高速逆流色谱分离纯化生物碱类成分的应用基础上发展起来的一种特殊逆流色谱分离制备技术。pH区带精制逆流色谱法由于具备进样量高、分离效率高、分离效果好、杂质易收集、可实现pH监控、待分离组分被高度浓缩等优点,目前已被广泛应用于研究领域与实践中。生物碱类物质是重要的天然产物成分,具有很强的生物活性,对很多疾病有良好的治疗作用。本文介绍了pH区带精制逆流色谱法及其在分离生物碱类天然产物中的应用。     

高速逆流色谱技术在药物和天然产物分离中的应用

综述了高速逆流色谱技术在药物和天然产物分离中的新应用.     

高速逆流色谱技术在植物活性成分分离与纯化中的应用

高速逆流色谱是一种连续液-液色谱技术,具有无固相载体、样品无需严格预处理等优点。本文就近年来高速逆流色谱在植物有效成分分离方面的应用作了简述。     

高效逆流色谱研究进展——在天然产物有效成分分离方面的应用

本文简述了HSCCC近年来在天然产物有效成分分离方面的应用,介绍了双向逆流色谱、正交轴逆流色谱、pH-区带精制逆流色谱和HSCCC与MS联用技术。     

高效逆流色谱研究进展——在天然产物有效成分分离方面的应用

本文简述了HSCCC近年来在天然产物有效成分分离方面的应用,介绍了双向逆流色谱、正交轴逆流色谱、pH-区带精制逆流色谱和HSCCC与MS联用技术.     

超临界流体色谱在药物分析中的研究进展

超临界流体色谱是以超临界流体为流动相,依靠流动相的溶剂化能力来进行分离分析的一种色谱,具有分离速度快、效能高、绿色环保等优点,是气相色谱和液相色谱的有力补充,具有广阔的应用前景。本文利用中国知网（CNKI）、ScienceDirect、PubMed等数据库检索了近几年来超临界流体色谱在药物分析中的研究文献,归纳了其在手性药物、天然产物、药物代谢和杂质检测中的应用进展,并对今后的进一步研究提出思考。     

The ethics of dietary supplements and natural health products in pharmacy practice: A systematic documentary analysis

Objectives Many natural health products and dietary supplements are purchased in pharmacies and it has been argued that pharmacists are in the best position to provide patients with evidence‐based information about them. This study was designed to identify how the pharmacist's role with respect to natural health products and dietary supplements is portrayed in the literature. Method A systematic search was conducted in a variety of health databases to identify all literature that pertained to both pharmacy and natural health products and dietary supplements. Of the 786 articles identified, 665 were broad‐coded and 259 were subjected to in‐depth qualitative content analysis for emergent themes. Key findings Overwhelmingly, support for the sale of natural health products and dietary supplements in pharmacies is strong. Additionally, a role for pharmacist counselling is underscored. But another recurrent theme is that pharmacists are ill‐equipped to counsel patients about these products that are available on their shelves. This situation has led some to question the ethics of pharmacists selling natural health products and dietary supplements and to highlight the existence of an ethical conflict stemming from the profit‐motive associated with sales of natural health products and dietary supplements. Conclusions This analysis raises concerns about the ethics of natural health products and dietary supplements being sold in pharmacies, and about pharmacists being expected to provide counselling about products of which they have little knowledge.     

Natural product inhibitors of the ubiquitin-proteasome pathway

Natural products continue to be a source of inspiration for chemists and biologists alike. The search for biologically active natural prodcuts has provided troves of information about biological processes, and natural products continue to be some of the most powerful and useful probes of biological processes available. Complex and unusual molecular architectures provide the impetus for new reaction development and push the limits of known synthetic chemistry. In addition to serving as tools for basic research, natural products represent starting points for drug discovery efforts in many cases. This review details the impressive chemical diversity present in the family of molecules that inhibit the proteasome and other aspects of the ubiquitin-proteasome pathway. To date, over 60 natural products that inhibit the ubiquitin-proteasome pathway have been identified. Although early work recognized inhibitors of the proteasome itself, more modern research has revealed that inhibition of many aspects of this critical pathway is possible. One powerful example of this is the inhibition of the interaction between p53 and MDM2 (an E3 ubiquitin ligase). Natural product proteasome inhibitors have served as powerful tools to unravel the intricacies of the ubiquitin-proteasome and related pathways. Several of these natural products have been developed into anticancer drug candidates, and one proteasome inhibitor has already been approved for the treatment of multiple myeloma. Despite the wealth of information available about naturally occurring proteasome inhibitors and related compounds, it is clear that exciting research about this class of molecules will continue well into the future.     

东北红豆杉及其伤愈组织粗提物中紫杉醇的HPLC-ESI-MS/MS分析研究

目的研究紫杉醇的二级质谱裂解规律，对东北红豆杉及其伤愈组织粗提物中的紫杉醇进行HPLC-ESI-MS/MS分离定性，为天然产物中该化合物的分离分析提供一种快速可靠的方法。方法对HPLC-ESI-MS/MS条件进行优化，研究紫杉醇电喷雾质谱的一级电离规律和二级质谱裂解规律，并结合液相色谱的保留时间判断东北红豆杉及其伤愈组织粗提物中紫杉醇色谱峰的归属。结果详细阐明了紫杉醇的二级质谱裂解规律，并利用HPLC-ESI-MS/MS法准确快速地分离定性了两种粗提物中紫杉醇。结论本方法灵敏度高，快速，选择性高，专属性好，能够作为天然产物及其产品中紫杉醇分离定性的一种快速可靠的技术手段。